Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,183,287	C→A	A112S (GCG→TCG)	potC ←	spermidine preferential ABC transporter membrane subunit PotC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,183,287	0	C	A	100.0%	199.9 / NA	58	A112S (GCG→TCG)	potC	spermidine preferential ABC transporter membrane subunit PotC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (37/21);  total (37/21)

TCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATACCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGCGATAACGGTACAGCGCAACTGCCGTCAGTGAACCGATAAGCGTAGCAAACGTCGCCGAAAACACCGC  >  NC_000913/1183150‑1183422                                                                                                                                          |                                                                                                                                        tCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATACCCAGCAGCATAAACAGCACCAGCAGCGAAATCGACa                                                                                                                                       >  2:785008/1‑140 (MQ=255)   gCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATACCCAGCAGCATAAACAGCACCAGCAGCGAAATCGACATc                                                                                                                                     >  1:123705/1‑140 (MQ=255)     aCGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATACCCAGCAGCATAAACAGCACCAGCAGCGAAATCGACATCAc                                                                                                                                   >  1:577378/1‑140 (MQ=255)                 ttCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATACCCAGCAGCATAAACAGCACCAGCAGCGAAATCGACATCACGATATCTGGTGa                                                                                                                       >  1:516185/1‑140 (MQ=255)                   cAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATACCCAGCAGCATAAACAGCACCAGCAGCGAAATCGACATCACGATATCTGGTGAca                                                                                                                     >  2:728550/1‑140 (MQ=255)                        gcgAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATACCCAGCAGCATAAACAGCACCAGCAGCGAAATCGACATCACGATATCTGGTGACatcatc                                                                                                                <  1:841607/140‑1 (MQ=255)                                acCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATACCCAGCAGCATAAACAGCACCAGCAGCGAAATCGACATCACGATATCTGGTGACATCATCACCACaaa                                                                                                        >  1:13073/1‑140 (MQ=255)                                 ccGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATACCCAGCAGCATAAACAGCACCAGCAGCGAAATCGACATCACGATATCTGGTGACATCATCACCACAAAc                                                                                                       >  1:244685/1‑140 (MQ=255)                                      accaccacAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATACCCAGCAGCATAAACAGCACCAGCAGCGAAATCGACATCACGATATCTGGTGACAtcatca                                                                                                               <  2:670472/127‑1 (MQ=255)                                      accaccacAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATACCCAGCAGCATAAACAGCACCAGCAGCGAAATCGACATCACGATATCTGGTGACAtcatca                                                                                                               >  1:670472/1‑127 (MQ=255)                                           cacaAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATACCCAGCAGCATAAACAGCACCAGCAGCGAAATCGACATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGc                                                                                             <  2:123705/140‑1 (MQ=255)                                                  ggcaggcaGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATACCCAGCAGCATAAACAGCACCAGCAGCGAAATCGACATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTaacgaa                                                                                      >  1:106227/1‑140 (MQ=255)                                                  ggcaggcaGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATACCCAGCAGCATAAACAGCACCAGCAGCGAAATCGACATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTaacgaa                                                                                      >  2:90356/1‑140 (MQ=255)                                                   gcaggcagAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATACCCAGCAGCATAAACAGCACCAGCAGCGAAATCGACATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTaacgaac                                                                                     <  1:728550/140‑1 (MQ=255)                                                    caggcagAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATACCCAGCAGCATAAACAGCACCAGCAGCGAAATCGACATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTaacgaacg                                                                                    >  2:566356/1‑140 (MQ=255)                                                     aggcagAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATACCCAGCAGCATAAACAGCACCAGCAGCGAAATCGACATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACgg                                                                                   >  1:458128/1‑140 (MQ=255)                                                       gcagAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATACCCAGCAGCATAAACAGCACCAGCAGCGAAATCGACATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCt                                                                                 >  1:630904/1‑140 (MQ=255)                                                              gTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATACCCAGCAGCATAAACAGCACCAGCAGCGAAATCGACATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACg                                                                          <  2:805036/140‑1 (MQ=255)                                                                gATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATACCCAGCAGCATAAACAGCACCAGCAGCGAAATCGACATCACGTTATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGaa                                                                        <  1:785008/140‑1 (MQ=255)                                                                 atatGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATACCCAGCAGCATAAACAGCACCAGCAGCGAAATCGACATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGaaa                                                                       >  2:127671/1‑140 (MQ=255)                                                                 atatGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATACCCAGCAGCATAAACAGCACCAGCAGCGAAATCGACATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGaaa                                                                       >  2:693065/1‑140 (MQ=255)                                                                  tatGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATACCCAGCAGCATAAACAGCACCAGCAGCGAAATCGACATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAg                                                                      >  1:1038/1‑140 (MQ=255)                                                                   atGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATACCCAGCAGCATAAACAGCACCAGCAGCGAAATCGACATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGc                                                                     <  2:13073/140‑1 (MQ=255)                                                                   atGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATACCCAGCAGCATAAACAGCACCAGCAGCGAAATCGACATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGc                                                                     <  1:880779/140‑1 (MQ=255)                                                                     gCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATACCCAGCAGCATAAACAGCACCAGCAGCGAAATTGACATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGaaa                                                                       <  2:298717/136‑1 (MQ=255)                                                                     gCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATACCCAGCAGCATAAACAGCACCAGCAGCGAAATTGACATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGaaa                                                                       >  1:298717/1‑136 (MQ=255)                                                                     gCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATACCCAGCAGCATAAACAGCACCAGCAGCGAAATCGACATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGCGa                                                                   >  2:883468/1‑140 (MQ=255)                                                                     gCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATACCCAGCAGCATAAACAGCACCAGCAGCGAAATCGACATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGCGa                                                                   <  2:106227/140‑1 (MQ=255)                                                                        agaACAGCAGCGACCAGAAGCCAAGCTGAATACCCAGCAGCATAAACAGCACCAGCAGCGAAATCGACATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGCGATaa                                                                <  2:516185/140‑1 (MQ=255)                                                                         gaACAGCAGCGACCAGAAGCCAAGCTGAATACCCAGCAGCATAAACAGCACCAGCAGCGAAATCGACATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGc                                                                     <  1:137213/134‑1 (MQ=255)                                                                         gaACAGCAGCGACCAGAAGCCAAGCTGAATACCCAGCAGCATAAACAGCACCAGCAGCGAAATCGACATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGc                                                                     >  2:137213/1‑134 (MQ=255)                                                                          aaCAGCAGCGACCAGAAGCCAAGCTGAATACCCAGCAGCATAAACAGCACCAGCAGCGAAATCGACATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGCGATAACg                                                              >  1:693066/1‑140 (MQ=255)                                                                           aCAGCAGCGACCAGAAGCCAAGCTGAATACCCAGCAGCATAAACAGCACCAGCAGCGAAATCGACATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGCGATAACgg                                                             >  2:268706/1‑140 (MQ=255)                                                                               cagcGACCAGAAGCCAAGCTGAATACCCAGCAGCATAAACAGCACCAGCAGCGAAATCGACATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGCGATAACGGTACa                                                         >  1:369574/1‑140 (MQ=255)                                                                               cagcGACCAGAAGCCAAGCTGAATACCCAGCAGCATAAACAGCACCAGCAGCGAAATCGACATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGCGATAACGGTACa                                                         >  1:24373/1‑140 (MQ=255)                                                                               cagcGACCAGAAGCCAAGCTGAATACCCAGCAGCATAAACAGCACCAGCAGCGAAATCGACATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGCGATAACGGTACa                                                         >  2:33381/1‑140 (MQ=255)                                                                               cagcGACCAGAAGCCAAGCTGAATACCCAGCAGCATAAACAGCACCAGCAGCGAAATCGACATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGCGATAACGGTACa                                                         >  2:178395/1‑140 (MQ=255)                                                                                    aCCAGAAGCCAAGCTGAATACCCAGCAGCATAAACAGCACCAGCAGCGAAATCGACATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGCGATAACGGTACAGCGCa                                                    >  2:706274/1‑140 (MQ=255)                                                                                    aCCAGAAGCCAAGCTGAATACCCAGCAGCATAAACAGCACCAGCAGCGAAATCGACATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGCGATAACGGTACAGCGCa                                                    >  2:824118/1‑140 (MQ=255)                                                                                      cAGAAGCCAAGCTGAATACCCAGCAGCATAAACAGCACCAGCAGCGAAATCGACATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGCGATAACGGTACAGCGCAAc                                                  <  2:24373/140‑1 (MQ=255)                                                                                          aGCCAAGCTGAATACCCAGCAGCATAAACAGCACCAGCAGCGAAATCGACATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGCGATAACGGTACAGCGCAACTGcc                                              <  1:178395/140‑1 (MQ=255)                                                                                                 cTGAATACCCAGCAGCATAAACAGCACCAGCAGCGAAATCGACATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGCGATAACGGTACAGCGCAACTGCCGTCAGTg                                       <  1:127671/140‑1 (MQ=255)                                                                                                 cTGAATACCCAGCAGCATAAACAGCACCAGCAGCGAAATCGACATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGCGATAACGGTACAGCGCAACTGCCGTCAGTg                                       <  1:693065/140‑1 (MQ=255)                                                                                                         ccAGCAGCATAAACAGCACCAGCAGCGAAATCGACATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGCGATAACGGTACAGCGCAACTGCCGTCAGTGAACCGATa                               >  1:789267/1‑140 (MQ=255)                                                                                                           agcagcATAAACAGCACCAGCAGCGAAATCGACATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGCGATAACGGTACAGCGCAACTGCCGTCAGt                                        >  2:865878/1‑129 (MQ=255)                                                                                                           agcagcATAAACAGCACCAGCAGCGAAATCGACATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGCGATAACGGTACAGCGCAACTGCCGTCAGt                                        <  1:865878/129‑1 (MQ=255)                                                                                                              agcaTAAACAGCACCAGCAGCGAAATCGACATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGCGATAACGGTACAGCGCAACTGCCGTCAGTGAACCGATAAGCGt                          >  1:107753/1‑140 (MQ=255)                                                                                                                          aCCAGCAGCGAAATCGACATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGCGATAACGGTACAGCGCAACTGCCGTCAGTGAACCGATAAGCGTAGCAAACGTCGc              >  2:785987/1‑140 (MQ=255)                                                                                                                           ccAGCAGCGAAATCGACATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGCGATAACGGTACAGCGCAACTGCCGTCAGTGAACCGATAAGCGTAGCAAACGTCGcc             >  1:735569/1‑140 (MQ=255)                                                                                                                                agcGAAATCGACATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGCGATAACGGTACAGCGCAACTGCCGTCAGTGAACCGATAAGCGTAGCAAACGTCGCCGaaaa        >  1:157094/1‑140 (MQ=255)                                                                                                                                 gcGAAATCGACATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGCGATAACGGTACAGCGCAACTGCCGTCAGTGAACCGATAAGCGTAGCAAACGTCGCCGAAAac       >  2:640760/1‑140 (MQ=255)                                                                                                                                 gcGAAATCGACATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGCGATAACGGTACAGCGCAACTGCCGTCAGTGAACCGATAAGCGTAGCAAACGTCGCCGAAAac       >  2:32362/1‑140 (MQ=255)                                                                                                                                    aaaTCGACATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGCGATAACGGTACAGCGCAACTGCCGTCAGTGAACCGATAAGCGTAGCAAACGTCGCCGAAAACAcc    >  1:68098/1‑140 (MQ=255)                                                                                                                                    aaaTCGACATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGCGATAACGGTACAGCGCAACTGCCGTCAGTGAACCGATAAGCGTAGCAAACGTCGCCGAAAACAcc    <  1:883468/140‑1 (MQ=255)                                                                                                                                      aTCGACATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGCGATAACGGTACAGCGCAACTGCCGTCAGTGAACCGATAAGCGTAGCAAACGTCGCCGAAAACAccac  >  1:676205/1‑138 (MQ=255)                                                                                                                                      aTCGACATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGCGATAACGGTACAGCGCAACTGCCGTCAGTGAACCGATAAGCGTAGCAAACGTCGCCGAAAACACCGc  <  2:1038/140‑1 (MQ=255)                                                                                                                                      aTCGACATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGCGATAACGGTACAGCGCAACTGCCGTCAGTGAACCGATAAGCGTAGCAAACGTCGCCGAAAACACCGc  <  2:305485/140‑1 (MQ=255)                                                                                                                                      aTCGACATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGCGATAACGGTACAGCGCAACTGCCGTCAGTGAACCGATAAGCGTAGCAAACGTCGCCGAAAACACCGc  <  2:157094/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        TCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATACCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGCGATAACGGTACAGCGCAACTGCCGTCAGTGAACCGATAAGCGTAGCAAACGTCGCCGAAAACACCGC  >  NC_000913/1183150‑1183422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑