Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,083,225	G→T	N866K (AAC→AAA)	fdoG ←	formate dehydrogenase O subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,083,225	0	G	T	100.0%	354.8 / NA	99	N866K (AAC→AAA)	fdoG	formate dehydrogenase O subunit alpha
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (56/43);  total (56/43)

TTCAACAGCGCGTGTTTGGTCCAGTAGTGGAAGTGCTCGGTCAGACGATAGGTGGTTCCGACATACGGGAACTTATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGACGC  >  NC_000913/4083088‑4083358                                                                                                                                          |                                                                                                                                      ttCAACAGCGCGTGTTTGGTCCAGTAGTGGAAGTGCTCGGTCAGACGATAGGTGGTTCCGACATACGGGAACTTATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACttt                                                                                                                                     >  1:592743/1‑140 (MQ=255)     aCAGCGCGTGTTTGGTCCAGTAGTGGAAGTGCTCGGTCAGACGATAGGTGGTTCCGACATACGGGAACTTATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGa                                                                                                                                 <  2:650149/140‑1 (MQ=255)           cgTGTTTGGTCCAGTAGTGGAAGTGCTCGGTCAGACGATAGGTGGTTCCGACATACGGGAACTTATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGc                                                                                                                           >  1:676847/1‑140 (MQ=255)                    tCCAGTAGTGGAAGTGCTCGGTCAGACGATAGGTGGTTCCGACATACGGGAACTTATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGtt                                                                                                                  >  1:886118/1‑140 (MQ=255)                        gtagtGGAAGTGCTCGGTCAGACGATAGGTGGTTCCGACATACGGGAACTTATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCa                                                                                                              >  1:477433/1‑140 (MQ=255)                           gtGGAAGTGCTCGGTCAGACGATAGGTGGTTCCGACATACGGGAACTTATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAgcg                                                                                                           <  2:592743/140‑1 (MQ=255)                           gtGGAAGTGCTCGGTCAGACGATAGGTGGTTCCGACATACGGGAACTTATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAgcg                                                                                                           <  1:1467831/140‑1 (MQ=255)                           gtGGAAGTGCTCGGTCAGACGATAGGTGGTTCCGACATACGGGAACTTATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAgcg                                                                                                           <  2:1685786/140‑1 (MQ=255)                            tGGAAGTGCTCGGTCAGACGATAGGTGGTTCCGACATACGGGAACTTATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAgcgg                                                                                                          <  2:477433/140‑1 (MQ=255)                              gAAGTGCTCGGTCAGACGATAGGTGGTTCCGACATACGGGAACTTATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAgcggcg                                                                                                        >  1:929549/1‑140 (MQ=255)                              gAAGTGCTCGGTCAGACGATAGGTGGTTCCGACATACGGGAACTTATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAgcggcg                                                                                                        >  2:546633/1‑140 (MQ=255)                              gAAGTGCTCGGTCAGACGATAGGTGGTTCCGACATACGGGAACTTATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAgcggcg                                                                                                        >  2:1025479/1‑140 (MQ=255)                                 gTGCTCGGTCAGACGATAGGTGGTTCCGACATACGGGAACTTATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGttt                                                                                                     >  1:1580138/1‑140 (MQ=255)                                  tGCTCGGTCAGACGATAGGTGGTTCCGACATACGGGAACTTATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTc                                                                                                    <  2:929549/140‑1 (MQ=255)                                   gCTCGGTCAGACGATAGGTGGTTCCGACATACGGGAACTTATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCa                                                                                                   >  2:890180/1‑140 (MQ=255)                                   gCTCGGTCAGACGATAGGTGGTTCCGACATACGGGAACTTATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCa                                                                                                   >  2:695359/1‑140 (MQ=255)                                   gCTCGGTCAGACGATAGGTGGTTCCGACATACGGGAACTTATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCa                                                                                                   >  1:275425/1‑140 (MQ=255)                                   gCTCGGTCAGACGATAGGTGGTTCCGACATACGGGAACTTATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCa                                                                                                   >  2:1261122/1‑140 (MQ=255)                                   gCTCGGTCAGACGATAGGTGGTTCCGACATACGGGAACTTATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGAGGCGTTTCa                                                                                                   >  1:1606589/1‑140 (MQ=255)                                    cTCGGTCAGACGATAGGTGGTTCCGACATACGGGAACTTATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCaa                                                                                                  <  2:1655747/140‑1 (MQ=255)                                      cGGTCAGACGATAGGTGGTTCCGACATACGGGAACTTATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAAc                                                                                                <  1:1261122/140‑1 (MQ=255)                                         tCAGACGATAGGTGGTTCCGACATACGGGAACTTATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGc                                                                                             <  2:1756142/140‑1 (MQ=255)                                            gACGATAGGTGGTTCCGACATACGGGAACTTATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCg                                                                                          >  1:1751076/1‑140 (MQ=255)                                             aCGATAGGTGGTTCCGACATACGGGAACTTATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGc                                                                                             <  1:347862/136‑1 (MQ=255)                                             aCGATAGGTGGTTCCGACATACGGGAACTTATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGc                                                                                             >  2:347862/1‑136 (MQ=255)                                              cGATAGGTGGTTCCGACATACGGGAACTTATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTa                                                                                        <  2:1751076/140‑1 (MQ=255)                                                aTAGGTGGTTCCGACATACGGGAACTTATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGAGGCGTTTCAAACGGCTCGTAgt                                                                                      <  2:1606589/140‑1 (MQ=255)                                                  aGGTGGTTCCGACATACGGGAACTTATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAgtgt                                                                                    >  2:1107029/1‑140 (MQ=255)                                                   ggtggtTCCGACATACGGGAACTTATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGtt                                                                                   >  2:1651014/1‑140 (MQ=255)                                                   ggtggtTCCGACATACGGGAACTTATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGtt                                                                                   >  1:1104776/1‑140 (MQ=255)                                                   ggtggtTCCGACATACGGGAACTTATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGtt                                                                                   >  2:612812/1‑140 (MQ=255)                                                   ggtggtTCCGACATACGGGAACTTATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGtt                                                                                   >  2:679257/1‑140 (MQ=255)                                                   ggtggtTCCGACATACGGGAACTTATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGtt                                                                                   >  1:822108/1‑140 (MQ=255)                                                    gtggtTCCGACATACGGGAACTTATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTc                                                                                  <  2:1580138/140‑1 (MQ=255)                                                     tggtTCCGACATACGGGAACTTATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTcc                                                                                 <  1:679257/140‑1 (MQ=255)                                                     tggtTCCGACATACGGGAACTTATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTcc                                                                                 <  1:612812/140‑1 (MQ=255)                                                      ggtTCCGACATACGGGAACTTATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCg                                                                                >  2:1323406/1‑140 (MQ=255)                                                         tCCGACATACGGGAACTTATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGa                                                                             <  1:1323406/140‑1 (MQ=255)                                                             aCATACGGGAACTTATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACgg                                                                         >  1:851491/1‑140 (MQ=255)                                                              cATACGGGAACTTATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGa                                                                        >  1:650544/1‑140 (MQ=255)                                                               aTACGGGAACTTATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGAc                                                                       >  2:104374/1‑140 (MQ=255)                                                                 aCGGGAACTTATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGAc                                                                       >  2:416602/1‑138 (MQ=255)                                                                 aCGGGAACTTATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGAc                                                                       <  1:416602/138‑1 (MQ=255)                                                                 aCGGGAACTTATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCt                                                                     >  2:1408121/1‑140 (MQ=255)                                                                 aCGGGAACTTATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCt                                                                     >  2:734285/1‑140 (MQ=255)                                                                   gggAACTTATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTc                                                                   >  1:645119/1‑140 (MQ=255)                                                                     gAACTTATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCg                                                                 <  1:1597340/140‑1 (MQ=255)                                                                     gAACTTATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCg                                                                 <  1:1408121/140‑1 (MQ=255)                                                                     gAACTTATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCg                                                                 <  2:886118/140‑1 (MQ=255)                                                                     gAACTTATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCg                                                                 <  2:1190222/140‑1 (MQ=255)                                                                       aCTTATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGcc                                                               >  2:1792432/1‑140 (MQ=255)                                                                        cTTATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCa                                                              >  1:311065/1‑140 (MQ=255)                                                                        cTTATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCa                                                              <  2:650544/140‑1 (MQ=255)                                                                           aTCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCt                                                           >  1:1484775/1‑140 (MQ=255)                                                                           aTCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCt                                                           >  1:288727/1‑140 (MQ=255)                                                                           aTCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCt                                                           >  2:238604/1‑140 (MQ=255)                                                                             cGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTa                                                         >  1:1603982/1‑140 (MQ=255)                                                                                cTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCg                                                      <  2:475306/140‑1 (MQ=255)                                                                                   tACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAgg                                                   >  1:406528/1‑140 (MQ=255)                                                                                   tACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAgg                                                   >  2:1368068/1‑140 (MQ=255)                                                                                   tACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAgg                                                   <  2:822108/140‑1 (MQ=255)                                                                                     cccAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGc                                                 <  2:1407609/140‑1 (MQ=255)                                                                                      ccAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCa                                                <  1:1147164/140‑1 (MQ=255)                                                                                         aTGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAAc                                             >  1:1241613/1‑140 (MQ=255)                                                                                          tGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACa                                            >  2:395290/1‑140 (MQ=255)                                                                                          tGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACa                                            >  2:1435911/1‑140 (MQ=255)                                                                                          tGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACa                                            >  2:1463180/1‑140 (MQ=255)                                                                                          tGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACa                                            >  2:1210988/1‑140 (MQ=255)                                                                                          tGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACa                                            >  1:1221639/1‑140 (MQ=255)                                                                                            cTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGa                                          <  1:1107029/140‑1 (MQ=255)                                                                                            cTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGa                                          <  1:890180/140‑1 (MQ=255)                                                                                              tCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACg                                        <  1:1397405/140‑1 (MQ=255)                                                                                               cGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGc                                       >  1:980569/1‑140 (MQ=255)                                                                                               cGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGc                                       >  2:1069488/1‑140 (MQ=255)                                                                                                  cgtcgtCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCTGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGa                                          <  2:1592200/134‑1 (MQ=255)                                                                                                  cgtcgtCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGa                                          >  1:1592200/1‑134 (MQ=255)                                                                                                   gtcgtcTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCa                                   <  1:1435911/140‑1 (MQ=255)                                                                                                   gtcgtcTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCa                                   <  1:1210988/140‑1 (MQ=255)                                                                                                   gtcgtcTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCa                                   <  1:1368068/140‑1 (MQ=255)                                                                                                     cgtcTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATg                                 <  1:1651014/140‑1 (MQ=255)                                                                                                     cgtcTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATg                                 <  1:238604/140‑1 (MQ=255)                                                                                                     cgtcTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATg                                 <  2:1603982/140‑1 (MQ=255)                                                                                                        cTTTAAAGATACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCt                              <  2:288727/140‑1 (MQ=255)                                                                                                                 tACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCctgct                       <  2:1171409/138‑1 (MQ=255)                                                                                                                 tACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCctgct                       >  1:1171409/1‑138 (MQ=255)                                                                                                                 tACGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCtgctgc                     >  1:473136/1‑140 (MQ=255)                                                                                                                  aCGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCa                                   >  1:1111547/1‑125 (MQ=255)                                                                                                                  aCGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCa                                   <  2:1111547/125‑1 (MQ=255)                                                                                                                   cGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCAt                   <  2:645119/140‑1 (MQ=255)                                                                                                                   cGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCAt                   >  1:1201566/1‑140 (MQ=255)                                                                                                                   cGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCAt                   >  1:1608873/1‑140 (MQ=255)                                                                                                                   cGTGCAGCCGGGTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCAt                   >  2:275646/1‑140 (MQ=255)                                                                                                                    gTGCAGCCGGTTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCctg                         <  2:744975/133‑1 (MQ=255)                                                                                                                    gTGCAGCCGGTTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCctg                         >  1:744975/1‑133 (MQ=255)                                                                                                                            gggTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACgg          <  2:473136/140‑1 (MQ=255)                                                                                                                             ggTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGGCCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGc         >  2:204592/1‑140 (MQ=255)                                                                                                                              gTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGcc        >  2:1044159/1‑140 (MQ=255)                                                                                                                              gTTCGAGATAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGcc        >  2:466871/1‑140 (MQ=255)                                                                                                                                    gaTAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGAcgc  >  1:138649/1‑140 (MQ=255)                                                                                                                                    gaTAACTTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGAcgc  <  1:53777/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                      TTCAACAGCGCGTGTTTGGTCCAGTAGTGGAAGTGCTCGGTCAGACGATAGGTGGTTCCGACATACGGGAACTTATCGGCTTTACCCAATGCTTCGGCGTCGTCTTTAAAGATACGTGCAGCCGGGTTCGAGATAACGTTTGGATGCAGCGGGTTAGTTCCCAGCGGCGTTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGACGC  >  NC_000913/4083088‑4083358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑