Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,589,882	C→A	A466A (GCG→GCT)	btsT ←	pyruvate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,589,882	0	C	A	100.0%	169.5 / NA	49	A466A (GCG→GCT)	btsT	pyruvate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (25/24);  total (25/24)

TACCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGAACAGAATACCGAAGTGATACCAGAAGCCCATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGCGCGCCACCTGCACGGTTCAGGACAGAAGGCTCACCAATGTCTTTCGCGGTTTGCAGGATCTGCTCTGGCGAAATCACGAAGCCCCAGGAGCTGACGGTCGCTGCCGCGTGTGCGGTAACGTCTTTCAGCTGCGCC  >  NC_000913/4589755‑4590017                                                                                                                                |                                                                                                                                        tACCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGAACAGAATACCGAAGTGATACCAGAAGCCCATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGAGCGCCACCTGCa                                                                                                                             <  2:762562/140‑1 (MQ=255)      gCATCCAGCGCGGTCAGGATGAACAGGGCTTCGAACAGAATACCGAAGTGATACCAGAAGCCCATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGAGCGCCACCTGCACGGtt                                                                                                                        <  1:370108/140‑1 (MQ=255)      gCATCCAGCGCGGTCAGGATGAACAGGGCTTCGAACAGAATACCGAAGTGATACCAGAAGCCCATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGAGCGCCACCTGCACGGtt                                                                                                                        >  2:666043/1‑140 (MQ=255)              cgcgGTCAGGATGAACAGGGCTTCGAACAGAATACCGAAGTGATACCAGAAGCCCATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGAGCGCCACCTGCa                                                                                                                             >  1:168186/1‑127 (MQ=255)              cgcgGTCAGGATGAACAGGGCTTCGAACAGAATACCGAAGTGATACCAGAAGCCCATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGAGCGCCACCTGCa                                                                                                                             <  2:168186/127‑1 (MQ=255)               gcgGTCAGGATGAACAGGGCTTCGAACAGAATACCGAAGTGATACCAGAAGCCCATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGAGCGCCACCTGCACGGTTCAGGACAGa                                                                                                               <  2:678741/140‑1 (MQ=255)                     aGGATGAACAGGGCTTCGAACAGAATACCGAAGTGATACCAGAAGCCCATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGAGCGCCACCTGCACGGTTCAGGACAGAAGGCTc                                                                                                         >  2:104035/1‑140 (MQ=255)                             cAGGGCTTCGAACAGAATACCGAAGTGATACCAGAAGCCCATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGAGCGCCACCTGCACGGTTCAGGACAGAAGGCTCACCAATGt                                                                                                 >  1:607350/1‑140 (MQ=255)                             cAGGGCTTCGAACAGAATACCGAAGTGATACCAGAAGCCCATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGAGCGCCACCTGCACGGTTCAGGACAGAAGGCTCACCAATGt                                                                                                 >  2:620180/1‑140 (MQ=255)                               gggCTTCGAACAGAATACCGAAGTGATACCAGAAGCCCATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGAGCGCCACCTGCACGGTTCAGGACAGAAGGCTCACCAATGTCt                                                                                               >  1:703446/1‑140 (MQ=255)                                ggCTTCGAACAGAATACCGAAGTGATACCAGAAGCCCATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGAGCGCCACCTGCACGGTTCAGGACAGAAGGCTCACCAATGTCtt                                                                                              <  2:83106/140‑1 (MQ=255)                                          aGAATACCGAAGTGATACCAGAAGCCCATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGAGCGCCACCTGCACGGTTCAGGACAGAAGGCTCACCAATGTCTTTCGCGGTTTg                                                                                    <  1:130332/140‑1 (MQ=255)                                          aGAATACCGAAGTGATACCAGAAGCCCATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGAGCGCCACCTGCACGGTTCAGGACAGAAGGCTCACCAATGTCTTTCGCGGTTTg                                                                                    <  2:792221/140‑1 (MQ=255)                                            aaTACCGAAGTGATACCAGAAGCCCATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGAGCGCCACCTGCACGGTTCAGGACAGAAGGCTCACCAATGTCTTTcgc                                                                                          >  1:100752/1‑132 (MQ=255)                                            aaTACCGAAGTGATACCAGAAGCCCATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGAGCGCCACCTGCACGGTTCAGGACAGAAGGCTCACCAATGTCTTTcgc                                                                                          <  2:100752/132‑1 (MQ=255)                                             aTACCGAAGTGATACCAGAAGCCCATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGAGCGCCACCTGCACGGTTCAGGACAGAAGGCTCACCAATGTCTTTCGCGGTTTGCAg                                                                                 >  1:53308/1‑140 (MQ=255)                                                ccGAAGTGATACCAGAAGCCCATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGAGCGCCACCTGCACGGTTCAGGACAGAAGGCTCACCAATGTCTTTCGCGGTTTGCAGGAt                                                                              <  2:607350/140‑1 (MQ=255)                                                ccGAAGTGATACCAGAAGCCCATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGAGCGCCACCTGCACGGTTCAGGACAGAAGGCTCACCAATGTCTTTCGCGGTTTGCAGGAt                                                                              <  2:53308/140‑1 (MQ=255)                                                      tGATACCAGAAGCCCATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGAGCGCCACCTGCACGGTTCAGGACAGAAGGCTCACCAATGTCTTTCGCGGTTTGCAGGATCTGctc                                                                        >  2:18872/1‑140 (MQ=255)                                                      tGATACCAGAAGCCCATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGAGCGCCACCTGCACGGTTCAGGACAGAAGGCTCACCAATGTCTTTCGCGGTTTGCAGGATCTGctc                                                                        >  1:529775/1‑140 (MQ=255)                                                        aTACCAGAAGCCCATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGAGCGCCACCTGCACGGTTCAGGACAGAAGGCTCACCAATGTCTTTCGCGGTTTGCAGGATCTGCTCTg                                                                      >  1:524289/1‑140 (MQ=255)                                                               aaGCCCATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGAGCGCCACCTGCACGGTTCAGGACAGAAGGCTCACCAATGTCTTTCGCGGTTTGCAGGATCTGCTCTGGCGAAAt                                                               >  2:220212/1‑140 (MQ=255)                                                               aaGCCCATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGAGCGCCACCTGCACGGTTCAGGACAGAAGGCTCACCAATGTCTTTCGCGGTTTGCAGGATCTGCTCTGGCGAAAt                                                               >  2:86003/1‑140 (MQ=255)                                                                  cccATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGAGCGCCACCTGCACGGTTCAGGACAGAAGGCTCACCAATGTCTTTCGCGGTTTGCAGGATCTGCTCTGGCGAAATCAc                                                            <  2:703446/140‑1 (MQ=255)                                                                    cATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGAGCGCCACCTGCACGGTTCAGGACAGAAGGCTCACCAATGTCTTTCGCGGTTTGCAGGATCTGCTCTGGCGAAATCACGa                                                          <  2:529775/140‑1 (MQ=255)                                                                    cATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGAGCGCCACCTGCACGGTTCAGGACAGAAGGCTCACCAATGTCTTTCGCGGTTTGCAGGATCTGCTCTGGCGAAATCACGa                                                          <  1:220212/140‑1 (MQ=255)                                                                      tGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGAGCGCCACCTGCACGGTTCAGGACAGAAGGCTCACCAATGTCTTTCGCGGTTTGCAGGATCTGCTCTGGc                                                                    <  2:513292/128‑1 (MQ=255)                                                                      tGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGAGCGCCACCTGCACGGTTCAGGACAGAAGGCTCACCAATGTCTTTCGCGGTTTGCAGGATCTGCTCTGGc                                                                    >  1:513292/1‑128 (MQ=255)                                                                           gCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGAGCGCCACCTGCACGGTTCAGGACAGAAGGCTCACCAATGTCTTTCGCGGTTTGCAGGATCTGCTCTGGCGAAATCACGaa                                                         >  2:248171/1‑134 (MQ=255)                                                                           gCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGAGCGCCACCTGCACGGTTCAGGACAGAAGGCTCACCAATGTCTTTCGCGGTTTGCAGGATCTGCTCTGGCGAAATCACGaa                                                         <  1:248171/134‑1 (MQ=255)                                                                                cGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGAGCGCCACCTGCACGGTTCAGGACAGAAGGCTCACCAATGTCTTTCGCGGTTTGCAGGATCTGCTCTGGCGAAATCACGa                                                          >  1:743911/1‑128 (MQ=255)                                                                                cGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGAGCGCCACCTGCACGGTTCAGGACAGAAGGCTCACCAATGTCTTTCGCGGTTTGCAGGATCTGCTCTGGCGAAATCACGa                                                          <  2:743911/128‑1 (MQ=255)                                                                                cGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGAGCGCCACCTGCACGGTTCAGGACAGAAGGCTCACCAATGTCTTTCGCGGTTTGCAGGATCTGCTCTGGCGAAATCACGAAGCCCCAGGAGc                                              >  2:747844/1‑140 (MQ=255)                                                                                      caCTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGAGCGCCACCTGCACGGTTCAGGACAGAAGGCTCACCAATGTCTTTCGCGGTTTGCAGGATCTGCTCTGGCGAAATCACGAAGCCCCAGGAGCTGACgg                                        >  2:298276/1‑140 (MQ=255)                                                                                        cTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGAGCGCCACCTGCACGGTTCAGGACAGAAGGCTCACCAATGTCTTTCGCGGTTTGCAGGATCTGCTCTGGCGAAATCACGAAGCCCCAGGAGCTGACGGTc                                      >  2:262924/1‑140 (MQ=255)                                                                                            gtgGAACACGTGCGCGATACCTACCGCCAGCGTCGGAGCGCCACCTGCACGGTTCAGGACAGAAGGCTCACCAATGTCTTTCGCGGTTTGCAGGATCTGCTCTGGCGAAATCACGAAGCCCCAGGAGCTGACGGTCGCTg                                  >  1:813369/1‑140 (MQ=255)                                                                                              ggAACACGTGCGCGATACCTACCGCCAGCGTCGGAGCGCCACCTGCACGGTTCAGGACAGAAGGCTCACCAATGTCTTTCGCGGTTTGCAGGATCTGCTCTGGCGAAATCACGAAGCCCCAGGAGCTGACGGTCGCTGcc                                <  2:813369/140‑1 (MQ=255)                                                                                                 acacGTGCGCGATACCTACCGCCAGCGTCGGAGCGCCACCTGCACGGTTCAGGACAGAAGGCTCACCAATGTCTTTCGCGGTTTGCAGGATCTGCTCTGGCGAAATCACGAAGCCCCAGGAGCTGACGGTCGCTGcc                                <  1:593079/137‑1 (MQ=255)                                                                                                 acacGTGCGCGATACCTACCGCCAGCGTCGGAGCGCCACCTGCACGGTTCAGGACAGAAGGCTCACCAATGTCTTTCGCGGTTTGCAGGATCTGCTCTGGCGAAATCACGAAGCCCCAGGAGCTGACGGTCGCTGcc                                >  2:593079/1‑137 (MQ=255)                                                                                                  cacGTGCGCGATACCTACCGCCAGCGTCGGAGCGCCACCTGCACGGTTCAGGACAGAAGGCTCACCAATGTCTTTCGCGGTTTGCAGGATCTGCTCTGGCGAAATCACGAAGCCCCAGGAGCTGACGGTCGCTGCCGCgt                            >  2:730540/1‑140 (MQ=255)                                                                                                     gTGCGCGATACCTACCGCCAGCGTCGGAGCGCCACCTGCACGGTTCAGGACAGAAGGCTCACCAATGTCTTTCGCGGTTTGCAGGATCTGCTCTGGCGAAATCACGACGCCCCAGGAGCTGACGGTCGCTGCCGCGtgtg                         >  1:622246/1‑140 (MQ=255)                                                                                                           gATACCTACCGCCAGCGTCGGAGCGCCACCTGCACGGTTCAGGACAGAAGGCTCACCAATGTCTTTCGCGGTTTGCAGGATCTGCTCTGGCGAAATCACGAAGCCCCAGGAGCTGACGGTCGCTGCCGCGTGTGCGGTaa                   <  1:86003/140‑1 (MQ=255)                                                                                                            aTACCTACCGCCAGCGTCGGAGCGCCACCTGCACGGTTCAGGACAGAAGGCTCACCAATGTCTTTCGCGGTTTGCAGGATCTGCTCTGGCGAAATCACGAAGCCCCAGGAGCTGACGGTCGCTGCCGCGTGTGCGGTAAc                  >  1:641950/1‑140 (MQ=255)                                                                                                                  accGCCAGCGTCGGAGCGCCACCTGCACGGTTCAGGACAGAAGGCTCACCAATGTCTTTCGCGGTTTGCAGGATCTGCTCTGGCGAAATCACGAAGCCCCAGGAGCTGACGGTCGCTGCCGCGTGTGCGGTAACGTCttt            <  2:641950/140‑1 (MQ=255)                                                                                                                     gCCAGCGTCGGAGCGCCACCTGCACGGTTCAGGACAGAAGGCTCACCAATGTCTTTCGCGGTTTGCAGGATCTGCTCTGGCGAAATCACGAAGCCCCAGGAGCTGACGGTCGCTGCCGCGTGTGCGGTAACGTCTTTCAg         <  1:18872/140‑1 (MQ=255)                                                                                                                       cAGCGTCGGAGCGCCACCTGCACGGTTCAGGACAGAAGGCTCACCAATGTCTTTCGCGGTTTGCAGGATCTGCTCTGGCGAAATCACGAAGCCCCAGGAGCTGACGGTCGCTGCCGCGTGTGCGGTAACGTCTTTCAGCt       <  1:104035/140‑1 (MQ=255)                                                                                                                         gCGTCGGAGCGCCACCTGCACGGTTCAGGACAGAAGGCTCACCAATGTCTTTCGCGGTTTGCAGGATCTGCTCTGGCGAAATCACGAAGCCCCAGGAGCTGACGGTCGCTGCCGCGTGTGCGGTAACGTCTTTCAGCTgc     >  2:825575/1‑140 (MQ=255)                                                                                                                          cGTCGGAGCGCCACCTGCACGGTTCAGGACAGAAGGCTCACCAATGTCTTTCGCGGTTTGCAGGATCTGCTCTGGCGAAATCACGAAGCCCCAGGAGCTGACGGTCGCTGCCGCGTGTGCGGTAACGTCTTTCAGCTgcg    <  1:620180/140‑1 (MQ=255)                                                                                                                            tCGGAGCGCCACCTGCACGGTTCAGGACAGAAGGCTCACCAATGTCTTTCGCGGTTTGCAGGATCTGCTCTGGCGAAATCACGAAGCCCCAGGAGCTGACGGTCGCTGCCGCGTGTGCGGTAACGTCTTTCAGCTGCGcc  <  2:842449/140‑1 (MQ=255)                                                                                                                                |                                                                                                                                        TACCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGAACAGAATACCGAAGTGATACCAGAAGCCCATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGCGCGCCACCTGCACGGTTCAGGACAGAAGGCTCACCAATGTCTTTCGCGGTTTGCAGGATCTGCTCTGGCGAAATCACGAAGCCCCAGGAGCTGACGGTCGCTGCCGCGTGTGCGGTAACGTCTTTCAGCTGCGCC  >  NC_000913/4589755‑4590017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑