Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	553,507	G→T	P145Q (CCG→CAG)	lpxH ←	UDP‑2,3‑diacylglucosamine diphosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	553,507	0	G	T	100.0%	237.8 / NA	67	P145Q (CCG→CAG)	lpxH	UDP‑2,3‑diacylglucosamine diphosphatase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (32/35);  total (32/35)

ATTGCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACGGCAGGGCGAGGAATAGCGTCTGCAGCCAGGGTTTGTGGACCTTGGCGCGAAAAGCCTGATAACCCGCGTCATCGGTGCACAGCGTGTCGCCATGCATAATCAACACCCGGCGACCATAAAGTTCGAGCACCTTTT  >  NC_000913/553373‑553642                                                                                                                                       |                                                                                                                                        aTTGCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACTGCAgg                                                                                                                                    >  1:499944/1‑140 (MQ=255) aTTGCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACTGCAgg                                                                                                                                    <  1:855275/140‑1 (MQ=255)  ttGCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACTGCAggg                                                                                                                                   >  1:1070964/1‑140 (MQ=255)   tGCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACTGCAGGGc                                                                                                                                  >  1:1029525/1‑140 (MQ=255)   tGCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACTGCAGGGc                                                                                                                                  >  1:960833/1‑140 (MQ=255)    gCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACTGCAgg                                                                                                                                    <  2:1545781/137‑1 (MQ=255)    gCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACTGCAgg                                                                                                                                    >  1:1545781/1‑137 (MQ=255)           aTGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACTGCAGGGCGAGGAATa                                                                                                                          <  1:512796/140‑1 (MQ=255)                  tCCATCGCACTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACTGCAGGGCGAGGAATAGCGTCTg                                                                                                                   <  1:925002/140‑1 (MQ=255)                    cATCGCACTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACTGCAGGGCGAGGAATAGCGTCTGCa                                                                                                                 <  2:323456/140‑1 (MQ=255)                       cGCACTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACTGCAGGGCGAGGAATAGCGTCTGTAGcc                                                                                                              <  1:1683279/140‑1 (MQ=255)                       cGCACTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACTGCAGGGCGAGGAATAGCGTCTGCAGcc                                                                                                              <  1:1790693/140‑1 (MQ=255)                        gCACTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACTGCAGGGCGAGGAATAGCGTCTGCAGCCa                                                                                                             >  1:1309976/1‑140 (MQ=255)                         cACTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACTGCAGGGCGAGGAATAGCGTCTGCAGCCAg                                                                                                            <  2:125464/140‑1 (MQ=255)                          aCTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACTGCAGGGCGAGGAATAGCGTCTGCAGCCAgg                                                                                                           <  1:1631811/140‑1 (MQ=255)                          aCTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACTGCAGGGCGAGGAATAGCGTCTGCAGCCAgg                                                                                                           <  1:99633/140‑1 (MQ=255)                              accaccGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACTGCAGGGCGAGGAATAGCGTCTGCAGCCAGGGttt                                                                                                       >  2:1501968/1‑140 (MQ=255)                                   cgcgTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACTGCAGGGCGAGGAATAGCGTCTGCAGCCAGGGTTTGTGGa                                                                                                  >  1:593711/1‑140 (MQ=255)                                   cgcgTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACTGCAGGGCGAGGAATAGCGTCTGCAGCCAGGGTTTGTGGa                                                                                                  >  2:880886/1‑140 (MQ=255)                                     cgTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACTGCAGGGCGAGGAATAGCGTCTGCAGCCAGGGTTTGTGGAcc                                                                                                <  1:1501968/140‑1 (MQ=255)                                        tttGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACTGCAGGGCGAGGAATAGCGTCTGCAGCCAGGGTTTGTGGACCTTg                                                                                             >  2:1613663/1‑140 (MQ=255)                                                aCGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACTGCAGGGCGAGGAATAGCGTCTGCAGCCAGGGTTTGTGGACCTTGGCGCGaaa                                                                                     >  1:1030644/1‑140 (MQ=255)                                                      aTGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACTGCAGGGCGAGGAATAGCGTCTGCAGCCAGGGTTTGTGGACCTTGGCGCGAAAAGCCTg                                                                               >  2:1710943/1‑140 (MQ=255)                                                           cGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACTGCAGGGCGAGGAATAGCGTCTGCAGCCAGGGTTTGTGGACCTTGGCGCGAAAAGCCTGATAAc                                                                          >  2:1381456/1‑140 (MQ=255)                                                            gCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACTGCAGGGCGAGGAATAGCGTCTGCAGCCAGGGTTTGTGGACCTTGGCGCGAAAAGCCTGATAAcc                                                                         >  2:971924/1‑140 (MQ=255)                                                            gCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACTGCAGGGCGAGGAATAGCGTCTGCAGCCAGGGTTTGTGGACCTTGGCGCGAAAAGCCTGATAAcc                                                                         >  1:674057/1‑140 (MQ=255)                                                             ccAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACTGCAGGGCGAGGAATAGCGTCTGCAGCCAGGGTTTGTGGACCTTGGCGCGAAAAGCCTGATAAccc                                                                        >  2:308549/1‑140 (MQ=255)                                                             ccAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACTGCAGGGCGAGGAATAGCGTCTGCAGCCAGGGTTTGTGGACCTTGGCGCGAAAAGCCTGATAAccc                                                                        >  2:1769980/1‑140 (MQ=255)                                                              cAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACTGCAGGGCGAGGAATAGCGTCTGCAGCCAGGGTTTGTGGACCTTGGCGCGAAAAGCCTGATAACCcg                                                                       >  1:574077/1‑140 (MQ=255)                                                              cAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACTGCAGGGCGAGGAATAGCGTCTGCAGCCAGGGTTTGTGGACCTTGGCGCGAAAAGCCTGATAACCcg                                                                       >  1:1693465/1‑140 (MQ=255)                                                               aGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACTGCAGGGCGAGGAATAGCGTCTGCAGCCAGGGTTTGTGGACCTTGGCGCGAAAAGCCTGATAACCcgc                                                                      <  2:980390/140‑1 (MQ=255)                                                               aGCGATTTACTGCTGTTGGCTTCTTTGCTGTT‑GCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACTGCAGGGCGAGGAATAGCGTCTGCAGCCAGGGTTTGTGGACCTTGGCGCGAAAAGCCTGATAACCcgcg                                                                     >  2:73387/1‑140 (MQ=255)                                                                 cGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACTGCAGGGCGAGGAATAGCGTCTGCAGCCAGGGTTTGTGGACCTTGGCGCGAAAAGCCTGATAACCCGCGt                                                                    <  2:977548/140‑1 (MQ=255)                                                                 cGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACTGCAGGGCGAGGAATAGCGTCTGCAGCCAGGGTTTGTGGACCTTGGCGCGAAAAGCCTGATAACCCGCGt                                                                    <  2:173917/140‑1 (MQ=255)                                                                   aTTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACTGCAGGGCGAGGAATAGCGTCTGCAGCCAGGGTTTGTGGACCTTGGCGCGAAAAGCCTGATAACCCGCGTCa                                                                  <  1:1710943/140‑1 (MQ=255)                                                                    tttACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACTGCAGGGCGAGGAATAGCGTCTGCAGCCAGGGTTTGTGGACCTTGGCGCGAAAAGCCTGATAACCCGCGTCAt                                                                 <  2:499944/140‑1 (MQ=255)                                                                     ttACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACTGCAGGGCGAGGAATAGCGTCTGCAGCCAGGGTTTGTGGACCTTGGCGCGAAAAGCCTGATAACCCGCGTCATc                                                                <  1:340154/140‑1 (MQ=255)                                                                     ttACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACTGCAGGGCGAGGAATAGCGTCTGCAGCCAGGGTTTGTGGACCTTGGCGCGAAAAGCCTGATAACCCGCGTCATc                                                                <  1:831700/140‑1 (MQ=255)                                                                      tACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACTGCAGGGCGAGGAATAGCGTCTGCAGCCAGGGTTTGTGGACCTTGGCGCGAAAAGCCTGATAACCCGCGTCATCg                                                               <  2:674057/140‑1 (MQ=255)                                                                             gTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACTGCAGGGCGAGGAATAGCGTCTGCAGCCAGGGTTTGTGGACCTTGGCGCGAAAAGCCTGATAACCCGCGTCATCGGTGcaca                                                        <  2:1030644/140‑1 (MQ=255)                                                                              ttGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACTGCAGGGCGAGGAATAGCGTCTGCAGCCAGGGTTTGTGGACCTTGGCGCGAAAAGCCTGATAACCCGCGTCATCGGTGCACAg                                                       >  2:373608/1‑140 (MQ=255)                                                                                      tttGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACTGCAGGGCGAGGAATAGCGTCTGCAGCCAGGGTTTGTGGACCTTGGCGCGAAAAGCCTGATAACCCGCGTCATCGGTGCACAGCGTGTCGc                                               >  1:1293619/1‑140 (MQ=255)                                                                                      tttGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACTGCAGGGCGAGGAATAGCGTCTGCAGCCAGGGTTTGTGGACCTTGGCGCGAAAAGCCTGATAACCCGCGTCATCGGTGCACAGCGTGTCGc                                               <  2:574077/140‑1 (MQ=255)                                                                                      tttGCTGTT‑GCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACTGCAGGGCGAGGAATAGCGTCTGCAGCCAGGGTTTGTGGACCTTGGCGCGAAAAGCCTGATAACCCGCGTCATCGGTGCACAGCGTGTCGcc                                              <  1:73387/140‑1 (MQ=255)                                                                                       ttGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACTGCAGGGCGAGGAATAGCGTCTGCAGCCAGGGTTTGTGGACCTTGGCGCGAAAAGCCTGATAACCCGCGTCATCGGTGCACAGCGTGTCGcc                                              <  1:302679/140‑1 (MQ=255)                                                                                       ttGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACTGCAGGGCGAGGAATAGCGTCTGCAGCCAGGGTTTGTGGACCTTGGCGCGAAAAGCCTGATAACCCGCGTCATCGGTGCACAGCGTGTCGcc                                              <  1:625002/140‑1 (MQ=255)                                                                                       ttGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACTGCAGGGCGAGGAATAGCGTCTGCAGCCAGGGTTTGTGGACCTTGGCGCGAAAAGCCTGATAACCCGCGTCATCGGTGCACAGCGTGTCGcc                                              <  1:820093/140‑1 (MQ=255)                                                                                       ttGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACTGCAGGGCGAGGAATAGCGTCTGCAGCCAGGGTTTGTGGACCTTGGCGCGAAAAGCCTGATAACCCGCGTCATCGGTGCACAGCGTGTCGcc                                              <  1:1613663/140‑1 (MQ=255)                                                                                        tGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACTGCAGGGCGAGGAATAGCGTCTGCAGCCAGGGTTTGTGGACCTTGGCGCGAAAAGCCTGATAACCCGCGTCATCGGTGCACAGCGTGTCGCCa                                             >  1:1432771/1‑140 (MQ=255)                                                                                        tGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACTGCAGGGCGAGGAATAGCGTCTGCAGCCAGGGTTTGTGGACCTTGGCGCGAAAAGCCTGATAACCCGCGTCATCGGTGCACAGCGTGTCGCCa                                             >  2:1473802/1‑140 (MQ=255)                                                                                           tGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACTGCAGGGCGAGGAATAGCGTCTGCAGCCAGGGTTTGTGGACCTTGGCGCGAAAAGCCTGATAACCCGCGTCATCGGTGCACAGCGTGTCGCCATGc                                          <  2:1693465/140‑1 (MQ=255)                                                                                           tGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACTGCAGGGCGAGGAATAGCGTCTGCAGCCAGGGTTTGTGGACCTTGGCGCGAAAAGCCTGATAACCCGCGTCATCGGTGCACAGCGTGTCGCCATGc                                          >  1:1581157/1‑140 (MQ=255)                                                                                            gTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACTGCAGGGCGAGGAATAGCGTCTGCAGCCAGGGTTTGTGGACCTTGGCGCGAAAAGCCTGATAACCCGCGTCATCGGTGCACAGCGTGTCGCCATGCa                                         <  1:1473802/140‑1 (MQ=255)                                                                                               cgcgcgCATTCGCGCGGCAATGCGTTTGCGCACAAACAACTGCAGGGCGAGGAATAGCGTCTGCAGCCAGGGTTTGTGGACCTTGGCGCGAAAAGCCTGATAACCCGCGTCATCGGTGCACAGCGTGTCGCCATGCATaa                                      >  1:501817/1‑140 (MQ=255)                                                                                                       ttCGCGCGGCAATGCGTTTGCGCACAAACAACTGCAGGGCGAGGAATAGCGTCTGCAGCCAGGGTTTGTGGACCTTGGCGCGAAAAGCCTGATAACCCGCGTCATCGGTGCACAGCGTGTCGCCATGCa                                         >  2:1781099/1‑129 (MQ=255)                                                                                                       ttCGCGCGGCAATGCGTTTGCGCACAAACAACTGCAGGGCGAGGAATAGCGTCTGCAGCCAGGGTTTGTGGACCTTGGCGCGAAAAGCCTGATAACCCGCGTCATCGGTGCACAGCGTGTCGCCATGCa                                         <  1:1781099/129‑1 (MQ=255)                                                                                                          gcgcgGCAATGCGTTTGCGCACAAACAACTGCAGGGCGAGGAATAGCGTCTGCAGCCAGGGTTTGTGGACCTTGGCGCGAAAAGCCTGATAACCCGCGTCATCGGTGCACAGCGTGTCGCCATGCATAATCAACACCCgg                           <  2:454901/140‑1 (MQ=255)                                                                                                          gcgcgGCAATGCGTTTGCGCACAAACAACTGCAGGGCGAGGAATAGCGTCTGCAGCCAGGGTTTGTGGACCTTGGCGCGAAAAGCCTGATAACCCGCGTCATCGGTGCACAGCGTGTCGCCATGCATAATCAACACCCgg                           <  2:1233839/140‑1 (MQ=255)                                                                                                                   tGCGTTTGCGCACAAACAACTGCAGGGCGAGGAATAGCGTCTGCAGCCAGGGTTTGTGGACCTTGGCGCGAAAAGCCTGATAACCCGCGTCATCGGTGCACAGCGTGTCGCCATGCATAATCAACACCCGGCGACCATaa                  >  2:622508/1‑140 (MQ=255)                                                                                                                         tGCGCACAAACAACTGCAGGGCGAGGAATAGCGTCTGCAGCCAGGGTTTGTGGACCTTGGCGCGAAAAGCCTGATAACCCGCGTCATCGGTGCACAGCGTGTCGCCATGCATAATCAACACCCGGCGACCATAAAGTTCg            <  1:308549/140‑1 (MQ=255)                                                                                                                          gcgcACAAACAACTGCAGGGCGAGGAATAGCGTCTGCAGCCAGGGTTTGTGGACCTTGGCGCGAAAAGCCTGATAACCCGCGTCATCGGTGCACAGCGTGTCGCCATGCATAATCAACACCCGGCGACCATAAAGTTCGa           >  1:897614/1‑140 (MQ=255)                                                                                                                            gcACAAACAACTGCAGGGCGAGGAATAGCGTCTGCAGCCAGGGTTTGTGGACCTTGGCGCGAAAAGCCTGATAACCCGCGTCATCGGTGCACAGCGTGTCGCCATGCATAATCAACACCCGGCGACCATAAAGTTCGAGc         <  2:1070964/140‑1 (MQ=255)                                                                                                                            gcACAAACAACTGCAGGGCGAGGAATAGCGTCTGCAGCCAGGGTTTGTGGACCTTGGCGCGAAAAGCCTGATAACCCGCGTCATCGGTGCACAGCGTGTCGCCATGCATAATCAACACCCGGCGACCATAAAGTTCGAGc         <  1:971924/140‑1 (MQ=255)                                                                                                                               caaacaaCTGCAGGGCGAGGAATAGCGTCTGCAGCCAGGGTTTGTGGACCTTGGCGCGAAAAGCCTGATAACCCGCGTCATCGGTGCACAGCGTGTCGCCATGCATAATCAACACCCGGCGACCATAAAGTTCGAGCAcc      >  2:107900/1‑140 (MQ=255)                                                                                                                               caaacaaCTGCAGGGCGAGGAATAGCGTCTGCAGCCAGGGTTTGTGGACCTTGGCGCGAAAAGCCTGATAACCCGCGTCATCGGTACACAGCGTGTCGCCATGCATAATCAACACCCGGCGACCATAAAGTTCGAGCAcc      >  2:455407/1‑140 (MQ=255)                                                                                                                                 aacaacTGCAGGGCGAGGAATAGCGTCTGCAGCCAGGGTTTGTGGACCTTGGCGCGAAAAGCCTGATAACCCGCGTCATCGGTGCACAGCGTGTCGCCATGCATAATCAACACCCGGCGACCATAAAGTTCGAGCACCtt    >  2:1555850/1‑140 (MQ=255)                                                                                                                                  acaacTGCAGGGCGAGGAATAGCGTCTGCAGCCAGGGTTTGTGGACCTTGGCGCGAAAAGCCTGATAACCCGCGTCATCGGTGCACAGCGTGTCGCCATGCAT‑ATCAACACCCGGCGACCATAAAGTTCGAGCACCtttt  <  2:1029525/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        ATTGCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACGGCAGGGCGAGGAATAGCGTCTGCAGCCAGGGTTTGTGGACCTTGGCGCGAAAAGCCTGATAACCCGCGTCATCGGTGCACAGCGTGTCGCCATGCATAATCAACACCCGGCGACCATAAAGTTCGAGCACCTTTT  >  NC_000913/553373‑553642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑