Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,143,491	C→A	A321D (GCT→GAT)	frwC →	putative PTS enzyme IIC component FrwC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,143,491	0	C	A	100.0%	214.8 / NA	62	A321D (GCT→GAT)	frwC	putative PTS enzyme IIC component FrwC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (36/26);  total (36/26)

CTTCCATCATGGTCGGTTCAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAACGAGCCA  >  NC_000913/4143359‑4143624                                                                                                                                     |                                                                                                                                      cttccATCATGGTCGGTTCAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGATGTCGGa                                                                                                                                <  1:373546/140‑1 (MQ=255) cttccATCATGGTCGGTTCAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGATGTCGGa                                                                                                                                >  1:301354/1‑140 (MQ=255)   tccATCATGGTCGGTTCAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGATGTCGGAGc                                                                                                                              >  2:614324/1‑140 (MQ=255)           ggTCGGTTCAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGATGTCGGAGCAGTGGTGa                                                                                                                      >  1:482192/1‑140 (MQ=255)           ggTCGGTTCAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGATGTCGGAGCAGTGGTGa                                                                                                                      >  1:625595/1‑140 (MQ=255)            gTCGGTTCAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGATGTCGGAGCAgtggtg                                                                                                                       <  1:503671/138‑1 (MQ=255)            gTCGGTTCAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGATGTCGGAGCAgtggtg                                                                                                                       >  2:503671/1‑138 (MQ=255)            gTCGGTTCAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGATGTCGGAGCAGTGGTGAc                                                                                                                     <  1:1059938/140‑1 (MQ=255)                gTTCAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGATGTCGGAGCAGTGGTGACGGCt                                                                                                                 >  2:1065077/1‑140 (MQ=255)                 ttCAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGATGTCGGAGCAGTGGTGACGGCTg                                                                                                                <  2:301354/140‑1 (MQ=255)                 ttCAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGATGTCGGAGCAGTGGTGACGGCTg                                                                                                                <  1:733997/140‑1 (MQ=255)                  tCAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGATGTCGGAg                                                                                                                               <  2:477449/124‑1 (MQ=255)                  tCAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGATGTCGGAg                                                                                                                               >  1:477449/1‑124 (MQ=255)                  tCAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGATGTCGGAGCAGTGGTGACGGCTGt                                                                                                               >  2:157138/1‑140 (MQ=255)                           ggtgCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGATGTCGGAGCAGTGGTGACGGCTGTTTGTGTGaa                                                                                                      >  1:55143/1‑140 (MQ=255)                             tgCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGATGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACg                                                                                                    <  2:625595/140‑1 (MQ=255)                                aGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGATGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGc                                                                                                 >  1:631962/1‑140 (MQ=255)                                 gTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGATGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCt                                                                                                <  2:55143/140‑1 (MQ=255)                                  tAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGATGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTg                                                                                               >  2:963251/1‑140 (MQ=255)                                   aaCTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGATGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGa                                                                                              <  1:157138/140‑1 (MQ=255)                                       gcggcgCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGATGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAg                                                                                          >  2:500616/1‑140 (MQ=255)                                       gcggcgCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGATGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAg                                                                                          >  2:103642/1‑140 (MQ=255)                                       gcggcgCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGATGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAg                                                                                          >  2:811336/1‑140 (MQ=255)                                          gcgcTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGATGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAAc                                                                                                     <  1:453353/126‑1 (MQ=255)                                          gcgcTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGATGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAAc                                                                                                     >  2:453353/1‑126 (MQ=255)                                                       cgcAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGATGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAAt                                                                          <  1:951367/140‑1 (MQ=255)                                                       cgcAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGATGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAAt                                                                          <  1:486734/140‑1 (MQ=255)                                                            tGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGATGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTc                                                                     >  1:783442/1‑140 (MQ=255)                                                              cTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGATGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCtt                                                                   >  1:1011763/1‑140 (MQ=255)                                                                 tGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGATGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGa                                                                <  1:811336/140‑1 (MQ=255)                                                                  gCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGATGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGAc                                                               <  1:1065077/140‑1 (MQ=255)                                                                      gCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGATGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGa                                                                <  2:167215/135‑1 (MQ=255)                                                                      gCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGATGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGa                                                                >  1:167215/1‑135 (MQ=255)                                                                      gCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGATGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACtgat                                                           >  2:1009951/1‑140 (MQ=255)                                                                      gCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGATGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACtgat                                                           >  2:517002/1‑140 (MQ=255)                                                                        tGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGATGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACtgatga                                                         >  1:761293/1‑140 (MQ=255)                                                                           ggtggtCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGATGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGaaaa                                                      >  2:1006824/1‑140 (MQ=255)                                                                           ggtggtCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGATGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGaaaa                                                      >  1:662125/1‑140 (MQ=255)                                                                                tCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGATGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAaagaag                                                 >  1:138581/1‑140 (MQ=255)                                                                                 cTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGATGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAagaaga                                                <  2:631962/140‑1 (MQ=255)                                                                                 cTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGATGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAagaaga                                                <  1:1009951/140‑1 (MQ=255)                                                                                     ttGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGATGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAgacgac                                            <  1:103642/140‑1 (MQ=255)                                                                                          ctgcCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGATGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCtggg                                       >  2:424473/1‑139 (MQ=255)                                                                                          ctgcCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGATGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCtgga                                       >  1:157208/1‑140 (MQ=255)                                                                                             ccGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGATGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAagaaga                                                >  1:739928/1‑128 (MQ=255)                                                                                             ccGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGATGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAagaaga                                                <  2:739928/128‑1 (MQ=255)                                                                                              cGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGATGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCtggaattg                                   >  2:29156/1‑140 (MQ=255)                                                                                                  ggtTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGATGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCtggatttggatt                               >  2:896555/1‑140 (MQ=255)                                                                                                    ttGAAGGCAAGCTGGGTTATATCGCAGCAGTGGATGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGAtttt                             <  2:1011763/140‑1 (MQ=255)                                                                                                          gCAAGCTGGGTTATATCGCAGCAGTGGATGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAaatt                       >  1:1032918/1‑140 (MQ=255)                                                                                                            aaGCTGGGTTATATCGCAGCAGTGGATGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAaattaa                     >  2:21929/1‑140 (MQ=255)                                                                                                             aGCTGGGTTATATCGCAGCAGTGGATGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAaattaat                    <  1:614324/140‑1 (MQ=255)                                                                                                             aGCTGGGTTATATCGCAGCAGTGGATGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAaattaat                    <  1:21929/140‑1 (MQ=255)                                                                                                             aGCTGGGTTATATCGCAGCAGTGGATGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAaattaat                    <  2:761293/140‑1 (MQ=255)                                                                                                             aGCTGGGTTATATCGCAGCAGTGGATGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAaattaat                    <  1:1006824/140‑1 (MQ=255)                                                                                                             aGCTGGGTTATATCGCAGCAGTGGATGACGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAaattaat                    >  1:916861/1‑140 (MQ=255)                                                                                                             aGCTGGGTTATATCGCAGCAGTGGATGACGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAaattaat                    <  2:916861/140‑1 (MQ=255)                                                                                                                 gggTTATATCGCAGCAGTGGATGTCGGCGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAaattaattaat                >  1:712524/1‑140 (MQ=255)                                                                                                                            cagcagTGGATGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAACGAg     >  1:535922/1‑140 (MQ=255)                                                                                                                            cagcagTGGATGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAACGAg     >  1:591225/1‑140 (MQ=255)                                                                                                                             agcagTGGATGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAACGAGc    >  1:427279/1‑140 (MQ=255)                                                                                                                               cagTGGATGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAACGAGCCa  <  1:209221/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                      CTTCCATCATGGTCGGTTCAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAACGAGCCA  >  NC_000913/4143359‑4143624

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑