Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,035,902	G→T	G45C (GGT→TGT)	hyaD →	putative hydrogenase 1 maturation protease HyaD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,035,902	0	G	T	100.0%	154.8 / NA	44	G45C (GGT→TGT)	hyaD	putative hydrogenase 1 maturation protease HyaD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (24/20);  total (24/20)

TCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCG  >  NC_000913/1035768‑1036037                                                                                                                                       |                                                                                                                                        tCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCTGTACt                                                                                                                                    >  2:254694/1‑140 (MQ=255)  cATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCTGTACTc                                                                                                                                   <  1:357389/140‑1 (MQ=255)            cAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCAGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCTGTACTCAGGGACTGaa                                                                                                                         <  1:904325/140‑1 (MQ=255)                  gtggtgGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCTGTACTCAGGGACTGAACTTGCt                                                                                                                   <  2:60567/140‑1 (MQ=255)                             ggggCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCTGTACTCAGGGACTGAACTTGCTGGGGTATGTCg                                                                                                        >  1:453124/1‑140 (MQ=255)                                   gggCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCTGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAgcg                                                                                                  >  2:790894/1‑140 (MQ=255)                                    ggCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCTGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAgcgc                                                                                                 <  1:377074/140‑1 (MQ=255)                                      cAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCTGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCgcca                                                                                               <  2:885887/140‑1 (MQ=255)                                       aaCCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCTGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCgccag                                                                                              <  1:790894/140‑1 (MQ=255)                                           tgctgTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCTGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCAt                                                                                          <  2:390016/140‑1 (MQ=255)                                              tgtgGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCTGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTg                                                                                       >  2:383597/1‑140 (MQ=255)                                              tgtgGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCTGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTg                                                                                       >  2:151380/1‑140 (MQ=255)                                                   gCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCTGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGAt                                                                                  >  1:196979/1‑140 (MQ=255)                                                       aTGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCTGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATtctc                                                                              >  1:502014/1‑140 (MQ=255)                                                       aTGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCTGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATtctc                                                                              >  1:270083/1‑140 (MQ=255)                                                              cTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCTGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCa                                                                       <  2:196979/140‑1 (MQ=255)                                                                      tGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCTGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTAc                                                               <  2:270083/140‑1 (MQ=255)                                                                            tGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCTGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGctg                                                         <  2:836799/140‑1 (MQ=255)                                                                                            tGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCTGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGc                                         >  1:637678/1‑140 (MQ=255)                                                                                                 aTTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCTGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAAcc                                    >  2:580535/1‑140 (MQ=255)                                                                                                    aCCACTGGCCCGAGTATGTGGAGATTGTCGATGGCTGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTAt                                 >  1:57845/1‑140 (MQ=255)                                                                                                        cTGGCCCGAGTATGTGGAGATTGTCGATGGCTGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCg                             >  1:755530/1‑140 (MQ=255)                                                                                                         tGGCCCGAGTATGTGGAGATTGTCGATGGCTGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCgg                            >  1:832262/1‑140 (MQ=255)                                                                                                         tGGCCCGAGTATGTGGAGATTGTCGATGGCTGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCgg                            >  2:487251/1‑140 (MQ=255)                                                                                                             ccGAGTATGTGGAGATTGTCGATGGCTGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGaa                        >  1:534291/1‑140 (MQ=255)                                                                                                                aGTATGTGGAGATTGTCGATGGCTGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGc                     >  2:210390/1‑140 (MQ=255)                                                                                                                  tATGTGGAGATTGTCGATGGCTGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCAt                   <  2:832262/140‑1 (MQ=255)                                                                                                                    tgtgGAGATTGTCGATGGCTGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGTAGAACGCATTc                 <  2:46449/140‑1 (MQ=255)                                                                                                                    tgtgGAGATTGTCGATGGCTGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTc                 <  2:502014/140‑1 (MQ=255)                                                                                                                    tgtgGAGATTGTCGATGGCTGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTc                 <  2:755530/140‑1 (MQ=255)                                                                                                                    tggggAGATTGTCGATGGCTGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGGCTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTc                 <  2:650144/137‑1 (MQ=255)                                                                                                                     gtgGAGATTGTCGATGGCTGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTcc                >  1:708460/1‑140 (MQ=255)                                                                                                                      tgGAGATTGTCGATGGCTGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCg               <  2:903103/140‑1 (MQ=255)                                                                                                                        gagaTTGTCGATGGCTGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGc             <  2:225196/140‑1 (MQ=255)                                                                                                                           aTTGTCGATGGCTGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTa          >  2:827018/1‑140 (MQ=255)                                                                                                                            ttGTCGATGGCTGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTc                 >  2:825749/1‑132 (MQ=255)                                                                                                                            ttGTCGATGGCTGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTc                 <  1:825749/132‑1 (MQ=255)                                                                                                                              gTCGATGGCTGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTAtct       >  2:662006/1‑140 (MQ=255)                                                                                                                              gTCGATGGCTGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTAtct       >  2:725545/1‑140 (MQ=255)                                                                                                                              gTCGATGGCTGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTAtct       >  2:154740/1‑140 (MQ=255)                                                                                                                               tCGATGGCTGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTAC‑GGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCa     >  1:1037751/1‑140 (MQ=255)                                                                                                                                 gATGGCTGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAg    <  2:595460/140‑1 (MQ=255)                                                                                                                                   tGGCTGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGc             <  1:978959/129‑1 (MQ=255)                                                                                                                                   tGGCTGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGc             >  2:978959/1‑129 (MQ=255)                                                                                                                                   tGGCTGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAgcg  <  1:827018/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        TCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCG  >  NC_000913/1035768‑1036037

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑