Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,510,229	C→A	A76E (GCG→GAG)	ydcR →	fused putative DNA‑binding transcriptional regulator/putative aminotransferase YdcR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,510,229	0	C	A	100.0%	116.5 / NA	34	A76E (GCG→GAG)	ydcR	fused putative DNA‑binding transcriptional regulator/putative aminotransferase YdcR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (18/16);  total (18/16)

ACCAGGTGGCGCTTTCAGGCATGAGCTTTATGACTGTCAGCCATGCCTATCAGTTGCTCGAAAGTCAGGGATATATTATCGCACGACCGCAGTCGGGTTATTACGTTGCGCCACAGGCAATAAAAATGCCGAAAGCGCCAGTCATTCCAGTCACTCGAGATGAAGCAGTCGATATCAACACTTATATTTTTGATATGTTGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTTCCCGACCCGCGACTTTTCCCCCTCC  >  NC_000913/1510094‑1510363                                                                                                                                        |                                                                                                                                       aCCAGGTGGCGCTTTCAGGCATGAGCTTTATGACTGTCAGCCATGCCTATCAGTTGCTCGAAAGTCAGGGATATATTATCGCACGACCGCAGTCGGGTTATTACGTTGCGCCACAGGCAATAAAAATGCCGAAAGAGCCa                                                                                                                                    <  2:351140/140‑1 (MQ=255)   cAGGTGGCGCTTTCAGGCATGAGCTTTATGACTGTCAGCCATGCCTATCAGTTGCTCGAAAGTCAGGGATATATTATCGCACGACCGCAGTCGGGTTATTACGTTGCGCCACAGGCAATAAAAATGCCGAAAGAGCCAGt                                                                                                                                  <  2:278260/140‑1 (MQ=255)            cTTTCAGGCATGAGCTTTATGACTGTCAGCCATGCCTATCAGTTGCTCGAAAGTCAGGGATATATTATCGCACGACCGCAGTCGGGTTATTACGTTGCGCCACAGGCAATAAAAATGCCGAAAGAGCCAGTCATTCCAGt                                                                                                                         <  2:11113/140‑1 (MQ=255)                        aGCTTTATGACTGTCAGCCATGCCTATCAGTTGCTCGAAAGTCAGGGATATATTATCGCACGACCGCAGTCGGGTTATTACGTTGCGCCACAGGCAATAAAAATGCCGAAAGAGCCAGTCATTCCAGTCACTCGAGATGa                                                                                                             >  1:237374/1‑140 (MQ=255)                        aGCTTTATGACTGTCAGCCATGCCTATCAGTTGCTCGAAAGTCAGGGATATATTATCGCACGACCGCAGTCGGGTTATTACGTTGCGCCACAGGCAATAAAAATGCCGAAAGAGCCAGTCATTCCAGTCACTCGAGATGa                                                                                                             >  2:958190/1‑140 (MQ=255)                               tGACTGTCAGCCATGCCTATCAGTTGCTCGAAAGTCAGGGATATATTATCGCACGACCGCAGTCGGGTTATTACGTTGCGCCACAGGCAATAAAAATGCCGAAAGAGCCAGTCATTCCAGTCACTCGAGATGAAGCAGTc                                                                                                      <  1:1056119/140‑1 (MQ=255)                               tGACTGTCAGCCATGCCTATCAGTTGCTCGAAAGTCAGGGATATATTATCGCACGACCGCAGTCGGGTTATTACGTTGCGCCACAGGCAATAAAAATGCCGAAAGAGCCAGTCATTCCAGTCACTCGAGATGAAGCAGTc                                                                                                      <  2:918549/140‑1 (MQ=255)                                             gCCTATCAGTTGCTCGAAAGTCAGGGATATATTATCGCACGACCGCAGTCGGGTTATTACGTTGCGCCACAGGCAATAAAAATGCCGAAAGAGCCAGTCATTCCAGTCACTCGAGATGAAGCAGTc                                                                                                      >  2:443987/1‑126 (MQ=255)                                             gCCTATCAGTTGCTCGAAAGTCAGGGATATATTATCGCACGACCGCAGTCGGGTTATTACGTTGCGCCACAGGCAATAAAAATGCCGAAAGAGCCAGTCATTCCAGTCACTCGAGATGAAGCAGTc                                                                                                      <  1:443987/126‑1 (MQ=255)                                              ccTATCAGTTGCTCGAAAGTCAGGGATATATTATCGCACGACCGCAGTCGGGTTATTACGTTGCGCCACAGGCAATAAAAATGCCGAAAGAGCCAGTCATTCCAGTCACTCGAGATGAAGCAGTCGATATCAACACTtat                                                                                       <  1:67610/140‑1 (MQ=255)                                                       tGCTCGAAAGTCAGGGATATATTATCGCACGACCGCAGTCGGGTTATTATGTTGCGCCACAGGCAATAAAAATGCCGAAAGAGCCAGTCATTCCAGTCACTCGAGATGAAGCAGTCGATATCAACACTTATATTTTTGat                                                                              >  2:256921/1‑140 (MQ=255)                                                       tGCTCGAAAGTCAGGGATATATTATCGCACGACCGCAGTCGGGTTATTACGTTGCGCCACAGGCAATAAAAATGCCGAAAGAGCCAGTCATTCCAGTCACTCGAGATGAAGCAGTCGATATCAACACTTATATTTTTGat                                                                              <  1:958190/140‑1 (MQ=255)                                                        gCTCGAAAGTCAGGGATATATTATCGCACGACCGCAGTCGGGTTATTACGTTGCGCCACAGGCAATAAAAATGCCGAAAGAGCCAGTCATTCCAGTCACTCGAGATGAAGCAGTCGATATCAACACTTATATTTTTGata                                                                             >  1:768442/1‑140 (MQ=255)                                                        gCTCGAAAGTCAGGGATATATTATCGCACGACCGCAGTCGGGTTATTACGTTGCGCCACAGGCAATAAAAATGCCGAAAGAGCCAGTCATTCCAGTCACTCGAGATGAAGCAGTCGATATCAACACTTATATTTTTGata                                                                             <  1:627334/140‑1 (MQ=255)                                                         cTCGAAAGTCAGGGATATATTATCGCACGACCGCAGTCGGGTTATTACGTTGCGCCACAGGCAATAAAAATGCCGAAAGAGCCAGTCATTCCAGTCACTCGAGATGAAGCAGTCGATATCAACACTTATATTTTTGatat                                                                            >  1:754059/1‑140 (MQ=255)                                                                     ggATATATTATCGCACGACCGCAGTCGGGTTATTACGTTGCGCCACAGGCAATAAAAATGCCGAAAGAGCCAGTCATTCCAGTCACTCGAGATGAAGCAGTCGATATCAACACTTATATTTTTGATATGTTGCAGGCCAg                                                                <  1:804484/140‑1 (MQ=255)                                                                               tCGCACGACCGCAGTCGGGTTATTACGTTGCGCCACAGGCAATAAAAATGCCGAAAGAGCCAGTCATTCCAGTCACTCGAGATGAAGCAGTCGATATCAACACTTATATTTTTGATATGTTGCAGGCCAGTCGCGATCCg                                                      >  1:532279/1‑140 (MQ=255)                                                                                     gACCGCAGTCGGGTTATTACGTTGCGCCACAGGCAATAAAAATGCCGAAAGAGCCAGTCATTCCAGTCACTCGAGATGAAGCAGTCGATATCAACACTTATATTTTTGATATGTTGCAGGCCAGTCGCGATCCgtcggtc                                                >  2:99601/1‑140 (MQ=255)                                                                                        cGCAGTCGGGTTATTACGTTGCGCCACAGGCAATAAAAATGCCGAAAGAGCCAGTCATTCCAGTCACTCGAGATGAAGCAGTCGATATCAACACTTATATTTTTGATATGTTGCAGGCCAGTCGCGATCCGTCGGTCGtt                                             >  1:664450/1‑140 (MQ=255)                                                                                         gCAGTCGGGTTATTACGTTGCGCCACAGGCAATAAAAATGCCGAAAGAGCCAGTCATTCCAGTCACTCGAGATGAAGCAGTCGATATCAACACTTATATTTTTGATATGTTGCAGGCCAGTCGCGATCCGTCGGTCGTTc                                            >  2:785782/1‑140 (MQ=255)                                                                                                     ttaCGTTGCGCCACAGGCAATAAAAATGCCGAAAGAGCCAGTCATTCCAGTCACTCGAGATGAAGCAGTCGATATCAACACTTATATTTTTGATATGTTGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCgg                                >  1:370830/1‑140 (MQ=255)                                                                                                            gcgcCACAGGCAATAAAAATGCCGAAAGAGCCAGTCATTCCAGTCACTCGAGATGAAGCAGTCGATATCAACACTTATATTTTTGATATGTTGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTTcc                         >  1:792616/1‑140 (MQ=255)                                                                                                             cgcCACAGGCAATAAAAATGCCGAAAGAGCCAGTCATTCCAGTCACTCGAGATGAAGCAGTCGATATCAACACTTATATTTTTGATATGTTGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTTccc                        >  1:50401/1‑140 (MQ=255)                                                                                                             cgcCACAGGCAATAAAAATGCCGAAAGAGCCAGTCATTCCAGTCACTCGAGATGAAGCAGTCGATATCAACACTTATATTTTTGATATGTTGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTTccc                        >  1:34773/1‑140 (MQ=255)                                                                                                               ccACAGGCAATAAAAATGCCGAAAGAGCCAGTCATTCCAGTCACTCGAGATGAAGCAGTCGATATCAACACTTATATTTTTGATATGTTGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTTCCCGa                      >  1:853944/1‑140 (MQ=255)                                                                                                                        aTAAAAATGCCGAAAGAGCCAGTCATTCCAGTCACTCGAGATGAAGCAGTCGATATCAACACTTATATTTTTGATATGTTGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTTCCCGACCCGCGACt             <  2:768442/140‑1 (MQ=255)                                                                                                                           aaaaTGCCGAAAGAGCCAGTCATTCCAGTCACTCGAGATGAAGCAGTCGATATCAACACTTATATTTTTGATATGTTGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTTCCCGACCCGCGACtttt          <  2:664450/140‑1 (MQ=255)                                                                                                                             aaTGCCGAAAGAGCCAGTCATTCCAGTCACTCGAGATGAAGCAGTCGATATCAACACTTATATTTTTGATATGTTGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTTCCCGACCCGCGACTTTTcc        <  2:754059/140‑1 (MQ=255)                                                                                                                               tGCCGAAAGAGCCAGTCATTCCAGTCACTCGAGATGAAGCAGTCGATATCAACACTTATATTTTTGATATGTTGCAGGCCAGTCACGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTTCCCGACCCGCGACTTTTcccc      >  2:375275/1‑140 (MQ=255)                                                                                                                                gCCGAAAGAGCCAGTCATTCCAGTCACTCGAGATGAAGCAGTCGATATCAACACTTATATTTTTGATATGTTGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTTCCCGACCCGCGACTTTTccccc     >  1:211320/1‑140 (MQ=255)                                                                                                                                 ccGAAAGAGCCAGTCATTCCAGTCACTCGAGATGAAGCAGTCGATATCAACACTTATATTTTTGATATGTTGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTTCCCGACCCGCGACTTTTccc       <  2:278876/137‑1 (MQ=255)                                                                                                                                 ccGAAAGAGCCAGTCATTCCAGTCACTCGAGATGAAGCAGTCGATATCAACACTTATATTTTTGATATGTTGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTTCCCGACCCGCGACTTTTccc       >  1:278876/1‑137 (MQ=255)                                                                                                                                   gAAAGAGCCAGTCATTCCAGTCACTCGAGATGAAGCAGTCGATATCAACACTTATATTTTTGATGTGTTGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTTCCCGACCCGCGACTTTTCCCCCTcc  <  2:237374/140‑1 (MQ=255)                                                                                                                                   gAAAGAGCCAGTCATTCCAGTCACTCGAGATGAAGCAGTCGATATCAACACTTATATTTTTGATATGTTGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTTCCCGACCCGCGACTTTTCCCCCTcc  <  2:370830/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       ACCAGGTGGCGCTTTCAGGCATGAGCTTTATGACTGTCAGCCATGCCTATCAGTTGCTCGAAAGTCAGGGATATATTATCGCACGACCGCAGTCGGGTTATTACGTTGCGCCACAGGCAATAAAAATGCCGAAAGCGCCAGTCATTCCAGTCACTCGAGATGAAGCAGTCGATATCAACACTTATATTTTTGATATGTTGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTTCCCGACCCGCGACTTTTCCCCCTCC  >  NC_000913/1510094‑1510363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑