Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,609,359	G→T	S48I (AGC→ATC)	yhhQ →	putative queuosine precursor transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,609,359	0	G	T	100.0%	318.5 / NA	90	S48I (AGC→ATC)	yhhQ	putative queuosine precursor transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (45/45);  total (45/45)

TTCTCGCAAACTCAACGCTATAAGGCGTTGTTCTGGTTATCGTTATTTCATCTGCTGGTGATCACCTCCAGTAACTATCTGGTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCT‑GGGGCGCGTTTAGCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCTCGTCGCTATTCTATATGGG  >  NC_000913/3609226‑3609494                                                                                                                                       |                                                                                                                                        ttCTCGCAAACTCAACGCTATAAGGCGTTGTTCTGGTTATCGTTATTTCATCTGCTGGTGATCACCTCCAGTAACTATCTGGTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCT‑GGGGCGCGTTTATCTTTcc                                                                                                                                   <  2:171414/140‑1 (MQ=255) ttCTCGCAAACTCAACGCTATAAGGCGTTGTTCTGGTTATCGTTATTTCATCTGCTGGTGATCACCTCCAGTAACTATCTGGTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCT‑GGGGCGCGTTTATCTTTcc                                                                                                                                   >  2:1027499/1‑140 (MQ=255) ttCTCGCAAACTCAACGCTATAAGGCGTTGTTCTGGTTATCGTTATTTCATCTGCTGGTGATCACCTCCAGTAACTATCTGGTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCT‑GGGGCGCGTTTATCTTTcc                                                                                                                                   >  2:117483/1‑140 (MQ=255)       cAAACTCAACGCTATAAGGCGTTGTTCTGGTTATCGTTATTTCATCTGCTGGTGATCACCTCCAGTAACTATCTGGTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCT‑GGGGCGCGTTTATCTTTCCGTTTAt                                                                                                                             >  1:133546/1‑140 (MQ=255)                   tataAGGCGTTGTTCTGGTTATCGTTATTTCATCTGCTGGTGATCACCTCCAGTAACTATCTGGTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTAc                                                                                                                 <  2:5588/140‑1 (MQ=255)                          cGTTGTTCTGGTTATCGTTATTTCATCTGCTGGTGATCACCTCCAGTAACTATCTGGTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTg                                                                                                          >  1:990552/1‑140 (MQ=255)                          cGTTGTTCTGGTTATCGTTATTTCATCTGCTGGTGATCACCTCCAGTAACTATCTGGTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTg                                                                                                          >  1:623712/1‑140 (MQ=255)                            ttgttCTGGTTATCGTTATTTCATCTGCTGGTGATCACCTCCAGTAACTATCTGGTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGAc                                                                                                        >  1:693639/1‑140 (MQ=255)                            ttgttCTGGTTATCGTTATTTCATCTGCTGGTGATCACCTCCAGTAACTATCTGGTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGAc                                                                                                        <  1:364904/140‑1 (MQ=255)                             tgttCTGGTTATCGTTATTTCATCTGCTGGTGATCACCTCCAGTAACTATCTGGTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGAcc                                                                                                       >  1:452011/1‑140 (MQ=255)                                         cGTTATTTCATCTGCTGGTGATCACCTCCAGTAACTATCTGGTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTAttttt                                                                                           >  2:1073771/1‑140 (MQ=255)                                              tttCATCTGCTGGTGATCACCTCCAGTAACTATCTGGTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGgcgc                                                                                      >  1:1057332/1‑140 (MQ=255)                                                  aTCTGCTGGTGATCACCTCCAGTAACTATCTGGTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCg                                                                                  >  1:301814/1‑140 (MQ=255)                                                   tCTGCTGGTGATCACCTCCAGTAACTATCTGGTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCTGGGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGgcg                                                                                       <  1:599087/135‑1 (MQ=255)                                                   tCTGCTGGTGATCACCTCCAGTAACTATCTGGTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCTGGGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGgcg                                                                                       >  2:599087/1‑135 (MQ=255)                                                   tCTGCTGGTGATCACCTCCAGTAACTATCTGGTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGc                                                                                 >  2:967457/1‑140 (MQ=255)                                                       ctgGTGATCACCTCCAGTAACTATCTGGTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGc                                                                             <  1:1027499/140‑1 (MQ=255)                                                         ggTGATCACCTCCAGTAACTATCTGGTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGccc                                                                           <  2:623712/140‑1 (MQ=255)                                                         ggTGATCACCTCCAGTAACTATCTGGTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGccc                                                                           <  1:967457/140‑1 (MQ=255)                                                         ggTGATCACCTCCAGTAACTATCTGGTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGccc                                                                           <  2:452011/140‑1 (MQ=255)                                                         ggTGATCACCTCCAGTAACTATCTGGTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGccc                                                                           <  1:904570/140‑1 (MQ=255)                                                          gTGATCACCTCCAGTAACTATCTGGTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCT‑‑GGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCg                                                                                  >  1:324922/1‑131 (MQ=255)                                                          gTGATCACCTCCAGTAACTATCTGGTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCT‑‑GGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCg                                                                                  <  2:324922/131‑1 (MQ=255)                                                           tGATCACCTCCAGTAACTATCTGGTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGa                                                                         <  2:967766/140‑1 (MQ=255)                                                             aTCACCTCCAGTAACTATCTGGTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGgcg                                                                                       <  1:683219/124‑1 (MQ=255)                                                             aTCACCTCCAGTAACTATCTGGTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGgcg                                                                                       >  2:683219/1‑124 (MQ=255)                                                             aTCACCTCCAGTAACTATCTGGTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGccc                                                                           >  2:245080/1‑136 (MQ=255)                                                             aTCACCTCCAGTAACTATCTGGTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGccc                                                                           <  1:245080/136‑1 (MQ=255)                                                               cACCTCCAGTAACTATCTGGTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCa                                                                     >  1:138318/1‑140 (MQ=255)                                                                 ccTCCAGTAACTATCTGGTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCAtt                                                                   >  1:499261/1‑140 (MQ=255)                                                                 ccTCCAGTAACTATCTGGTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCAtt                                                                   >  2:377154/1‑140 (MQ=255)                                                                 ccTCCAGTAACTATCTGGTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCAtt                                                                   >  2:485751/1‑140 (MQ=255)                                                                    ccAGTAACTATCTGGTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATc                                                                >  2:84225/1‑140 (MQ=255)                                                                    ccAGTAACTATCTGGTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATc                                                                >  1:97823/1‑140 (MQ=255)                                                                       gTAACTATCTGGTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTc                                                             >  1:919394/1‑140 (MQ=255)                                                                        tAACTATCTGGTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTcg                                                            <  1:377154/140‑1 (MQ=255)                                                                        tAACTATCTGGTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTcg                                                            <  1:469629/140‑1 (MQ=255)                                                                        tAACTATCTGGTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTcg                                                            <  1:548487/140‑1 (MQ=255)                                                                          aCTATCTGGTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTcgcg                                                          <  1:84225/140‑1 (MQ=255)                                                                          aCTATCTGGTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTcgcg                                                          <  1:485751/140‑1 (MQ=255)                                                                            tATCTGGTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGt                                                        >  1:611627/1‑140 (MQ=255)                                                                            tATCTGGTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGt                                                        >  1:240201/1‑140 (MQ=255)                                                                             aTCTGGTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTa                                                       >  1:774908/1‑140 (MQ=255)                                                                              tCTGGTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTa                                                       >  2:29965/1‑139 (MQ=255)                                                                              tCTGGTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTa                                                       <  1:29965/139‑1 (MQ=255)                                                                               cTGGTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAAt                                                     >  1:1007990/1‑140 (MQ=255)                                                                               cTGGTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAAt                                                     >  2:974602/1‑140 (MQ=255)                                                                               cTGGTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAAt                                                     >  1:43669/1‑140 (MQ=255)                                                                                 ggTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGa                                                   >  1:701068/1‑140 (MQ=255)                                                                                  gTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGAt                                                  >  1:890254/1‑140 (MQ=255)                                                                                  gTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGAt                                                  >  2:244319/1‑140 (MQ=255)                                                                                  gTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGAt                                                  >  1:565701/1‑140 (MQ=255)                                                                                  gTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGAt                                                  >  1:554334/1‑140 (MQ=255)                                                                                     cAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATccc                                               <  2:499261/140‑1 (MQ=255)                                                                                         ttCCCGTCTCCATTTTGGGTTTCCATACCACCT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCg                                           <  2:43669/140‑1 (MQ=255)                                                                                             cGTCTCCATTTTGGGTTTCCATACCACCT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGttat                                       <  2:1007990/140‑1 (MQ=255)                                                                                                     ttttGGGTTTCCATACCACCT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTcc                               >  1:645775/1‑140 (MQ=255)                                                                                                        tGGGTTTCCATACCACCT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTAc                            >  1:860837/1‑140 (MQ=255)                                                                                                          ggTTTCCATACCACCT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGt                          >  2:1008906/1‑140 (MQ=255)                                                                                                             ttCCATACCACCT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCAt                       >  1:428706/1‑140 (MQ=255)                                                                                                             ttCCATACCACCT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCAt                       >  1:694077/1‑140 (MQ=255)                                                                                                               ccATACCACCT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCAtct                     <  2:554334/140‑1 (MQ=255)                                                                                                               ccATACCACCT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCAtct                     <  2:565701/140‑1 (MQ=255)                                                                                                               ccATACCACCT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCAtct                     <  2:334412/140‑1 (MQ=255)                                                                                                               ccATACCACCT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCAtct                     <  2:428706/140‑1 (MQ=255)                                                                                                                  tACCACCT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCtcgt                  <  2:240201/140‑1 (MQ=255)                                                                                                                  tACCACCT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCtcgt                  <  1:117483/140‑1 (MQ=255)                                                                                                                    ccaccT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCGCCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCtcgtcg                <  2:133546/140‑1 (MQ=255)                                                                                                                    ccaccT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCtcgtcg                <  2:97823/140‑1 (MQ=255)                                                                                                                    ccaccT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCtcgtcg                <  1:244319/140‑1 (MQ=255)                                                                                                                      accT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCTCGTCGCt              <  2:890254/140‑1 (MQ=255)                                                                                                                       ccT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCTCGTCGCTa             <  1:1008906/140‑1 (MQ=255)                                                                                                                       ccT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCTCGTCGCTa             >  1:579474/1‑140 (MQ=255)                                                                                                                       ccT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCTCGTCGCTa             <  1:428327/140‑1 (MQ=255)                                                                                                                       ccT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCTCGTCGCTa             <  1:1056274/140‑1 (MQ=255)                                                                                                                       ccT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCTCGTCGCTa             <  2:919394/140‑1 (MQ=255)                                                                                                                       ccT‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCTCGTCGCTa             <  2:860837/140‑1 (MQ=255)                                                                                                                         t‑GGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCTCGTCGCTAtt           <  2:138318/140‑1 (MQ=255)                                                                                                                           ggggCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCtcgtcg                <  2:199507/134‑1 (MQ=255)                                                                                                                           ggggCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCtcgtcg                >  1:199507/1‑134 (MQ=255)                                                                                                                           ggggCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCTCGTCGCTATTc          >  1:215722/1‑140 (MQ=255)                                                                                                                           ggggCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCTCGTCGCTATTc          <  1:1073771/140‑1 (MQ=255)                                                                                                                           ggggCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCTCGTCGCTATTc          <  2:645775/140‑1 (MQ=255)                                                                                                                             ggCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCTCGTCGCTa             >  1:314616/1‑135 (MQ=255)                                                                                                                             ggCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCTCGTCGCTa             <  2:314616/135‑1 (MQ=255)                                                                                                                                gcgTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCTCGTCGCTATTCTatat     <  2:774908/140‑1 (MQ=255)                                                                                                                                 cgTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCTCGTCGCTa             >  2:422125/1‑131 (MQ=255)                                                                                                                                 cgTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCTCGTCGCTa             <  1:422125/131‑1 (MQ=255)                                                                                                                                 cgTTTATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCTCGTCGCTATTCTATATg    >  1:1043242/1‑140 (MQ=255)                                                                                                                                   tttATCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCTCGTCGCTATTCTATATggg  <  2:701068/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        TTCTCGCAAACTCAACGCTATAAGGCGTTGTTCTGGTTATCGTTATTTCATCTGCTGGTGATCACCTCCAGTAACTATCTGGTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCT‑GGGGCGCGTTTAGCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCTCGTCGCTATTCTATATGGG  >  NC_000913/3609226‑3609494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑