Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,401,987	G→T	I22I (ATC→ATA)	nuoE ←	NADH:quinone oxidoreductase subunit E

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,401,987	0	G	T	100.0%	243.6 / NA	69	I22I (ATC→ATA)	nuoE	NADH:quinone oxidoreductase subunit E
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (31/38);  total (31/38)

GCTTGCCGGAATACCCAGCACATCGGCGATCGCGTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGTGGTGCATCTCGTGCTCGATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATTCTCGTGCATAATTAGCGGTCCACATCTGACATAACAAAATCGATACTGCCCAGATAAACAATCAGGTCAGACACCAGG  >  NC_000913/2401852‑2402121                                                                                                                                        |                                                                                                                                       gCTTGCCGGAATACCCAGCACATCGGCGATCGCGTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGTGGTGCATCTCGTGCTCTATCg                                                                                                                                    >  2:186574/1‑140 (MQ=255) gCTTGCCGGAATACCCAGCACATCGGCGATCGCGTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGTGGTGCATCTCGTGCTCTATCg                                                                                                                                    >  1:372888/1‑140 (MQ=255)   ttGCCGGAATACCCAGCACATCGGCGATCGCGTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGTGGTGCATCTCGTGCTCTATCGCt                                                                                                                                  <  2:372888/140‑1 (MQ=255)    tGCCGGAATACCCAGCACATCGGCGATCGCGTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGTGGTGCATCTCGTGCTCTATCGCtt                                                                                                                                 >  2:819488/1‑140 (MQ=255)        ggAATACCCAGCACATCGGCGATCGCGTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGTGGTGCATCTCGTGCTCTATCGCTTCACg                                                                                                                             <  2:583204/140‑1 (MQ=255)          aaTACCCAGCACATCGGCGATCGCGTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGTGGTGCATCTCGTGCTCTATCGCTTCACGct                                                                                                                           <  2:362338/140‑1 (MQ=255)          aaTACCCAGCACATCGGCGATCGCGTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGTGGTGCATCTCGTGCTCTATCGCTTCACGct                                                                                                                           <  2:506376/140‑1 (MQ=255)          aaTACCCAGCACATCGGCGATCGCGTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGTGGTGCATCTCGTGCTCTATCGCTTCACGct                                                                                                                           >  2:693506/1‑140 (MQ=255)             aCCCAGCACATCGGCGATCGCGTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGTGGTGCATCTCGTGCTCTATCGCTTCACGCTCTg                                                                                                                        <  1:283363/140‑1 (MQ=255)                  gCACATCGGCGATCGCGTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGTGGTGCATCTCGTGCTCTATCGCTTCACGCTCTGCCGCa                                                                                                                   <  1:243985/140‑1 (MQ=255)                    acaTCGGCGATCGCGTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGTGGTGCATCTCGTGCTCTATCGCTTCACGCTCTGCCGCACt                                                                                                                 <  1:13172/140‑1 (MQ=255)                    acaTCGGCGATCGCGTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGTGGTGCATCTCGTGCTCTATCGCTTCACGCTCTGCCGCACt                                                                                                                 <  1:412045/140‑1 (MQ=255)                    acaTCGGCGATCGCGTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGTGGTGCATCTCGTGCTCTATCGCTTCACGCTCTGCCGCACt                                                                                                                 <  1:595479/140‑1 (MQ=255)                      aTCGGCGATCGCGTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATTGACGCCGCACGCGGGTCTTCGTAGTGGTGCATCTCGTGCTCTATCGCTTCACGCTCTGCCGCACTCa                                                                                                               >  2:22682/1‑140 (MQ=255)                      aTCGGCGATCGCGTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGTGGTGCATCTCGTGCTCTATCGCTTCACGCTCTGCCGCACTCa                                                                                                               <  1:332479/140‑1 (MQ=255)                            gATCGCGTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGTGGTGCATCTCGTGCTCTATCGCTTCACGCTCTGCCGCACTCAGCTCaa                                                                                                         <  2:50311/140‑1 (MQ=255)                               cgcgTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGTGGTGCATCTCGTGCTCTATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAg                                                                                                      >  1:201662/1‑140 (MQ=255)                                 cgTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGTGGTGCATCTCGTGCTCTATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGcc                                                                                                    <  2:95/140‑1 (MQ=255)                                 cgTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGTGGTGCATCTCGTGCTCTATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGcc                                                                                                    <  2:577560/140‑1 (MQ=255)                                 cgTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGTGGTGCATCTCGTGCTCTATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGcc                                                                                                    >  1:529088/1‑140 (MQ=255)                                  gTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGTGGTGCATCTCGTGCTCTATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCt                                                                                                   <  2:782087/140‑1 (MQ=255)                                            ccATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGTGGTGCATCTCGTGCTCTATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTgg                                                                                         <  1:819488/140‑1 (MQ=255)                                               tCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGTGGTGCATCTCGTGCTCTATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGgttg                                                                                      <  2:125212/140‑1 (MQ=255)                                                ccGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGTGGTGCATCTCGTGCTCTATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGgttgt                                                                                     >  1:67313/1‑140 (MQ=255)                                                ccGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGTGGTGCATCTCGTGCTCTATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGgttgt                                                                                     >  1:217546/1‑140 (MQ=255)                                                   gCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGTGGTGCATCTCGTGCTCTATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGa                                                                                  <  1:186574/140‑1 (MQ=255)                                                   gCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGTGGTGCATCTCGTGCTCTATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGa                                                                                  <  1:693506/140‑1 (MQ=255)                                                    cACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGTGGTGCATCTCGTGCTCTATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTgg                                                                                         <  1:50100/132‑1 (MQ=255)                                                    cACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGTGGTGCATCTCGTGCTCTATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTgg                                                                                         >  2:50100/1‑132 (MQ=255)                                                     aCCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGTGGTGCATCTCGTGCTCTATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGAtt                                                                                >  1:769357/1‑140 (MQ=255)                                                      cccAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGTGGTGCATCTCGTGCTCTATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATtc                                                                               <  2:217546/140‑1 (MQ=255)                                                                ctgctTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGTGGTGCATCTCGTGCTCTATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATTCTCGTGCATaa                                                                     >  1:432147/1‑140 (MQ=255)                                                                    ttCTGACCGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGTGGTGCATCTCGTGCTCTATCGCTTCACACTCTGCCGCTCTCAGCTCAAACGCCTCGGTCTGTGGTTGTTGATTCTCCGGCCTAAttcg                                                                 <  2:698418/140‑3 (MQ=255)                                                                    ttCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGTGGTGCATCTCGTGCTCTATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATTCTCGTGCATAATTAg                                                                 <  2:652970/140‑1 (MQ=255)                                                                    ttCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGTGGTGCATCTCGTGCTCTATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATTCTCGTGCATAATTAg                                                                 <  2:201662/140‑1 (MQ=255)                                                                        gAACGATTTTCAGCGCTTCAATTGACGCCGCACGCGGGTCTTCGTAGTGGTGCATCTCGTGCTCTATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATTCTCGTGCATAATTAGCGGt                                                             <  1:22682/140‑1 (MQ=255)                                                                         aaCGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGTGGTGCATCTCGTGCTCTATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATTCTCGTGCATAATTAGCGGTc                                                            <  2:67313/140‑1 (MQ=255)                                                                           cGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGTGGTGCATCTCGTGCTCTATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATTCTCGTGCATAATTAg                                                                 >  2:642561/1‑133 (MQ=255)                                                                           cGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGTGGTGCATCTCGTGCTCTATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATTCTCGTGCATAATTAg                                                                 <  1:642561/133‑1 (MQ=255)                                                                           cGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGTGGTGCATCTCGTGCTCTATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATTCTCGTGCATAATTAGCGGTCca                                                          >  1:831218/1‑140 (MQ=255)                                                                             aTTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGTGGTGCATCTCGTGCTCTATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATTCTCGTGCATAATTAGCGGTCcaca                                                        >  2:448900/1‑140 (MQ=255)                                                                             aTTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGTGGTGCATCTCGTGCTCTATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATTCTCGTGCATAATTAGCGGTCcaca                                                        <  2:432147/140‑1 (MQ=255)                                                                              ttttCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGTGGTGCATCTCGTGCTCTATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATTCTCGTGCATAATTAGCGGTCCACAt                                                       >  2:686240/1‑140 (MQ=255)                                                                              ttttCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGTGGTGCATCTCGTGCTCTATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATTCTCGTGCATAATTAGCGGTCCACAt                                                       >  2:178147/1‑140 (MQ=255)                                                                                ttCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGTGGTGCATCTCGTGCTCTATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATTCTCGTGCATAATTAGCGGTCCACAt                                                       <  2:192718/138‑1 (MQ=255)                                                                                ttCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGTGGTGCATCTCGTGCTCTATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATTCTCGTGCATAATTAGCGGTCCACAt                                                       >  1:192718/1‑138 (MQ=255)                                                                                  cAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGTGGTGCATCTCGTGCTCTATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATTCTCGTGCATAATTAGCGGTCCACATCTGa                                                   <  2:831218/140‑1 (MQ=255)                                                                                    gcgcTTCAATGGACGCCGCACGCGGGTCTTCGTAGTGGTGCATCTCGTGCTCTATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATTCTCGTGCATAATTAGCGGTCcaca                                                        >  2:249574/1‑133 (MQ=255)                                                                                    gcgcTTCAATGGACGCCGCACGCGGGTCTTCGTAGTGGTGCATCTCGTGCTCTATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATTCTCGTGCATAATTAGCGGTCcaca                                                        <  1:249574/133‑1 (MQ=255)                                                                                        ttCAATGGACGCCGCACGCGGGTCTTCGTAGTGGTGCATCTCGTGCTCTATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATTCTCGTGCATAATTAGCGGTCCACATCTGACATAAc                                             <  2:760470/140‑1 (MQ=255)                                                                                        ttCAATGGACGCCGCACGCGGGTCTTCGTAGTGGTGCATCTCGTGCTCTATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATTCTCGTGCATAATTAGCGGTCCACATCTGACATAAc                                             <  1:448900/140‑1 (MQ=255)                                                                                         tCAATGGACGCCGCACGCGGGTCTTCGTAGTGGTGCATCTCGTGCTCTATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATTCTCGTGCATAATTAGCGGTCCACATCTGACATAACa                                            >  1:393608/1‑140 (MQ=255)                                                                                             tGGACGCCGCACGCGGGTCTTCGTAGTGGTGCATCTCGTGCTCTATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATTCTCGTGCATAATTAGCGGTCCACATCTGACATAACAAAAt                                        <  2:814626/140‑1 (MQ=255)                                                                                                aCGCCGCACGCGGGTCTTCGTAGTGGTGCATCTCGTGCTCTATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATTCTCGTGCATAATTAGCGGTCCACATCTGACATAACAAAATCGa                                     >  2:598050/1‑140 (MQ=255)                                                                                                  gccgcACGCGGGTCTTCGTAGTGGTGCATCTCGTGCTCTATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATTCTCGTGCATAATTAGCGGTCCACATCTGACATAACAAAATCGATa                                   <  2:529088/140‑1 (MQ=255)                                                                                                  gccgcACGCGGGTCTTCGTAGTGGTGCATCTCGTGCTCTATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATTCTCGTGCATAATTAGCGGTCCACATCTGACATAACAAAATCGATa                                   >  1:812635/1‑140 (MQ=255)                                                                                                    cgcACGCGGGTCTTCGTAGTGGTGCATCTCGTGCTCTATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATTCTCGTGCATAATTAGCGGTCCACATCTGACATAACAAAATCGATACt                                 >  1:869890/1‑140 (MQ=255)                                                                                                       aCGCGGGTCTTCGTAGTGGTGCATCTCGTGCTCTATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATTCTCGTGCATAATTAGCGGTCCACATCTGACATAACAAAATCGATACTGcc                              <  1:178147/140‑1 (MQ=255)                                                                                                             gTCTTCGTAGTGGTGCATCTCGTGCTCTATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATTCTCGTGCATAATTAGCGGTCCACATCTGACATAACAAAATCGATACTGCCCAGATa                        <  2:812635/140‑1 (MQ=255)                                                                                                                ttCGTAGTGGTGCATCTCGTGCTCTATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATTCTCGTGCATAATTAGCGGTCCACATCTGACATAACAAAATCGATACTGCCCAGATAAAc                     >  2:26342/1‑140 (MQ=255)                                                                                                                       tggtgCATCTCGTGCTCTATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATTCTCGTGCATAATTAGCGGTCCACATCTGACATAACAAAATCGATACTGCCCAGATAAACAATCAgg              >  1:856148/1‑140 (MQ=255)                                                                                                                         gtgCATCTCGTGCTCTATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATTCTCGTGCATAATTAGCGGTCCACATCTGACATAACAAAATCGATACTGCCCAGATAAACAATCAGGTc            <  2:769357/140‑1 (MQ=255)                                                                                                                            cATCTCGTGCTCTATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATTCTCGTGCATAATTAGCGGTCCACATCTGACATAACAAAATCGATACTGCCCAGATAAACAATCAGGTCAGa         >  1:482142/1‑140 (MQ=255)                                                                                                                            cATCTCGTGCTCTATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATTCTCGTGCATAATTAGCGGTCCACATCTGACATAACAAAATCGATACTGCCCAGATAAACAATCAGGTCAGa         >  1:796960/1‑140 (MQ=255)                                                                                                                            cATCTCGTGCTCTATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATTCTCGTGCATAACTAGCGGTCCACATCTGACATAACAAAATCGATACTGCCCAGATAAACAATCAGGTCAGa         >  1:835630/1‑140 (MQ=255)                                                                                                                              tctcGTGCTCTATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATTCTCGTGCATAATTAGCGGTCCACATCTGACATAACAAAATCGATACTGCCCAGATAAACAATCAGGTCAGaca       >  1:373895/1‑140 (MQ=255)                                                                                                                              tctcGTGCTCTATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATTCTCGTGCATAATTAGCGGTCCACATCTGACATAACAAAATCGATACTGCCCAGATAAACAATCAGGTCAGaca       >  2:409468/1‑140 (MQ=255)                                                                                                                                 cGTGCTCTATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATTCTCGTGCATAATTAGCGGTCCACATCTGACATAACAAAATCGATACTGCCCAGATAAACAATCAGGTCAGACACCa    >  1:679613/1‑140 (MQ=255)                                                                                                                                   tGCTCTATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATTCTCGTGCATAATTAGCGGTCCACATCTGACATAACAAAATCGATACTGCCCAGATAAACAATCAGGTCAGACACCAgg  <  2:679613/140‑1 (MQ=255)                                                                                                                                   tGCTCTATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATTCTCGTGCATAATTAGCGGTCCACATCTGACATAACAAAATCGATACTGCCCAGATAAACAATCAGGTCAGACACCAgg  <  1:26342/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       GCTTGCCGGAATACCCAGCACATCGGCGATCGCGTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAGCGCTTCAATGGACGCCGCACGCGGGTCTTCGTAGTGGTGCATCTCGTGCTCGATCGCTTCACGCTCTGCCGCACTCAGCTCAAAAGCCTCGGTTTGTGGTTGTTGATTCTCGTGCATAATTAGCGGTCCACATCTGACATAACAAAATCGATACTGCCCAGATAAACAATCAGGTCAGACACCAGG  >  NC_000913/2401852‑2402121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑