Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	293,318	G→T	L201I (CTT→ATT)	yagL ←	CP4‑6 prophage; resolvase‑like catalytic domain‑containing protein YagL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	293,318	0	G	T	100.0%	340.1 / NA	95	L201I (CTT→ATT)	yagL	CP4‑6 prophage; resolvase‑like catalytic domain‑containing protein YagL
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (40/55);  total (40/55)

ATGGCTGTTAAAAAGTTACTCTGCAATTAGCTTCTGGAATTAACCGATGATTCCGTTATTCCAGTGCCGCTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAAGTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAACATGTCGATATCGGCTTTTAGTTGATTTCAGATTGTGAATGTCAATCGTATCCTGACGAGCAATCAAAAAAGACAAGCAA  >  NC_000913/293181‑293454                                                                                                                                          |                                                                                                                                         aTGGCTGTTAAATAGTTACTCTGCAATTAGCTTCTGGAATTAACCGATGATTCCGTTATTCCAGTGCCGCTTCACAGTCGATAAACTGATACTCAATTTCTTTTCAATCTCAGCCTGATTAAATCCCCCTTTCTTAATTa                                                                                                                                        <  2:306229/140‑1 (MQ=255)  tGGCTGTTAAAAAGTTACTCTGCAATTAGCTTCTGGAATTAACCGATGATTCCGTTATTCCAGTGCCGCTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTAt                                                                                                                                       <  1:1085894/140‑1 (MQ=255)    gCTGTTAAAAAGTTACTCTGCAATTAGCTTCTGGAATTAACCGATGATTCCGTTATTCCAGTGCCGCTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATtg                                                                                                                                     >  1:698250/1‑140 (MQ=255)    gCTGTTAAAAAGTTACTCTGCAATTAGCTTCTGGAATTAACCGATGATTCCGTTATTCCAGTGCCGCTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATtg                                                                                                                                     >  2:1609244/1‑140 (MQ=255)     cTGTTAAAAAGTTACTCTGCAATTAGCTTCTGGAATTAACCGATGATTCCGTTATTCCAGTGCCGCTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATtgt                                                                                                                                    >  2:914669/1‑140 (MQ=255)     cTGTTAAAAAGTTACTCTGCAATTAGCTTCTGGAATTAACCGATGATTCCGTTATTCCAGTGCCGCTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATtgt                                                                                                                                    >  1:1257525/1‑140 (MQ=255)      tGTTAAAAAGTTACTCTGCAATTAGCTTCTGGAATTAACCGATGATTCCGTTATTCCAGTGCCGCTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATtgtg                                                                                                                                   >  2:1451333/1‑140 (MQ=255)       gTTAAAAAGTTACTCTGCAATTAGCTTCTGGAATTAACCGATGATTCCGTTATTCCAGTGCCGCTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGa                                                                                                                                  >  2:651050/1‑140 (MQ=255)        ttAAAAAGTTACTCTGCAATTAGCTTCTGGAATTAACCGATGATTCCGTTATTCCAGTGCCGCTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGat                                                                                                                                 <  1:914669/140‑1 (MQ=255)         tAAAAAGTTACTCTGCAATTAGCTTCTGGAATTAACCGATGATTCCGTTATTCCAGTGCCGCTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGata                                                                                                                                <  2:1503857/140‑1 (MQ=255)         tAAAAAGTTACTCTGCAATTAGCTTCTGGAATTAACCGATGATTCCGTTATTCCAGTGCCGCTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGata                                                                                                                                <  1:1670601/140‑1 (MQ=255)          aaaaaGTTACTCTGCAATTAGCTTCTGGAATTAACCGATGATTCCGTTATTCCAGTGCCGCTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGatat                                                                                                                               <  2:1055687/140‑1 (MQ=255)             aaGTTACTCTGCAATTAGCTTCTGGAATTAACCGATGATTCCGTTATTCCAGTGCCGCTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCAc                                                                                                                            <  1:528010/140‑1 (MQ=255)               gTTACTCTGCAATTAGCTTCTGGAATTAACCGATGATTCCGTTATTCCAGTGCCGCTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCGCGt                                                                                                                          <  1:962529/140‑1 (MQ=255)               gTTACTCTGCAATTAGCTTCTGGAATTAACCGATGATTCCGTTATTCCAGTGCCGCTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCACGt                                                                                                                          <  1:143809/140‑1 (MQ=255)               gTTACTCTGCAATTAGCTTCTGGAATTAACCGATGATTCCGTTATTCCAGTGCCGCTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCACGt                                                                                                                          <  2:1257525/140‑1 (MQ=255)                ttACTCTGCAATTAGCTTCTGGAATTAACCGATGATTCCGTTATTCCAGTGCCGCTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCACGTc                                                                                                                         >  1:680721/1‑140 (MQ=255)                 tACTCTGCAATTAGCTTCTGGAATTAACCGATGATTCCGTTATTCCAGTGCCGCTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCACGTCg                                                                                                                        <  2:1438891/140‑1 (MQ=255)                 tACTCTGCAATTAGCTTCTGGAATTAACCGATGATTCCGTTATTCCAGTGCCGCTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAAACCACCTTTCTTAATTATTGTGATATCACGTCg                                                                                                                        <  2:985625/140‑1 (MQ=255)                  aCTCTGCAATTAGCTTCTGGAATTAACCGATGATTCCGTTATTCCAGTGCCGCTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAattatt                                                                                                                                      <  1:694818/125‑1 (MQ=255)                  aCTCTGCAATTAGCTTCTGGAATTAACCGATGATTCCGTTATTCCAGTGCCGCTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAattatt                                                                                                                                      >  2:694818/1‑125 (MQ=255)                  aCTCTGCAATTAGCTTCTGGAATTAACCGATGATTCCGTTATTCCAGTGCCGCTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATtg                                                                                                                                     <  2:584944/126‑1 (MQ=255)                  aCTCTGCAATTAGCTTCTGGAATTAACCGATGATTCCGTTATTCCAGTGCCGCTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATtg                                                                                                                                     >  1:584944/1‑126 (MQ=255)                  aCTCTGCAATTAGCTTCTGGAATTAACCGATGATTCCGTTATTCCAGTGCCGCTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCACg                                                                                                                           >  1:181261/1‑136 (MQ=255)                  aCTCTGCAATTAGCTTCTGGAATTAACCGATGATTCCGTTATTCCAGTGCCGCTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCACg                                                                                                                           <  2:181261/136‑1 (MQ=255)                   ctctGCAATTAGCTTCTGGAATTAACCGATGATTCCGTTATTCCAGTGCCGCTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCACGTCGGt                                                                                                                      <  1:1089811/140‑1 (MQ=255)                   ctctGCAATTAGCTTCTGGAATTAACCGATGATTCCGTTATTCCAGTGCCGCTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCACGTCGGt                                                                                                                      <  2:2687/140‑1 (MQ=255)                     ctGCAATTAGCTTCTGGAATTAACCGATGATTCCGTTATTCCAGTGCCGCTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCACGTCGGTAt                                                                                                                    <  2:698250/140‑1 (MQ=255)                          aTTAGCTTCTGGAATTAACCGATGATTCCGTTATTCCAGTGCCGCTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCACGTCGGTATTTGCt                                                                                                               <  2:680721/140‑1 (MQ=255)                           ttAGCTTCTGGAATTAACCGATGATTCCGTTATTCCAGTGCCGCTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCACGTCGGTATTTGCtt                                                                                                              <  1:651050/140‑1 (MQ=255)                           ttAGCTTCTGGAATTAACCGATGATTCCGTTATTCCAGTGCCGCTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCACGTCGGTATTTGCtt                                                                                                              <  1:1461853/140‑1 (MQ=255)                             aGCTTCTGGAATTAACCGATGATTCCGTTATTCCAGTGCCGCTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCACGTCGGTATTTGCTTcc                                                                                                            <  2:535120/140‑1 (MQ=255)                                  cTGGAATTAACCGATGATTCCGTTATTCCAGTGCCGCTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCACGTCGGTATTTGCTTCcctcct                                                                                                       >  2:235988/1‑140 (MQ=255)                                  cTGGAATTAACCGATGATTCCGTTATTCCAGTGCCGCTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCACGTCGGTATTTGCTTCcctcct                                                                                                       <  1:1462/140‑1 (MQ=255)                                  cTGGAATTAACCGATGATTCCGTTATTCCAGTGCCGCTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCACGTCGGTATTTGCTTCcctcct                                                                                                       >  1:934519/1‑140 (MQ=255)                                   tGGAATTAACCGATGATTCCGTTATTCCAGTGCCGCTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCACGTCGGTATTTGCTTCCCTCCtt                                                                                                      >  2:969479/1‑140 (MQ=255)                                   tGGAATTAACCGATGATTCCGTTATTCCAGTGCCGCTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCACGTCGGTATTTGCTTCCCTCCtt                                                                                                      <  2:313239/140‑1 (MQ=255)                                    ggAATTAACCGATGATTCCGTTATTCCAGTGCCGCTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCCCCTTTCTTAATTATTGTGATATCACGTCGGTATTTGCTTCCCTCCttt                                                                                                     <  2:1019853/140‑1 (MQ=255)                                    ggAATTAACCGATGATTCCGTTATTCCAGTGCCGCTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCACGTCGGTATTTGCTTCCCTCCttt                                                                                                     <  1:969479/140‑1 (MQ=255)                                     gAATTAACCGATGATTCCGTTATTCCAGTGCCGCTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTc                                                                                                    >  2:367158/1‑140 (MQ=255)                                          aaCCGATGATTCCGTTATTCCAGTGCCGCTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAGTCTCAGCCTGAGTAACTCCACCTTTCTTAATTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCt                                                                                               >  2:1672843/1‑140 (MQ=255)                                                tgatTCCGTTATTCCAGTGCCGCTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAAtttt                                                                                         >  1:1414524/1‑140 (MQ=255)                                                    tCCGTTATTCCAGTGCCGCTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCt                                                                                     <  2:40208/140‑1 (MQ=255)                                                     ccGTTATTCCAGTGCCGCTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCtt                                                                                    <  1:795027/140‑1 (MQ=255)                                                           ttCCAGTGCCGCTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAACATg                                                                              <  1:894861/140‑1 (MQ=255)                                                            tCCAGTGCCGCTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAACATGt                                                                             <  1:1728763/140‑1 (MQ=255)                                                                gTGCCGCTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAAttttt                                                                                        >  2:1262227/1‑125 (MQ=255)                                                                gTGCCGCTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAAttttt                                                                                        <  1:1262227/125‑1 (MQ=255)                                                                gTGCCGCTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAACATGt                                                                             <  2:1192245/136‑1 (MQ=255)                                                                gTGCCGCTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAACATGt                                                                             >  1:1192245/1‑136 (MQ=255)                                                                gTGCCGCTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAACATGTCGat                                                                         >  1:1690143/1‑140 (MQ=255)                                                                  gCCGCTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAACATGTCGatat                                                                       <  2:605828/140‑1 (MQ=255)                                                                  gCCGCTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAACATGTCGatat                                                                       <  2:75109/140‑1 (MQ=255)                                                                  gCCGCTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAACATGTCGatat                                                                       >  2:1534924/1‑140 (MQ=255)                                                                    cGCTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAACATGTCGATATCg                                                                     <  1:922215/140‑1 (MQ=255)                                                                      cTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAACATGTCGATATCGGc                                                                   >  2:561839/1‑140 (MQ=255)                                                                             gTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAACATGTCGATATCGGCTTTTAGt                                                            <  2:1306148/140‑1 (MQ=255)                                                                              tCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAACATGTCGATATCGGCTTTTAGtt                                                           >  2:699341/1‑140 (MQ=255)                                                                              tCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAACATGTCGATATCGGCTTTTAGt                                                            <  1:1542718/139‑1 (MQ=255)                                                                              tCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAACATGTCGATATCGGCTTTTAGt                                                            >  2:1542718/1‑139 (MQ=255)                                                                                   aaaCTGATACACAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAACATGTCGATATCGGCTTTTAGTTGAttt                                                      >  1:220579/1‑140 (MQ=255)                                                                                     aCTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAACATGTCGATATCGGCTTTTAGTTGATTTCa                                                    <  1:1534924/140‑1 (MQ=255)                                                                                       tGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAACATGTCGATATCGGCTTTTAGTTGATTTCAGa                                                  <  2:1449573/140‑1 (MQ=255)                                                                                       tGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAACATGTCGATATCGGCTTTTAGTTGATTTCAGa                                                  <  2:812536/140‑1 (MQ=255)                                                                                       tGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAACATGTCGATATCGGCTTTTAGTTGATTTCAGa                                                  <  2:1414524/140‑1 (MQ=255)                                                                                         aTACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAACATGTCGATATCGGCTTTTAGTTGAt                                                        <  2:758401/132‑1 (MQ=255)                                                                                         aTACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAACATGTCGATATCGGCTTTTAGTTGAt                                                        >  1:758401/1‑132 (MQ=255)                                                                                          tACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAACATGTCGATATCGGCTTTTAGTTGATTTCAGATtg                                               >  2:66459/1‑140 (MQ=255)                                                                                          tACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAACATGTCGATATCGGCTTTTAGTTGATTTCAGATtg                                               >  2:1268873/1‑140 (MQ=255)                                                                                               aaTTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAACATGTCGATATCGGCTTTTAGTTGATTTCAGATtgtg                                             >  2:1460085/1‑137 (MQ=255)                                                                                               aaTTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAACATGTCGATATCGGCTTTTAGTTGATTTCAGATtgtg                                             <  1:1460085/137‑1 (MQ=255)                                                                                               aaTTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAACATGTCGATATCGGCTTTTAGTTGATTTCAGATTGTGAAt                                          <  1:319905/140‑1 (MQ=255)                                                                                               aaTTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAACATGTCGATATCGGCTTTTAGTTGATTTCAGATTGTGAAt                                          >  2:1314024/1‑140 (MQ=255)                                                                                               aaTTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAACATGTCGATATCGGCTTTTAGTTGATTTCAGATTGTGAAt                                          >  2:503936/1‑140 (MQ=255)                                                                                                 tttCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAACATGTCGATATCGGCTTTTAGTTGATTTCAGATTGTGAATGt                                        <  1:235988/140‑1 (MQ=255)                                                                                                 tttCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAACATGTCGATATCGGCTTTTAGTTGATTTCAGATTGTGAATGt                                        <  1:253557/140‑1 (MQ=255)                                                                                                 tttCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAACATGTCGATATCGGCTTTTAGTTGATTTCAGATTGTGAATGt                                        <  2:1690143/140‑1 (MQ=255)                                                                                                    cTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAACATGTCGATATCGGCTTTTAGTTGATTTCAGATTGTGAATGTCaa                                     <  2:220579/140‑1 (MQ=255)                                                                                                    cTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAACATGTCGATATCGGCTTTTAGTTGATTTCAGATTGTGAATGTCaa                                     <  1:1451333/140‑1 (MQ=255)                                                                                                    cTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAACATGTCGATATCGGCTTTTAGTTGATTTCAGATTGTGAATGTCaa                                     <  2:565781/140‑1 (MQ=255)                                                                                                    cTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAACATGTCGATATCGGCTTTTAGTTGATTTCAGATTGTGAATGTCaa                                     <  1:503936/140‑1 (MQ=255)                                                                                                      ttGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAACATGTCGATATCGGCTTTTAGTTGATTTCAGATTGTGAATGTCAATc                                   <  2:934519/140‑1 (MQ=255)                                                                                                         cAATCTCAGCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAACATGTCGATATCGGCTTTTAGTTGATTTCAGATTGTGAATGTCAATCGTa                                <  1:367158/140‑1 (MQ=255)                                                                                                                 gCCTGAGTAAATCCACCTTTCTTAATTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAACATGTCGATATCGGCTTTTAGTTGATTTCAGATTGTGAATGTCAATCGTATCCTGACg                        >  1:1708559/1‑140 (MQ=255)                                                                                                                          aaTCCACCTTTCTTAATTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAACATGTCGATATCGGCTTTTAGTTGATTTCAGATTGTGAATGTCAATCGTATCCTGACGAGCAATCaa               >  1:454902/1‑140 (MQ=255)                                                                                                                            tCCACCTTTCTTAATTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAACATGTCGATATCGGCTTTTAGTTGATTTCAGATTGTGAATGTCAATCGTATCCTGACGAGCAATCaaaa             >  1:275160/1‑140 (MQ=255)                                                                                                                               aCCTTTCTTAATTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAACATGTCGATATCGGCTTTTAGTTGATTTCAGATTGTGAATGTCAATCGTATCCTGACGAGCAATCAAAAAAg          <  2:275160/140‑1 (MQ=255)                                                                                                                                ccTTTCTTAATTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAACATGTCGATATCGGCTTTTAGTTGATTTCAGATTGTGAATGTCAATCGTATCCTGACGAGCAATCAAAAAAGa         >  1:365094/1‑140 (MQ=255)                                                                                                                                ccTTTCTTAATTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAACATGTCGATATCGGCTTTTAGTTGATTTCAGATTGTGAATGTCAATCGTATCCTGACGAGCAATCAAAAAAGa         >  1:719781/1‑140 (MQ=255)                                                                                                                                ccTTTCTTAATTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAACATGTCGATATCGGCTTTTAGTTGATTTCAGATTGTGAATGTCAATCGTATCCTGACGAGCAATCAAAAAAGa         >  1:669674/1‑140 (MQ=255)                                                                                                                                ccTTTCTTAATTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAACATGTCGATATCGGCTTTTAGTTGATTTCAGATTGTGAATGTCAATCGTATCCTGACGAGCAATCAAAAAAGa         >  1:139690/1‑140 (MQ=255)                                                                                                                                ccTTTCTTAATTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAACATGTCGATATCGGCTTTTAGTTGATTTCAGATTGTGAATGTCAATCGTATCCTGACGAGCAATCAAAAAAGa         >  2:1737788/1‑140 (MQ=255)                                                                                                                                ccTTTCTTAATTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAACATGTCGATATCGGCTTTTAGTTGATTTCAGATTGTGAATGTCAATCGTATCCTGACGAGCAATCAAAAAAGa         >  2:1502693/1‑140 (MQ=255)                                                                                                                                  tttCTTAATTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAACATGTCGATATCGGCTTTTAGTTGATTTCAGATTGTGAATGTCAATCGTATCCTGACGAg                      >  1:511439/1‑125 (MQ=255)                                                                                                                                  tttCTTAATTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAACATGTCGATATCGGCTTTTAGTTGATTTCAGATTGTGAATGTCAATCGTATCCTGACGAg                      <  2:511439/125‑1 (MQ=255)                                                                                                                                  tttCTTAATTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAACATGTCGATATCGGCTTTTAGTTGATTTCAGATTGTGAATGTCAATCGTATCCTGACGAGCAATCAAAAAAGACa       >  2:773598/1‑140 (MQ=255)                                                                                                                                    tCTTAATTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAACATGTCGATATCGGCTTTTAGTTGATTTCAGATTGTGAATGTCAATCGTATCCTGACGAGCAATCAAAAAAGACAAg     <  1:1737788/140‑1 (MQ=255)                                                                                                                                       tAATTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAACATGTCGATATCGGCTTTTAGTTGATTTCAGATTGTGAATGTCAATCGTATCCTGACGAGCAATCAAAAAAGACAAGCaa  <  2:531739/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         ATGGCTGTTAAAAAGTTACTCTGCAATTAGCTTCTGGAATTAACCGATGATTCCGTTATTCCAGTGCCGCTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAAGTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAACATGTCGATATCGGCTTTTAGTTGATTTCAGATTGTGAATGTCAATCGTATCCTGACGAGCAATCAAAAAAGACAAGCAA  >  NC_000913/293181‑293454

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑