Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,568,5040TG31.7% 230.9 / 26.1 123T6P (ACC→CCC) mdtMmultidrug efflux pump/bile salt:H(+) antiporter/Na(+):H(+) antiporter/K(+):H(+) antiporter
Reads supporting (aligned to +/- strand):  ref base T (18/64);  new base G (38/0);  total (58/65)
Fisher's exact test for biased strand distribution p-value = 7.89e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 123 total aligned reads displayed.

GGGCCAGACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGA‑G‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAATTTCATGTAATC  >  NC_000913/4568369‑4568640
                                                                                                                                       |                                                                                                                                          
gggCCAGACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGAaaaa                                                                                                                                        >  2:1621105/1‑140 (MQ=255)
  gCCAGACTGACATCGGCATTAAACTCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATAAGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTaaaaaa                                                                                                                                      >  1:1493835/1‑140 (MQ=255)
     ggACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTAATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACg                                                                                                                                   <  1:856370/139‑1 (MQ=255)
     aGACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACg                                                                                                                                   <  2:1662355/140‑1 (MQ=255)
       aCTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTg                                                                                                                                 <  1:1337685/140‑1 (MQ=255)
       aCTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGaaaaaaaagag                                                                                                                                 >  2:1054257/1‑136 (MQ=255)
           aCATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCAt                                                                                                                             >  1:286347/1‑140 (MQ=255)
           aCATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAAAGTGGCAt                                                                                                                             >  2:1468738/1‑140 (MQ=255)
            cATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATa                                                                                                                            >  2:945038/1‑140 (MQ=255)
            cATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATa                                                                                                                            <  2:944292/140‑1 (MQ=255)
            cATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATa                                                                                                                            <  2:1345371/140‑1 (MQ=255)
            cATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAAAGTGGCATa                                                                                                                            >  1:566343/1‑140 (MQ=255)
                  cATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATaacaaac                                                                                                                      <  1:1333049/140‑1 (MQ=255)
                   aTTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATaacaaaca                                                                                                                     <  1:656656/140‑1 (MQ=255)
                     tAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGGAAAAAAAGAGGCATAaaaaaaaaa                                                                                                                   >  2:856183/1‑139 (MQ=255)
                     gacagtCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAaacaac                                                                                                                   <  1:617529/135‑1 (MQ=255)
                        aaTCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTcc                                                                                                                <  2:742031/140‑1 (MQ=255)
                        aaTCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTcc                                                                                                                <  2:1348367/140‑1 (MQ=255)
                                aCCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCgg                                                                                                        <  2:1532982/140‑1 (MQ=255)
                                  cacaTTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGga‑g                                                                                                     <  2:1045512/140‑1 (MQ=255)
                                  cacaTTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAAAATGGGAAAAAAAAAAACCCCTTGTCCGga‑g                                                                                                     >  2:773816/1‑140 (MQ=255)
                                    caTTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGA‑G‑aa                                                                                                  <  1:761391/140‑1 (MQ=255)
                                     aTTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGA‑G‑AAc                                                                                                 <  1:426379/140‑1 (MQ=255)
                                     aTTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAAGGGCATAAAAAAAAACTCCTTTTCCggctgtc                                                                                                   >  2:617529/1‑135 (MQ=255)
                                          tGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAATGGCATAAAAAAAAAAACCTTTTCCGGG‑G‑AAACCCaa                                                                                            >  2:457560/1‑140 (MQ=255)
                                            aTCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGA‑G‑AACCCCAACg                                                                                          >  1:1027419/1‑140 (MQ=255)
                                            aTCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGA‑G‑AACCCCAACg                                                                                          >  1:1663296/1‑140 (MQ=255)
                                            aTCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGTGGCATAACAAACAACTCCTTGTCCGGA‑G‑AACCCCAACg                                                                                          >  1:1652118/1‑140 (MQ=255)
                                              cccAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGA‑G‑AACCCCAACggg                                                                                        >  1:678809/1‑140 (MQ=255)
                                              cccAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGA‑G‑AACCCCAACggg                                                                                        >  1:691627/1‑140 (MQ=255)
                                              cccAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAAAGTGGCATAACAAACAACTCCTTGTCCGGA‑G‑AACCCCAACggg                                                                                        >  1:1241098/1‑140 (MQ=255)
                                               ccAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTc                                                                                                           >  1:736558/1‑122 (MQ=255)
                                               ccAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTc                                                                                                           <  2:736558/122‑1 (MQ=255)
                                               ccAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGTGGGCTAACAAAAAAATCCTTTTCCCGA‑G‑AACCCCAACcggg                                                                                       >  2:484585/1‑140 (MQ=255)
                                               ccAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGTGGCATAACAAAAAACTCCTTTTCCGGA‑G‑AACCCCAACgggg                                                                                       >  2:261909/1‑140 (MQ=255)
                                               ccAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGTGGCATAAAAAAAAAC‑ACTTTTCCGGA‑G‑AACCCCCACGGGGa                                                                                      >  2:562901/1‑140 (MQ=255)
                                               ccAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGCGGCATAACAAAAAACTCCTTGTCCGGA‑G‑AACCCCAACgggg                                                                                       >  2:1443403/1‑140 (MQ=255)
                                                cAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGA‑G‑AACCCCAACGGGGa                                                                                      <  2:1652118/140‑1 (MQ=255)
                                                cAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGA‑G‑AACCCCAACGGGGa                                                                                      <  1:945038/140‑1 (MQ=255)
                                                cAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAACGGGGCAAAACAAAAAAATTCTTTTTCGGA‑G‑GACCCCCACggagg                                                                                      >  2:265125/1‑137 (MQ=255)
                                                   gCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGTGGCATAACAAACAACCCCTTGTCCGGA‑G‑AACCCCCACGGGGaaaa                                                                                   >  2:162370/1‑140 (MQ=255)
                                                   gCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGGGGCATAAAAAAAAACTCCTTGTCCGGG‑G‑AACCCCAACGGGGgaaa                                                                                   >  1:17523/1‑140 (MQ=255)
                                                     tGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGA‑G‑AACCCCAACGGGGAAAACg                                                                                 <  1:856183/140‑1 (MQ=255)
                                                     tGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGA‑G‑AACCCCAACGGGGAAAACg                                                                                 <  2:1771379/140‑1 (MQ=255)
                                                     tGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGA‑G‑AACCCCAACGGGGAAAACg                                                                                 <  2:320824/140‑1 (MQ=255)
                                                     tGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGAAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGA‑G‑AACCCCAACGGGGAAAACg                                                                                 <  1:1621105/140‑1 (MQ=255)
                                                     tGGATCAGATCCGTCGACAGATACACAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGA‑G‑AACCCCAACGGGGAAAACg                                                                                 <  2:1759154/140‑1 (MQ=255)
                                                        aTCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGA‑G‑AACCCCAACGGGGAAAACGaaa                                                                              >  1:1528464/1‑140 (MQ=255)
                                                        aTCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGA‑G‑AACCCCAACGGGGAAAACGaaa                                                                              >  1:1030756/1‑140 (MQ=255)
                                                        aTCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAACAGGGGATAAAAAACAACTCCTTGTCCGGA‑G‑AACCCCAACGGGGAAAACaaaa                                                                              >  2:514718/1‑140 (MQ=255)
                                                        aTCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGTGGCATAAAAAAAAAATCCTTGTCCGGA‑G‑AACCCCAACGGGGGAAAAGaaa                                                                              >  2:1490743/1‑140 (MQ=255)
                                                        aTCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAAAGGCATAACAAAAAAAACCTTTTCCGGAGG‑AACCCCACCGGGGaaaaaaaa                                                                               >  2:1375271/1‑136 (MQ=255)
                                                            gATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGA‑G‑AACCCCAACGGGGAAAACGAAATTCa                                                                          <  2:1152415/140‑1 (MQ=255)
                                                             aTCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGTGGGATAAAAAAAAAATCCTTGTCCGGA‑GAAACCCCACCGGGGAAAA‑GAAAATCaa                                                                         >  2:1716674/1‑140 (MQ=255)
                                                             aTCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGTGGCATAACAAACAACTCCTTTTTCCGA‑G‑AACCCCAACCGGGAAAACGAAAATCaa                                                                         >  1:1006519/1‑140 (MQ=255)
                                                             aTCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGTGGCATAAAAAAAAACTCCCTTTCCGGA‑G‑AACCCCCACGGGGGAAACGAAAATCaa                                                                         >  2:1333781/1‑140 (MQ=255)
                                                             aTCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGAGGGCTAAAAAAAAACACCTTGTCCCGA‑G‑AACCCCCACGGGGGAAACGAAAATCaa                                                                         >  2:258556/1‑140 (MQ=255)
                                                              tCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGA‑G‑AACCCCAACGGGGAAAACGAAATTCAAt                                                                        <  1:954760/140‑1 (MQ=255)
                                                                       aGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGGGGCATAAAAAAAAAATCCTTGTCCGGA‑G‑AACCCCCACGGGGGAAAAGAAAATCAATACCGTCggg                                                               >  2:954760/1‑139 (MQ=255)
                                                                        gATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGA‑G‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTa                                                              <  2:1365187/140‑1 (MQ=255)
                                                                        gATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGA‑G‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTa                                                              <  1:1792322/140‑1 (MQ=255)
                                                                        gATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGA‑G‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTa                                                              <  1:1490743/140‑1 (MQ=255)
                                                                         aTACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGAGGCATAAAAAAAAAAACCTTTTCCGGA‑G‑AACCCCAACGGGGAAAAAGAAAATAAAAACCGTCGGGaa                                                             >  2:1347266/1‑140 (MQ=255)
                                                                         aTACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGGGGCATAAAAAACAACTCCTTTTCCGGG‑G‑AACCCCAACGGGGGAAAAGAAAATCAAAACCGTCGGTaa                                                             >  2:1072058/1‑140 (MQ=255)
                                                                            cGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAAAGAGGCCTAAAAAAAAACCCCTTGTCCGGG‑G‑AACCCCAACGGGGGAAACGAAATTAAATACCGGCGGTAATGa                                                          >  2:409300/1‑140 (MQ=255)
                                                                              cagcaAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGA‑G‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACg                                                        <  2:769039/140‑1 (MQ=255)
                                                                              cagcaAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGA‑G‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACg                                                        <  1:773816/140‑1 (MQ=255)
                                                                                 caAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGA‑G‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGaaa                                                     <  2:1528464/140‑1 (MQ=255)
                                                                                 caAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGA‑G‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGaaa                                                     <  1:409300/140‑1 (MQ=255)
                                                                                 caAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGCGGCATAAAAAACAACTCCTTGTCCGGA‑G‑AAACCCAACCGGGGAAACGAAAATTAATACCGTCGGGAATGAAGaaa                                                     >  2:755024/1‑140 (MQ=255)
                                                                                   aaGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGTGGCATAACAAACAACTCCTTGTCCGGG‑G‑AAACCCCACGGGGGAAAAGAAAATCAATACCCTCGGGAATGACGaaaag                                                   >  2:345867/1‑138 (MQ=255)
                                                                                    aGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGAAAAAAAAAGCGGGATAAAAAAAAAATCCTTGTCCCGG‑G‑AACCCCAAAGGGGGAAAAGAAAATCAATAACGCCGGGAATGACGAAAAGc                                                  >  2:1585097/1‑140 (MQ=255)
                                                                                     gTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACT‑CTTGTCCGGA‑G‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAg                                                <  1:562901/140‑1 (MQ=255)
                                                                                      tCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGA‑G‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAg                                                <  1:758021/140‑1 (MQ=255)
                                                                                           cAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGA‑G‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTgag                                           <  1:162370/140‑1 (MQ=255)
                                                                                            aaaaTCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCACAACAAACAACTCCTTGTCCGGA‑G‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTgaga                                          <  2:1493835/140‑1 (MQ=255)
                                                                                                tCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGTGGCATAACAAAAAAATCCTTGTCCGGG‑G‑AACCCCAACGGGGGAAAAGAAAATCAATACCGTCGGGAATGACGAAAAGCAGGGGGGAAggg                                      >  2:157600/1‑140 (MQ=255)
                                                                                                tCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGGGGCATAAAAAACAACCCCTTTTCCGGA‑G‑AACCCCCACGGGGAAAACGAAAATCAATACCGTCGGTAATTACGAAAGGCAGGGGAGAAagg                                      >  2:1188475/1‑140 (MQ=255)
                                                                                                tCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAACGGGCATAACAAAAAACTCCTTGTCCCGA‑G‑AAACCCCACAGGGAAAAAGAAAATCAATAACGTCCGGAATGACGAAAAGGAGGGGAGAAggg                                      >  2:1128489/1‑140 (MQ=255)
                                                                                                      ccATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGA‑G‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACga                                <  1:258556/140‑1 (MQ=255)
                                                                                                      ccATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGA‑G‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACga                                <  1:1347266/140‑1 (MQ=255)
                                                                                                      ccATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGA‑G‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACga                                <  2:691627/140‑1 (MQ=255)
                                                                                                      ccATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGA‑G‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACga                                <  1:157600/140‑1 (MQ=255)
                                                                                                      ccATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGA‑G‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACga                                <  1:1054257/140‑1 (MQ=255)
                                                                                                      ccATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGA‑G‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACga                                <  1:484585/140‑1 (MQ=255)
                                                                                                         tCGGGAAAAACAGGGGGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGA‑G‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGAc                             <  1:514718/140‑1 (MQ=255)
                                                                                                         tCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGA‑G‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGAc                             <  2:1663296/140‑1 (MQ=255)
                                                                                                         tCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGA‑G‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGAc                             <  1:1128489/140‑1 (MQ=255)
                                                                                                         acaggAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGA‑G‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGAc                             <  1:359598/137‑1 (MQ=255)
                                                                                                          cGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGA‑G‑AACCCCCACGGGGAAAAAGAAAATCAATACCCTCGGGAATGACGAAAAGGAGGGGAGGAGGGGAAAGAGAca                            >  2:1394532/1‑139 (MQ=255)
                                                                                                           gggAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGA‑G‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTg                           <  1:1746745/140‑1 (MQ=255)
                                                                                                           gggAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGA‑G‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTg                           <  2:852857/140‑1 (MQ=255)
                                                                                                           gggAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGA‑G‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTg                           <  2:1241098/140‑1 (MQ=255)
                                                                                                              aaaaaCAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGA‑G‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCaa                        <  1:457560/140‑1 (MQ=255)
                                                                                                              aaaaaCAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGA‑G‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCaa                        <  2:1030756/140‑1 (MQ=255)
                                                                                                              aaaaaCAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGA‑G‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCaa                        <  2:1182370/140‑1 (MQ=255)
                                                                                                                     gCGTGGCGGCATGGCGGGGAAAAAAAAGTGGCATAACAAACAACTCCTTTTCCGGA‑G‑AACCCCCACGGGGGAAAAGAAAATCAATACCGTCGGGAATGACGAAAAGGAGGGGGGAAGGGGAACGAGAATGCAAATGTACt                 >  2:895889/1‑140 (MQ=255)
                                                                                                                               aTGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGA‑G‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAATTTCATg       <  1:755024/140‑1 (MQ=255)
                                                                                                                                 ggCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGA‑G‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAATTTCATGTa     <  1:1394532/140‑1 (MQ=255)
                                                                                                                                    gggTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGA‑G‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAATTTCATGTAATc  <  2:721155/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                          
GGGCCAGACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGA‑G‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAATTTCATGTAATC  >  NC_000913/4568369‑4568640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑