Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,820,249	G→T	R322R (CGC→CGA)	chbC ←	N,N'‑diacetylchitobiose‑specific PTS enzyme IIC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,820,249	0	G	T	100.0%	207.4 / NA	61	R322R (CGC→CGA)	chbC	N,N'‑diacetylchitobiose‑specific PTS enzyme IIC component
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (27/34);  total (27/34)

TCGGTTGTACCAGTACAAACGGGATAAACATCACCGGGTTCATGATAATTGGCAGACCAAACAGAATCGGTTCGTTAATCTGGAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGGCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACTGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGCCCAGATATGGAAGGTCTTACCGGCTGCCAGCGCCGCTTCGACGGAA  >  NC_000913/1820116‑1820381                                                                                                                                      |                                                                                                                                     tCGGTTGTACCAGTACAAACGGGATAAACATCACCGGGTTCATGATAATTGGCAGACCAAACAGAATCGGTTCGTTAATCTGGAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGTCGagag                                                                                                                                <  2:3029/140‑1 (MQ=255)     ttGTACCAGTACAAACGGGATAAACATCACCGGGTTCATGATAATTGGCAGACCAAACAGAATCGGTTCGTTAATCTGGAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGTCGAGAGGCGa                                                                                                                            <  2:413062/140‑1 (MQ=255)        tACCAGTACAAACGGGATAAACATCACCGGGTTCATGATAATTGGCAGACCAAACAGAATCGGTTCGTTAATCTGGAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGTCGagag                                                                                                                                >  2:710183/1‑133 (MQ=255)        tACCAGTACAAACGGGATAAACATCACCGGGTTCATGATAATTGGCAGACCAAACAGAATCGGTTCGTTAATCTGGAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGTCGagag                                                                                                                                <  1:710183/133‑1 (MQ=255)        tACCAGTACAAACGGGATAAACATCACCGGGTTCATGATAATTGGCAGACCAAACAGAATCGGTTCGTTAATCTGGAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGTCGAGAGGCGATaa                                                                                                                         >  2:570267/1‑140 (MQ=255)         aCCAGTACAAACGGGATAAACATCACCGGGTTCATGATAATTGGCAGACCAAACAGAATCGGTTCGTTAATCTGGAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGTCGAGAGGCGATaaa                                                                                                                        >  1:437310/1‑140 (MQ=255)          ccAGTACAAACGGGATAAACATCACCGGGTTCATGATAATTGGCAGACCAAACAGAATCGGTTCGTTAATCTGGAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGTCGAGAGGCGATAAAg                                                                                                                       <  1:837956/140‑1 (MQ=255)           cAGTACAAACGGGATAAACATCACCGGGTTCATGATAATTGGCAGACCAAACAGAATCGGTTCGTTAATCTGGAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGTCGAGAGGCGATAAAGa                                                                                                                      <  1:524606/140‑1 (MQ=255)             gTACAAACGGGATAAACATCACCGGGTTCATGATAATTGGCAGACCAAACAGAATCGGTTCGTTAATCTGGATGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGTCGAGAGGCGATAAAGATa                                                                                                                    >  1:576666/1‑140 (MQ=255)              tACAAACGGGATAAACATCACCGGGTTCATGATAATTGGCAGACCAAACAGAATCGGTTCGTTAATCTGGAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGTCGAGAGGCGATAAAGATAg                                                                                                                   <  1:175773/140‑1 (MQ=255)                  aaCGGGATAAACATCACCGGGTTCATGATAATTGGCAGACCAAACAGAATCGGTTCGTTAATCTGGAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGTCGAGAGGCGATAAAGATAGCCAg                                                                                                               >  1:213349/1‑140 (MQ=255)                  aaCGGGATAAACATCACCGGGTTCATGATAATTGGCAGACCAAACAGAATCGGTTCGTTAATCTGGAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGTCGAGAGGCGATAAAGATAGCCAg                                                                                                               >  1:457757/1‑140 (MQ=255)                   aCGGGATAAACATCACCGGGTTCATGATAATTGGCAGACCAAACAGAATCGGTTCGTTAATCTGGAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGTCGAGAGGCGATAAAGATAGCCAgg                                                                                                              >  1:810320/1‑140 (MQ=255)                   aCGGGATAAACATCACCGGGTTCATGATAATTGGCAGACCAAACAGAATCGGTTCGTTAATCTGGAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTAACGATAATCAGCACGTCGAGAGGCGATAAAGATAGCCAgg                                                                                                              >  2:74810/1‑140 (MQ=255)                     gggATAAACATCACCGGGTTCATGATAATTGGCAGACCAAACAGAATCGGTTCGTTAATCTGGAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGTCGAGAGGCGATAAAGATAGCCAGGAt                                                                                                            >  1:11770/1‑140 (MQ=255)                      ggATAAACATCACCGGGTTCATGATAATTGGCAGACCAAACAGAATCGGTTCGTTAATCTGGAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGTCGAGAGGCGATAAAGATAGCCAGGATc                                                                                                           <  2:476630/140‑1 (MQ=255)                        aTAAACATCACCGGGTTCATGATAATTGGCAGACCAAACAGAATCGGTTCGTTAATCTGGAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGTCGAGAGGCGATAAAGATAGCCAGGATCAg                                                                                                         >  2:621767/1‑140 (MQ=255)                           aaCATCACCGGGTTCATGATAATTGGCAGACCAAACAGAATCGGTTCGTTAATCTGGAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTAACGATAATCAGCACGTCGAGAGGCGATAAAGATAGCCAGGATCAGGcc                                                                                                      <  1:74810/140‑1 (MQ=255)                                cACCGGGTTCATGATAATTGGCAGACCAAACAGAATCGGTTCGTTAATCTGGAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGTCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAg                                                                                                 >  1:454316/1‑140 (MQ=255)                                 aCCGGGTTCATGATAATTGGCAGACCAAACAGAATCGGTTCGTTAATCTGGAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGTCGAGAGGCGATAAAGATAGCCAGGATc                                                                                                           >  2:340794/1‑129 (MQ=255)                                 aCCGGGTTCATGATAATTGGCAGACCAAACAGAATCGGTTCGTTAATCTGGAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGTCGAGAGGCGATAAAGATAGCCAGGATc                                                                                                           <  1:340794/129‑1 (MQ=255)                                   cGGGTTCATGATAATTGGCAGACCAAACAGAATCGGTTCGTTAATCTGGAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGTCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCg                                                                                              <  2:810320/140‑1 (MQ=255)                                     ggTTCATGATAATTGGCAGACCAAACAGAATCGGTTCGTTAATCTGGATGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGTCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGca                                                                                            <  2:576666/140‑1 (MQ=255)                                     ggTTCATGATAATTGGCAGACCAAACAGAATCGGTTCGTTAATCTGGAAGATGCCGGTCGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGTCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGca                                                                                            >  1:196020/1‑140 (MQ=255)                                     ggTTCATGATAATTGGCAGACCAAACAGAATCGGTTCGTTAATCTGGAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGTCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGca                                                                                            >  1:97278/1‑140 (MQ=255)                                       ttCATGATAATTGGCAGACCAAACAGAATCGGTTCGTTAATCTGGAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGTCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGcacc                                                                                          >  2:41729/1‑140 (MQ=255)                                          aTGATAATTGGCAGACCAAACAGAATCGGTTCGTTAATCTGGAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGTCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACt                                                                                       <  2:457757/140‑1 (MQ=255)                                           tGATAATTGGCAGACCAAACAGAATCGGTTCGTTAATCTGGAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGTCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACTg                                                                                      <  2:354732/140‑1 (MQ=255)                                                aTTGGCAGACCAAACAGAATCGGTTCGTTAATCTGGAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGTCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCGCCACTGccccc                                                                                 <  2:243373/140‑1 (MQ=255)                                                aTTGGCAGACCAAACAGAATCGGTTCGTTAATCTGGAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGTCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACTGccccc                                                                                 <  2:454316/140‑1 (MQ=255)                                                aTTGGCAGACCAAACAGAATCGGTTCGTTAATCTGGAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGTCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACTGccccc                                                                                 <  2:349974/140‑1 (MQ=255)                                                   ggCAGACCAAACAGAATCGGTTCGTTAATCTGGAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGTCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACTGCCCCCAAg                                                                              <  2:56301/140‑1 (MQ=255)                                                   ggCAGACCAAACAGAATCGGTTCGTTAATCTGGAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGTCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACTGCCCCCAAg                                                                              <  2:97278/140‑1 (MQ=255)                                                   ggCAGACCAAACAGAATCGGTTCGTTAATCTGGAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGTCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACTGCCCCCAAg                                                                              <  2:213349/140‑1 (MQ=255)                                                   ggCAGACCAAACAGAATCGGTTCGTTAATCTGGAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGTCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACTGCCCCCAAg                                                                              <  1:621767/140‑1 (MQ=255)                                                      aGACCAAACAGAATCGGTTCGTTAATCCGGAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGTCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACTGCCCCCAAGGaa                                                                           >  2:806556/1‑140 (MQ=255)                                                       gACCAAACAGAATCGGTTCGTTAATCTGGAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGTCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACTGCCCCCAAGGaaa                                                                          >  2:36371/1‑140 (MQ=255)                                                             aCAGAATCGGTTCGTTAATCTGGAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGTCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACTGCCCCCAAGGAAAATAAAg                                                                    <  2:437310/140‑1 (MQ=255)                                                             aCAGAATCGGTTCGTTAATCTGGAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGTCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACTGCCCCCAAGGAAAATAAAg                                                                    <  2:707644/140‑1 (MQ=255)                                                              cAGAATCGGTTCGTTAATCTGGAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGTCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACTGCCCCCAAGGAAAATAAAgg                                                                   >  1:10565/1‑140 (MQ=255)                                                                 aaTCGGTTCGTTAATCTGGAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGTCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACTGCCCCCAAGGAAAATAAAGGAGt                                                                <  1:372542/140‑1 (MQ=255)                                                                  aTCGGTTCGTTAATCTGGAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGTCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACTGCCCCCAAGGAAAATAAAGGAGTc                                                               <  1:41729/140‑1 (MQ=255)                                                                      gTTCGTTAATCTGGAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGTCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACTGCCCCCAAGGAAAATAAAGGAGTCCAGc                                                           >  2:467433/1‑140 (MQ=255)                                                                        tCGTTAATCTGGAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGTCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACTGCCCCCAAGGAAAATAAAGGAGTCCAGCAt                                                         <  1:806556/140‑1 (MQ=255)                                                                            tAATCTGGAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGTCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACTGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGc                                                     <  2:11770/140‑1 (MQ=255)                                                                             aaTCTGGAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGTCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACTGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCt                                                    <  2:10565/140‑1 (MQ=255)                                                                             aaTCTGGAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGTCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACTGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCt                                                    <  1:234861/140‑1 (MQ=255)                                                                             aaTCTGGAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGTCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACTGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCt                                                    <  1:570267/140‑1 (MQ=255)                                                                                      gATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGTCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACTGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGCCCAGa                                           >  1:719279/1‑140 (MQ=255)                                                                                        tGCCGGACGGCAGCGCCAGTTTTGTCACCTGACGATAATCAGCACGTCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACTGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGCCCAGata                                         >  1:114020/1‑140 (MQ=255)                                                                                        tGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGTCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACTGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGCCCAGata                                         >  1:549424/1‑140 (MQ=255)                                                                                        tGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGTCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACTGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGCCCAGata                                         >  1:635833/1‑140 (MQ=255)                                                                                        tGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGTCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACTGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGCCCAGata                                         >  2:879253/1‑140 (MQ=255)                                                                                         gCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGTCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACTGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGCCCAGatat                                        >  2:748792/1‑140 (MQ=255)                                                                                                                   ccTGACGGTAATCAGCACGTCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACTGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGCCCAGATATGGAAGGTCTTACCGGc                        >  2:759746/1‑130 (MQ=255)                                                                                                                   ccTGACGATAATCAGCACGTCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACTGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGCCCAGATATGGAAGGTCTTACCGGc                        <  1:759746/130‑1 (MQ=255)                                                                                                                     tGACGATAATCAGCACGTCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACTGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGCCCAGATATGGAAGGTCTTACCGGCTGCCAGcgccgc            >  2:694386/1‑140 (MQ=255)                                                                                                                     tGACGATAATCAGCACGTCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACTGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGCCCAGATATGGAAGGTCTTACCGGCTGCCAGcgccgc            <  1:36371/140‑1 (MQ=255)                                                                                                                             aTCAGCACGTCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACTGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGCCCAGATATGGAAGGTCTTACCGGCTGCCAGCGCCGCTTCGACgg    <  2:719279/140‑1 (MQ=255)                                                                                                                               cAGCACGTCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACTGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGCCCAGATATGGAAGGTCTTACCGGCTGCCAGCGCCGCTTCGACGGaa  <  2:635833/140‑1 (MQ=255)                                                                                                                               cAGCACGTCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACTGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGCCCAGATATGGAAGGTCTTACCGGCTGCCAGCGCCGCTTCGACGGaa  <  2:549424/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                     TCGGTTGTACCAGTACAAACGGGATAAACATCACCGGGTTCATGATAATTGGCAGACCAAACAGAATCGGTTCGTTAATCTGGAAGATGCCGGACGGCAGCGCCAGTTTTGCCACCTGACGATAATCAGCACGGCGAGAGGCGATAAAGATAGCCAGGATCAGGCCTAAAGTCGCACCACTGCCCCCAAGGAAAATAAAGGAGTCCAGCATCGGCTTGGCCCAGATATGGAAGGTCTTACCGGCTGCCAGCGCCGCTTCGACGGAA  >  NC_000913/1820116‑1820381

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑