Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,155,372	G→T	L6L (CTG→CTT)	yqhD →	NADPH‑dependent aldehyde reductase YqhD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,155,372	0	G	T	100.0%	327.6 / NA	91	L6L (CTG→CTT)	yqhD	NADPH‑dependent aldehyde reductase YqhD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (52/39);  total (52/39)

ATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTGCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGTTTACGCGAACAAATTCCTCACGATGCTCGCGTATTGATTACCTACGGCGGCGGCAGCGTGAAAAAAACCGGCGTTCTCGATCAAGTTCTGG  >  NC_000913/3155239‑3155508                                                                                                                                      |                                                                                                                                         aTAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACAcc                                                                                                                                    >  1:1744481/1‑140 (MQ=255) aTAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACAcc                                                                                                                                    >  1:1696791/1‑140 (MQ=255)  tAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACAccc                                                                                                                                   >  1:474262/1‑140 (MQ=255)   aGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACAcccc                                                                                                                                  <  1:1507714/140‑1 (MQ=255)    ggCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCa                                                                                                                                 >  2:275465/1‑140 (MQ=255)    ggCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCa                                                                                                                                 >  1:1862976/1‑140 (MQ=255)    ggCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCa                                                                                                                                 >  1:1693021/1‑140 (MQ=255)    ggCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCa                                                                                                                                 >  2:807930/1‑140 (MQ=255)     gCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCaa                                                                                                                                <  2:1218420/140‑1 (MQ=255)      cAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCAAc                                                                                                                               >  1:2118136/1‑140 (MQ=255)         gACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCg                                                                                                                            <  2:1721836/140‑1 (MQ=255)           cATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCa                                                                                                                          <  1:518235/140‑1 (MQ=255)           cATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCa                                                                                                                          <  1:1035183/140‑1 (MQ=255)             ttGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCAtt                                                                                                                        <  2:847686/140‑1 (MQ=255)             ttGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCAtt                                                                                                                        <  2:543602/140‑1 (MQ=255)             ttGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCAtt                                                                                                                        <  2:340538/140‑1 (MQ=255)             ttGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCAtt                                                                                                                        <  2:61794/140‑1 (MQ=255)                   gAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCATTCTGttt                                                                                                                  <  1:1555999/140‑1 (MQ=255)                      aTGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGt                                                                                                               >  1:1971202/1‑140 (MQ=255)                      aTGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGt                                                                                                               <  2:1360861/140‑1 (MQ=255)                         aGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGTaaa                                                                                                            >  2:1734579/1‑140 (MQ=255)                           cATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAgg                                                                                                          >  2:336628/1‑140 (MQ=255)                                  agCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATc                                                                                                   >  1:751882/1‑140 (MQ=255)                                   gCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCg                                                                                                  >  1:2035716/1‑140 (MQ=255)                                    ccAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCATACTGTTTGGTAAAGGCGCAATCGc                                                                                                 <  2:541521/140‑1 (MQ=255)                                        ggCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGt                                                                                             >  1:1314021/1‑140 (MQ=255)                                        ggCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGt                                                                                             >  1:531976/1‑140 (MQ=255)                                        ggCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGt                                                                                             >  1:306537/1‑140 (MQ=255)                                        ggCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGt                                                                                             >  1:2168824/1‑140 (MQ=255)                                            cTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGTTTAc                                                                                         >  2:1471661/1‑140 (MQ=255)                                             tGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGcaca                                                                                                      >  2:1074171/1‑126 (MQ=255)                                             tGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGcaca                                                                                                      <  1:1074171/126‑1 (MQ=255)                                              ggCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGTTTAcgc                                                                                       <  2:1693021/140‑1 (MQ=255)                                              ggCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGTTTAcgc                                                                                       >  1:289958/1‑140 (MQ=255)                                              ggCGATCACAATGAAAAACATCACGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGTTTAcgc                                                                                       <  2:2083853/140‑1 (MQ=255)                                                  aTCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGTTTACGCGAAc                                                                                   <  1:1158051/140‑1 (MQ=255)                                                       aaTGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGTTTACGCGAACAAAtt                                                                              <  2:1506962/140‑1 (MQ=255)                                                         tGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGTTTACGCGAACAAATTcc                                                                            >  1:2075421/1‑140 (MQ=255)                                                         tGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGTTTACGCGAACAAATTcc                                                                            >  1:740263/1‑140 (MQ=255)                                                          gAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGTTTACGCGAACAAATTCCt                                                                           <  2:1490917/140‑1 (MQ=255)                                                              aaCATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGTTTAcgc                                                                                       <  2:2148506/124‑1 (MQ=255)                                                              aaCATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGTTTAcgc                                                                                       >  1:2148506/1‑124 (MQ=255)                                                                 aTCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGTTTACGCGAACAAATTCCTCACGATg                                                                    <  2:2168824/140‑1 (MQ=255)                                                                 aTCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGTTTACGCGAACAAATTCCTCACGATg                                                                    <  2:268857/140‑1 (MQ=255)                                                                 aTCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGTTTACGCGAACAAATTCCTCACGATg                                                                    >  1:2079385/1‑140 (MQ=255)                                                                   cAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGTTTACGCGAACAAATTCCTCACGATGCt                                                                  <  2:474262/140‑1 (MQ=255)                                                                       cAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGTTTACGCGAACAAATTCCTCACGATGCTcgcg                                                              <  2:561423/140‑1 (MQ=255)                                                                       cAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGTTTACGCGAACAAATTCCTCACGATGCTcgcg                                                              <  2:1744481/140‑1 (MQ=255)                                                                       cAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGTTTACGCGAACAAATTCCTCACGATGCTcgcg                                                              <  1:1734579/140‑1 (MQ=255)                                                                       cAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGTTTACGCGAACAAATTCCTCACGATGCTcgcg                                                              <  1:807930/140‑1 (MQ=255)                                                                        aGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGTTTACGCGAACAAATTCCTCACGATGCTCGCGt                                                             <  2:1339334/140‑1 (MQ=255)                                                                        aGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGTTTACGCGAACAAATTCCTCACGATGCTCGCGt                                                             <  2:603657/140‑1 (MQ=255)                                                                          aTCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGTTTACGCGAACAAATTCCTCACGATGCTCGCGTAt                                                           >  1:1114138/1‑140 (MQ=255)                                                                          aTCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGTTTACGCGAACAAATTCCTCACGATGCTCGCGTAt                                                           >  1:1820414/1‑140 (MQ=255)                                                                           tCGTTCTCTGCCCTCATATTGGTCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGTTTACGCGAACAAATTCCTCACGATGCt                                                                  >  2:1160782/1‑132 (MQ=255)                                                                           tCGTTCTCTGCCCTCATATTGGTCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGTTTACGCGAACAAATTCCTCACGATGCt                                                                  <  1:1160782/132‑1 (MQ=255)                                                                             gTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGTTTACGCGAACAAATTCCTCACGATGCTCGCGTATTGa                                                        <  2:1114138/140‑1 (MQ=255)                                                                             gTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGTTTACGCGAACAAATTCCTCACGATGCTCGCGTATTGa                                                        <  1:1471661/140‑1 (MQ=255)                                                                                ctctGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGTTTACGCGAACAAATTCCTCACGATGCTCGCGTATTGATTa                                                     >  2:359656/1‑140 (MQ=255)                                                                                  ctGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGTTTACGCGAACAAATTCCTCACGATGCTCGCGTATTGATTAcc                                                   >  1:1597716/1‑140 (MQ=255)                                                                                    gCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGTTTACGCGAACAAATTCCTCACGATGCTCGCGTATTGATTACCTa                                                 >  1:1512527/1‑140 (MQ=255)                                                                                    gCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGTTTACGCGAACAAATTCCTCACGATGCTCGCGTATTGATTACCTa                                                 >  1:225584/1‑140 (MQ=255)                                                                                     cccTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGTTTACGCGAACAAATTCCTCACGATGCTCGCGTATTGATTACCTAc                                                >  1:281153/1‑140 (MQ=255)                                                                                     cccTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGTTTACGCGAACAAATTCCTCACGATGCTCGCGTATTGATTACCTAc                                                >  1:460447/1‑140 (MQ=255)                                                                                        tCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGTTTACGCGAACAAATTCCTCACGATGCTCGCGTATTGATTACCTAcggc                                             >  1:833021/1‑140 (MQ=255)                                                                                                gCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGTTTACGCGAACAAATTCCTCACGATGCTCGCGTATTGATTACCTACGGCGGCGGCAg                                     >  1:1590944/1‑140 (MQ=255)                                                                                                gCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGTTTACGCGAACAAATTCCTCACGATGCTCGCGTATTGATTACCTACGGCGGCGGCAg                                     >  1:378194/1‑140 (MQ=255)                                                                                                gCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGTTTACGCGAACAAATTCCTCACGATGCTCGCGTATTGATTACCTACGGCGGCGGCAg                                     >  1:383408/1‑140 (MQ=255)                                                                                                    aGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACGCCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGTTTACGCGAACAAATTCCTCACGATGCTCGCGTATTGATTACCTACGGCGGCGGCAGCGTg                                 >  1:246364/1‑140 (MQ=255)                                                                                                    aGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGTTTACGCGAACAAATTCCTCACGATGCTCGCGTATTGATTACCTACGGCGGCGGCAGCGTg                                 >  2:834712/1‑140 (MQ=255)                                                                                                    aGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGTTTACGCGAACAAATTCCTCACGATGCTCGCGTATTGATTACCTACGGCGGCGGCAGCGTg                                 >  1:617613/1‑140 (MQ=255)                                                                                                    aGCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGTTTACGCGAACAAATTCCTCACGATGCTCGCGTATTGATTACCTACGGCGGCGGCAGCGTg                                 >  1:1691059/1‑140 (MQ=255)                                                                                                     gCAAAGGGAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGTTTACGCGAACAAATTCCTCACGATGCTCGCGTATTGATTACCTACGGCGGCGGCAGCGTGa                                >  1:2051079/1‑140 (MQ=255)                                                                                                          gggAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGTTTACGCGAACAAATTCCTCACGATGCTCGCGTATTGATTACCTACGGCGGCGGCAGCGTGaaaaaa                           >  1:167449/1‑140 (MQ=255)                                                                                                          gggAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGTTTACGCGAACAAATTCCTCACGATGCTCGCGTATTGATTACCTACGGCGGCGGCAGCGTGaaaaaa                           >  1:999121/1‑140 (MQ=255)                                                                                                           ggAGCAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGTTTACGCGAACAAATTCCTCACGATGCTCGCGTATTGATTACCTACGGCGGCGGCAGCGTGaaaaaaa                          >  1:2096025/1‑140 (MQ=255)                                                                                                               cAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGTTTACGCGAACAAATTCCTCACGATGCTCGCGTATTGATTACCTACGGCGGCGGCAGCGTGAAAAAAACCgg                      >  1:2149049/1‑140 (MQ=255)                                                                                                               cAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGTTTACGCGAACAAATTCCTCACGATGCTCGCGTATTGATTACCTACGGCGGCGGCAGCGTGAAAAAAACCgg                      <  2:1512527/140‑1 (MQ=255)                                                                                                               cAAGTAATGAACAACTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGTTTACGCGAACAAATTCCTCACGATGCTCGCGTATTGATTACCTACGGCGGCGGCAGCGTGAAAAAAACCgg                      >  1:392209/1‑140 (MQ=255)                                                                                                                aaGTAATGAACAACTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGTTTACGCGAACAAATTCCTCACGATGCTCGCGTATTGATTACCTACGGCGGCGGCAGCGTGAAAAAAACCGGc                     >  2:718912/1‑140 (MQ=255)                                                                                                                  gTAATGAACAACTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGTTTACGCGAACAAATTCCTCACGATGCTCGCGTATTGATTACCTACGGCGGCGGCAGCGTGAAAAAAACCGGCGt                   >  1:1038527/1‑140 (MQ=255)                                                                                                                            acTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGTTTACGCGAACAAATTCCTCACGATGCTCGCGTATTGATTACCTACGGCGGCGGCAGCGTGAAAAAAACCGGCGTTCTCGATCaa         >  1:1545307/1‑140 (MQ=255)                                                                                                                             cTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGTTTACGCGAACAAATTCCTCACGATGCTCGCGTATTGATTACCTACGGCGGCGGCAGCGTGAAAAAAACCGGCGTTCTCGATCAAg        <  2:306537/140‑1 (MQ=255)                                                                                                                             cTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGTTTACGCGAACAAATTCCTCACGATGCTCGCGTATTGATTACCTACGGCGGCGGCAGCGTGAAAAAAACCGGCGTTCTCGATCAAg        >  1:1925181/1‑140 (MQ=255)                                                                                                                             cTTTAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGTTTACGCGAACAAATTCCTCACGATGCTCGCGTATTGATTACCTACGGCGGCGGCAGCGTGAAAAAAACCGGCGTTCTCGATCAAg        >  2:744271/1‑140 (MQ=255)                                                                                                                              tttAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGTTTACGCGAACAAATTCCTCACGATGCTCGCGTATTGATTACCTACGGCGGCGGCAGCGTGAAAAAAACCGGCGTTCTCGATCAAGt       <  2:2096025/140‑1 (MQ=255)                                                                                                                              tttAATCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGTTTACGCGAACAAATTCCTCACGATGCTCGCGTATTGATTACCTACGGCGGCGGCAGCGTGAAAAAAACCGGCGTTCTCGATCAAGt       <  2:2118136/140‑1 (MQ=255)                                                                                                                                   tCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGTTTACGCGAACAAATTCCTCACGATGCTCGCGTATTGATTACCTACGGCGGCGGCAGCGTGAAAAAAACCGGCGTTCTCGATCAAGTTCTgg  <  2:1545307/140‑1 (MQ=255)                                                                                                                                   tCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGTTTACGCGAACAAATTCCTCACGATGCTCGCGTATTGATTACCTACGGCGGCGGCAGCGTGAAAAAAACCGGCGTTCTCGATCAAGTTCTgg  <  2:289958/140‑1 (MQ=255)                                                                                                                                   tCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGTTTACGCGAACAAATTCCTCACGATGCTCGCGTATTGATTACCTACGGCGGCGGCAGCGTGAAAAAAACCGGCGTTCTCGATCAAGTTCTgg  <  2:1862976/140‑1 (MQ=255)                                                                                                                                   tCTTCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGTTTACGCGAACAAATTCCTCACGATGCTCGCGTATTGATTACCTACGGCGGCGGCAGCGTGAAAAAAACCGGCGTTCTCGATCAAGTTCTgg  <  2:2075421/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                         ATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTGCACACCCCAACCCGCATTCTGTTTGGTAAAGGCGCAATCGCTGGTTTACGCGAACAAATTCCTCACGATGCTCGCGTATTGATTACCTACGGCGGCGGCAGCGTGAAAAAAACCGGCGTTCTCGATCAAGTTCTGG  >  NC_000913/3155239‑3155508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑