Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	399,371	G→T	intergenic (‑38/‑222)	yaiY ← / → yaiZ	inner membrane protein/DUF2754 domain‑containing protein YaiZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	399,371	0	G	T	100.0%	307.2 / NA	87	intergenic (‑38/‑222)	yaiY/yaiZ	inner membrane protein/DUF2754 domain‑containing protein YaiZ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (34/53);  total (34/53)

AGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAAT  >  NC_000913/399239‑399505                                                                                                                                     |                                                                                                                                       aGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAg                                                                                                                                 <  2:1351755/140‑1 (MQ=255) aGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAg                                                                                                                                 <  1:520511/140‑1 (MQ=255) aGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAg                                                                                                                                 <  1:1586070/140‑1 (MQ=255)   cacaAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCg                                                                                                                               >  2:615325/1‑140 (MQ=255)     caaacaaCGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGc                                                                                                                             >  2:541900/1‑140 (MQ=255)      aaacaaCGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCt                                                                                                                            <  1:905028/140‑1 (MQ=255)        acaacGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTa                                                                                                                          >  1:836107/1‑140 (MQ=255)        acaacGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTa                                                                                                                          >  1:369089/1‑140 (MQ=255)        acaacGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTa                                                                                                                          >  2:16508/1‑140 (MQ=255)          aacGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATg                                                                                                                        <  1:1370900/140‑1 (MQ=255)          aacGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATg                                                                                                                        >  2:394536/1‑140 (MQ=255)             gCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCa                                                                                                                     >  2:1655527/1‑140 (MQ=255)             gCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCa                                                                                                                     <  1:679812/140‑1 (MQ=255)             gCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCa                                                                                                                     >  1:1407102/1‑140 (MQ=255)             gCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCa                                                                                                                     <  2:1431718/140‑1 (MQ=255)             gCATAGGCAATGTTGCCAGGCATTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCa                                                                                                                     >  2:1701507/1‑140 (MQ=255)                tAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGt                                                                                                                  >  1:127354/1‑140 (MQ=255)                tAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGt                                                                                                                  >  1:814873/1‑140 (MQ=255)                tAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGt                                                                                                                  >  1:1422522/1‑140 (MQ=255)                  ggCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGTCt                                                                                                                >  1:1005420/1‑140 (MQ=255)                   gCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGTCTg                                                                                                               <  1:1190653/140‑1 (MQ=255)                      aTGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGTCTGACa                                                                                                            <  2:560180/140‑1 (MQ=255)                       tGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGTCTGACAg                                                                                                           <  1:1012654/140‑1 (MQ=255)                       tGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGTCTGACAg                                                                                                           <  1:619313/140‑1 (MQ=255)                       tGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGTCTGACAg                                                                                                           <  1:541900/140‑1 (MQ=255)                       tGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGTCTGACAg                                                                                                           <  2:1095513/140‑1 (MQ=255)                       tGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGTCTGACAg                                                                                                           <  2:852007/140‑1 (MQ=255)                           gCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGTCTGACAGGTCa                                                                                                       >  1:953212/1‑140 (MQ=255)                            ccAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGTCTGACAGGTCAt                                                                                                      >  2:209457/1‑140 (MQ=255)                              aGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGTCTGACAGGTCAttt                                                                                                    <  1:239421/140‑1 (MQ=255)                               ggCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTa                                                                                                   >  2:237024/1‑140 (MQ=255)                                 cGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGa                                                                                                 <  2:1695605/140‑1 (MQ=255)                                 cGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGa                                                                                                 <  2:438290/140‑1 (MQ=255)                                   ttGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGAtt                                                                                               <  2:1038057/140‑1 (MQ=255)                                    tGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTg                                                                                              >  2:1760216/1‑140 (MQ=255)                                     gAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGGCAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGt                                                                                             <  1:1620228/140‑1 (MQ=255)                                     gAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAGCCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGt                                                                                             <  1:394536/140‑1 (MQ=255)                                     gAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGt                                                                                             <  1:1644451/140‑1 (MQ=255)                                      aaGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGTCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTc                                                                                            <  2:198314/140‑1 (MQ=255)                                      aaGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTc                                                                                            <  1:16508/140‑1 (MQ=255)                                       agagGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCa                                                                                           <  2:953212/140‑1 (MQ=255)                                       agagGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCa                                                                                           <  1:1760216/140‑1 (MQ=255)                                        gagGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCaa                                                                                          <  2:768332/140‑1 (MQ=255)                                           gCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCGatat                                                                                       <  2:36506/140‑1 (MQ=255)                                           gCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAatat                                                                                       >  1:902515/1‑140 (MQ=255)                                            cATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAATATc                                                                                      >  1:980755/1‑140 (MQ=255)                                                gCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAATATCAGaa                                                                                  >  1:1252570/1‑140 (MQ=255)                                                gCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAATATCAGaa                                                                                  <  2:902515/140‑1 (MQ=255)                                                gCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAATATCAGaa                                                                                  <  2:814873/140‑1 (MQ=255)                                                gCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAATATCAGaa                                                                                  <  2:1422522/140‑1 (MQ=255)                                                gCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAATATCAGaa                                                                                  <  1:881824/140‑1 (MQ=255)                                                gCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAATATCAGaa                                                                                  <  2:127354/140‑1 (MQ=255)                                                 cGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAATATCAGAAt                                                                                 >  1:680298/1‑140 (MQ=255)                                                   atatTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAATATCAGAATCa                                                                               <  1:615325/140‑1 (MQ=255)                                                          ccGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAATATCAGAATCATGGTaaa                                                                        <  2:1740332/140‑1 (MQ=255)                                                          ccGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAATATCAGAATCATGGTaaa                                                                        <  2:255588/140‑1 (MQ=255)                                                           cGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAATATCAGAATCATGGTAAAt                                                                       >  1:1537484/1‑140 (MQ=255)                                                            gCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAATATCAGAATCATGGTAAAtt                                                                      <  1:660200/140‑1 (MQ=255)                                                             cTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAATATCAGAATCATGGTAAATTg                                                                     <  2:680298/140‑1 (MQ=255)                                                             cTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAATATCAGAATCATGGTAAATTg                                                                     >  2:1618406/1‑140 (MQ=255)                                                             cTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAATATCAGAATCATGGTAAATTg                                                                     >  2:482506/1‑140 (MQ=255)                                                                     cGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAATATCAGAATCATGGTAAATTGATGTTggg                                                             <  2:596378/140‑1 (MQ=255)                                                                     cGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAATATCAGAATCATGGTAAATTGATGTTggg                                                             >  2:511640/1‑140 (MQ=255)                                                                       aTTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGaa                                                           <  2:1425944/140‑1 (MQ=255)                                                                       aTTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGaa                                                           <  1:1655527/140‑1 (MQ=255)                                                                         tttGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAata                                                         <  2:1005420/140‑1 (MQ=255)                                                                            gACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTc                                                      >  2:107523/1‑140 (MQ=255)                                                                               aGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATTCCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCg                                                   <  2:712353/140‑1 (MQ=255)                                                                                gggTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGa                                                  >  2:1161984/1‑140 (MQ=255)                                                                                  gTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAg                                                >  1:385808/1‑140 (MQ=255)                                                                                    gAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCg                                              <  2:385808/140‑1 (MQ=255)                                                                                    gAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCg                                              <  1:1618406/140‑1 (MQ=255)                                                                                      aaTCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTg                                            <  1:600283/140‑1 (MQ=255)                                                                                           gCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCt                                       >  2:1437104/1‑140 (MQ=255)                                                                                                  aaaCCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTtgt                                <  1:1437104/140‑1 (MQ=255)                                                                                                       tCCGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTc                           >  2:700773/1‑140 (MQ=255)                                                                                                         cGGAAGTCATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTcac                         >  2:623652/1‑140 (MQ=255)                                                                                                                cATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATgg                  <  2:1252570/140‑1 (MQ=255)                                                                                                                cATCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATgg                  <  1:511640/140‑1 (MQ=255)                                                                                                                 aTCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGc                 <  2:836107/140‑1 (MQ=255)                                                                                                                 aTCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGc                 <  2:369089/140‑1 (MQ=255)                                                                                                                  tCTGCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCa                <  1:1701507/140‑1 (MQ=255)                                                                                                                     gCTTCTCTTCTGGTGTTATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAAcc             <  1:482506/140‑1 (MQ=255)                                                                                                                       ttctcttctGGTGTTATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCtt           <  1:725471/140‑1 (MQ=255)                                                                                                                              ctGGTGTTATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTaa    >  2:123845/1‑140 (MQ=255)                                                                                                                                ggTGTTATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAAt  >  1:1534499/1‑140 (MQ=255)                                                                                                                                ggTGTTATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAAt  >  2:298338/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                       AGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAAT  >  NC_000913/399239‑399505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑