Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	444,317	G→T	L67I (CTC→ATC)	panE ←	2‑dehydropantoate 2‑reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	444,317	0	G	T	100.0%	280.6 / NA	80	L67I (CTC→ATC)	panE	2‑dehydropantoate 2‑reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (45/35);  total (45/35)

CTGCTGAATGTTTTGCAACTCTTCGATGGTGCCCATGCCGTTGTGAATTAACAGTATTGGCGTAGTTACAGGCAGTGTGGACGCGAGGCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGAGCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGATTCGTTAAATATCGAACCATCTGTCTCAACCAGATTCACGCTACAATAAGGTTGCGGTACGCGCAGCCAGCCCTGAACTTCATGAC  >  NC_000913/444183‑444448                                                                                                                                       |                                                                                                                                    ctgctgAATGTTTTGCAACTCTTCGATGGTGCCCATGCCGTTGTGAATTAACAGTATTGGCGTAGTTACAGGCAGTGTGGACGCGAGGCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGAt                                                                                                                                >  2:890540/1‑140 (MQ=255)  tgctgAATGTTTTGCAACTCTTCGATGGTGCCCATGCCGTTGTGAATTAACAGTATTGGCGTAGTTACAGGCAGTGTGGACGCGAGGCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATc                                                                                                                               >  1:787683/1‑140 (MQ=255)  tgctgAATGTTTTGCAACTCTTCGATGGTGCCCATGCCGTTGTGAATTAACAGTATTGGCGTAGTTACAGGCAGTGTGGACGCGAGGCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATc                                                                                                                               >  2:699481/1‑140 (MQ=255)   gctgAATGTTTTGCAACTCTTCGATGGTGCCCATGCCGTTGTGAATTAACAGTATTGGCGTAGTTACAGGCAGTGTGGACGCGAGGCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATCg                                                                                                                              >  2:632504/1‑140 (MQ=255)    ctgAATGTTTTGCAACTCTTCGATGGTGCCCATGCCGTTGTGAATTAACAGTATTGGCGTAGTTACAGGCAGTGTGGACGCGAGGCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATCGc                                                                                                                             >  1:817046/1‑140 (MQ=255)              tGCAACTCTTCGATGGTGCCCATGCCGTTGTGAATTAACAGTATTGGCGTAGTTACAGGCAGTGTGGACGCGAGGCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCgag                                                                                                                   <  2:676512/140‑1 (MQ=255)                 aaCTCTTCGATGGTGCCCATGCCGTTGTGAATTAACAGTATTGGCGTAGTTACAGGCAGTGTGGACGCGAGGCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGaaa                                                                                                                >  1:439167/1‑140 (MQ=255)                     ctTCGATGGTGCCCATGCCGTTGTGAATTAACAGTATTGGCGTAGTTACAGGCAGTGTGGACGCGAGGCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCg                                                                                                            >  2:957989/1‑140 (MQ=255)                     ctTCGATGGTGCCCATGCCGTTGTGAATTAACAGTATTGGCGTAGTTACAGGCAGTGTGGACGCGAGGCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCg                                                                                                            >  2:610572/1‑140 (MQ=255)                        cGATGGTGCCCATGCCGTTGTGAATTAACAGTATTGGCGTAGTTACAGGCAGTGTGGACGCGAGGCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGa                                                                                                         >  1:368116/1‑140 (MQ=255)                                   aTGCCGTTGTGAATTAACAGTATTGGCGTAGTTACAGGCAGTGTGGACGCGAGGCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGt                                                                                              <  2:817046/140‑1 (MQ=255)                                    tGCCGTTGTGAATTAACAGTATTGGCGTAGTTACAGGCAGTGTGGACGCGAGGCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTc                                                                                             >  2:591562/1‑140 (MQ=255)                                     gCCGTTGTGAATTAACAGTATTGGCGTAGTTACAGGCAGTGTGGACGCGAGGCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCa                                                                                            >  1:216055/1‑140 (MQ=255)                                       cGTTGTGAATTAACAGTATTGGCGTAGTTACAGGCAGTGTGGACGCGAGGCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGc                                                                                          <  1:890540/140‑1 (MQ=255)                                        gTTGTGAATTAACAGTATTGGCGTAGTTACAGGCAGTGTGGACGCGAGGCTTTTGACGGCATCGGAACCCTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCg                                                                                         <  1:610572/140‑1 (MQ=255)                                         ttGTGAATTAACAGTATTGGCGTAGTTACAGGCAGTGTGGACGCGAGGCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGa                                                                                        >  2:679218/1‑140 (MQ=255)                                          tgtgAATTAACAGTATTGGCGTAGTTACAGGCAGTGTGGACGCGAGGCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGAt                                                                                       >  2:826494/1‑140 (MQ=255)                                          tgtgAATTAACAGTATTGGCGTAGTTACAGGCAGTGTGGACGCGAGGCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGAt                                                                                       <  1:591562/140‑1 (MQ=255)                                              aaTTAACAGTATTGGCGTAGTTACAGGCAGTGTGGACGCGAGGCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGAt                                                                                       >  1:751876/1‑136 (MQ=255)                                              aaTTAACAGTATTGGCGTAGTTACAGGCAGTGTGGACGCGAGGCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGAt                                                                                       <  2:751876/136‑1 (MQ=255)                                                      gTATTGGCGTAGTTCCAGGCAGTGTGGACGCGAGGCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGAt                                                                                       <  1:907074/128‑1 (MQ=255)                                                      gTATTGGCGTAGTTACAGGCAGTGTGGACGCGAGGCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGAt                                                                                       >  2:907074/1‑128 (MQ=255)                                                        aTTGGCGTAGTTACAGGCAGTGTGGACGCGAGGCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGATTCGTTAAATATCGa                                                                         <  2:608984/140‑1 (MQ=255)                                                         ttGGCGTAGTTACAGGCAGTGTGGACGCGAGGCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGATTCGTTAAATATCGaa                                                                        >  1:127838/1‑140 (MQ=255)                                                             cGTAGTTACAGGCAGTGTGGACGCGAGGCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGATTCGTTTAATATCGAACCAt                                                                    <  2:542346/140‑1 (MQ=255)                                                             cGTAGTTACAGGCAGTGTGGACGCGAGGCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGATTCGTTAAATATCGAACCAt                                                                    <  1:679218/140‑1 (MQ=255)                                                                 gTTACAGGCAGTGTGGACGCGAGGCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGATTCGTTAAATATCGAACCATCTgg                                                                >  1:1021219/1‑139 (MQ=255)                                                                 gTTACAGGCAGTGTGGACGCGAGGCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGATTCGTTAAATATCGAACCATCTGt                                                                >  1:858374/1‑140 (MQ=255)                                                                 gTTACAGGCAGTGTGGACGCGAGGCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTAGGGGAGAGAAAAACGGAATCGTTGGCGGTCAGCCATTCGCTAAATTTCGAACCATCTga                                                                >  2:945424/1‑139 (MQ=255)                                                                       ggCAGTGTGGACGCGAGGCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGATTCGTTAAATATCGAACCATCTGTCTCAAc                                                          >  1:828038/1‑140 (MQ=255)                                                                          aGTGTGGACGCGAGGCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGATTCGTTAAATATCGAACCATCTGTCTCAACCAg                                                       <  2:659410/140‑1 (MQ=255)                                                                           gtgtGGACGCGAGGCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGATTCGTTAAATATCGAACCATCTGTCTCAACCAGa                                                      >  1:512449/1‑140 (MQ=255)                                                                            tgtgGACGCGAGGCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGATTCGTTAAATATCGAACCATCTGTCTCAAc                                                          <  1:523795/135‑1 (MQ=255)                                                                            tgtgGACGCGAGGCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGATTCGTTAAATATCGAACCATCTGTCTCAAc                                                          >  2:523795/1‑135 (MQ=255)                                                                            tgtgGACGCGAGGCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGATTCGTTAAATATCGAACCATCTGTCTCAACCAGAt                                                     >  1:61417/1‑140 (MQ=255)                                                                            tgtgGACGCGAGGCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGATTCGTTAAATATCGAACCATCTGTCTCAACCAGAt                                                     >  2:206838/1‑140 (MQ=255)                                                                            tgtgGACGCGAGGCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGATTCGTTAAATATCGAACCATCTGTCTCAACCAGAt                                                     >  1:258168/1‑140 (MQ=255)                                                                            tgtgGACGCGAGGCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGATTCGTTAAATATCGAACCATCTGTCTCAACCAGAt                                                     >  2:469758/1‑140 (MQ=255)                                                                            tgtgGACGCGAGGCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGATTCGTTAAATATCGAACCATCTGTCTCAACCAGAt                                                     >  1:452528/1‑140 (MQ=255)                                                                             gtgGACGCGAGGCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGATTCGTTAAATATCGAACCATCTGTCTCAACCAGAtt                                                    <  2:828038/140‑1 (MQ=255)                                                                             gtgGACGCGAGGCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGATTCGTTAAATATCGAACCATCTGTCTCAACCAGAtt                                                    <  2:787683/140‑1 (MQ=255)                                                                             gtgGACGCGAGGCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGATTCGTTAAATATCGAACCATCTGTCTCAACCAGAtt                                                    >  1:691870/1‑140 (MQ=255)                                                                             gtgGACGCGAGGCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGATTCGTTAAATATCGAACCATCTGTCTCAACCAGAtt                                                    <  2:61417/140‑1 (MQ=255)                                                                             gtgGACGCGAGGCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGATTCGTTAAATATCGAACCATCTGTCTCAACCAGAtt                                                    <  2:324153/140‑1 (MQ=255)                                                                              tgGACGCGAGGCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGATTCGTTAAATATCGAACCATCTGTCTCAACCAGATTc                                                   <  1:40050/140‑1 (MQ=255)                                                                              tgGACGCGAGGCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGATTCGTTAAATATCGAACCATCTGTCTCAACCAGATTc                                                   >  1:959874/1‑140 (MQ=255)                                                                               ggACGCGAGGCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGATTCGTTAAATATCGAACCATCTGTCTCAACCAGATTCa                                                  <  1:469758/140‑1 (MQ=255)                                                                               ggACGCGAGGCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGATTCGTTAAATATCGAACCATCTGTCTCAACCAGATTCa                                                  <  1:318563/140‑1 (MQ=255)                                                                                gACGCGAGGCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGATTCGTTAAATATCGAACCATCTGTCTCAACCAGATTCAc                                                 <  1:561259/140‑1 (MQ=255)                                                                                 aCGCGAGGCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGATTCGTTAAATATCGAACCATCTGTCTCAACCAGATTCACg                                                <  2:368116/140‑1 (MQ=255)                                                                                 aCGCGAGGCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGATTCGTTAAATATCGAACCATCTGTCTCAACCAGATTCACg                                                <  1:945424/140‑1 (MQ=255)                                                                                 aCGCGAGGCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGATTCGTTAAATATCGAACCATCTGTCTCAACCAGATTCACg                                                <  2:512449/140‑1 (MQ=255)                                                                                  cgcgAGGCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGATTCGTTAAATATCGAACCATCTGTCTCAACCAGATTCACGc                                               <  1:206838/140‑1 (MQ=255)                                                                                  cgcgAGGCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGATTCGTTAAATATCGAACCATCTGTCTCAACCAGATTCACGc                                               >  1:101888/1‑140 (MQ=255)                                                                                      aGGCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGATTCGTTAAATATCGAACCATCTGTCTCAACCGGATTCACGCTACa                                           >  1:823140/1‑140 (MQ=255)                                                                                      aGGCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGATTCGTTAAATATCGAACCATCTGTCTCAACCAGATTCACGCTACa                                           >  2:692810/1‑140 (MQ=255)                                                                                      aGGCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGATTCGTTAAATATCGAACCATCTGTCTCAACCAGATTCACGCTACa                                           >  1:726551/1‑140 (MQ=255)                                                                                      aGGCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGATTCGTTAAATATCGAACCATCTGTCTCAACCAGATTCACGCTACa                                           >  2:388914/1‑140 (MQ=255)                                                                                      aGGCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGATTCGTTAAATATCGAACCATCTGTCTCAACCAGATTCACGCTACa                                           >  2:919577/1‑140 (MQ=255)                                                                                      aGGCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGATTCGTTAAATATCGAACCATCTGTCTCAACCAGATTCACGCTACa                                           >  1:687604/1‑140 (MQ=255)                                                                                       ggCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGATTCGTTAAATATCGAACCATCTGTCTCAACCAGATTCACGCTACaa                                          <  2:726551/140‑1 (MQ=255)                                                                                        gCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGATTCGTTAAATATCGAACCATCTGTCTCAACCAGATTCAc                                                 >  2:443929/1‑132 (MQ=255)                                                                                        gCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGATTCGTTAAATATCGAACCATCTGTCTCAACCAGATTCAc                                                 <  1:443929/132‑1 (MQ=255)                                                                                        gCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGATTCGTTAAATATCGAACCATCTGTCTCAACCAGATTCACGCTACAAt                                         >  2:626200/1‑140 (MQ=255)                                                                                             tGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTTGCGAGAAAATCGGGATCGTTGGCGGTCAGCGATTCGTTAAATATCGAACCATCTGTCTCAACCAGATTCACg                                                <  2:591138/128‑1 (MQ=255)                                                                                             tGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTTGCGAGAAAATCGGGATCGTTGGCGGTCAGCGATTCGTTAAATATCGAACCATCTGTCTCAACCAGATTCACg                                                >  1:591138/1‑128 (MQ=255)                                                                                              gACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGATTCGTTAAATATCGAACCATCTGTCTCAACCAGATTCACGCTACAATAAGGtt                                   <  1:826494/140‑1 (MQ=255)                                                                                                            ccTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGATTCGTTAAATATCGAACCATCTGTCTCAACCAGATTCACGCTACAATAAGGTTGCGGTAcgcg                         <  1:92474/136‑1 (MQ=255)                                                                                                            ccTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGATTCGTTAAATATCGAACCATCTGTCTCAACCAGATTCACGCTACAATAAGGTTGCGGTAcgcg                         >  2:92474/1‑136 (MQ=255)                                                                                                            ccTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGATTCGTTAAATATCGAACCATCTGTCTCAACCAGATTCACGCTACAATAAGGTTGCGGTACGCGcagc                     <  1:919577/140‑1 (MQ=255)                                                                                                             cTGCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGATTCGTTAAATATCGAACCATCTGTCTCAACCGGATTCACGCTACAATAAGGTTGCGGTACGCGcagcc                    <  2:823140/140‑1 (MQ=255)                                                                                                               gCCATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGATTCGTTAAATATCGAACCATCTGTCTCAACCAGATTCACGCTACAATAAGGTTGCGGTACGCGcagccag                  >  2:234139/1‑140 (MQ=255)                                                                                                                 cATGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGATTCGTTAAATATCGAACCATCTGTCTCAACCAGATTCACGCTACAATAAGGTTGCGGTACGCGCagccagcc                <  1:388914/140‑1 (MQ=255)                                                                                                                  aTGCTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGATTCGTTAAATATCGAACCATCTGTCTCAACCAGATTCACGCTACAATAAGGTTGCGGTACGCGCAGCCAGccc               >  1:1045347/1‑140 (MQ=255)                                                                                                                     cTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGATTCGTTAAATATCGAACCATCTGTCTCAACCAGATTCACGCTACAATAAGGTTGCGGTACGCGCAGCCAGCCCTGa            >  2:591871/1‑140 (MQ=255)                                                                                                                     cTTTCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGATTCGTTAAATATCGAACCATCTGTCTCAACCAGATTCACGCTACAATAAGGTTGCGGTACGCGCAGCCAGCCCTGa            <  2:101888/140‑1 (MQ=255)                                                                                                                      tttCAGCGTCGCCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGATTCGTTAAATATCGAACCATCTGTCTCAACCAGATTCACGCTACAATAAGGTTGCGGTACGCGCAGCCAGCCCTGaa           <  1:692810/140‑1 (MQ=255)                                                                                                                      tttCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGATTCGTTAAATATCGAACCATCTGTCTCAACCAGATTCACGCTACAATAAGGTTGCGGTACGCGCAGCCAGCCCTGaa           <  2:784446/140‑1 (MQ=255)                                                                                                                       ttCAGCGTCACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGATTCGTTAAATATCGAACCATCTGTCTCAACCAGATTCACGCTACAATAAGGTTGCGGTACGCGCAGCCAGCCCTGAAc          >  1:897815/1‑140 (MQ=255)                                                                                                                               cACCAGGATCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGATTCGTTAAATATCGAACCATCTGTCTCAACCAGATTCACGCTACAATAAGGTTGCGGTACGCGCAGCCAGCCCTGAACTTCATGAc  >  2:483310/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                    CTGCTGAATGTTTTGCAACTCTTCGATGGTGCCCATGCCGTTGTGAATTAACAGTATTGGCGTAGTTACAGGCAGTGTGGACGCGAGGCTTTTGACGGCATCGGAAACCTGCCATGCTTTCAGCGTCACCAGGAGCAGATCGCTGGTGGCGAGAAAATCGGGATCGTTGGCGGTCAGCGATTCGTTAAATATCGAACCATCTGTCTCAACCAGATTCACGCTACAATAAGGTTGCGGTACGCGCAGCCAGCCCTGAACTTCATGAC  >  NC_000913/444183‑444448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑