Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,792,142	T→G	intergenic (‑122/+125)	ydiV ← / ← nlpC	anti‑FlhDC factor/NlpC/P60 family lipoprotein NlpC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,792,142	0	T	G	100.0%	112.1 / NA	31	intergenic (‑122/+125)	ydiV/nlpC	anti‑FlhDC factor/NlpC/P60 family lipoprotein NlpC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (12/19);  total (12/19)

TCATGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCATAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAG  >  NC_000913/1792017‑1792270                                                                                                                              |                                                                                                                                 tCATGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCATAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGGCCTTTTTTTAGAtt                                                                                                                    <  2:855149/140‑1 (MQ=255)    tGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCATAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCTGGGGGCCTTTTTTTAGATTATc                                                                                                                 <  2:704499/140‑1 (MQ=255)    tGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCATAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGGCCTTTTTTTAGATTATc                                                                                                                 <  2:547658/140‑1 (MQ=255)          ccGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCATAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGGCCTTTTTTTAGATTATCCTGAtt                                                                                                           >  2:172541/1‑140 (MQ=255)              cccTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCATAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGGCCTTTTTTTAGATTATCCTGATTATaa                                                                                                       <  1:264526/140‑1 (MQ=255)               ccTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCATAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGGCCTTTTTTTAGATTATCCTGATTATaaa                                                                                                      >  1:398586/1‑140 (MQ=255)                      aaGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCATAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGGCCTTTTTTTAGATTATCCTGATTATAAACGaataa                                                                                               <  1:298373/140‑1 (MQ=255)                          aTGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCATAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGGCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCa                                                                                           >  1:361689/1‑140 (MQ=255)                             tttttCAGTATCCGGCTAAAAGGGATGAGGCCATAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGGCCTTTTTTTAGATTATCCTGATTATAAACGa                                                                                                   <  1:503734/129‑1 (MQ=255)                             tttttCAGTATCCGGCTAAAAGGGATGAGGCCATAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGGCCTTTTTTTAGATTATCCTGATTATAAACGa                                                                                                   >  2:503734/1‑129 (MQ=255)                             tttttCAGTATCCGGCTAAAAGGGATGAGGCCATAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGGCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCAttt                                                                                        >  1:920343/1‑140 (MQ=255)                                ttCAGTATCCGGCTAAAAGGGATGAGGCCATAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGGCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACg                                                                                     <  1:144338/140‑1 (MQ=255)                                          ggCTAAAAGGGATGAGGCCATAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGGCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGt                                                                           <  2:524034/140‑1 (MQ=255)                                             tAAAAGGGATGAGGCCATAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGGCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTg                                                                        <  2:838276/140‑1 (MQ=255)                                             tAAAAGGGATGAGGCCATAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGGCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTg                                                                        <  2:361689/140‑1 (MQ=255)                                               aaaGGGATGAGGCCATAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGGCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCa                                                                      <  1:172541/140‑1 (MQ=255)                                               aaaGGGATGAGGCCATAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGGCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCa                                                                      <  1:489074/140‑1 (MQ=255)                                                  gggATGAGGCCATAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGGCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCaaaa                                                                   <  2:920343/140‑1 (MQ=255)                                                          gCCATAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGGCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAAta                                                                 >  1:572804/1‑134 (MQ=255)                                                          gCCATAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGGCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAAta                                                                 <  2:572804/134‑1 (MQ=255)                                                                aGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGGCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCa                                                     <  2:906431/140‑1 (MQ=255)                                                                     tAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGGCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGt                                                >  1:183663/1‑140 (MQ=255)                                                                      aaCAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGGCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTa                                               >  1:481842/1‑140 (MQ=255)                                                                            tACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGGCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGa                                         >  1:677843/1‑140 (MQ=255)                                                                               aGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGGCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACgg                                      <  2:183663/140‑1 (MQ=255)                                                                                   ggAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGGCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGAt                                  >  2:124192/1‑140 (MQ=255)                                                                                        aaCTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGGCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTatc                             <  2:792976/140‑1 (MQ=255)                                                                                                     tCCATGTTAACCACTTTTTCAGGGGGCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCAtt                >  1:223176/1‑140 (MQ=255)                                                                                                          gTTAACCACTTTTTCAGGGGGCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCg           <  2:677843/140‑1 (MQ=255)                                                                                                           ttAACCACTTTTTCAGGGGGCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGa          <  2:511632/140‑1 (MQ=255)                                                                                                                   tttttCAGGGGGCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAg  >  2:245416/1‑140 (MQ=255)                                                                                                                              |                                                                                                                                 TCATGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCATAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAG  >  NC_000913/1792017‑1792270

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑