Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,615,053	Δ1 bp	coding (1387/1575 nt)	nikA →	Ni(2(+)) ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,615,053	0	A	.	100.0%	321.0 / NA	75	coding (1387/1575 nt)	nikA	Ni(2(+)) ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (34/41);  total (34/41)

CCTGGGGCGCGCCATATGATCCACACGCCTTCCTCAGTTCAATGCGCGTACCGTCACACGCTGACTTCCAGGCACAGCAAGGATTAGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCGACCCATGACGAAACGCAACGTCAGGCGCTGTATCGCGACATTCTGACCCGTCTGCATGACGAGGCGGTTTATCTGCCTATCAGTTACATCTCAATGATGGTGGTATCAAAACCGGAGCTGGGTAACATCCCCT  >  NC_000913/3614922‑3615185                                                                                                                                    |                                                                                                                                     ccTGGGGCGCGCCATATGATCCACACGCCTTCCTCAGTTCAATGCGCGTACCGTCACACGCTGACTTCCAGGCACAGCAAGGATTAGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACg                                                                                                                             <  1:352575/140‑1 (MQ=255)          cgcCATATGATCCACACGCCTTCCTCAGTTCAATGCGCGTACCGTCACACGCTGACTTCCAGGCACAGCAAGGATTAGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAAc                                                                                                                    >  2:346229/1‑140 (MQ=255)          cgcCATATGATCCACACGCCTTCCTCAGTTCAATGCGCGTACCGTCACACGCTGACTTCCAGGCACAGCAAGGATTAGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAAc                                                                                                                    >  1:781458/1‑140 (MQ=255)          cgcCATATGATCCACACGCCTTCCTCAGTTCAATGCGCGTACCGTCACACGCTGACTTCCAGGCACAGCAAGGATTAGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAAc                                                                                                                    >  2:1034703/1‑140 (MQ=255)          cgcCATATGATCCACACGCCTTCCTCAGTTCAATGCGCGTACCGTCACACGCTGACTTCCAGGCACAGCAAGGATTAGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAAc                                                                                                                    >  1:146537/1‑140 (MQ=255)             cATATGATCCACACGCCTTCCTCAGTTCAATGCGCGTACCGTCACACGCTGACTTCCAGGCACAGCAAGGATTAGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTc                                                                                                                 <  2:75067/140‑1 (MQ=255)             cATATGATCCACACGCCTTCCTCAGTTCAATGCGCGTACCGTCACACGCTGACTTCCAGGCACAGCAAGGATTAGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTc                                                                                                                 <  2:566251/140‑1 (MQ=255)               tatGATCCACACGCCTTCCTCAGTTCAATGCGCGTACCGTCACACGCTGACTTCCAGGCACAGCAAGGATTAGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAg                                                                                                               <  2:1030163/140‑1 (MQ=255)                atGATCCACACGCCTTCCTCAGTTCAATGCGCGTACCGTCACACGCTGACTTCCAGGCACAGCAAGGATTAGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAgg                                                                                                              <  1:1071253/140‑1 (MQ=255)                  gATCCACACGCCTTCCTCAGTTCAATGCGCGTACCGTCACACGCTGACTTCCAGGCACAGCAAGGATTAGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGgcg                                                                                                            <  2:85318/140‑1 (MQ=255)                  gATCCACACGCCTTCCTCAGTTCAATGCGCGTACCGTCACACGCTGACTTCCAGGCACAGCAAGGATTAGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGgcg                                                                                                            <  2:702910/140‑1 (MQ=255)                  gATCCACACGCCTTCCTCAGTTCAATGCGCGTACCGTCACACGCTGACTTCCAGGCACAGCAAGGATTAGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGgcg                                                                                                            <  1:272212/140‑1 (MQ=255)                     ccACACGCCTTCCTCAGTTCAATGCGCGTACCGTCACACGCTGACTTCCAGGCACAGCAAGGATTAGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGGCGCTg                                                                                                         >  2:82055/1‑140 (MQ=255)                       acacGCCTTCCTCAGTTCAATGCGCGTACCGTCACACGCTGACTTCCAGGCACAGCAAGGATTAGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGgcgc                                                                                                           <  2:1026589/136‑1 (MQ=255)                       acacGCCTTCCTCAGTTCAATGCGCGTACCGTCACACGCTGACTTCCAGGCACAGCAAGGATTAGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGgcgc                                                                                                           >  1:1026589/1‑136 (MQ=255)                       acacGCCTTCCTCAGTTCAATGCGCGTACCGTCACACGCTGACTTCCAGGCACAGCAAGGATTAGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGGCGCTGTa                                                                                                       >  2:337699/1‑140 (MQ=255)                       acacGCCTTCCTCAGTTCAATGCGCGTACCGTCACACGCTGACTTCCAGGCACAGCAAGGATTAGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGGCGCTGTa                                                                                                       >  2:268341/1‑140 (MQ=255)                       acacGCCTTCCTCAGTTCAATGCGCGTACCGTCACACGCTGACTTCCAGGCACAGCAAGGATTAGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGGCGCTGTa                                                                                                       >  1:131828/1‑140 (MQ=255)                         acGCCTTCCTCAGTTCAATGCGCGTACCGTCACACGCTGACTTCCAGGCACAGCAAGGATTAGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGgcg                                                                                                            <  2:260429/133‑1 (MQ=255)                         acGCCTTCCTCAGTTCAATGCGCGTACCGTCACACGCTGACTTCCAGGCACAGCAAGGATTAGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGgcg                                                                                                            >  1:260429/1‑133 (MQ=255)                         acGCCTTCCTCAGTTCAATGCGCGTACCGTCACACGCTGACTTCCAGGCACAGCAAGGATTAGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGGCGCTGTATc                                                                                                     <  2:990039/140‑1 (MQ=255)                         acGCCTTCCTCAGTTCAATGCGCGTACCGTCACACGCTGACTTCCAGGCACAGCAAGGATTAGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGGCGCTGTATc                                                                                                     >  1:990039/1‑140 (MQ=255)                         acGCCTTCCTCAGTTCAATGCGCGTACCGTCACACGCTGACTTCCAGGCACAGCAAGGATTAGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGGCGCTGTATc                                                                                                     >  1:399338/1‑140 (MQ=255)                            ccTTCCTCAGTTCAATGCGCGTACCGTCACACGCTGACTTCCAGGCACAGCAAGGATTAGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGGCGCTGTATcgcg                                                                                                  <  1:637789/140‑1 (MQ=255)                            ccTTCCTCAGTTCAATGCGCGTACCGTCACACGCTGACTTCCAGGCACAGCAAGGATTAGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGGCGCTGTATcgcg                                                                                                  <  2:781458/140‑1 (MQ=255)                            ccTTCCTCAGTTCAATGCGCGTACCGTCACACGCTGACTTCCAGGCACAGCAAGGATTAGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGGCGCTGTATcgcg                                                                                                  <  1:337699/140‑1 (MQ=255)                             cTTCCTCAGTTCAATGCGCGTACCGTCACACGCTGACTTCCAGGCACAGCAAGGATTAGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGGCGCTGTATCGCGa                                                                                                 <  1:1034703/140‑1 (MQ=255)                              ttCCTCAGTTCAATGCGCGTACCGTCACACGCTGACTTCCAGGCACAGCAAGGATTAGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGGCGCTGTATCGCGAc                                                                                                >  2:678436/1‑140 (MQ=255)                               tCCTCAGTTCAATGCGCGTACCGTCACACGCTGACTTCCAGGCACAGCAAGGATTAGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGGCGCTGTATCGCGACa                                                                                               >  1:198490/1‑140 (MQ=255)                               tCCTCAGTTCAATGCGCGTACCGTCACACGCTGACTTCCAGGCACAGCAAGGATTAGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGGCGCTGTATCGCGACa                                                                                               >  2:604069/1‑140 (MQ=255)                                ccTCAGTTCAATGCGCGTACCGTCACACGCTGACTTCCAGGCACAGCAAGGATTAGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGGCGCTGTATCGCGACAt                                                                                              >  2:710273/1‑140 (MQ=255)                                 cTCAGTTCAATGCGCGTACCGTCACACGCTGACTTCCAGGCACAGCAAGGATTAGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGGCGCTGTATCGCGACAtt                                                                                             >  1:295396/1‑140 (MQ=255)                                   cAGTTCAATGCGCGTACCGTCACACGCTGACTTCCAGGCACAGCAAGGATTAGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGGCGCTGTATCGCGACATTCt                                                                                           <  2:399338/140‑1 (MQ=255)                                     gTTCAATGCGCGTACCGTCACACGCTGACTTCCAGGCACAGCAAGGATTAGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGGCGCTGTATCGCGACATTCTGa                                                                                         <  1:865551/140‑1 (MQ=255)                                        cAATGCGCGTACCGTCACACGCTGACTTCCAGGCACAGCAAGGATTAGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGGCGCTGTATCGCGACATTCTGAccc                                                                                      <  1:604069/140‑1 (MQ=255)                                         aaTGCGCGTACCGTCACACGCTGACTTCCAGGCACAGCAAGGATTAGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGGCGCTGTATCGCGACATTCTGACCCg                                                                                     <  2:131828/140‑1 (MQ=255)                                           tGCGCGTACCGTCACACGCTGACTTCCAGGCACAGCAAGGATTAGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGGCGCTGTATCGCGACATTCTGa                                                                                         >  2:271738/1‑134 (MQ=255)                                           tGCGCGTACCGTCACACGCTGACTTCCAGGCACAGCAAGGATTAGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGGCGCTGTATCGCGACATTCTGa                                                                                         <  1:271738/134‑1 (MQ=255)                                           tGCGCGTACCGTCACACGCTGACTTCCAGGCACAGCAAGGATTAGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGGCGCTGTATCGCGACATTCTGACCCGTc                                                                                   >  2:564724/1‑140 (MQ=255)                                                    cGTCACACGCTGACTTCCAGGCACAGCAAGGATTAGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGGCGCTGTATCGCGACATTCTGACCCGTCTGCATGACg                                                                          <  1:113474/140‑1 (MQ=255)                                                     gTCACACGCTGACTTCCAGGCACAGCAAGGATTAGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGGCGCTGTATCGCGACATTCTGACCCGTCTGCATGACGa                                                                         <  2:295396/140‑1 (MQ=255)                                                       cacaCGCTGACTTCCAGGCACAGCAAGGATTAGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGGCGCTGTATCGCGACATTCTGACCCGTCTGCATGACGAgg                                                                       >  2:276579/1‑140 (MQ=255)                                                         cacGCTGACTTCCAGGCACAGCAAGGATTAGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGGCGCTGTATCGCGACATTCTGACCCGTCTGCATGACGAGGCg                                                                     <  1:276579/140‑1 (MQ=255)                                                            gCTGACTTCCAGGCACAGCAAGGATTAGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGGCGCTGTATCGCGACATTCTGACCCGTCTGCATGACGAGGCGGtt                                                                  >  2:539382/1‑140 (MQ=255)                                                                aCTTCCAGGCACAGCAAGGATTAGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGGCGCTGTATCGCGACATTCTGACCCGTCTGCATGACGAGGCGGTTTATc                                                              >  2:1028407/1‑140 (MQ=255)                                                                aCTTCCAGGCACAGCAAGGATTAGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGGCGCTGTATCGCGACATTCTGACCCGTCTGCATGACGAGGCGGTTTATc                                                              >  2:411959/1‑140 (MQ=255)                                                                 cTTCCAGGCACAGCAAGGATTAGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑TCCATGACGAAACGCAACGTCAGGCGCTGTATCGCGACATTCTGACCCGTCTGCATGACGAGGCGGTTTATCt                                                             <  1:848710/140‑1 (MQ=255)                                                                   tCCAGGCACAGCAAGGATTAGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGGCGCTGTATCGTGACATTCTGACCCGTCTGCATGACGAGGCGGTTTATCTGc                                                           <  1:82055/140‑1 (MQ=255)                                                                   tCCAGGCACAGCAAGGATTAGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGGCGCTGTATCGCGACATTCTGACCCGTCTGCATGACGAGGCGGTTTATCTGc                                                           <  1:564724/140‑1 (MQ=255)                                                                   tCCAGGCACAGCAAGGATTAGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGGCGCTGTATCGCGACATTCTGACCCGTCTGCATGACGAGGCGGTTTATCTGc                                                           <  2:146537/140‑1 (MQ=255)                                                                     cAGGCACAGCAAGGATTAGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGGCGCTGTATCGCGACATTCTGACCCGTCTGCATGACGAGGCGGTTTATCTGCCt                                                         <  2:302640/140‑1 (MQ=255)                                                                      aGGCACAGCAAGGATTAGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGGCGCTGTATCGCGACATTCTGACCCGTCTGCATGACGAGGCGGTTTATCTGCCTa                                                        <  1:1028407/140‑1 (MQ=255)                                                                       ggCACAGCAAGGATTAGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGGCGCTGTATCGCGACATTCTGACCCGTCTGCATGACGAGGCGGTTTATCTGCCTAt                                                       <  1:539382/140‑1 (MQ=255)                                                                           caGCAAGGATTAGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGGCGCTGTATCGCGACATTCTGACCCGTCTGCATGACGAGGCGGTTTATCTGCCTATCAGt                                                   <  1:268341/140‑1 (MQ=255)                                                                           caGCAAGGATTAGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGGCGCTGTATCGCGACATTCTGACCCGTCTGCATGACGAGGCGGTTTATCTGCCTATCAGt                                                   <  1:678436/140‑1 (MQ=255)                                                                            aGCAAGGATTAGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGGCGCTGTATCGCGACATTCTGACCCGTCTGCATGACGAGGCGGTTTATCTGCCTATCAGtt                                                  >  2:241252/1‑140 (MQ=255)                                                                            aGCAAGGATTAGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGGCGCTGTATCGCGACATTCTGACCCGTCTGCATGACGAGGCGGTTTATCTGCCTATCAGtt                                                  <  1:346229/140‑1 (MQ=255)                                                                            aGCAAGGATTAGCCGACAAACCGCTGATTGACAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGGCGCTGTATCGCGACATTCTGACCCGTCTGCATGACGAGGCGGTTTATCTGCCTATCAGtt                                                  >  1:567699/1‑140 (MQ=255)                                                                             gCAAGGATTAGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGGCGCTGTATCGCGACATTCTGACCCGTCTGCATGACGAGGCGGTTTATCTGCCTATCAGTTa                                                 <  1:241252/140‑1 (MQ=255)                                                                                   aTTAGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGGCGCTGTATCGCGACATTCTGACCCGTCTGCATGACGAGGCGGTTTATCTGCCTATCAGTTACAtctc                                           >  2:651488/1‑140 (MQ=255)                                                                                      aGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGGCGCTGTATCGCGACATTCTGACCCGTCTGCATGACGGGGCGGTTTATCTGCCTa                                                        <  2:304108/124‑1 (MQ=255)                                                                                      aGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGGCGCTGTATCGCGACATTCTGACCCGTCTGCATGACGGGGCGGTTTATCTGCCTa                                                        >  1:304108/1‑124 (MQ=255)                                                                                      aGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGGCGCTGTATCGCGACATTCTGACCCGTCTGCATGACGAGGCGGTTTATCTGCCTATCAGt                                                   <  2:230417/129‑1 (MQ=255)                                                                                      aGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGGCGCTGTATCGCGACATTCTGACCCGTCTGCATGACGAGGCGGTTTATCTGCCTATCAGTTACATCTCAat                                        >  2:884648/1‑140 (MQ=255)                                                                                      aGCCGACAAACCGCTGATTGATAAAGACATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGGCGCTGTATCGCGACATTCTGACCCGTCTGCATGACGAGGCGGTTTATCTGCCTATCAGt                                                   >  1:230417/1‑129 (MQ=255)                                                                                        ccGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGGCGCTGTATCGCGACATTCTGACCCGTCTGCATGACGAGGCGGTTTATCTGCCTATCAGTTACATCTCAatga                                      >  2:480116/1‑140 (MQ=255)                                                                                             aaaCCGCTGATTGACAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGGCGCTGTATCGCGACATTCTGACCCGTCTGCATGACGAGGCGGTTTATCTGCCTATCAGTTACATCTCAATGAtggtg                                 <  2:567699/140‑1 (MQ=255)                                                                                                  gCTGATTGATAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGGCGCTGTATCGCGACATTCTGACCCGTCTGCATGACGAGGCGGTTTATCTGCCTATCAGTTACATCTCAATGATGGTGGTATc                            >  2:1038357/1‑140 (MQ=255)                                                                                                         gatAAAGAGATCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGGCGCTGTATCGCGACATTCTGACCCGTCTGCATGACGAGGCGGTTTATCTGCCTATCAGTTACATCTCAATGATGGTGGTATCAAAACCg                     <  2:679865/140‑1 (MQ=255)                                                                                                                agaTCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGGCGCTGTATCGCGACATTCTGACCCGTCTGCATGACGAGGCGGTTTATCTGCCTATCAGTTACATCTCAATGATGGTGGTATCAAAACCGGAGCTgg              <  1:710273/140‑1 (MQ=255)                                                                                                                  aTCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGGCGCTGTATCGCGACATTCTGACCCGTCTGCATGACGAGGCGGTTTATCTGCCTATCAGTTACATCTCAATGATGGTGGTATCAAAACCGGAGCTGGGt            >  2:26595/1‑140 (MQ=255)                                                                                                                  aTCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGGCGCTGTATCGCGACATTCTGACCCGTCTGCATGACGAGGCGGTTTATCTGCCTATCAGTTACATCTCAATGATGGTGGTATCAAAACCGGAGCTGGGt            <  1:804129/140‑1 (MQ=255)                                                                                                                  aTCGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGGCGCTGTATCGCGACATTCTGACCCGTCTGCATGACGAGGCGGTTTATCTGCCTATCAGTTACATCTCAATGATGGTGGTATCAAAACCGGAGCTGGGt            <  2:148113/140‑1 (MQ=255)                                                                                                                    cGGCGTAGTGCTGGCG‑CCCAAGAGGAAACGCAACGTCAGGCGCTGTATCGCGACATTCTGACCCGTCTGCATGACGAGGCGGTTTATCTTGCTATCCGTTTCATCTAAATGAAGGGGGTATCTCAACCAGAGCGGGGTaa          >  2:804129/1‑140 (MQ=255)                                                                                                                    cGGCGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGGCGCTGTATCGCGACATTCTGACCCGTCTGCATGACGAGGCGGTTTATCTGCCTATCAGTTACATCTCAATGATGGTGGTATCAAAACCGGAGCTGGGTaa          >  2:654045/1‑140 (MQ=255)                                                                                                                       cGAAGTGCTGGCG‑CCCATGACGAAACGCAACGTCAGGCGCTGTATCGCGACATTCTGACCCGTCTGCATGACGAGGCGGTTTATCTGCCTATCAGTTACATCTCAATGATGGTGGTATCAAAACCGGAGCTGGGTAACAt       <  1:654045/140‑1 (MQ=255)                                                                                                                            tGCTGGCG‑CCCATGACGAAACGCAACGTCAGGCGCTGTATCGCGACATTCTGACCCGTCTGCATGACGAGGCGGTTTATCTGCCTATCAGTTACATCTCAATGATGGTGGTATCAAAACCGGAGCTGGGTAACATCCCCt  >  2:870531/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                     CCTGGGGCGCGCCATATGATCCACACGCCTTCCTCAGTTCAATGCGCGTACCGTCACACGCTGACTTCCAGGCACAGCAAGGATTAGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCGACCCATGACGAAACGCAACGTCAGGCGCTGTATCGCGACATTCTGACCCGTCTGCATGACGAGGCGGTTTATCTGCCTATCAGTTACATCTCAATGATGGTGGTATCAAAACCGGAGCTGGGTAACATCCCCT  >  NC_000913/3614922‑3615185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑