Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,893,777	C→A	intergenic (+66/‑356)	yoaB → / → yoaC	RutC family protein YoaB/DUF1889 domain‑containing protein YoaC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,893,777	0	C	A	100.0%	177.9 / NA	50	intergenic (+66/‑356)	yoaB/yoaC	RutC family protein YoaB/DUF1889 domain‑containing protein YoaC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (16/34);  total (16/34)

GCTGCACGGTACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCGAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATCGACCAGAGAATAAGATATTACTGTTGATGCTTCTGCTAATTCATTATTTATATTTATAATTTCAATTTTATCTATAAAACTAACTATATAAATAAACATAAACATGCCGTTGTGTTCGCCCTTTGATTAGCAA  >  NC_000913/1893641‑1893910                                                                                                                                         |                                                                                                                                      gcTGCACGGTACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCGAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATAGAc                                                                                                                                    <  1:1284430/140‑1 (MQ=255) gcTGCACGGTACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCGAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATAGAc                                                                                                                                    <  1:1437827/140‑1 (MQ=255) gcTGCACGGTACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCGAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATAGAc                                                                                                                                    <  2:134900/140‑1 (MQ=255)  cTGCACGGTACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCGAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATAGAcc                                                                                                                                   >  1:805012/1‑140 (MQ=255)   tGCACGGTACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCGAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATAGACCa                                                                                                                                  <  2:269072/140‑1 (MQ=255)   tGCACGGTACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCGAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATAGACCa                                                                                                                                  <  2:565313/140‑1 (MQ=255)     cACGGTACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCGAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATAGACCaga                                                                                                                                <  2:1376959/140‑1 (MQ=255)        ggTACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTGAGCTTTATCGAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATAGACCAGAGaa                                                                                                                             <  1:495923/140‑1 (MQ=255)        ggTACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCGAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATAGACCAGAGaa                                                                                                                             <  2:1527345/140‑1 (MQ=255)        ggTACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCGAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATAGACCAGAGaa                                                                                                                             <  2:281593/140‑1 (MQ=255)        ggTACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCGAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATAGACCAGAGaa                                                                                                                             <  1:1471565/140‑1 (MQ=255)        ggTACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCGAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATAGACCAGAGaa                                                                                                                             <  2:142892/140‑1 (MQ=255)        ggTACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCGAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATAGACCAGAGaa                                                                                                                             <  1:772088/140‑1 (MQ=255)             aaGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCGAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATAGACCAGAGAATAAGa                                                                                                                        <  1:1695542/140‑1 (MQ=255)               gCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCGAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATAGACCAGAGaa                                                                                                                             <  2:1580430/133‑1 (MQ=255)               gCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCGAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATAGACCAGAGaa                                                                                                                             >  1:1580430/1‑133 (MQ=255)                  ggTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCGAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATAGACCAGAGAATAAGATATTa                                                                                                                   <  2:594249/140‑1 (MQ=255)                  ggTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCGAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATAGACCAGAGAATAAGATATTa                                                                                                                   <  1:14122/140‑1 (MQ=255)                    tttGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCGAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATAGACCAGAGAATAAGATATTACt                                                                                                                 >  1:1698406/1‑140 (MQ=255)                    tttGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCGAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATAGACCAGAGAATAAGATATTACt                                                                                                                 <  2:1565675/140‑1 (MQ=255)                    tttGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCGAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATAGACCAGAGAATAAGATATTACt                                                                                                                 >  1:961897/1‑140 (MQ=255)                         tgaACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCGAAACAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATAGACCAGAGAATAAGATATTACTGTTGa                                                                                                            <  1:252495/140‑1 (MQ=255)                             cccGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCGAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATAGACCAGAGAATAAGATATTACTGTTGATGCt                                                                                                        <  1:33114/140‑1 (MQ=255)                             cccGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCGAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATAGACCAGAGAATAAGATATTACTGTTGATGCt                                                                                                        <  2:48299/140‑1 (MQ=255)                             cccGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCGAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATAGACCAGAGAATAAGATATTACTGTTGATGCt                                                                                                        >  2:1111405/1‑140 (MQ=255)                                       aaaGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCGAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATAGACCAGAGAATAAGATATTACTGTTGATGCTTCTGCTAAtt                                                                                              <  1:1254524/140‑1 (MQ=255)                                        aaGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCGAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATAGACCAGAGAATAAGATATTACTGTTGATGCTTCTGCTAATTc                                                                                             <  2:961897/140‑1 (MQ=255)                                              aaaTTAAGATTGTGGCTGCGGTGTAAGCTTTATCGAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATAGACCAGAGAATAAGATATTACTGTTGATGCt                                                                                                        <  1:1176063/123‑1 (MQ=255)                                              aaaTTAAGATTGTGGCTGCGGTGTAAGCTTTATCGAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATAGACCAGAGAATAAGATATTACTGTTGATGCt                                                                                                        >  2:1176063/1‑123 (MQ=255)                                              aaaTTAAGATTGTGGCTGCGGTGTAAGCTTTATCGAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATAGACCAGAGAATAAGATATTACTGTTGATGCTTCTGCTAATTCattatt                                                                                       >  1:463889/1‑140 (MQ=255)                                                                 ggTGTAAGCTTTATCGAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATAGACCAGAGAATAAGATATTACTGTTGATGCTTCTGCTAATTCATTATTTATATTTATAATTTCAAtt                                                                    <  2:802031/140‑1 (MQ=255)                                                                    gtAAGCTTTATCGAAGCAAAATAAGTAAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATAGACCAGAGAATAAGATATTACTGTTGATGCTTCTGCTAATTCATTATTTATATTTATAATTTCAATTTTa                                                                 >  2:408036/1‑140 (MQ=255)                                                                         cTTTATCGAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATAGACCAGAGAATAAGATATTACTGTTGATGCTTCTGCTAATTCATTATTTATATTTATAATTTCAATTTTATCtat                                                            <  1:1509451/140‑1 (MQ=255)                                                                             aTCGAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATAGACCAGAGAATAAGATATTACTGTTGATGCTTCTGCTAATTCATTATTTATATTTATAATTTCAATTTTATCta                                                             <  1:1249904/135‑1 (MQ=255)                                                                             aTCGAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATAGACCAGAGAATAAGATATTACTGTTGATGCTTCTGCTAATTCATTATTTATATTTATAATTTCAATTTTATCta                                                             >  2:1249904/1‑135 (MQ=255)                                                                               cGAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATAGACCAGAGAATAAGATATTACTGTTGATGCTTCTGCTAATTCATTATTTATATTTATAATTTCAATTTTATCTATAAaact                                                      <  1:552797/140‑1 (MQ=255)                                                                                 aaGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATAGACCAGAGAATAAGATATTACTGTTGATGCTTCTGCTAATTCATTATTTATATTTATAATTTCAATTTTATCTATAAaactaa                                                    <  2:463889/140‑1 (MQ=255)                                                                                  aGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATAGACCAGAGAATAAGATATTACTGTTGATGCTTCTGCTAATTCATTATTTATATTTATAATTTCAATTTTATCTATAAaactaac                                                   <  2:805012/140‑1 (MQ=255)                                                                                       aaTAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATAGACCAGAGAATAAGATATTACTGTTGATGCTTCTGCTAATTCATTATTTATATTTATAATTTCAATTTTATCTATAAAACTAACtatat                                              >  2:1551329/1‑140 (MQ=255)                                                                                          aaGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATAGACCAGAGAATAAGATATTACTGTTGATGCTTCTGCTAATTCATTATTTATATTTATAATTTCAATTTTATCTATAAAACTAACTATataaa                                           <  2:1698406/140‑1 (MQ=255)                                                                                          aaGTAAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATAGACCAGAGAATAAGATATTACTGTTGATGCTTCTGCTAATTCATTATTTATATTTATAATTTCAATTTTATCTATAAAACTAACTATataaa                                           <  1:408036/140‑1 (MQ=255)                                                                                                 aCGATAATTTATCGATAATACTGGTCGGTTTTACATAAATAGACCAGAGAATAAGATATTACTGTTGATGCTTCTGCTAATTCATTATTTATATTTATAATTTCAATTTTATCTATAAAACTAACTATATAAataaacat                                    >  1:976924/1‑140 (MQ=255)                                                                                                 aCGATAATTTATCGATAATACTGGTCGGTTTTACATAAATAGACCAGAGAATAAGATATTACTGTTGATGCTTCTGCTAATTCATTATTTATATTTATAATTTCAATTTTATCTATAAAACTAACTATATAAataaacat                                    >  2:541366/1‑140 (MQ=255)                                                                                                           aTCGATAATACTGGTCGGTTTTACATAAATAGACCAGAGAATAAGATATTACTGTTGATGCTTCTGCTAATTCATTATTTATATTTATAATTTCAATTTTATCTATAAAACTAACTATATAAATAAACATAAACATGCCg                          >  2:1500837/1‑140 (MQ=255)                                                                                                             cGATAATACTGGTCGGTTTTACATAAATAGACCAGAGAATAAGATATTACTGTTGATGCTTCTGCTAATTCATTATTTATATTTATAATTTCAATTTTATCTATAAAACTAACTATATAAATAAACATAAACATGCCGtt                        >  2:1151647/1‑140 (MQ=255)                                                                                                                   taCTGGTCGGTTTTACATAAATAGACCAGAGAATAAGATATTACTGTTGATGCTTCTGCTAATTCATTATTTATATTTATAATTTCAATTTTATCTATAAAACTAACTATATAAATAAACATAAACATGCCGTTGTGTTc                  <  1:541366/140‑1 (MQ=255)                                                                                                                        gTCGGTTTTACATAAATAGACCAGAGAATAAGATATTACTGTTGATGCTTCTGCTAATTCATTATTTATATTTATAATTTCAATTTTATCTATAAAACTAACTATATAAATAAACATAAACATGCCGTTGTGTTCGCCCt             >  2:1025421/1‑140 (MQ=255)                                                                                                                                tACATAAATAGACCAGAGAATAAGATATTACTGTTGATGCTTCTGCTAATTCATTATTTATATTTATAATTTCAATTTTATCTATAAAACTAACTATATAAATAAACATAAACATGCCGTTGTGTTCGCCCTTTGATTAg     >  1:1739898/1‑140 (MQ=255)                                                                                                                                 aCATAAATAGACCAGAGAATAAGATATTACTGTTGATGCTTCTGCTAATTCATTATTTATATTTATAATTTCAATTTTATCTATAAAACTAACTATATAAATAAACATAAACATGCCGTTGTGTTCGCCCTTTGATTAGc    <  1:177283/140‑1 (MQ=255)                                                                                                                                   aTAAATAGACCAGAGAATAAGATATTACTGTTGATGCTTCTGCTAATTCATTATTTATATTTATAATTTCAATTTTATCTATAAAACTAACTATATAAATAAACATAAACATGCCGTTGTGTTCGCCCTTTGATTAGCaa  <  1:1500837/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                      GCTGCACGGTACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCGAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATCGACCAGAGAATAAGATATTACTGTTGATGCTTCTGCTAATTCATTATTTATATTTATAATTTCAATTTTATCTATAAAACTAACTATATAAATAAACATAAACATGCCGTTGTGTTCGCCCTTTGATTAGCAA  >  NC_000913/1893641‑1893910

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑