Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,566,757	C→A	D164Y (GAT→TAT)	yjiN ←	DUF445 domain‑containing protein YjiN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,566,757	0	C	A	100.0%	281.3 / NA	79	D164Y (GAT→TAT)	yjiN	DUF445 domain‑containing protein YjiN
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (42/37);  total (42/37)

TGCTGGGCGATAAACTTGCGCGATTTATCGCGCTGGAGAAGGGCGATCAACTGTGCGATCAGCGTATCCAGCAGCACCTGATGACGATCGTTTTTGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATCGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCTGAATACGCGCATCATCGGTAAGTTCGAGAAAACCGCTCATGATCTGCAACAGATGCTGACCAACGCGGCGGGCGTTTTCTGGCTGGCTA  >  NC_000913/4566621‑4566890                                                                                                                                         |                                                                                                                                      tGCTGGGCGATAAACTTGCGCGATTTATCGCGCTGGAGAAGGGCGATCAACTGTGCGATCAGCGTATCCAGCAGCACCTGATGACGATCGTTTTTGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGAc                                                                                                                                    >  1:541137/1‑140 (MQ=255) tGCTGGGCGATAAACTTGCGCGATTTATCGCGCTGGAGAAGGGCGATCAACTGTGCGATCAGCGTATCCAGCAGCACCTGATGACGATCGTTTTTGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGAc                                                                                                                                    <  1:1034940/140‑1 (MQ=255)       gCGATAAACTTGCGCGATTTATCGCGCTGGAGAAGGGCGATCAACTGTGCGATCAGCGTATCCAGCAGCACCTGATGACGATCGTTTTTGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATc                                                                                                                              >  2:1239722/1‑140 (MQ=255)        cGATAAACTTGCGCGATTTATCGCGCTGGAGAAGGGCGATCAACTGTGCGATCAGCGTATCCAGCAGCACCTGATGACGATCGTTTTTGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATCa                                                                                                                             >  2:1619917/1‑140 (MQ=255)           tAAACTTGCGCGATTTATCGCGCTGGAGAAGGGCGATCAACTGTGCGATCAGCGTATCCAGCAGCACCTGATGACGATCGTTTTTGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATCAATc                                                                                                                          <  1:1647153/140‑1 (MQ=255)                       aTTTATCGCGCTGGAGAAGGGCGATCAACTGTGCGATCAGCGTATCCAGCAGCACCTGATGACGATCGTTTTTGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGAcc                                                                                                              >  1:770889/1‑140 (MQ=255)                       aTTTATCGCGCTGGAGAAGGGCGATCAACTGTGCGATCAGCGTATCCAGCAGCACCTGATGACGATCGTTTTTGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATCAAT‑GCCCGATGGACcg                                                                                                             <  2:349651/140‑1 (MQ=255)                        tttATCGCGCTGGAGAAGGGCGATCAACTGTGCGATCAGCGTATCCAGCAGCACCTGATGACGATCGTTTTTGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACcg                                                                                                             <  1:1613188/140‑1 (MQ=255)                        tttATCGCGCTGGAGAAGGGCGATCAACTGTGCGATCAGCGTATCCAGCAGCACCTGATGACGATCGTTTTTGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACcg                                                                                                             <  2:770889/140‑1 (MQ=255)                                  tGGAGAAGGGCGATCAACTGTGCGATCAGCGTATCCAGCAGCACCTGATGACGATCGTTTTTGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAg                                                                                                   >  2:1650180/1‑140 (MQ=255)                                    gagaAGGGCGATCAACTGTGCGATCAGCGTATCCAGCAGCACCTGATGACGATCGTTTTTGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCa                                                                                                 <  1:529001/140‑1 (MQ=255)                                          ggCGATCAACTGTGCGATCAGCGTATCCAGCAGCACCTGATGACGATCGTTTTTGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCt                                                                                           >  1:517842/1‑140 (MQ=255)                                          ggCGATCAACTGTGCGATCAGCGTATCCAGCAGCACCTGATGACGATCGTTTTTGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCt                                                                                           <  1:1566266/140‑1 (MQ=255)                                              aTCAACTGTGCGATCAGCGTATCCAGCAGCACCTGATGACGATCGTTTTTGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAg                                                                                                <  2:335127/131‑1 (MQ=255)                                              aTCAACTGTGCGATCAGCGTATCCAGCAGCACCTGATGACGATCGTTTTTGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAg                                                                                                >  1:335127/1‑131 (MQ=255)                                              aTCAACTGTGCGATCAGCGTATCCAGCAGCACCTGATGACGATCGTTTTTGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGgcg                                                                                             >  1:1155214/1‑134 (MQ=255)                                              aTCAACTGTGCGATCAGCGTATCCAGCAGCACCTGATGACGATCGTTTTTGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGgcg                                                                                             <  2:1155214/134‑1 (MQ=255)                                              aTCAACTGTGCGATCAGCGTATCCAGCAGCACCTGATGACGATCGTTTTTGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCTGAAt                                                                                       >  2:1028546/1‑140 (MQ=255)                                              aTCAACTGTGCGATCAGCGTATCCAGCAGCACCTGATGACGATCGTTTTTGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCTGAAt                                                                                       >  1:1646742/1‑140 (MQ=255)                                              aTCAACTGTGCGATCAGCGTATCCAGCAGCACCTGATGACGATCGTTTTTGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCTGAAt                                                                                       >  2:544487/1‑140 (MQ=255)                                                   cTGTGCGATCAGCGTATCCAGCAGCACCTGATGACGATCGTTTTTGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCTGAATAcgcg                                                                                  <  2:357737/140‑1 (MQ=255)                                                     gtgCGATCAGCGTATCCAGCAGCACCTGATGACGATCGTTTTTGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCTGAATACGCGca                                                                                <  1:1619917/140‑1 (MQ=255)                                                      tgCGATCAGCGTATCCAGCAGCACCTGATGACGATCGTTTTTGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCTGAATACGCGcat                                                                               >  1:1742555/1‑140 (MQ=255)                                                       gCGATCAGCGTATCCAGCAGCACCTGATGACGATCGTTTTTGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCTGAATACGCGcatc                                                                              <  2:1316708/140‑1 (MQ=255)                                                       gCGATCAGCGTATCCAGCAGCACCTGATGACGATCGTTTTTGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCTGAATACGCGcatc                                                                              <  1:1012205/140‑1 (MQ=255)                                                        cGATCAGCGTATCCAGCAGCACCTGATGACGATCGTTTTTGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCTGAATACGCGcatca                                                                             >  1:1521677/1‑140 (MQ=255)                                                             aGCGTATCCAGCAGCACCTGATGACGATCGTTTTTGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCTGaa                                                                                        >  1:1771596/1‑124 (MQ=255)                                                             aGCGTATCCAGCAGCACCTGATGACGATCGTTTTTGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCTGaa                                                                                        <  2:1771596/124‑1 (MQ=255)                                                             aGCGTATCCAGCAGCACCTGATGACGATCGTTTTTGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCTGAATACGCGCATCATCGGt                                                                        <  1:1028546/140‑1 (MQ=255)                                                             aGCGTATCCAGCAGCACCTGATGACGATCGTTTTTGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCTGAATACGCGCATCATCGGt                                                                        <  2:1521677/140‑1 (MQ=255)                                                             aGCGTATCCAGCAGCACCTGATGACGATCGTTTTTGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCTGAATACGCGCATCATCGGt                                                                        >  2:1446853/1‑140 (MQ=255)                                                             aGCGTATCCAGCAGCACCTGATGACGATCGTTTTTGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCTGAATACGCGCATCATCGGt                                                                        >  2:270860/1‑140 (MQ=255)                                                             aGCGTATCCAGCAGCACCTGATGACGATCGTTTTTGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCTGAATACGCGCATCATCGGt                                                                        <  1:544487/140‑1 (MQ=255)                                                             aGCGTATCCAGCAGCACCTGATGACGATCGTTTTTGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCTGAATACGCGCATCATCGGt                                                                        <  2:517842/140‑1 (MQ=255)                                                             aGCGTATCCAGCAGCACCTGATGACGATCGTTTTTGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCTGAATACGCGCATCATCGGt                                                                        <  1:1650180/140‑1 (MQ=255)                                                             aGCGTATCCAGCAGCACCTGATGACGATCGTTTTTGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCTGAATACGCGCATCATCGGt                                                                        >  1:284393/1‑140 (MQ=255)                                                             aGCGTATCCAGCAGCACCTGATGACGATCGTTTTTGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCCGAATACGCGca                                                                                >  1:1469376/1‑132 (MQ=255)                                                             aGCGTATCCAGCAGCACCTGATGACGATCGTTTTTGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCCGAATACGCGca                                                                                <  2:1469376/132‑1 (MQ=255)                                                                gTATCCAGCAGCACCTGATGACGATCGTTTTTGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCTGAATACGCGCATCATCGGTAAg                                                                     <  1:1126404/140‑1 (MQ=255)                                                                gTATCCAGCAGCACCTGATGACGATCGTTTTTGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCTGAATACGCGCATCATCGGTAAg                                                                     >  2:1126404/1‑140 (MQ=255)                                                                gTATCCAGCAGCACCTGATGACGATCGTTTTTGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCTGAATACGCGCATCATCGGTAAg                                                                     >  2:713382/1‑140 (MQ=255)                                                                       gcagcaCCTGATGACGATCGTTTTTGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCTGAATACGCGCATCATCGGTAAGTTCgaga                                                              <  1:1239722/140‑1 (MQ=255)                                                                       gcagcaCCTGATGACGATCGTTTTTGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCTGAATACGCGCATCATCGGTAAGTTCgaga                                                              >  2:116634/1‑140 (MQ=255)                                                                         agcaCCTGATGACGATCGTTTTTGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCTGAATACGCGCATCATCGGTAAGTTCGAGaaa                                                            <  1:27610/140‑1 (MQ=255)                                                                          gcaCCTGATGACGATCGTTTTTGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCTGAATACGCGCATCATCGGTAAGTTCGAGaaaa                                                           <  1:270860/140‑1 (MQ=255)                                                                           caCCTGATGACGATCGTTTTTGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCTGAATACGCGCATCATCGGTAAGTTCGAGAAAAc                                                          >  2:1233929/1‑140 (MQ=255)                                                                             ccTGATGACGATCGTTTTTGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCTGAATACGCGCATCATCGGTAAGTTCGAGAAAACCg                                                        >  2:711008/1‑140 (MQ=255)                                                                             ccTGATGACGATCGTTTTTGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCTGAATACGCGCATCATCGGTAAGTTCGAGAAAACCg                                                        >  1:335292/1‑140 (MQ=255)                                                                                 atgaCGATCGTTTTTGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCTGAATACGCGCATCATCGGTAAGTTCGAGAAAACCGCTCa                                                    <  1:1446853/140‑1 (MQ=255)                                                                                  tgaCGATCGTTTTTGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCTGAATACGCGCATCATCGGTAAGTTCGAGAAAACCGCTCAt                                                   >  1:859571/1‑140 (MQ=255)                                                                                  tgaCGATCGTTTTTGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCTGAATACGCGCATCATCGGTAAGTTCGAGAAAACCGCTCAt                                                   >  1:727905/1‑140 (MQ=255)                                                                                   gaCGATCGTTTTTGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCTGAATACGCGCATCATCGGTAAGTTCGAGAAAACCGCTCATg                                                  <  2:284393/140‑1 (MQ=255)                                                                                   gaCGATCGTTTTTGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCTGAATACGCGCATCATCGGTAAGTTCGAGAAAACCGCTCATg                                                  <  2:412090/140‑1 (MQ=255)                                                                                       aTCGTTTTTGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCTGAATACGCGCATCATCGGTAAGTTCGAGAAAACCGCTCATGATCt                                              <  1:46301/140‑1 (MQ=255)                                                                                        tCGTTTTTGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCTGAATACGCGCATCATCGGTAAGTTCGAGAAAACCGCTCATGATCTg                                             >  2:973638/1‑140 (MQ=255)                                                                                        tCGTTTTTGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCTGAATACGCGCATCATCGGTAAGTTCGAGAAAACCGCTCATGATCTg                                             <  2:642079/140‑1 (MQ=255)                                                                                        tCGTTTTTGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCTGAATACGCGCATCATCGGTAAGTTCGAGAAAACCGCTCATGATCTg                                             >  1:849058/1‑140 (MQ=255)                                                                                           tttttGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCTGAATACGCGCATCATCGGTAAGTTCGAGAAAACCGCTCATGATCTGCaa                                          >  2:1662620/1‑140 (MQ=255)                                                                                            ttttGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCTGAATACGCGCATCATCGGTAAGTTCGAGAAAACCGCTCATGATCTGCAAc                                         <  1:1662620/140‑1 (MQ=255)                                                                                            ttttGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCTGAATACGCGCATCATCGGTAAGTTCGAGAAAACCGCTCATGATCTGCAAc                                         <  1:711008/140‑1 (MQ=255)                                                                                             tttGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCTGAATACGCGCATCATCGGTAAGTTCGAGAAAACCGCTCATGATCTGCAACa                                        >  1:684186/1‑140 (MQ=255)                                                                                               tGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCTGAATACGCGCATCATCGGTAAGTTCGAGAAAACCGCTCATGATCTGCAACAGa                                      >  2:1296687/1‑140 (MQ=255)                                                                                                      aCTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCTGAATACGCGCATCATCGGTAAGTTCGAGAAAACCGCTCATGATCTGCAACAGATGCTGAc                               >  1:995926/1‑140 (MQ=255)                                                                                                      aCTCTCCAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCTGAATACGCGCATCATCGGTAAGTTCGAGAAAACCGCTCATGATCTGCAACAGATGCTGAc                               >  2:113940/1‑140 (MQ=255)                                                                                                            cAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCTGAATACGCGCATCATCGGTAAGTTCGAGAAAACCGCTCATGATCTGCAACAGATGCTGACCAAcgc                         >  1:1547909/1‑140 (MQ=255)                                                                                                            cAGCATCAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCTGAATACGCGCATCATCGGTAAGTTCGAGAAAACCGCTCATGATCTGCAACAGATGCTGACCAAcgc                         >  2:1070410/1‑140 (MQ=255)                                                                                                               cATCAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCTGAATACGCGCATCATCGGTAAGTTCGAGAAAACCGCTCATGATCTGCAACAGATGCTGACCAACgcggc                      >  1:468466/1‑140 (MQ=255)                                                                                                                 tCAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCTGAATACGCGCATCATCGGTAAGTTCGAGAAAACCGCTCATGATCTGCAACAGATGCTGACCAACGcggcgg                    >  2:1770432/1‑140 (MQ=255)                                                                                                                  cAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCTGAATACGCGCATCATCGGTAAGTTCGAGAAAACCGCTCATGATCTGCAACAGATGCTGACCAACGCggcggg                   <  1:113940/140‑1 (MQ=255)                                                                                                                  cAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCTGAATACGCGCATCATCGGTAAGTTCGAGAAAACCGCTCATGATCTGCAACAGATGCTGACCAACGCggcggg                   <  1:1770432/140‑1 (MQ=255)                                                                                                                  cAACGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCTGAATACGCGCATCATCGGTAAGTTCGAGAAAACCGCTCATGATCTGCAACAGATGCTGACCAACGCggcggg                   <  1:148361/140‑1 (MQ=255)                                                                                                                    aCGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCTGAATACGCGCATCATCGGTAAGTTCGAGAAAACCGCTCATGATCTGCAACAGATGCTGACCAACGCggcgggcg                 >  2:505141/1‑140 (MQ=255)                                                                                                                    aCGCACTGGTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCTGAATACGCGCATCATCGGTAAGTTCGAGAAAACCGCTCATGATCTGCAACAGATGCTGACCAACGCggcgggcg                 >  1:731276/1‑140 (MQ=255)                                                                                                                            gTGCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCTGAATACGCGCATCATCGGTAAGTTCGAGAAAACCGCTCATGATCTGCAACAGATGCTGACCAACGCGGCGGGCGTTTTctgg         >  1:1091989/1‑140 (MQ=255)                                                                                                                              gCCGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCTGAATACGCGCATCATCGGTAAGTTCGAGAAAACCGCTCATGATCTGCAACAGATGCTGACCAACGCGGCGGGCGTTTTctggct       <  2:468466/140‑1 (MQ=255)                                                                                                                               ccGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCTGAATACGCGCATCATCGGTAAGTTCGAGAAAACCGCTCATGATCTGCAACAGATGCTGACCAACGCGGCGGGCGTTTTctggctg      >  2:1031365/1‑140 (MQ=255)                                                                                                                               ccGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCTGAATACGCGCATCATCGGTAAGTTCGAGAAAACCGCTCATGATCTGCAACAGATGCTGACCAACGCGGCGGGCGTTTTctggctg      <  2:859571/140‑1 (MQ=255)                                                                                                                               ccGGAAAGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCTGAATACGCGCATCATCGGTAAGTTCGAGAAAACCGCTCATGATCTGCAACAGATGCTGACCAACGCGGCGGGCGTTTTctggctg      >  1:1556728/1‑140 (MQ=255)                                                                                                                                   aaaGATAGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCTGAATACGCGCATCATCGGTAAGTTCGAGAAAACCGCTCATGATCTGCAACAGATGCTGACCAACGCGGCGGGCGTTTTCTGGCTGGCTa  <  2:849058/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                      TGCTGGGCGATAAACTTGCGCGATTTATCGCGCTGGAGAAGGGCGATCAACTGTGCGATCAGCGTATCCAGCAGCACCTGATGACGATCGTTTTTGGTCATACTCTCCAGCATCAACGCACTGGTGCCGGAAAGATCGACCTTATCAATCGCCCGATGGACCGCGCGCTTAAGCAGGCGCTGAATACGCGCATCATCGGTAAGTTCGAGAAAACCGCTCATGATCTGCAACAGATGCTGACCAACGCGGCGGGCGTTTTCTGGCTGGCTA  >  NC_000913/4566621‑4566890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑