Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	363,940	G→T	P789Q (CCG→CAG)	lacZ ←	beta‑galactosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	363,940	0	G	T	100.0%	234.5 / NA	66	P789Q (CCG→CAG)	lacZ	beta‑galactosidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (32/34);  total (32/34)

CGCATCAGCAAGTGTATCTGCCGTGCACTGCAACAACGCTGCTTCGGCCTGGTAATGGCCCGCCGCCTTCCAGCGTTCGACCCAGGCGTTAGGGTCAATGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTG‑CCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGC  >  NC_000913/363804‑364075                                                                                                                                         |                                                                                                                                         cGCATCAGCAAGTGTATCTGCCGTGCACTGCAACAACGCTGCTTCGGCCTGGTAATGGCCCGCCGCCTTCCAGCGTTCGACCCAGGCGTTAGGGTCAATGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCtgtg                                                                                                                                       <  2:1357492/140‑1 (MQ=255) cGCATCAGCAAGTGTATCTGCCGTGCACTGCAACAACGCTGCTTCGGCCTGGTAATGGCCCGCCGCCTTCCAGCGTTCGACCCAGGCGTTAGGGTCAATGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCtgtg                                                                                                                                       <  2:93183/140‑1 (MQ=255) cGCATCAGCAAGTGTATCTGCCGTGCACTGCAACAACGCTGCTTCGGCCTGGTAATGGCCCGCCGCCTTCCAGCGTTCGACCCAGGCGTTAGGGTCAATGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCtgtg                                                                                                                                       <  1:356090/140‑1 (MQ=255) cGCATCAGCAAGTGTATCTGCCGTGCACTGCAACAACGCTGCTTCGGCCTGGTAATGGCCCGCCGCCTTCCAGCGTTCGACCCAGGCGTTAGGGTCAATGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCtgtg                                                                                                                                       <  2:296085/140‑1 (MQ=255) cGCATCAGCAAGTGTATCTGCCGTGCACTGCAACAACGCTGCTTCGGCCTGGTAATGGCCCGCCGCCTTCCAGCGTTCGACCCAGGCGTTAGGGTCAATGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCtgtg                                                                                                                                       <  1:471134/140‑1 (MQ=255)  gCATCAGCAAGTGTATCTGCCGTGCACTGCAACAACGCTGCTTCGGCCTGGTAATGGCCCGCCGCCTTCCAGCGTTCGACCCAGGCGTTAGGGTCAATGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCTGTGc                                                                                                                                      >  1:268921/1‑140 (MQ=255)   cATCAGCAAGTGTATCTGCCGTGCACTGCAACAACGCTGCTTCGGCCTGGTAATGGCCCGCCGCCTTCCAGCGTTCGACCCAGGCGTTAGGGTCAATGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCTGTGCa                                                                                                                                     <  2:1126894/140‑1 (MQ=255)   cATCAGCAAGTGTATCTGCCGTGCACTGCAACAACGCTGCTTCGGCCTGGTAATGGCCCGCCGCCTTCCAGCGTTCGACCCAGGCGTTAGGGTCAATGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCTGTGCa                                                                                                                                     >  2:866053/1‑140 (MQ=255)   cATCAGCAAGTGTATCTGCCGTGCACTGCAACAACGCTGCTTCGGCCTGGTAATGGCCCGCCGCCTTCCAGCGTTCGACCCAGGCGTTAGGGTCAATGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCTGTGCa                                                                                                                                     <  1:430988/140‑1 (MQ=255)   cATCAGCAAGTGTATCTGCCGTGCACTGCAACAACGCTGCTTCGGCCTGGTAATGGCCCGCCGCCTTCCAGCGTTCGACCCAGGCGTTAGGGTCAATGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCTGTGCa                                                                                                                                     >  2:1737617/1‑140 (MQ=255)   cATCAGCAAGTGTATCTGCCGTGCACTGCAACAACGCTGCTTCGGCCTGGTAATGGCCCGCCGCCTTCCAGCGTTCGACCCAGGCGTTAGGGTCAATGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCTGTGCa                                                                                                                                     <  2:1027881/140‑1 (MQ=255)   cATCAACAAGTGTATCTGCCGTGCACTGCAACAACGCTGCTTCGGCCTGGTAATGGCCCGCCGCCTTCCAGCGTTCGACCCAGGCGTTAGGGTCAATGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCTGTGCa                                                                                                                                     <  1:700978/140‑1 (MQ=255)      cAGCAAGTGTATCTGCCGTGCACTGCAACAACGCTGCTTCGGCCTGGTAATGGCCCGCCGCCTTCCAGCGTTCGACCCAGGCGTTAGGGTCAATGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCTGTGCACgg                                                                                                                                  <  2:762383/140‑1 (MQ=255)        gCAAGTGTATCTGCCGTGCACTGCAACAACGCTGCTTCGGCCTGGTAATGGCCCGCCGCCTTCCAGCGTTCGACCCAGGCGTTAGGGTCAATGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCtgtg                                                                                                                                       <  1:1576095/133‑1 (MQ=255)        gCAAGTGTATCTGCCGTGCACTGCAACAACGCTGCTTCGGCCTGGTAATGGCCCGCCGCCTTCCAGCGTTCGACCCAGGCGTTAGGGTCAATGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCtgtg                                                                                                                                       >  2:180483/1‑133 (MQ=255)        gCAAGTGTATCTGCCGTGCACTGCAACAACGCTGCTTCGGCCTGGTAATGGCCCGCCGCCTTCCAGCGTTCGACCCAGGCGTTAGGGTCAATGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCtgtg                                                                                                                                       >  2:1576095/1‑133 (MQ=255)        gCAAGTGTATCTGCCGTGCACTGCAACAACGCTGCTTCGGCCTGGTAATGGCCCGCCGCCTTCCAGCGTTCGACCCAGGCGTTAGGGTCAATGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCtgtg                                                                                                                                       <  1:180483/133‑1 (MQ=255)         cAAGTGTATCTGCCGTGCACTGCAACAACGCTGCTTCGGCCTGGTAATGGCCCGCCGCCTTCCAGCGTTCGACCCAGGCGTTAGGGTCAATGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCTGTGCACGGGTg                                                                                                                               <  1:1289944/140‑1 (MQ=255)           aGTGTATCTGCCGTGCACTGCAACAACGCTGCTTCGGCCTGGTAATGGCCCGCCGCCTTCCAGCGTTCGACCCAGGCGTTAGGGTCAATGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCTGTGCACGGGTGaa                                                                                                                             >  1:1058664/1‑140 (MQ=255)             tgtATCTGCCGTGCACTGCAACAACGCTGCTTCGGCCTGGTAATGGCCCGCCGCCTTCCAGCGTTCGACCCAGGCGTTAGGGTCAATGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCTGTGCACGGGTGAACt                                                                                                                           <  1:1207021/140‑1 (MQ=255)             tgtATCTGCCGTGCACTGCAACAACGCTGCTTCGGCCTGGTAATGGCCCGCCGCCTTCCAGCGTTCGACCCAGGCGTTAGGGTCAATGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCTGTGCACGGGTGAACt                                                                                                                           >  2:187032/1‑140 (MQ=255)               tATCTGCCGTGCACTGCAACAACGCTGCTTCGGCCTGGTAATGGCCCGCCGCCTTCCAGCGTTCGACCCAGGCGTTAGGGTCAATGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCTGTGCACGGGTGAACTGa                                                                                                                         <  1:866053/140‑1 (MQ=255)                       gTGCACTGCAACAACGCTGCTTCGGCCTGGTAATGGCCCGCCGCCTTCCAGCGTTCGACCCAGGCGTTAGGGTCAATGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCTGTGCACGGGTGAACTGATCGCGCAg                                                                                                                 >  1:708938/1‑140 (MQ=255)                          cACTGCAACAACGCTGCTTCGGCCTGGTAATGGCCCGCCG‑CTTCCAGCGTTCGACCCAGGCGTTAGGGTCAATGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCTGTGCACGGGTGAACTGATCGCGCAgcggc                                                                                                             <  2:1037879/140‑1 (MQ=255)                           aCTGCAACAACGCTGCTTCGGCCTGGTAATGGCCCGCCGCCTTCCAGCGTTCGACCCAGGCGTTAGGGTCAATGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCTGTGCACGGGTGAACTGATCGCGCAgcggc                                                                                                             <  2:1264899/140‑1 (MQ=255)                               caacaaCGCTGCTTCGGCCTGGTAATGGCCCGCCGCCTTCCAGCGTTCGACCCAGGCGTTAGGGTCAATGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCTGTGCACGGGTGAACTGATCGCGCAGCGGCGTca                                                                                                         >  1:952843/1‑140 (MQ=255)                               caacaaCGCTGCTTCGGCCTGGTAATGGCCCGCCGCCTTCCAGCGTTCGACCCAGGCGTTAGGGTCAATGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCTGTGCACGGGTGAACTGATCGCGCAGCGGCGTca                                                                                                         >  1:320072/1‑140 (MQ=255)                                  caacGCTGCTTCGGCCTGGTAATGGCCCGCCGCCTTCCAGCGTTCGACCCAGGCGTTAGGGTCAATGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCTGTGCACGGGTGAACTGATCGCGCAGCGGCGTcagca                                                                                                      >  2:584768/1‑140 (MQ=255)                                    acGCTGCTTCGGCCTGGTAATGGCCCGCCGCCTTCCAGCGTTCGACCCAGGCGTTAGGGTCAATGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCTGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAgt                                                                                                    >  1:859140/1‑140 (MQ=255)                                     cGCTGCTTCGGCCTGGTAATGGCCCGCCGCCTTCCAGCGTTCGACCCAGGCGTTAGGGTCAATGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCTGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAgtt                                                                                                   >  2:1539263/1‑140 (MQ=255)                                       ctgctTCGGCCTGGTAATGGCCCGCCGCCTTCCAGCGTTCGACCCAGGCGTTAGGGTCAATGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCTGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAgttgt                                                                                                 <  1:584768/140‑1 (MQ=255)                                                 cTGGTAATGGCCCGCCGCCTTCCAGCGTTCGACCCAGGCGTTAGGGTCAATGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCTGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGc                                                                                       <  2:859140/140‑1 (MQ=255)                                                   ggTAATGGCCCGCCGCCTTCCAGCGTTCGACCCAGGCGTTAGGGTCAATGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCTGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCa                                                                                     <  1:276434/140‑1 (MQ=255)                                                    gTAATGGCCCGCCGCCTTCCAGCGTTCGACCCAGGCGTTAGGGTCAATGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCTGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGc                                                                                       >  1:993994/1‑137 (MQ=255)                                                    gTAATGGCCCGCCGCCTTCCAGCGTTCGACCCAGGCGTTAGGGTCAATGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCTGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGc                                                                                       <  2:993994/137‑1 (MQ=255)                                                    gTAATGGCCCGCCGCCTTCCAGCGTTCGACCCAGGCGTTAGGGTCAATGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCTGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGc                                                                                       <  2:1471838/137‑1 (MQ=255)                                                    gTAATGGCCCGCCGCCTTCCAGCGTTCGACCCAGGCGTTAGGGTCAATGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCTGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGc                                                                                       >  1:1471838/1‑137 (MQ=255)                                                    gTAATGGCCCGCCGCCTTCCAGCGTTCGACCCAGGCGTTAGGGTCAATGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCTGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCaa                                                                                    >  1:498775/1‑140 (MQ=255)                                                     tAATGGCCCGCCGCCTTCCAGCGTTCGACCCAGGCGTTAGGGTCAATGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCTGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGtttttt                                                                                            >  1:946597/1‑131 (MQ=255)                                                     tAATGGCCCGCCGCCTTCCAGCGTTCGACCCAGGCGTTAGGGTCAATGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCTGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGtttttt                                                                                            <  2:946597/131‑1 (MQ=255)                                                         ggCCCGCCGCCTTCCAGCGTTCGACCCAGGCGTTAGGGTCAATGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCTGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCcac                                                                               <  2:429991/140‑1 (MQ=255)                                                         ggCCCGCCGCCTTCCAGCGTTCGACCCAGGCGTTAGGGTCAATGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCTGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCcac                                                                               <  1:1760028/140‑1 (MQ=255)                                                          gCCCGCCGCCTTCCAGCGTTCGACCCAGGCGTTAGGGTCAATGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCTGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCcaca                                                                              >  2:581170/1‑140 (MQ=255)                                                          gCCCACCGCCTTCCAGCGTTCGACCCAGGCGTTAGGGTCAATGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCTGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCcaca                                                                              >  2:628263/1‑140 (MQ=255)                                                           cccGCCGCCTTCCAGCGTTCGACCCAGGCGTTAGGGTCAATGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCTGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACAt                                                                             <  1:1656504/140‑1 (MQ=255)                                                                    ttCCAGCGTTCGACCCAGGCGTTAGGGTCAATGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCTGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTgaaag                                                                    <  2:498775/140‑1 (MQ=255)                                                                     tCCAGCGTTCGACCCAGGCGTTAGGGTCAATGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCTGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTgaaaga                                                                   <  1:131136/140‑1 (MQ=255)                                                                      ccAGCGTTCGACCCAGGCGTTAGGGTCAATGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCTGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTgaaagaa                                                                  <  1:187032/140‑1 (MQ=255)                                                                             tCGACCCAGGCGTTAGGGTCAATGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCTGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGa                                                           >  1:1192437/1‑140 (MQ=255)                                                                                   cAGGCGTTAGGGTCAATGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCTGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCg                                                     <  2:320072/140‑1 (MQ=255)                                                                                    aGGCGTTAGGGTCAATGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCTGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCgg                                                    >  2:156647/1‑140 (MQ=255)                                                                                     ggCGTTAGGGTCAATGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCTGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGt                                                   >  1:1428154/1‑140 (MQ=255)                                                                                      gCGTTAGGGTCAATGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCTGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGtt                                                  >  2:1133164/1‑140 (MQ=255)                                                                                           aGGGTCAATGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCTGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTaaaat                                             >  1:695603/1‑138 (MQ=255)                                                                                           aGGGTCAATGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCTGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAAtt                                             >  2:33481/1‑140 (MQ=255)                                                                                              gTCAATGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCTGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTG‑cc                                         <  1:263410/140‑1 (MQ=255)                                                                                               tCAATGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCTGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTG‑CCa                                        <  2:1058664/140‑1 (MQ=255)                                                                                                cAATGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCTGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTG‑CCaa                                       <  1:33481/140‑1 (MQ=255)                                                                                                cAATGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCTGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTG‑CCaa                                       <  1:1211257/140‑1 (MQ=255)                                                                                                 aaTGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCTGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTG‑CCAAc                                      <  2:708938/140‑1 (MQ=255)                                                                                                 aaTGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCTGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTG‑CCAAc                                      <  1:1737617/140‑1 (MQ=255)                                                                                                   tGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCTGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTG‑CCAAc                                      >  2:546134/1‑138 (MQ=255)                                                                                                   tGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCTGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTG‑CCAAc                                      <  1:546134/138‑1 (MQ=255)                                                                                                      gggTCGCTTCACTTACGCCAATGTCGTTATCCAGCTGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTG‑CCAACGCtta                                 <  2:469827/140‑1 (MQ=255)                                                                                                      gggTCGCTTCACTTACGCCAATGTCGTTATCCAGCTGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTG‑CCAACGCtta                                 <  2:952843/140‑1 (MQ=255)                                                                                                             ttCACTTACGCCAATGTCGTTATCCAGCTGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTTAATTT‑CCAACGCTTATTAaccc                          >  1:1740469/1‑139 (MQ=255)                                                                                                                          aTGTCGTTATCCAGCTGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTTAATTT‑CCCACCCTTATTACCCAGCCCGATGCaaaa             >  1:695739/1‑140 (MQ=255)                                                                                                                          aTGTCGTTATCCAGCTGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTG‑CCAACGCTTATTACCCCGCTCGATGCaaaa             >  1:1414668/1‑140 (MQ=255)                                                                                                                          aTGTCGTTATCCAGCTGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTG‑CCAACGCTTATTACCCACCTCGATGCaaaa             >  1:319787/1‑140 (MQ=255)                                                                                                                          aTGTCGTTATCCAGCTGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTG‑CCAACGCCTATTAACCACCCCGGTGCaaaa             >  2:1630650/1‑140 (MQ=255)                                                                                                                           tGTCGTTATCCAGCTGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTG‑CCCACGCTTTTTTCCCAGCTCGCTGCcaaaa            >  1:1043515/1‑140 (MQ=255)                                                                                                                                ttATCCAGCTGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAAATG‑CCAACGCCTATTACCCCGCCCGATGCaaaaaaacat       >  2:817087/1‑135 (MQ=255)                                                                                                                                  aTCCAGCTGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTG‑CCAACGCTTATTACCCAGCTCGATGCAAAAATCCAttt     <  2:805269/140‑1 (MQ=255)                                                                                                                                     cAGCTGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCCAACCCTT‑TTACCCCGCCCCATGCAAAAAACCAtttttc  >  2:609311/1‑137 (MQ=255)                                                                                                                                     cAGCTGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTG‑CCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGc  <  2:795967/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         CGCATCAGCAAGTGTATCTGCCGTGCACTGCAACAACGCTGCTTCGGCCTGGTAATGGCCCGCCGCCTTCCAGCGTTCGACCCAGGCGTTAGGGTCAATGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTG‑CCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGC  >  NC_000913/363804‑364075

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑