Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913646,7600TC26.7% 48.1 / 10.7 30K250K (AAA→AAG) citGtriphosphoribosyl‑dephospho‑CoA synthase
Reads supporting (aligned to +/- strand):  ref base T (1/21);  new base C (8/0);  total (9/21)
Fisher's exact test for biased strand distribution p-value = 1.54e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAA  >  NC_000913/646621‑646894
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gCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCt                                                                                                                                        <  2:36965/140‑1 (MQ=255)
                   tgCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGGCGGATGGCCCCCTTTTTCAATAATAttttgg                                                                                                                     >  2:877849/1‑137 (MQ=255)
                       aaaaaCCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTc                                                                                                                 <  1:411263/140‑1 (MQ=255)
                           aCCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCgcgc                                                                                                             <  1:200662/140‑1 (MQ=255)
                           aCCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCgcgc                                                                                                             <  1:323730/140‑1 (MQ=255)
                            ccAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCt                                                                                                            <  1:877849/140‑1 (MQ=255)
                               ggTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGGATTCCCCCCTTTTTTAAAAATTTTTTTGGCTCCCGCtttg                                                                                                         >  2:200662/1‑137 (MQ=255)
                                gTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATGCCCCCCTTTTTCAATAATGTTTTCGCCCCGCGCTGTAg                                                                                                        >  2:323730/1‑140 (MQ=255)
                                   aGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGTGGCTTAATGTCCCCCCTTTGTAATTATCTATTCGCCTCGCGCTGTaagac                                                                                                     >  2:410360/1‑136 (MQ=255)
                                          gTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTTGGATGCCCCCCTTTTTTAATAAATTTTTCGCCCCGCCCCGTTGCCCGCGCCgg                                                                                              >  2:841793/1‑140 (MQ=255)
                                          gTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATGCCCCCCTTTTTCAATAATGTTTTCGCCCCGCGCTGTAGCCCGCGCAgg                                                                                              >  1:619143/1‑140 (MQ=255)
                                            aGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACATTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGcc                                                                                            <  1:769605/140‑1 (MQ=255)
                                              gTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATGGCCCCCTTTTTCAATAATTTTTTCGCCTCCCGCTGTTGCCAGCGCAGGcccc                                                                                          >  1:392693/1‑140 (MQ=255)
                                              gTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATGCCCCCCTTTTTCAATAATATTTTTGCCCCCCCCTGTTGCCAGCGCCGGgccc                                                                                          >  2:472478/1‑140 (MQ=255)
                                                cAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATGCCCCCCTTTTTCAATAATTTTTTCGCCCCCCCCCGTAGCCAGCGCAGGcccccc                                                                                        >  2:84359/1‑140 (MQ=255)
                                                      tGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCa                                                                                  <  2:268546/140‑1 (MQ=255)
                                                           ccTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACcgcg                                                                             <  2:419691/140‑1 (MQ=255)
                                                               gACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGAt                                                                         <  2:334299/140‑1 (MQ=255)
                                                                aCTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTACAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATg                                                                        <  2:659860/140‑1 (MQ=255)
                                                                 cTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGc                                                                       <  2:725882/140‑1 (MQ=255)
                                                                      aTTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGt                                                                  <  2:683447/140‑1 (MQ=255)
                                                                           gacagATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTa                                                             <  1:497762/136‑1 (MQ=255)
                                                                                      tCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGGATGCCCCCCTTTTTTAATAATATTTTCGCCTCCCCCTGTAGCCAGCGCCGGCCCCCCCCCCCCCCCCCCCGTGCCACGGTGGTATCGcccctggt                                                  >  2:843486/1‑134 (MQ=255)
                                                                                        ccTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTTGGATGCCCCCCTTTTTTCATAATGTTTTTGCCCCCCCCCGTAGGCCGCGCCGGCCCCCCCCCCCCCCCCCCCCTGCAACGGTGGTATTGCCCCTGATcc                                                >  2:303681/1‑139 (MQ=255)
                                                                                          tGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGcc                                              <  1:843486/140‑1 (MQ=255)
                                                                                           gTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTTGGAGGTCCCCCTTTTTTAATAATGTTTTCGCCCCCCGCTGTAGCCAGCGCAGGCCCCCCCCCCCCCCCCGCGATGCAACGTTGGTATCGCCCCTGAACGccc                                             >  2:251724/1‑139 (MQ=255)
                                                                                           gTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTGGAAAGCCCCCCTTTTGCAATAATGTTTGTGCCCCCCCCCGTAGGCAGCCCAGGCCCCCCCCCCCCCCCCGCCGTGCAACGGTGGGATCGCCGGTGATCGccc                                             >  2:273139/1‑139 (MQ=255)
                                                                                           gTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGGATGCCCCCCTTTTTCCATAAATTTTTCGCCCCCCGCCGGAGGCCGCGCCGGCCCCCCCCCCCCCCCCCCGCTGCCACGTTGGGATTGCCGTTGATCGccc                                             >  2:375598/1‑139 (MQ=255)
                                                                                                aCTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGt                                        <  2:161919/140‑1 (MQ=255)
                                                                                                aCTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGt                                        <  1:858989/140‑1 (MQ=255)
                                                                                                  tGCCGGAGATAATCGAGATCGGCGGGGGGTCGGATGCCCCCCTTTTTCAATAATATTTTCGCCCCCCGCTGGAGCCAGCGCCGGCCCCCCCCCCCCCCCCGCGATGCAACCTTGGTATCGCCGTTGTTCGCCCTCAGTAg                                      >  2:126322/1‑140 (MQ=255)
                                                                                                    ccGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGa                                    <  1:163519/140‑1 (MQ=255)
                                                                                                            aaTCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTa                            <  1:126322/140‑1 (MQ=255)
                                                                                                            aaTCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTa                            <  1:273139/140‑1 (MQ=255)
                                                                                                                            ggTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGtt            <  2:767600/140‑1 (MQ=255)
                                                                                                                              ttCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCa          <  1:503010/140‑1 (MQ=255)
                                                                                                                               tCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAg         <  1:84359/140‑1 (MQ=255)
                                                                                                                                      cccccTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTaa  >  2:225932/1‑140 (MQ=255)
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GCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAA  >  NC_000913/646621‑646894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑