Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,360,746	G→T	R55S (CGT→AGT)	puuA ←	glutamate‑putrescine ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,360,746	0	G	T	100.0%	214.1 / NA	61	R55S (CGT→AGT)	puuA	glutamate‑putrescine ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (39/22);  total (39/22)

CAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTT  >  NC_000913/1360610‑1360881                                                                                                                                         |                                                                                                                                        caGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACTCtt                                                                                                                                      >  2:1552830/1‑140 (MQ=255)      aCGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATActct                                                                                                                                       >  2:279125/1‑134 (MQ=255)      aCGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACTCTTTCCAc                                                                                                                                 >  1:569357/1‑140 (MQ=255)          tCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACTCTTTCCACGGaa                                                                                                                             >  1:1039381/1‑140 (MQ=255)          tCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACTCTTTCCACGGaa                                                                                                                             <  2:1217487/140‑1 (MQ=255)            cGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACTCTTTCCACGGAAGc                                                                                                                           <  2:1081209/140‑1 (MQ=255)            cGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACTCTTTCCACGGAAGc                                                                                                                           >  1:1518916/1‑140 (MQ=255)              gCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACTCTTTCCACGGAAGCaa                                                                                                                         >  1:470303/1‑140 (MQ=255)               cTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACTCTTTCCACGGAAGCAAc                                                                                                                        >  1:1495117/1‑140 (MQ=255)               cTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACTCTTTCCACGGAAGCAAc                                                                                                                        >  2:1009784/1‑140 (MQ=255)                  gCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACTCTTTCCACGGAAGCAACCAt                                                                                                                     <  1:1477455/140‑1 (MQ=255)                        ttCCTGACCCAGACCTGCTTCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACTCTTTCCACGGAAGCAACCATTTAAAt                                                                                                               <  2:705074/140‑1 (MQ=255)                          ccTGACCCAGACCTGCTTCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACTCTTTCCACGGAAGCAACCATTTAAATCg                                                                                                             <  2:5649/140‑1 (MQ=255)                           cTGACCCAGACCTGCTTCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACTCTTTCCACGGAAGCAACCATTTAAATCgg                                                                                                            <  1:1552830/140‑1 (MQ=255)                             gACCCAGACCTGCTTCTTCTACTACGCTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACTCTTTCCACGGAAGCAACCATTTACATCGgta                                                                                                          >  1:1769549/1‑140 (MQ=255)                              aCCCAGACCTGCTTCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACTCTTTCCACGGAAGCAACCATTTAAATCGgtaa                                                                                                         >  1:1792937/1‑140 (MQ=255)                              aCCCAGACCTGCTTCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACTCTTTCCACGGAAGCAACCATTTAAATCGgtaa                                                                                                         >  1:1567995/1‑140 (MQ=255)                              aCCCAGACCTGCTTCTTCTACTACGTTGCCCAGAATATCCATTACAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACTCTTTCCACGGAAGCAACCATTTAAATCGgtaa                                                                                                         >  2:490993/1‑140 (MQ=255)                                  aGACCTGCTTCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACTCTTTCCACGGAAGCAACCATTTAAATCGgtaagtaa                                                                                                     <  1:464968/140‑1 (MQ=255)                                         cttcttCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACTCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCa                                                                                              >  1:1274242/1‑140 (MQ=255)                                                      gTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACTCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTAt                                                                                 <  2:1110744/140‑1 (MQ=255)                                                       ttGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACTCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTAtt                                                                                >  2:151194/1‑140 (MQ=255)                                                        tGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACTCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTc                                                                               >  2:200980/1‑140 (MQ=255)                                                        tGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACTCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTc                                                                               >  1:642306/1‑140 (MQ=255)                                                        tGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACTCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTc                                                                               >  1:524849/1‑140 (MQ=255)                                                          cccAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACTCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCgg                                                                             >  1:777541/1‑140 (MQ=255)                                                          cccAGAATATCCATTGCAAATAACGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGGATACTCTTTCCACGGAAGCAACCATTTAACTCGGTAAGTAAAACATCAACATATTGCGTATTCgg                                                                             >  2:439358/1‑140 (MQ=255)                                                            cAGAATATCCATTGCAAATACCGAGGCCGTGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACTCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGt                                                                           >  1:693242/1‑140 (MQ=255)                                                             aGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACTCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTa                                                                          <  1:280181/140‑1 (MQ=255)                                                             aGAATATCCATTACAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACTCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTa                                                                          <  1:490993/140‑1 (MQ=255)                                                                    ccATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACTCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTcc                                                                   >  1:68838/1‑140 (MQ=255)                                                                    ccATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACTCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTcc                                                                   >  2:29487/1‑140 (MQ=255)                                                                        tGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACTCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTa                                                                          <  2:852195/129‑1 (MQ=255)                                                                        tGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACTCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTa                                                                          >  1:852195/1‑129 (MQ=255)                                                                         gCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACTCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTa                                                                          >  2:1171987/1‑128 (MQ=255)                                                                         gCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACTCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTa                                                                          <  1:1171987/128‑1 (MQ=255)                                                                         gCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACTCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAgcgc                                                                      >  1:1431723/1‑132 (MQ=255)                                                                         gCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACTCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAgcgc                                                                      <  2:1431723/132‑1 (MQ=255)                                                                         gCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACTCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTa                                                              >  1:1052363/1‑140 (MQ=255)                                                                         gCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACTCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTa                                                              >  2:1158644/1‑140 (MQ=255)                                                                         gCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACTCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTa                                                              >  2:116437/1‑140 (MQ=255)                                                                           aaaTACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACTCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGc                                                            <  2:1098934/140‑1 (MQ=255)                                                                           aaaTACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACTCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGc                                                            <  2:1274242/140‑1 (MQ=255)                                                                           aaaTACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACTCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGc                                                            <  1:200980/140‑1 (MQ=255)                                                                             aTACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACTCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGt                                                          <  1:439358/140‑1 (MQ=255)                                                                               aCCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACTCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGttt                                                        >  1:277840/1‑140 (MQ=255)                                                                                   aGGCCGGGCAATAACACCCTTTCTCGGGCTTCTTCAGGCGTGAAACAGGAATACTCTTTCCACGGAATCAACCATTTAAACCGGCAAGCAAAACATCAACATATTGCGTATTCGGGTACCGCTCCAGCTAGCGATTCACt                                                    >  2:1086569/1‑140 (MQ=255)                                                                                   aGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACTCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACt                                                    >  1:51732/1‑140 (MQ=255)                                                                                   aGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACTCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACt                                                    <  2:1495117/140‑1 (MQ=255)                                                                                    ggCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACTCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACtt                                                   >  1:75332/1‑140 (MQ=255)                                                                                    ggCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACTCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACtt                                                   >  1:47949/1‑140 (MQ=255)                                                                                     gCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACTCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTc                                                  <  2:75332/140‑1 (MQ=255)                                                                                     gCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACTCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTc                                                  <  2:41262/140‑1 (MQ=255)                                                                                     gCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACTCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTc                                                  >  1:1544466/1‑140 (MQ=255)                                                                                      ccGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACTCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTcc                                                 <  1:1009784/140‑1 (MQ=255)                                                                                           aaaTAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACTCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGt                                            <  2:904103/140‑1 (MQ=255)                                                                                                      tttCTCGAGCTTCTTCAGGCTTGAAACAGGAATACTCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTAc                                 >  2:224450/1‑140 (MQ=255)                                                                                                       ttCTCGAGCTTCTTCAGGCTTGAAACAGGAATACTCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTAcc                                >  1:1357408/1‑140 (MQ=255)                                                                                                        tctcGAGCTTCTTCAGGCTTGAAACAGGAATACTCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTAccc                               >  1:669960/1‑140 (MQ=255)                                                                                                        tctcGAGCTTCTTCAGGCTTGAAACAGGAATACTCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCG‑TTCACTTCCTGCGTAAAGGCGCTAcccc                              >  1:896269/1‑140 (MQ=255)                                                                                                                            gAAACAGGAATACTCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCTCTTCTGActgctg           >  2:1428211/1‑140 (MQ=255)                                                                                                                            gAAACAGGAATACTCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCTCTTCTGActgctg           >  1:829259/1‑140 (MQ=255)                                                                                                                            gAAACAGGAATACTCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCTCTTCTGActgctg           >  1:763149/1‑140 (MQ=255)                                                                                                                                 agaaaTACTCTTCCCACGAAAGCAACCATTAAAATGGGAAAGAAAAACATAAACATATTGCGTTTTGGGGTGGCGCTCCGGGTAGCTTTTCCCTCCCTGCGTAAAGGCGCTCCCCCGCCTCTCTTCTGACTGCTGAACaa      <  2:68838/137‑1 (MQ=255)                                                                                                                                    aaTACTCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGt   >  2:1451177/1‑140 (MQ=255)                                                                                                                                     aTACTCTTCCCAGGGAAGCAACCATTTAAATCGGTAAGAAAAACATCAACATATTGCGTATTGGGGTAGCGCTCCAGGTAGCTTTTCACTTCCTGGGAAAGGGCCCTCCCCCGCCTCTCTTCTGACTGCTGAACAAAGtt  <  2:1544466/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        CAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTT  >  NC_000913/1360610‑1360881

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑