Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,613,264	G→T	intergenic (‑13/+51)	glsB ← / ← sad	glutaminase 2/NAD(+)‑dependent succinate semialdehyde dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,613,264	0	G	T	100.0%	115.9 / NA	34	intergenic (‑13/+51)	glsB/sad	glutaminase 2/NAD(+)‑dependent succinate semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (17/17);  total (17/17)

CCCAATCGGGAACCGTCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGACCAAAGC  >  NC_000913/1613127‑1613391                                                                                                                                          |                                                                                                                                cccAATCGGGAACCGTCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGTAc                                                                                                                               >  1:430161/1‑140 (MQ=255)   cAATCGGGAACCGTCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGTACCg                                                                                                                             <  1:815183/140‑1 (MQ=255)                  tACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGTACCGCAGGTCTGAAAAGAc                                                                                                              >  1:79132/1‑140 (MQ=255)                    cTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGTACCGCAGGTCTGAAAAGACCt                                                                                                            <  1:66878/140‑1 (MQ=255)                       tAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGTACCGCAGGTCTGAAAAGACCTGCg                                                                                                         >  2:55200/1‑140 (MQ=255)                            aGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGTACCGCAGGTCTGAAAAGACCTGCGAGtat                                                                                                    >  1:166314/1‑140 (MQ=255)                            aGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGTACCGCAGGTCTGAAAAGACCTGCGAGtat                                                                                                    >  2:279718/1‑140 (MQ=255)                               gcCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGTACCGCAGGTCTGAAAAGACCTGCGAGTATATc                                                                                                 >  2:413414/1‑140 (MQ=255)                                ccGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGTACCGCAGGTCTGAAAAGACCTGCGAGTATATCa                                                                                                <  1:413414/140‑1 (MQ=255)                                 cGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGTACCGCAGGTCTGAAAAGACCTGCGAGTATATCag                                                                                               >  2:299000/1‑140 (MQ=255)                                  ggAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGTACCGCAGGTCTGAAAAGACCTGCGAGTATATCaga                                                                                              <  1:564645/140‑1 (MQ=255)                                   gAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGTACCGCAGGTCTGAAAAGACCTGCGAGTATATCagag                                                                                             <  1:157054/140‑1 (MQ=255)                                   gAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGTACCGCAGGTCTGAAAAGACCTGCGAGTATATCagag                                                                                             <  2:430161/140‑1 (MQ=255)                                             cgcgACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGTACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGt                                                                                   <  2:166314/140‑1 (MQ=255)                                              gcgACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGTACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTc                                                                                  >  2:660605/1‑140 (MQ=255)                                                         cTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGTACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATcc                                                                       >  1:59176/1‑140 (MQ=255)                                                            aCCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGTACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCgg                                                                     <  2:221408/139‑1 (MQ=255)                                                            aCCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGTACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCgg                                                                     >  1:221408/1‑139 (MQ=255)                                                            aCCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGTACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGt                                                                    >  1:876150/1‑140 (MQ=255)                                                            aCCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGTACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGt                                                                    >  1:214012/1‑140 (MQ=255)                                                                      ggCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGTACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCgg                                                                     <  1:610655/129‑1 (MQ=255)                                                                      ggCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGTACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCgg                                                                     >  2:610655/1‑129 (MQ=255)                                                                        ccGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGTACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATc                                                                        >  1:626721/1‑124 (MQ=255)                                                                        ccGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGTACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATc                                                                        <  2:626721/124‑1 (MQ=255)                                                                           cACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGTACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCt                                                     <  2:876150/140‑1 (MQ=255)                                                                           cACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGTACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCt                                                     <  2:214012/140‑1 (MQ=255)                                                                           cACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGTACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCt                                                     <  1:710099/140‑1 (MQ=255)                                                                                        aTGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGTACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGaa                                        <  1:55200/140‑1 (MQ=255)                                                                                            tttCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGTACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCg                                    >  2:533821/1‑140 (MQ=255)                                                                                                      tGCATTATCCATGGCGACTGCCACTTTCTACTCCTGTACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGa                          <  1:323426/140‑1 (MQ=255)                                                                                                      tGCATTATCCATGGCGACTGCCACTTTCTACTCCTGTACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGa                          <  1:583914/140‑1 (MQ=255)                                                                                                                  ggCGACTGCCACTTTCTACTCCTGTACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCt              >  1:131796/1‑140 (MQ=255)                                                                                                                       cTGCCACTTTCTACTCCTGTACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGa         >  1:425205/1‑140 (MQ=255)                                                                                                                              tttCTACTCCTGTACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGACCAAAGc  <  2:79132/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                CCCAATCGGGAACCGTCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGACCAAAGC  >  NC_000913/1613127‑1613391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑