Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	275,977	C→A	S218* (TCG→TAG)	mmuP →	S‑methyl‑L‑methionine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	275,977	0	C	A	100.0%	281.5 / NA	80	S218* (TCG→TAG)	mmuP	S‑methyl‑L‑methionine transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (42/38);  total (42/38)

GGCGGCGATTTTCGGCTTTATTCCGATGCAGGATGGCTCGCCCGCGCCGGGGCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGGCTTACCG‑ATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCTGGCAGCGCT  >  NC_000913/275840‑276109                                                                                                                                           |                                                                                                                                     ggcggcGATTTTCGGCTTTATTCCGATGCAGGATGGCTCGCCCGCGCCGGGGCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGGCTTACCG‑ATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTAgg                                                                                                                                    >  1:1533950/1‑140 (MQ=255) ggcggcGATTTTCGGCTTTATTCCGATGCAGGATGGCTCGCCCGCGCCGGGGCTGAGTAATATCACGGCAGAAG‑‑TGGTTCCCGCACGGTGGCTTACCG‑ATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTAGGGt                                                                                                                                  >  1:317715/1‑140 (MQ=255)   cggcgATTTTCGGCTTTATTCCGATGCAGGATGGCTCGCCCGCGCCGGGGCTGAGTAATATCACGGCAGAAG‑‑TGGTTCCCGCACGGTGGCTTACCG‑ATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTAGGGTAc                                                                                                                                <  2:317715/140‑1 (MQ=255)      cgATTTTCGGCTTTATTCCGATGCAGGATGGCTCGCCCGCGCCGGGGCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGGCTTACCG‑ATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTAGGGTAcc                                                                                                                               >  1:1110349/1‑140 (MQ=255)      cgATTTTCGGCTTTATTCCGATGCAGGATGGCTCGCCCGCGCCGGGGCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGGCTTACCG‑ATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTAGGGTAcc                                                                                                                               >  2:1118433/1‑140 (MQ=255)       gATTTTCGGCTTTATTCCGATGCAGGATGGCTCGCCCGCGCCGGGGCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGGCTTACCG‑ATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTAGGGTACCg                                                                                                                              <  1:794767/140‑1 (MQ=255)       gATTTTCGGCTTTATTCCGATGCAGGATGGCTCGCCCGCGCCGGGGCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGGCTTACCG‑ATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTAGGGTACCg                                                                                                                              <  1:1405221/140‑1 (MQ=255)                    aTTCCGATGCAGGATGGCTCGCCCGCGCCGGGGCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGGCTTACCG‑ATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCAt                                                                                                                 <  1:409902/140‑1 (MQ=255)                          aTGCAGGATGGCTCGCCCGCGCCGGGGCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGGCTTACCG‑ATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTgccgc                                                                                                           >  1:1034226/1‑140 (MQ=255)                          aTGCAGGATGGCTCGCCCGCGCCGGGGCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGGCTTACCG‑ATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTgccgc                                                                                                           >  2:246768/1‑140 (MQ=255)                          aTGCAGGATGGCTCGCCCGCGCCGGGGCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGGCTTACCG‑ATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTgccgc                                                                                                           <  2:731260/140‑1 (MQ=255)                          aTGCAGGATGGCTCGCCCGCGCCGGGGCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGGCTTACCG‑ATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTgccgc                                                                                                           >  2:461754/1‑140 (MQ=255)                              aGGATGGCTCGCCCGCGCCGGGGCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGGCTTACCG‑ATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGt                                                                                                       <  2:264878/140‑1 (MQ=255)                               ggATGGCTCGCCCGCGCCGGGGCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGGCTTACCG‑ATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTg                                                                                                      >  2:347055/1‑140 (MQ=255)                                     cTCGCCCGCGCCGGGGCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGGCTTACCG‑ATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACgg                                                                                                >  1:793354/1‑140 (MQ=255)                                               ccGGGGCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGGCTTACCG‑ATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCcgcg                                                                                      <  2:458619/140‑1 (MQ=255)                                               ccGGGGCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGGCTTACCG‑ATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCcgcg                                                                                      <  1:237255/140‑1 (MQ=255)                                               ccGGGGCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGGCTTACCG‑ATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCcgcg                                                                                      <  1:30488/140‑1 (MQ=255)                                                cGGGGCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGGCTTACCG‑ATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCgcgc                                                                                     <  2:793354/140‑1 (MQ=255)                                                     cTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGGCTTACCG‑ATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGt                                                                                <  2:64459/140‑1 (MQ=255)                                                        aGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGGCTTACCG‑ATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTAt                                                                             >  1:81325/1‑140 (MQ=255)                                                          tAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGGCTTACCGAATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATc                                                                            >  1:1197712/1‑140 (MQ=255)                                                          tAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGGCTTACCG‑ATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATcc                                                                           <  1:742936/140‑1 (MQ=255)                                                               tCACGGCAGAAGGCTGGTTCCCGCACGGTGGCTTACCG‑ATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTa                                                                      <  1:277465/140‑1 (MQ=255)                                                                cACGGCAGAAGGCTGGTTCCCGCACGGTGGCTTACCG‑ATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAg                                                                     <  1:1110080/140‑1 (MQ=255)                                                                cACGGCAGAAGGCTGGTTCCCGCACGGTGGCTTACCG‑ATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAg                                                                     >  2:135477/1‑140 (MQ=255)                                                                  cGGCAGAAGGCTGGTTCCCGCACGGTGGCTTACCG‑ATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCg                                                                   >  1:1619828/1‑140 (MQ=255)                                                                  cGGCAGAAGGCTGGTTCCCGCACGGTGGCTTACCG‑ATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCg                                                                   >  1:1111011/1‑140 (MQ=255)                                                                  cGGCAGAAGGCTGGTTCCCGCACGGTGGCTTACCG‑ATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCg                                                                   >  2:1264229/1‑140 (MQ=255)                                                                  cGGCAGAAGGCTGGTTCCCGCACGGTGGCTTACCG‑ATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCg                                                                   <  2:899124/140‑1 (MQ=255)                                                                  cGGCAGAAGGCTGGTTCCCGCACGGTGGCTTACCG‑ATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCg                                                                   <  2:179657/140‑1 (MQ=255)                                                                  cGGCAGAAGGCTGGTTCCCGCACGGTGGCTTACCG‑ATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCg                                                                   >  2:227278/1‑140 (MQ=255)                                                                   ggCAGAAGGCTGGTTCCCGCACGGTGGCTTACCG‑ATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGa                                                                  <  2:1034226/140‑1 (MQ=255)                                                                   ggCAGAAGGCTGGTTCCCGCACGGTGGCTTACCG‑ATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGa                                                                  <  2:284330/140‑1 (MQ=255)                                                                   ggCAGAAGGCTGGTTCCCGCACGGTGGCTTACCG‑ATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGa                                                                  <  1:1643311/140‑1 (MQ=255)                                                                    gCAGAAGGCTGGTTCCCGCACGGTGGCTTACCG‑ATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGAt                                                                 >  2:1011082/1‑140 (MQ=255)                                                                     cAGAAGGCTGGTTCCCGCACGGTGGCTTACCG‑ATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGAtt                                                                <  1:227278/140‑1 (MQ=255)                                                                     cAGAAGGCTGGTTCCCGCACGGTGGCTTACCG‑ATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGAtt                                                                <  2:1261880/140‑1 (MQ=255)                                                                     cAGAAGGCTGGTTCCCGCACGGTGGCTTACCG‑ATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGAtt                                                                <  1:1264229/140‑1 (MQ=255)                                                                     cAGAAGGCTGGTTCCCGCACGGTGGCTTACCG‑ATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTAGGGAACGGAGCTTATCGGCATTGCCGCCGGGGAAACGGAAAATCCGCGCAAAGTTATCCCGGTAGCGAtt                                                                >  2:1146413/1‑140 (MQ=255)                                                                       gAAGGCTGGTTCCCGCACGGTGGCTTACCG‑ATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGAtt                                                                <  1:1341770/138‑1 (MQ=255)                                                                       gAAGGCTGGTTCCCGCACGGTGGCTTACCG‑ATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGAtt                                                                >  2:1341770/1‑138 (MQ=255)                                                                       gAAGGCTGGTTCCCGCACGGTGGCTTACCG‑ATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCg                                                              <  2:913494/140‑1 (MQ=255)                                                                        aaGGCTGGTTCCCGCACGGTGGCTTACCG‑ATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGt                                                             >  2:1051029/1‑140 (MQ=255)                                                                         aGGCTGGTTCCCGCACGGTGGCTTACCG‑ATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGta                                                            <  1:135477/140‑1 (MQ=255)                                                                               gTTCCCGCACGGTGGCTTACCG‑ATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCa                                                      >  2:775296/1‑140 (MQ=255)                                                                               gTTCCCGCACGGTGGCTTACCG‑ATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCa                                                      <  1:1118433/140‑1 (MQ=255)                                                                                   ccGCACGGTGGCTTACCGAATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATcg                                                   <  2:1197712/140‑1 (MQ=255)                                                                                    cGCACGGTGGCTTACCG‑ATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGAtt                                                                >  1:1320991/1‑125 (MQ=255)                                                                                    cGCACGGTGGCTTACCG‑ATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGAtt                                                                <  2:1320991/125‑1 (MQ=255)                                                                                    cGCACGGTGGCTTACCG‑ATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATcgcg                                                 >  1:921854/1‑140 (MQ=255)                                                                                     gCACGGTGGCTTACCG‑ATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGAt                                                                 <  2:497791/123‑1 (MQ=255)                                                                                     gCACGGTGGCTTACCG‑ATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGAt                                                                 >  1:497791/1‑123 (MQ=255)                                                                                      cACGGTGGCTTACCG‑ATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATcgcgcg                                               >  1:303575/1‑140 (MQ=255)                                                                                        cGGTGGCTTACCG‑ATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGAc                                             <  2:81325/140‑1 (MQ=255)                                                                                          gTGGCTTACCG‑ATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTg                                           >  2:884076/1‑140 (MQ=255)                                                                                           tGGCTTACCG‑ATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGa                                          >  2:910301/1‑140 (MQ=255)                                                                                           tGGCTTACCG‑ATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGa                                          <  1:1470395/140‑1 (MQ=255)                                                                                             gCTTACCG‑ATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGatt                                        >  2:1507818/1‑140 (MQ=255)                                                                                                   cG‑ATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTc                                  >  1:1005985/1‑140 (MQ=255)                                                                                                        tttGATGACTATGGTGGCAGTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTa                              <  2:1533950/140‑1 (MQ=255)                                                                                                        tttGATGACTATGGTGGCAGTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTa                              >  1:1741722/1‑140 (MQ=255)                                                                                                         ttGATGACTATGGTGGCAGTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTAt                             <  1:775296/140‑1 (MQ=255)                                                                                                          tgatgaCTATGGTGGCAGTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATc                            <  1:347055/140‑1 (MQ=255)                                                                                                             tgaCTATGGTGGCAGTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGc                         >  1:430840/1‑140 (MQ=255)                                                                                                              gaCTATGGTGGCAGTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCa                        <  1:246768/140‑1 (MQ=255)                                                                                                                   tggtggCAGTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCgtg                   >  2:593102/1‑140 (MQ=255)                                                                                                                    ggtggCAGTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCgtgt                  >  2:404139/1‑140 (MQ=255)                                                                                                                      tggCAGTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGttt                >  1:644945/1‑140 (MQ=255)                                                                                                                        gCAGTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTtgt              >  1:1158028/1‑140 (MQ=255)                                                                                                                         cAGTGAACTTTGCTTTTTATGGTACCGATCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTtgtg             >  1:578083/1‑140 (MQ=255)                                                                                                                         cAGTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTtgtg             >  2:1585578/1‑140 (MQ=255)                                                                                                                           gTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCt           >  2:1235648/1‑140 (MQ=255)                                                                                                                           gTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCt           >  1:521121/1‑140 (MQ=255)                                                                                                                           gTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCt           >  2:336384/1‑140 (MQ=255)                                                                                                                           gTGAACTTTGCTTTTTAGGGTACCGAGCTTATCGGCAATGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCt           >  2:564688/1‑140 (MQ=255)                                                                                                                              aaCTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCTGGc        >  1:1434925/1‑140 (MQ=255)                                                                                                                               aCTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCTGGCa       <  2:579415/140‑1 (MQ=255)                                                                                                                               aCTTTGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCTGGCa       <  2:1741722/140‑1 (MQ=255)                                                                                                                                  ttGCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCTGGCAgcg    >  2:1451187/1‑140 (MQ=255)                                                                                                                                    gCTTTTTAGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCTGGCAGCGCt  <  2:1005985/140‑1 (MQ=255)                                                                                                                                           |                                                                                                                                     GGCGGCGATTTTCGGCTTTATTCCGATGCAGGATGGCTCGCCCGCGCCGGGGCTGAGTAATATCACGGCAGAAGGCTGGTTCCCGCACGGTGGCTTACCG‑ATTTTGATGACTATGGTGGCAGTGAACTTTGCTTTTTCGGGTACCGAGCTTATCGGCATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCTGGCAGCGCT  >  NC_000913/275840‑276109

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑