| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 646,747 | 0 | T | G | 29.0% | 52.0 / 15.4 | 31 | T255P (ACC→CCC) | citG | triphosphoribosyl‑dephospho‑CoA synthase |
| Reads supporting (aligned to +/- strand): ref base T (2/20); new base G (9/0); total (11/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.73e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCA > NC_000913/646614‑646886 | aTCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAAt > 1:334299/1‑140 (MQ=255)aTCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAAt > 2:163519/1‑140 (MQ=255) gCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCt < 2:36965/140‑1 (MQ=255) tgCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGGCGGATGGCCCCCTTTTTCAATAATAttttgg > 2:877849/1‑137 (MQ=255) aaaaaCCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTc < 1:411263/140‑1 (MQ=255) aCCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCgcgc < 1:200662/140‑1 (MQ=255) aCCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCgcgc < 1:323730/140‑1 (MQ=255) ccAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCt < 1:877849/140‑1 (MQ=255) ggTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGGATTCCCCCCTTTTTTAAAAATTTTTTTGGCTCCCGCtttg > 2:200662/1‑137 (MQ=255) gTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATGCCCCCCTTTTTCAATAATGTTTTCGCCCCGCGCTGTAg > 2:323730/1‑140 (MQ=255) aGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGTGGCTTAATGTCCCCCCTTTGTAATTATCTATTCGCCTCGCGCTGTaagac > 2:410360/1‑136 (MQ=255) gTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTTGGATGCCCCCCTTTTTTAATAAATTTTTCGCCCCGCCCCGTTGCCCGCGCCgg > 2:841793/1‑140 (MQ=255) gTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATGCCCCCCTTTTTCAATAATGTTTTCGCCCCGCGCTGTAGCCCGCGCAgg > 1:619143/1‑140 (MQ=255) aGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACATTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGcc < 1:769605/140‑1 (MQ=255) gTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATGGCCCCCTTTTTCAATAATTTTTTCGCCTCCCGCTGTTGCCAGCGCAGGcccc > 1:392693/1‑140 (MQ=255) gTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATGCCCCCCTTTTTCAATAATATTTTTGCCCCCCCCTGTTGCCAGCGCCGGgccc > 2:472478/1‑140 (MQ=255) cAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATGCCCCCCTTTTTCAATAATTTTTTCGCCCCCCCCCGTAGCCAGCGCAGGcccccc > 2:84359/1‑140 (MQ=255) tGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCa < 2:268546/140‑1 (MQ=255) ccTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACcgcg < 2:419691/140‑1 (MQ=255) gACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGAt < 2:334299/140‑1 (MQ=255) aCTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTACAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATg < 2:659860/140‑1 (MQ=255) cTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGc < 2:725882/140‑1 (MQ=255) aTTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGt < 2:683447/140‑1 (MQ=255) gacagATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTa < 1:497762/136‑1 (MQ=255) tCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGGATGCCCCCCTTTTTTAATAATATTTTCGCCTCCCCCTGTAGCCAGCGCCGGCCCCCCCCCCCCCCCCCCCGTGCCACGGTGGTATCGcccctggt > 2:843486/1‑134 (MQ=255) ccTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTTGGATGCCCCCCTTTTTTCATAATGTTTTTGCCCCCCCCCGTAGGCCGCGCCGGCCCCCCCCCCCCCCCCCCCCTGCAACGGTGGTATTGCCCCTGATcc > 2:303681/1‑139 (MQ=255) tGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGcc < 1:843486/140‑1 (MQ=255) gTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTTGGAGGTCCCCCTTTTTTAATAATGTTTTCGCCCCCCGCTGTAGCCAGCGCAGGCCCCCCCCCCCCCCCCGCGATGCAACGTTGGTATCGCCCCTGAACGccc > 2:251724/1‑139 (MQ=255) gTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTGGAAAGCCCCCCTTTTGCAATAATGTTTGTGCCCCCCCCCGTAGGCAGCCCAGGCCCCCCCCCCCCCCCCGCCGTGCAACGGTGGGATCGCCGGTGATCGccc > 2:273139/1‑139 (MQ=255) gTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGGATGCCCCCCTTTTTCCATAAATTTTTCGCCCCCCGCCGGAGGCCGCGCCGGCCCCCCCCCCCCCCCCCCGCTGCCACGTTGGGATTGCCGTTGATCGccc > 2:375598/1‑139 (MQ=255) aCTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGt < 2:161919/140‑1 (MQ=255) aCTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGt < 1:858989/140‑1 (MQ=255) tGCCGGAGATAATCGAGATCGGCGGGGGGTCGGATGCCCCCCTTTTTCAATAATATTTTCGCCCCCCGCTGGAGCCAGCGCCGGCCCCCCCCCCCCCCCCGCGATGCAACCTTGGTATCGCCGTTGTTCGCCCTCAGTAg > 2:126322/1‑140 (MQ=255) ccGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGa < 1:163519/140‑1 (MQ=255) aaTCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTa < 1:126322/140‑1 (MQ=255) aaTCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTa < 1:273139/140‑1 (MQ=255) ggTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGtt < 2:767600/140‑1 (MQ=255) ttCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCa < 1:503010/140‑1 (MQ=255) | ATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCA > NC_000913/646614‑646886 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |