Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,187,106 C→A intergenic (+36/+13) pepT → / ← roxA peptidase T/ribosomal protein‑arginine oxygenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,187,1060CA95.2% 136.5 / 0.6 42intergenic (+36/+13)pepT/roxApeptidase T/ribosomal protein‑arginine oxygenase
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (22/18);  total (23/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

CATGGTAAGCATGAGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTGGCGAGTGCATC  >  NC_000913/1186978‑1187241
                                                                                                                                |                                                                                                                                       
cATGGTAAGCATGAGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCTGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGATTAAATCCaaa                                                                                                                              >  2:725481/1‑140 (MQ=255)
cATGGTAAGCATGAGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGATTAAATCCaaa                                                                                                                              >  2:19107/1‑140 (MQ=255)
         cATGAGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGATTAAATCCAAACTTACCCtt                                                                                                                     >  1:144342/1‑140 (MQ=255)
             aGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCgaa                                                                                                                 >  2:810196/1‑140 (MQ=255)
             aGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGATTAAATCCAAACTTACCCTTCgaa                                                                                                                 >  2:38784/1‑140 (MQ=255)
             aGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGATTAAATCCAAACTTACCCTTCgaa                                                                                                                 >  1:410433/1‑140 (MQ=255)
                 tgtgACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGATTAAATCCAAACTTACCCTTCGAAGAAc                                                                                                             >  2:305176/1‑140 (MQ=255)
                   tgACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGATTAAATCCAAACTTACCCTTCGAAGAACCa                                                                                                           <  1:841678/140‑1 (MQ=255)
                       tctGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGATTAAATCCAAACTTACCCTTCGAAGAACCAATAc                                                                                                       >  2:63978/1‑140 (MQ=255)
                        ctGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGATTAAATCCAAACTTACCCTTCGAAGAACCAATAcc                                                                                                      <  2:410433/140‑1 (MQ=255)
                                tATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGATTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTg                                                                                              >  1:447041/1‑140 (MQ=255)
                                  tGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGATTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGAc                                                                                            >  2:697095/1‑140 (MQ=255)
                                   ggAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGATTAAATCCAAACTTACCCTTCGAAGAACCAATAcc                                                                                                      <  1:869679/129‑1 (MQ=255)
                                   ggAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGATTAAATCCAAACTTACCCTTCGAAGAACCAATAcc                                                                                                      >  2:869679/1‑129 (MQ=255)
                                     aaaaaGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGATTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAg                                                                                         >  2:543251/1‑140 (MQ=255)
                                          gCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGATTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGcgccg                                                                                    >  1:395648/1‑140 (MQ=255)
                                               gCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGATTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGATCAGCGCCGCGAGc                                                                               <  1:296340/140‑1 (MQ=255)
                                               gCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGATTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGc                                                                               <  1:132735/140‑1 (MQ=255)
                                                   gTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGATTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATcg                                                                           <  2:447041/140‑1 (MQ=255)
                                                     gATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGATTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATcgcg                                                                         <  2:585303/140‑1 (MQ=255)
                                                     gATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGATTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATcgcg                                                                         <  2:144342/140‑1 (MQ=255)
                                                        cGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGATTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATcgcg                                                                         <  1:135764/137‑1 (MQ=255)
                                                        cGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGATTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATcgcg                                                                         >  2:135764/1‑137 (MQ=255)
                                                           ccGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGATTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAAt                                                                   >  2:534750/1‑140 (MQ=255)
                                                            cGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGATTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATg                                                                  <  1:63978/140‑1 (MQ=255)
                                                            cGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGATTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATg                                                                  <  2:871764/140‑1 (MQ=255)
                                                             gTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGATTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGa                                                                 >  1:653805/1‑140 (MQ=255)
                                                             gTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGATTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGa                                                                 >  1:590820/1‑140 (MQ=255)
                                                                      aGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGATTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTc                                                        >  1:798610/1‑140 (MQ=255)
                                                                                              cGAAAAGGGATGCGGCATGTGATGCCGCATCCGGATTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCa                                           >  1:870967/1‑129 (MQ=255)
                                                                                              cGAAAAGGGATGCGGCATGTGATGCCGCATCCGGATTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCa                                           <  2:870967/129‑1 (MQ=255)
                                                                                                   aGGGATGCGGCATGTGATGCCGCATCCGGATTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCa                           <  2:798610/140‑1 (MQ=255)
                                                                                                        tGCGGCATGTGATGCCGCATCCGGATTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCa                      >  2:759889/1‑140 (MQ=255)
                                                                                                         gCGGCATGTGATGCCGCATCCGGATTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGTGCaa                     <  1:697095/140‑1 (MQ=255)
                                                                                                             cATGTGATGCCGCATCCGGATTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGt                             >  1:342669/1‑128 (MQ=255)
                                                                                                             cATGTGATGCCGCATCCGGATTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGt                             <  2:342669/128‑1 (MQ=255)
                                                                                                                  gATGCCGCATCCGGATTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTgg            <  2:653805/140‑1 (MQ=255)
                                                                                                                       cGCATCCGGATTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCaa                     <  2:571409/126‑1 (MQ=255)
                                                                                                                       cGCATCCGGATTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCaa                     >  1:571409/1‑126 (MQ=255)
                                                                                                                       cGCATCCGGATTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTGGCGAGt       >  1:543921/1‑140 (MQ=255)
                                                                                                                            ccGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTGGCGAGTGCATc  <  1:810196/140‑1 (MQ=255)
                                                                                                                            ccGGATTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCCCCGCAGTCAGCGCAATGTTGCTGGCGAGTGCATc  <  2:590820/140‑1 (MQ=255)
                                                                                                                                |                                                                                                                                       
CATGGTAAGCATGAGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTGGCGAGTGCATC  >  NC_000913/1186978‑1187241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑