Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,080,048	G→T	intergenic (+79/+251)	yiiF → / ← fdhE	protein YiiF/formate dehydrogenase formation protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,080,048	0	G	T	100.0%	299.8 / NA	85	intergenic (+79/+251)	yiiF/fdhE	protein YiiF/formate dehydrogenase formation protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (36/49);  total (36/49)

AGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGATGTGGTGGTGGGTAAATT  >  NC_000913/4079915‑4080178                                                                                                                                      |                                                                                                                                   aGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACa                                                                                                                              <  2:766154/140‑1 (MQ=255)       tgAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCg                                                                                                                        >  2:515817/1‑140 (MQ=255)        gAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGt                                                                                                                       >  2:663794/1‑140 (MQ=255)               aTGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACa                                                                                                                              <  1:511823/126‑1 (MQ=255)               aTGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACa                                                                                                                              >  2:511823/1‑126 (MQ=255)               aTGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAAtt                                                                                                                >  2:308306/1‑140 (MQ=255)               aTGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAAtt                                                                                                                >  1:34216/1‑140 (MQ=255)                 ggTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCa                                                                                                              <  2:782249/140‑1 (MQ=255)                   tgtCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCa                                                                                                              <  2:270678/138‑1 (MQ=255)                   tgtCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCa                                                                                                              >  1:270678/1‑138 (MQ=255)                   tgtCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAAt                                                                                                            >  2:177162/1‑140 (MQ=255)                   tgtCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAAt                                                                                                            >  2:287809/1‑140 (MQ=255)                     tCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGt                                                                                                          >  1:883242/1‑140 (MQ=255)                        aaTATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCa                                                                                                              <  1:86736/133‑1 (MQ=255)                        aaTATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCa                                                                                                              >  2:86736/1‑133 (MQ=255)                        aaTATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTAtt                                                                                                       >  2:755957/1‑140 (MQ=255)                        aaTATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTAtt                                                                                                       >  2:45781/1‑140 (MQ=255)                          tatCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGc                                                                                                     <  1:1016189/140‑1 (MQ=255)                            tCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAg                                                                                                   <  2:588269/140‑1 (MQ=255)                             cAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGt                                                                                                  >  1:688754/1‑140 (MQ=255)                               gCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGttt                                                                                                <  2:233899/140‑1 (MQ=255)                               gCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGttt                                                                                                <  2:654203/140‑1 (MQ=255)                                 gTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTAt                                                                                              >  1:832413/1‑140 (MQ=255)                                  tAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTAtt                                                                                             >  2:434265/1‑140 (MQ=255)                                  tAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCGTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTAtt                                                                                             >  2:866216/1‑140 (MQ=255)                                    aGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTgg                                                                                           <  2:528857/140‑1 (MQ=255)                                    aGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTgg                                                                                           <  1:23635/140‑1 (MQ=255)                                    aGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTgg                                                                                           >  2:23635/1‑140 (MQ=255)                                    aGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTgg                                                                                           <  1:287809/140‑1 (MQ=255)                                    aGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTgg                                                                                           <  1:308306/140‑1 (MQ=255)                                    aGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTgg                                                                                           <  1:849026/140‑1 (MQ=255)                                    aGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTgg                                                                                           <  2:832413/140‑1 (MQ=255)                                    aGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTgg                                                                                           <  1:434265/140‑1 (MQ=255)                                    aGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCGTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTgg                                                                                           <  1:866216/140‑1 (MQ=255)                                      cTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTa                                                                                         <  2:892280/140‑1 (MQ=255)                                       tGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAg                                                                                        <  1:177162/140‑1 (MQ=255)                                       tGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAg                                                                                        >  2:617566/1‑140 (MQ=255)                                         cgcgAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGc                                                                                      >  1:337823/1‑140 (MQ=255)                                          gcgAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGcc                                                                                     >  1:272504/1‑140 (MQ=255)                                            gAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTgg                                                                                           >  1:257591/1‑132 (MQ=255)                                            gAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTgg                                                                                           <  2:257591/132‑1 (MQ=255)                                                   ggTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAagcg                                                                            <  2:337823/140‑1 (MQ=255)                                                     tAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAagcgca                                                                          <  2:836908/140‑1 (MQ=255)                                                     tAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAagcgca                                                                          <  1:609299/140‑1 (MQ=255)                                                          ggAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAgcgcagcgca                                                                     >  2:1050169/1‑140 (MQ=255)                                                          ggAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAgcgcagcgca                                                                     <  1:1050169/140‑1 (MQ=255)                                                            aaaaaaTGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATc                                                                   <  1:963644/140‑1 (MQ=255)                                                             aaaaaTGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCa                                                                  >  2:287692/1‑140 (MQ=255)                                                               aaaTGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAgg                                                                <  1:540328/140‑1 (MQ=255)                                                               aaaTGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAgg                                                                <  1:184102/140‑1 (MQ=255)                                                               aaaTGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAgg                                                                <  1:832813/140‑1 (MQ=255)                                                                           tCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCt                                                    <  2:272504/140‑1 (MQ=255)                                                                               aTTGCTGACAACGTGTGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTa                                                        >  1:189390/1‑132 (MQ=255)                                                                               aTTGCTGACAACGTGTGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTa                                                        <  2:189390/132‑1 (MQ=255)                                                                                 tGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGtcat                                              <  2:350564/140‑1 (MQ=255)                                                                                  gCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGtcatc                                             <  2:883242/140‑1 (MQ=255)                                                                                   cTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGtcatca                                            >  2:1035230/1‑140 (MQ=255)                                                                                         aCGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCa                                      >  1:945768/1‑140 (MQ=255)                                                                                         aCGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCa                                      <  2:649905/140‑1 (MQ=255)                                                                                         aCGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAAGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCa                                      >  2:269322/1‑140 (MQ=255)                                                                                            tGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGc                                   >  1:902270/1‑140 (MQ=255)                                                                                             gcgcGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGcc                                  >  2:37167/1‑140 (MQ=255)                                                                                             gcgcGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGcc                                  >  2:742366/1‑140 (MQ=255)                                                                                             gcgcGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGcc                                  >  1:1000520/1‑140 (MQ=255)                                                                                             gcgcGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGcc                                  >  2:559726/1‑140 (MQ=255)                                                                                               gcgTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCa                                <  2:897960/140‑1 (MQ=255)                                                                                                cgTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCAt                               <  2:902270/140‑1 (MQ=255)                                                                                                  ttgttCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATaa                             <  1:287692/140‑1 (MQ=255)                                                                                                  ttgttCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATaa                             <  2:34216/140‑1 (MQ=255)                                                                                                  ttgttCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATaa                             <  1:515817/140‑1 (MQ=255)                                                                                                  ttgttCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATaa                             <  2:246566/140‑1 (MQ=255)                                                                                                     ttCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCt                          <  2:512385/140‑1 (MQ=255)                                                                                                     ttCATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCt                          <  1:617566/140‑1 (MQ=255)                                                                                                        aTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTAt                       <  1:1035230/140‑1 (MQ=255)                                                                                                             ggATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGAtg                  <  2:502964/140‑1 (MQ=255)                                                                                                             ggATGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATCGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGAtg                  <  2:339829/140‑1 (MQ=255)                                                                                                               aTGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGAtgtg                >  2:231061/1‑140 (MQ=255)                                                                                                               aTGCGGCGTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGAtgtg                >  1:238131/1‑140 (MQ=255)                                                                                                                   ggcgTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGATgtggtgg            <  1:559726/140‑1 (MQ=255)                                                                                                                   ggcgTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGATgtggtgg            <  1:45781/140‑1 (MQ=255)                                                                                                                    gcgTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGAtg                  >  1:562561/1‑133 (MQ=255)                                                                                                                    gcgTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGAtg                  <  2:562561/133‑1 (MQ=255)                                                                                                                     cgTGAACGCCTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGATgtggtggtg          <  2:238131/140‑1 (MQ=255)                                                                                                                             ccTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGATGTGGTGGTGGGTAAAtt  >  2:146341/1‑140 (MQ=255)                                                                                                                             ccTTATCCGTCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGATGTGGTGGTGGGTAAAtt  <  1:945663/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                   AGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGATGTGGTGGTGGGTAAATT  >  NC_000913/4079915‑4080178

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑