Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,393,854	A→G	L802S (TTA→TCA)	yhdP ←	AsmA2 domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,393,854	0	A	G	100.0%	318.1 / NA	85	L802S (TTA→TCA)	yhdP	AsmA2 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (37/48);  total (37/48)

TGACCGAGCAACCAGCGGCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTCAATGCTTCGTTCACCGCTTCAGGC  >  NC_000913/3393719‑3393988                                                                                                                                        |                                                                                                                                       tGACCGAGCAACCAGCGGCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTg                                                                                                                                    >  1:67628/1‑140 (MQ=255)      gAGCAACCAGCGGCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGc                                                                                                                               <  2:423594/140‑1 (MQ=255)      gAGCAACCAGCGGCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGc                                                                                                                               <  1:901594/140‑1 (MQ=255)       aGCAACCAGCGGCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCt                                                                                                                              >  2:85970/1‑140 (MQ=255)           aCCAGCGGCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTcaca                                                                                                                          <  2:67628/140‑1 (MQ=255)              aGCGGCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTc                                                                                                                       >  1:209846/1‑140 (MQ=255)                  gCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTa                                                                                                                   >  2:331641/1‑140 (MQ=255)                  gCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTa                                                                                                                   <  1:1084568/140‑1 (MQ=255)                  gCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTa                                                                                                                   >  2:1682977/1‑140 (MQ=255)                  gCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTa                                                                                                                   >  2:72143/1‑140 (MQ=255)                   cTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTca                                                                                                                            >  2:1690901/1‑130 (MQ=255)                   cTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTca                                                                                                                            <  1:1690901/130‑1 (MQ=255)                      ttGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTca                                                                                                                            >  2:552404/1‑127 (MQ=255)                      ttGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTca                                                                                                                            <  1:552404/127‑1 (MQ=255)                        gAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGc                                                                                                             >  1:425329/1‑140 (MQ=255)                           aTGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGt                                                                                                          <  1:156589/140‑1 (MQ=255)                           aTGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGt                                                                                                          >  2:1515783/1‑140 (MQ=255)                            tGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGtt                                                                                                         <  1:274877/140‑1 (MQ=255)                            tGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGtt                                                                                                         <  2:1752109/140‑1 (MQ=255)                              aTTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCa                                                                                                       >  1:740924/1‑140 (MQ=255)                               ttATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCAg                                                                                                      <  2:463603/140‑1 (MQ=255)                               ttATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCAg                                                                                                      <  1:1391719/140‑1 (MQ=255)                               ttATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCAg                                                                                                      <  2:209846/140‑1 (MQ=255)                                tATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGc                                                                                                     >  2:907137/1‑140 (MQ=255)                                tATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGc                                                                                                     >  1:620223/1‑140 (MQ=255)                                 aTCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGct                                                                                                    <  1:1433031/140‑1 (MQ=255)                                 aTCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGct                                                                                                    <  1:717064/140‑1 (MQ=255)                                    cGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTa                                                                                                 >  1:136223/1‑140 (MQ=255)                                      cACCAGCCTGTCCGGCTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATg                                                                                               >  2:1024488/1‑140 (MQ=255)                                       aCCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGtt                                                                                                         <  2:101495/129‑1 (MQ=255)                                       aCCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGtt                                                                                                         >  1:101495/1‑129 (MQ=255)                                       aCCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGt                                                                                              >  1:765061/1‑140 (MQ=255)                                        ccAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCAg                                                                                                      >  2:1057811/1‑131 (MQ=255)                                        ccAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCAg                                                                                                      <  1:1057811/131‑1 (MQ=255)                                         cAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTta                                                                                            <  2:765061/140‑1 (MQ=255)                                         cAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTta                                                                                            <  2:1437988/140‑1 (MQ=255)                                         cAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTta                                                                                            >  2:1225731/1‑140 (MQ=255)                                          aGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTtat                                                                                           >  1:372231/1‑140 (MQ=255)                                              tGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGt                                                                                       >  1:673965/1‑140 (MQ=255)                                              tGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGt                                                                                       <  1:1077491/140‑1 (MQ=255)                                                  cGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCa                                                                                   <  2:620223/140‑1 (MQ=255)                                                     ttAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCa                                                                                >  1:673772/1‑140 (MQ=255)                                                      tAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTta                                                                                            >  1:36030/1‑127 (MQ=255)                                                      tAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTta                                                                                            <  2:36030/127‑1 (MQ=255)                                                      tAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAg                                                                               <  2:425329/140‑1 (MQ=255)                                                      tAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAg                                                                               <  1:907137/140‑1 (MQ=255)                                                      tAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAg                                                                               <  1:85970/140‑1 (MQ=255)                                                      tAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAg                                                                               <  1:641415/140‑1 (MQ=255)                                                          tCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATg                                                                           >  2:566789/1‑140 (MQ=255)                                                           cAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGa                                                                          <  2:673772/140‑1 (MQ=255)                                                           cAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGa                                                                          <  2:136223/140‑1 (MQ=255)                                                                          tGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCa                                                              <  2:1460977/137‑1 (MQ=255)                                                                          tGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCa                                                              >  1:1460977/1‑137 (MQ=255)                                                                           gCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATGc                                                          <  1:72143/140‑1 (MQ=255)                                                                            ccATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATGcc                                                         <  1:1144124/140‑1 (MQ=255)                                                                            ccATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATGcc                                                         <  1:1072954/140‑1 (MQ=255)                                                                             cATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATGccc                                                        >  1:230106/1‑140 (MQ=255)                                                                             cATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATGccc                                                        >  1:996505/1‑140 (MQ=255)                                                                              aTCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATGCCCa                                                       <  2:344692/140‑1 (MQ=255)                                                                              aTCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATGCCCa                                                       <  2:673965/140‑1 (MQ=255)                                                                              aTCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATGCCCa                                                       <  2:230106/140‑1 (MQ=255)                                                                              aTCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATGCCCa                                                       <  2:912169/140‑1 (MQ=255)                                                                              aTCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATGCCCa                                                       <  2:1355504/140‑1 (MQ=255)                                                                                      tAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATGCCCACTTTACCa                                               >  1:559477/1‑140 (MQ=255)                                                                                              aCCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATGCCCACTTTACCATCccacgc                                       >  1:262805/1‑140 (MQ=255)                                                                                              aCCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATGCCCACTTTACCATCccacgc                                       >  1:1701212/1‑140 (MQ=255)                                                                                               ccGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATGCCCACTTTACCATCccacgcc                                      <  2:262805/140‑1 (MQ=255)                                                                                               ccGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATGCCCACTTTACCATCccacgcc                                      <  2:1701212/140‑1 (MQ=255)                                                                                                cGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATGCCCACTTTACCATCccacgcca                                     <  2:740924/140‑1 (MQ=255)                                                                                                  cTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATGCCCACTTTACCATCccacgccacg                                   <  1:1007147/140‑1 (MQ=255)                                                                                                     gTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATGCCCACTTTACCATCCCACGCCACGCTg                                <  1:1044853/140‑1 (MQ=255)                                                                                                     gTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATGCCCACTTTACCATCCCACGCCACGCTg                                >  2:547275/1‑140 (MQ=255)                                                                                                        gTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATGCCCACTTTACCATCccac                                         >  2:858845/1‑128 (MQ=255)                                                                                                        gTTCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATGCCCACTTTACCATCccac                                         <  1:858845/128‑1 (MQ=255)                                                                                                          tCACCCGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATGCCCACTTTACCATCCCACGCCACGCTGCCACt                           <  2:372231/140‑1 (MQ=255)                                                                                                             cccGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTCaa                        >  1:262600/1‑140 (MQ=255)                                                                                                               cGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTCAATg                      <  2:559477/140‑1 (MQ=255)                                                                                                               cGCAGGTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTCAATg                      >  1:427048/1‑140 (MQ=255)                                                                                                                   ggTTTGGCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTCAATGCTTc                  >  2:433195/1‑140 (MQ=255)                                                                                                                        ggCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTCAATGCTTCGTTCa             <  2:427048/140‑1 (MQ=255)                                                                                                                        ggCTAACGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTCAATGCTTCGTTCa             <  2:996505/140‑1 (MQ=255)                                                                                                                             aCGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTCAATGCTTCGTTCACCGCt        <  1:1682977/140‑1 (MQ=255)                                                                                                                              cGGTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTCAATGCTTCGTTCACCGCtt       >  1:1537111/1‑140 (MQ=255)                                                                                                                               ggTGAAGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGTAGATCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTCAATGCTTCGTTCACCGCTTc      <  1:331641/140‑1 (MQ=255)                                                                                                                                   aaGGTGAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTCAATGCTTCGTTCACCGCTTCAGGc  >  1:805515/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                       TGACCGAGCAACCAGCGGCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTCAATGCTTCGTTCACCGCTTCAGGC  >  NC_000913/3393719‑3393988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑