Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	652,520	G→T	D96Y (GAT→TAT)	dpiB →	sensory histidine kinase DpiB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	652,520	0	G	T	100.0%	286.6 / NA	82	D96Y (GAT→TAT)	dpiB	sensory histidine kinase DpiB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (35/47);  total (35/47)

TTCATGTACGCGACATGGCAATGAATCAGGCGAAAATTATTGCCTCCAATGACAGTGTCATCTCTGCGGTGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGG  >  NC_000913/652386‑652653                                                                                                                                       |                                                                                                                                      ttCATGTACGCGACATGGCAATGAATCAGGCGAAAATTATTGCCTCCAATGACAGTGTCATCTCTGCGGTGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTtattat                                                                                                                                  >  2:623189/1‑140 (MQ=255)   cATGTACGCGACATGGCAATGAATCAGGCGAAAATTATTGCCTCCAATGACAGTGTCATCTCTGCGGTGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTAtgt                                                                                                                                <  2:733558/140‑1 (MQ=255)   cATGTACGCGACATGGCAATGAATCAGGCGAAAATTATTGCCTCCAATGACAGTGTCATCTCTGCGGTGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTAtgt                                                                                                                                >  1:156620/1‑140 (MQ=255)   cATGTACGCGACATGGCAATGAATCAGGCGAAAATTATTGCCTCCAATGACAGTGTCATCTCTGCGGTGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTAtgt                                                                                                                                >  2:139435/1‑140 (MQ=255)   cATGTACGCGACATGGCAATGAATCAGGCGAAAATTATTGCCTCCAATGACAGTGTCATCTCTGCGGTGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTAtgt                                                                                                                                >  1:3416/1‑140 (MQ=255)     tGTACGCGACATGGCAATGAATCAGGCGAAAATTATTGCCTCCAATGACAGTGTCATCTCTGCGGTGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTATgtgg                                                                                                                              <  1:139435/140‑1 (MQ=255)           cgACATGGCAATGAATCAGGCGAAAATTATTGCCTCCAATGACAGTGTCATCTCTGCGGTGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTAtt                                                                                                                                    >  1:1049700/1‑128 (MQ=255)           cgACATGGCAATGAATCAGGCGAAAATTATTGCCTCCAATGACAGTGTCATCTCTGCGGTGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTAtt                                                                                                                                    <  2:1049700/128‑1 (MQ=255)             aCATGGCAATGAATCAGGCGAAAATTATTGCCTCCAATGACAGTGTCATCTCTGCGGTGAAAACGCATGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTATGTGGTGATTggg                                                                                                                      >  1:213038/1‑140 (MQ=255)               aTGGCAATGAATCAGGCGAAAATTATTGCCTCCAATGACAGTGTCATCTCTGCGGTGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTAtg                                                                                                                                 <  2:42443/127‑1 (MQ=255)               aTGGCAATGAATCAGGCGAAAATTATTGCCTCCAATGACAGTGTCATCTCTGCGGTGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTAtg                                                                                                                                 >  1:42443/1‑127 (MQ=255)               aTGGCAATGAATCAGGCGAAAATTATTGCCTCCAATGACAGTGTCATCTCTGCGGTGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGa                                                                                                                    >  2:303323/1‑140 (MQ=255)                 ggCAATGAATCAGGCGAAAATTATTGCCTCCAATGACAGTGTCATCTCTGCGGTGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGAcc                                                                                                                  >  2:931273/1‑140 (MQ=255)                  gCAATGAATCAGGCGAAAATTATTGCCTCCAATGACAGTGTCATCTCTGCGGTGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCg                                                                                                                 >  1:25442/1‑140 (MQ=255)                           cAGGCGAAAATTATTGCCTCCAATGACAGTGTCATCTCTGCGGTGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGAt                                                                                                        >  1:987959/1‑140 (MQ=255)                           cAGGCGAAAATTATTGCCTCCAATGACAGTGTCATCTCTGCGGTGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGAt                                                                                                        <  2:122832/140‑1 (MQ=255)                           cAGGCGAAAATTATTGCCTCCAATGACAGTGTCATCTCTGCGGTGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGAt                                                                                                        <  2:288928/140‑1 (MQ=255)                           cAGGCGAAAATTATTGCCTCCAATGACAGTGTCATCTCTGCGGTGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGAt                                                                                                        <  2:3416/140‑1 (MQ=255)                           cAGGCGAAAATTATTGCCTCCAATGACAGTGTCATCTCTGCGGTGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGAt                                                                                                        <  2:560303/140‑1 (MQ=255)                           cAGGCGAAAATTATTGCCTCCAATGACAGTGTCATCTCTGCGGTGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGAt                                                                                                        <  1:303323/140‑1 (MQ=255)                            aGGCGAAAATTATTGCCTCCAATGACAGTGTCATCTCTGCGGTGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGATc                                                                                                       >  1:508371/1‑140 (MQ=255)                              gCGAAAATTATTGCCTCCAATGACAGTGTCATCTCTGCGGTGAAAACCGGCGACTACAAACGGCTGGCGCCCATCGCTAACAAATTACAAAGAGATAGCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGATCCg                                                                                                     <  2:508780/140‑1 (MQ=255)                                     ttattGCCTCCAATGACAGTGTCATCTCTGCGGTGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTAc                                                                                              <  2:987959/140‑1 (MQ=255)                                      tattGCCTCCAATGACAGTGTCATCTCTGCGGTGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTAcc                                                                                             <  2:610428/140‑1 (MQ=255)                                             tCCAATGACAGTGTCATCTCTGCGGTGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGAt                                                                                                        <  2:762516/122‑1 (MQ=255)                                             tCCAATGACAGTGTCATCTCTGCGGTGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGAt                                                                                                        >  1:762516/1‑122 (MQ=255)                                                        tgtCATCTCTGCGGTGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAGTTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGaaa                                                                            <  1:552590/139‑1 (MQ=255)                                                        tgtCATCTCTGCGGTGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAGTTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGaaa                                                                            >  2:552590/1‑139 (MQ=255)                                                        tgtCATCTCTGCGGTGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCgg                                                                                 >  2:228107/1‑134 (MQ=255)                                                        tgtCATCTCTGCGGTGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCgg                                                                                 <  1:228107/134‑1 (MQ=255)                                                        tgtCATCTCTGCGGTGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGaaaa                                                                           >  1:782068/1‑140 (MQ=255)                                                        tgtCATCTCTGCGGTGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGaaaa                                                                           >  1:850861/1‑140 (MQ=255)                                                        tgtCATCTCTGCGGTGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGaaaa                                                                           >  1:537221/1‑140 (MQ=255)                                                        tgtCATCTCTGCGGTGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGaaaa                                                                           >  1:671029/1‑140 (MQ=255)                                                        tgtCATCTCTGCGGTGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGaaaa                                                                           >  1:144722/1‑140 (MQ=255)                                                         gtCATCTCTGCGGTGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAAt                                                                          >  1:769524/1‑140 (MQ=255)                                                         gtCATCTCTGCGGTGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAAt                                                                          >  1:1013642/1‑140 (MQ=255)                                                         gtCATCTCTGCGGTGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAAt                                                                          >  2:520825/1‑140 (MQ=255)                                                         gtCATCTCTGCGGTGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAAt                                                                          >  1:305287/1‑140 (MQ=255)                                                          tCATCTCTGCGGTGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAAtt                                                                         >  2:783693/1‑140 (MQ=255)                                                          tCATCTCTGCGGTGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAAtt                                                                         >  1:535180/1‑140 (MQ=255)                                                            aTCTCTGCGGTGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTgg                                                                       <  1:331158/140‑1 (MQ=255)                                                            aTCTCTGCGGTGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTgg                                                                       <  1:32730/140‑1 (MQ=255)                                                            aTCTCTGCGGTGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTgg                                                                       <  2:782068/140‑1 (MQ=255)                                                              ctctGCGGTGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGtt                                                                     <  2:769524/140‑1 (MQ=255)                                                              ctctGCGGTGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGtt                                                                     <  1:756973/140‑1 (MQ=255)                                                                ctGCGGTGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTgg                                                                       >  2:410689/1‑136 (MQ=255)                                                                ctGCGGTGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTgg                                                                       <  1:410689/136‑1 (MQ=255)                                                                 tGCGGTGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTgg                                                                       >  1:849760/1‑135 (MQ=255)                                                                 tGCGGTGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTgg                                                                       <  2:849760/135‑1 (MQ=255)                                                                 tGCGGTGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATc                                                                  >  2:751191/1‑140 (MQ=255)                                                                 tGCGGTGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATc                                                                  <  2:581066/140‑1 (MQ=255)                                                                      tGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATg                                                             <  2:850861/140‑1 (MQ=255)                                                                      tGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATg                                                             <  1:623189/140‑1 (MQ=255)                                                                      tGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTATGTGGGGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATg                                                             <  2:273920/140‑1 (MQ=255)                                                                        aaaaCGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCa                                                           <  1:7774/140‑1 (MQ=255)                                                                        aaaaCGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCa                                                           <  1:783693/140‑1 (MQ=255)                                                                        aaaaCGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCa                                                           <  2:1037809/140‑1 (MQ=255)                                                                          aaCGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGt                                                         <  2:1038382/140‑1 (MQ=255)                                                                           aCGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTgg                                                                       <  1:313951/125‑1 (MQ=255)                                                                           aCGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTgg                                                                       >  2:313951/1‑125 (MQ=255)                                                                                      cAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAgg                                             >  2:872115/1‑140 (MQ=255)                                                                                      cAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAgg                                             <  1:979337/140‑1 (MQ=255)                                                                                       aaaCGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCa                                                           <  1:507020/125‑1 (MQ=255)                                                                                       aaaCGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCa                                                           >  2:507020/1‑125 (MQ=255)                                                                                           ggCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGgcgcg                                        <  2:144722/140‑1 (MQ=255)                                                                                             cTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCt                                      >  1:318760/1‑140 (MQ=255)                                                                                             cTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCt                                      >  2:1014519/1‑140 (MQ=255)                                                                                             cTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCt                                      <  2:238813/140‑1 (MQ=255)                                                                                               ggCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTgg                                    <  2:508371/140‑1 (MQ=255)                                                                                                  gACCATCGCTAACAAATTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGgaga                                 <  2:535180/140‑1 (MQ=255)                                                                                                       tCGCTAACAAATTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAggg                            <  1:520825/140‑1 (MQ=255)                                                                                                           tAACAAATTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGgaga                        <  2:305287/140‑1 (MQ=255)                                                                                                            aaCAAATTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGagag                       <  1:931273/140‑1 (MQ=255)                                                                                                             aCAAATTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGc                      <  2:318760/140‑1 (MQ=255)                                                                                                              cAAATTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCt                     <  1:1014519/140‑1 (MQ=255)                                                                                                                 aTTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACt                  >  2:709265/1‑140 (MQ=255)                                                                                                                 aTTACAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACt                  >  2:799652/1‑140 (MQ=255)                                                                                                                    aCAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTTGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCa               <  2:159604/140‑1 (MQ=255)                                                                                                                    aCAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCa               <  2:671029/140‑1 (MQ=255)                                                                                                                    aCAAAGAGATACCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGATCCACCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCa               <  2:20327/140‑1 (MQ=255)                                                                                                                              aCCGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGaa     <  2:213038/140‑1 (MQ=255)                                                                                                                               ccGATTTTTATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGaaa    <  2:537221/140‑1 (MQ=255)                                                                                                                                 gATTTTTATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAgg  <  1:799652/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                      TTCATGTACGCGACATGGCAATGAATCAGGCGAAAATTATTGCCTCCAATGACAGTGTCATCTCTGCGGTGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGG  >  NC_000913/652386‑652653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑