Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	728,468	G→T	L89I (CTT→ATT)	kdpA ←	K(+) transporting P‑type ATPase subunit KdpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	728,468	0	G	T	100.0%	318.6 / NA	94	L89I (CTT→ATT)	kdpA	K(+) transporting P‑type ATPase subunit KdpA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (56/38);  total (56/38)

GTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGACGCCAAGTGCGCGAAAAAGTACGC  >  NC_000913/728332‑728602                                                                                                                                         |                                                                                                                                       gTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCgg                                                                                                                                     <  2:561076/140‑1 (MQ=255)  ttAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGc                                                                                                                                    >  2:477534/1‑140 (MQ=255)     aGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGa                                                                                                                                 <  2:1075897/140‑1 (MQ=255)          gCCATCTGGCTGGAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTg                                                                                                                            >  1:211482/1‑140 (MQ=255)          gCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTg                                                                                                                            >  2:969310/1‑140 (MQ=255)            cATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCt                                                                                                                          >  2:797054/1‑140 (MQ=255)            cATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCt                                                                                                                          >  1:1464988/1‑140 (MQ=255)                   cTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAg                                                                                                                   <  1:154089/140‑1 (MQ=255)                   cTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAg                                                                                                                   <  2:625584/140‑1 (MQ=255)                     gAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCa                                                                                                                 <  1:1055202/140‑1 (MQ=255)                       aaTAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAg                                                                                                                   <  1:1591843/136‑1 (MQ=255)                       aaTAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAg                                                                                                                   >  2:1591843/1‑136 (MQ=255)                       aaTAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAAc                                                                                                               <  2:1464988/140‑1 (MQ=255)                        aTAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACa                                                                                                              <  1:797054/140‑1 (MQ=255)                                aCGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAAc                                                                                                      >  1:482417/1‑140 (MQ=255)                                aCGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAAc                                                                                                      >  2:51922/1‑140 (MQ=255)                                 cGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACa                                                                                                     >  1:26795/1‑140 (MQ=255)                                 cGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACa                                                                                                     >  1:1679297/1‑140 (MQ=255)                                    ggTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACGGAGCAACATAAAAAACAGct                                                                                                  <  1:1338193/140‑2 (MQ=255)                                      tttCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATaaaa                                                                                                         >  1:129422/1‑131 (MQ=255)                                      tttCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATaaaa                                                                                                         <  2:129422/131‑1 (MQ=255)                                       ttCACCGCTATAAGATTGCCAGTTGGTATTGGTGAAAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCg                                                                                               <  1:352162/140‑1 (MQ=255)                                        tCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGc                                                                                              <  2:1746966/140‑1 (MQ=255)                                         cACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATaaaa                                                                                                         >  2:1305541/1‑128 (MQ=255)                                         cACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATaaaa                                                                                                         <  1:1305541/128‑1 (MQ=255)                                          aCCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCa                                                                                            >  2:81747/1‑140 (MQ=255)                                            cGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGc                                                                                          <  2:1679297/140‑1 (MQ=255)                                             gCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGcc                                                                                         <  1:1378731/140‑1 (MQ=255)                                               tataAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCACCAGcccc                                                                                       >  2:361528/1‑140 (MQ=255)                                                ataAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCa                                                                                      >  2:927683/1‑140 (MQ=255)                                                 taAGATTGCCATTTGGTATTGGTGACAAAGCTGACGGGGTTATTCGGCGCCAGATCCCACGACAGCCCTGGCAACTGCGGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAg                                                                                     <  2:189614/140‑1 (MQ=255)                                                   aGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCa                                                                                   <  1:1575488/140‑1 (MQ=255)                                                        gCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCg                                                                                               <  2:225852/123‑1 (MQ=255)                                                        gCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCg                                                                                               >  1:225852/1‑123 (MQ=255)                                                        gCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTc                                                                              >  1:729573/1‑140 (MQ=255)                                                        gCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTc                                                                              >  1:108654/1‑140 (MQ=255)                                                         ccAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGcc                                                                                         >  2:96208/1‑128 (MQ=255)                                                         ccAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGcc                                                                                         <  1:96208/128‑1 (MQ=255)                                                         ccAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCa                                                                             >  1:158363/1‑140 (MQ=255)                                                         ccAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCa                                                                             >  1:1490829/1‑140 (MQ=255)                                                         ccAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCa                                                                             >  1:1252883/1‑140 (MQ=255)                                                         ccAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCa                                                                             >  1:1236141/1‑140 (MQ=255)                                                         ccAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCa                                                                             >  2:584955/1‑140 (MQ=255)                                                         ccAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCa                                                                             >  2:574701/1‑140 (MQ=255)                                                           aGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAgg                                                                           >  1:433156/1‑140 (MQ=255)                                                             ttGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGcc                                                                         >  1:1660129/1‑140 (MQ=255)                                                                   ttGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAAt                                                                   <  2:211482/140‑1 (MQ=255)                                                                     ggTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATgg                                                                 <  1:477534/140‑1 (MQ=255)                                                                     ggTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATgg                                                                 <  2:482417/140‑1 (MQ=255)                                                                       tGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGca                                                               <  1:927683/140‑1 (MQ=255)                                                                         aCAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGcaca                                                             >  1:1718718/1‑140 (MQ=255)                                                                          cAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACaa                                                            >  1:1164046/1‑140 (MQ=255)                                                                          cAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACaa                                                            >  2:880175/1‑140 (MQ=255)                                                                           aaaGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCTAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACaaa                                                           >  1:1655818/1‑140 (MQ=255)                                                                           aaaGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCCGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACaaa                                                           >  2:705214/1‑140 (MQ=255)                                                                             aGCTGACGGCGGTATTCTGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGa                                                         >  2:1197062/1‑140 (MQ=255)                                                                             aGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGa                                                         >  1:1647610/1‑140 (MQ=255)                                                                               cTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGata                                                       <  1:957909/140‑1 (MQ=255)                                                                                tGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGatat                                                      <  1:51922/140‑1 (MQ=255)                                                                                 gACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATAtt                                                     <  1:165448/140‑1 (MQ=255)                                                                                 gACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATAtt                                                     >  1:1460522/1‑140 (MQ=255)                                                                                  aCGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTg                                                    >  2:910463/1‑140 (MQ=255)                                                                                      cggTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTc                                                <  1:1247262/140‑1 (MQ=255)                                                                                        gTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCa                                              >  1:119382/1‑140 (MQ=255)                                                                                         tATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAg                                             >  1:90437/1‑140 (MQ=255)                                                                                          tttCGGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGt                                            <  2:52761/139‑1 (MQ=255)                                                                                          tttCAGCGCCAGACCCCACGACAGCCCTGGCAACTGCGGTGGATTAATCGGCAGATATTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGt                                            <  2:1274864/139‑1 (MQ=255)                                                                                            tcggcgcCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTc                                          <  2:814526/137‑1 (MQ=255)                                                                                            tCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTc                                          <  2:26795/140‑1 (MQ=255)                                                                                              aGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCAt                                        >  2:152558/1‑140 (MQ=255)                                                                                                cgcCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCAtct                                      <  1:1197062/140‑1 (MQ=255)                                                                                                  ccAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCa                                    >  1:1274864/1‑140 (MQ=255)                                                                                                   cAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCAc                                   <  2:729573/140‑1 (MQ=255)                                                                                                   cAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCAc                                   >  1:77982/1‑140 (MQ=255)                                                                                                    aGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGAAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACg                                  <  2:1236141/140‑1 (MQ=255)                                                                                                      aTCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGt                                <  2:1718718/140‑1 (MQ=255)                                                                                                      aTCCCACGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGt                                <  1:584955/140‑1 (MQ=255)                                                                                                            cGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCAtct                                      >  1:299556/1‑128 (MQ=255)                                                                                                            cGACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCAtct                                      <  2:299556/128‑1 (MQ=255)                                                                                                             gACAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGAc                         >  1:1245616/1‑140 (MQ=255)                                                                                                               cAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAATACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGACGc                       >  1:589363/1‑140 (MQ=255)                                                                                                               cAGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGACGc                       >  1:703958/1‑140 (MQ=255)                                                                                                                aGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCaga                            >  1:1463555/1‑134 (MQ=255)                                                                                                                aGCCCTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCaga                            <  2:1463555/134‑1 (MQ=255)                                                                                                                  cccTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGACGCCaa                    >  2:104933/1‑140 (MQ=255)                                                                                                                  cccTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGACGCCaa                    >  1:999730/1‑140 (MQ=255)                                                                                                                  cccTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGACGCCaa                    >  1:888687/1‑140 (MQ=255)                                                                                                                  cccTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGACGCCaa                    >  1:205784/1‑140 (MQ=255)                                                                                                                  cccTGGCAACTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGACGCCaa                    >  1:1226169/1‑140 (MQ=255)                                                                                                                       gCAACTGCTGGGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGACGCCAAGTgcg               <  2:565330/140‑1 (MQ=255)                                                                                                                         aaCTGCTGTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGACGCCAAGTGcgcg             <  1:574701/140‑1 (MQ=255)                                                                                                                           ctgctgTGGATTAATCGCCAGATAGTGCTGCCCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGACGCCAAGTGCGCGaa           <  1:361528/140‑1 (MQ=255)                                                                                                                            tgctgTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGACGCCAAGTGCGCGaaa          >  1:1307302/1‑140 (MQ=255)                                                                                                                            tgctgTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGACGCCAAGTGCGCGaaa          >  1:945915/1‑140 (MQ=255)                                                                                                                            tgctgTGGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGACGCCAAGTGCGCGaaa          >  2:120038/1‑140 (MQ=255)                                                                                                                                 tgGATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGACGCCAAGTGCGCGAAAAAGTa     >  2:23931/1‑140 (MQ=255)                                                                                                                                   gATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGACGCCAAGTGCGCGAAAAAGTAcg   <  2:1164046/140‑1 (MQ=255)                                                                                                                                   gATTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGACGCCAAGTGCGCGAAAAAGTAcg   >  1:279832/1‑140 (MQ=255)                                                                                                                                   gATTAACCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGACGCCAAGTGCGCGAAAAAGTAcg   <  2:1245616/140‑1 (MQ=255)                                                                                                                                    aTTAATCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGACGCCAAGTGCGCGAAAAAGTAcgc  <  2:1660129/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       GTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGACGCCAAGTGCGCGAAAAAGTACGC  >  NC_000913/728332‑728602

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑