Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,894,413	C→A	A178D (GCT→GAT)	yieE →	putative phosphopantetheinyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,894,413	0	C	A	100.0%	307.5 / NA	86	A178D (GCT→GAT)	yieE	putative phosphopantetheinyl transferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (45/41);  total (45/41)

TAAACAAAACGATCCTAACGAAGCGCGGGCGCAGCTCATCACGCTGCGCCGAAGCGTGCTAAAACTAACCGGTGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGCTCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGTCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATAT  >  NC_000913/3894276‑3894548                                                                                                                                          |                                                                                                                                        tAAACAAAACGATCCTAACGAAGCGCGGGCGCAGCTCATCACGCTGCGCCGAAGCGTGCTAAAACTAACCGGTGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATc                                                                                                                                       >  2:342462/1‑140 (MQ=255) tAAACAAAACGATCCTAACGAAGCGCGGGCGCAGCTCATCACGCTGCGCCGAAGCGTGCTAAAACTAACCGGTGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATc                                                                                                                                       >  1:1790240/1‑140 (MQ=255) tAAACAAAACGATCCTAACGAAGCGCGGGCGCAGCTCATCACGCTGCGCCGAAGCGTGCTAAAACTAACCGGTGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATc                                                                                                                                       >  2:734843/1‑140 (MQ=255) tAAACAAAACGATCCTAACGAAGCGCGGGCGCAGCTCATCACGCTGCGCCGAAGCGTGCTAAAACTAACCGGTGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATc                                                                                                                                       >  2:398957/1‑140 (MQ=255)   aaCAAAACGATCCTAACGAAGCGCGGGCGCAGCTCATCACGCTGCGCCGAAGCGTGCTAAAACTAACCGGTGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATCAt                                                                                                                                     <  1:676537/140‑1 (MQ=255)   aaCAAAACGATCCTAACGAAGCGCGGGCGCAGCTCATCACGCTGCGCCGAAGCGTGCTAAAACTAACCGGTGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATCAt                                                                                                                                     <  2:1362066/140‑1 (MQ=255)   aaCAAAACGATCCTAACGAAGCGCGGGCGCAGCTCATCACGCTGCGCCGAAGCGTGCTAAAACTAACCGGTGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATCAt                                                                                                                                     <  1:1615954/140‑1 (MQ=255)   aaCAAAACGATCCTAACGAAGCGCGGGCGCAGCTCATCACGCTGCGCCGAAGCGTGCTAAAACTAACCGGTGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATCAt                                                                                                                                     >  2:1153259/1‑140 (MQ=255)     cAAAACGATCCTAACGAAGCGCGGGCGCAGCTCATCACGCTGCGCCGAAGCGTGCTAAAACTAACCGGTGATGTTTTGAATGACGATCCGCGCGGTCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATCATGt                                                                                                                                   >  1:1245962/1‑140 (MQ=255)      aaaaCGATCCTAACGAAGCGCGGGCGCAGCTCATCACGCTGCGCCGAAGCGTGCTAAAACTAACCGGTGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATCATGTa                                                                                                                                  <  1:143944/140‑1 (MQ=255)        aaCGATCCTAACGAAGCGCGGGCGCAGCTCATCACGCTGCGCCGAAGCGTGCTAAAACTAACCGGTGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATCATGt                                                                                                                                   <  2:142186/137‑1 (MQ=255)        aaCGATCCTAACGAAGCGCGGGCGCAGCTCATCACGCTGCGCCGAAGCGTGCTAAAACTAACCGGTGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATCATGt                                                                                                                                   >  1:142186/1‑137 (MQ=255)        aaCGATCCTAACGAAGCGCGGGCGCAGCTCATCACGCTGCGCCGAAGCGTGCTAAAACTAACCGGTGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATCATGTaaa                                                                                                                                <  2:815330/140‑1 (MQ=255)        aaCGATCCTAACGAAGCGCGGGCGCAGCTCATCACGCTGCGCCGAAGCGTGCTAAAACTAACCGGTGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATCATGTaaa                                                                                                                                <  2:494005/140‑1 (MQ=255)        aaCGATCCTAACGAAGCGCGGGCGCAGCTCATCACGCTGCGCCGAAGCGTGCTAAAACTAACCGGTGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATCATGTaaa                                                                                                                                <  2:537327/140‑1 (MQ=255)        aaCGATCCTAACGAAGCGCGGGCGCAGCTCATCACGCTGCGCCGAAGCGTGCTAAAACTAACCGGTGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATCATGTaaa                                                                                                                                <  1:363172/140‑1 (MQ=255)        aaCGATCCTAACGAAGCGCGGGCGCAACTCATCACGCTGCGCCGAAGCGTGCTAAAACTAACCGGTGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATCATGTaaa                                                                                                                                >  2:892515/1‑140 (MQ=255)           gATCCTAACGAAGCGCGGGCGCAGCTCATCACGCTGCGCCGAAGCGTGCTAAAACTAACCGGTGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATCATGTAAATCa                                                                                                                             >  1:581230/1‑140 (MQ=255)           gATCCTAACGAAGCGCGGGCGCAGCTCATCACGCTGCGCCGAAGCGTGCTAAAACTAACCGGTGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATCATGTAAATCa                                                                                                                             >  1:873171/1‑140 (MQ=255)             tCCTAACGAAGCGCGGGCGCAGCTCATCACGCTGCGCCGAAGCGTGCTAAAACTAACCGGTGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATCATGTAAATCATg                                                                                                                           >  2:59715/1‑140 (MQ=255)               cTAACGAAGCGCGGGCGCAGCTCATCACGCTGCGCCGAAGCGTGCTAAAACTAACCGGTGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATCATGTAAATCATGTa                                                                                                                         >  1:1314800/1‑140 (MQ=255)                tAACGAAGCGCGGGCGCAGCTCATCACGCTGCGCCGAAGCGTGCTAAAACTAACCGGTGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATCATGTAAATCATGTAg                                                                                                                        <  2:581230/140‑1 (MQ=255)                tAACGAAGCGCGGGCGCAGCTCATCACGCTGCGCCGAAGCGTGCTAAAACTAACCGGTGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATCATGTAAATCATGTAg                                                                                                                        <  1:447116/140‑1 (MQ=255)                  aCGAAGCGCGGGCGCAGCTCATCACGCTGCGCCGAAGCGTGCTAAAACTAACCGGTGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATCATGTAAATCATGTAGaa                                                                                                                      <  1:184543/140‑1 (MQ=255)                  aCGAAGCGCGGGCGCAGCTCATCACGCTGCGCCGAAGCGTGCTAAAACTAACCGGTGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATCATGTAAATCATGTAGaa                                                                                                                      >  2:400469/1‑140 (MQ=255)                  aCGAAGCGCGGGCGCAGCTCATCACGCTGCGCCGAAGCGTGCTAAAACTAACCGGTGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATCATGTAAATCATGTAGaa                                                                                                                      <  2:54585/140‑1 (MQ=255)                  aCGAAGCGCGGGCGCAGCTCATCACGCTGCGCCGAAGCGTGCTAAAACTAACCGGTGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATCATGTAAATCATGTAGaa                                                                                                                      <  2:1790240/140‑1 (MQ=255)                      aGCGCGGGCGCAGCTCATCACGCTGCGCCGAAGCGTGCTAAAACTAACCGGTGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATCATGTAAATCATGTAGAAGCGt                                                                                                                  >  2:131914/1‑140 (MQ=255)                        cgcgGGCGCAGCTCATCACGCTGCGCCGAAGCGTGCTAAAACTAACCGGTGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATCATGTaaa                                                                                                                                >  1:1027526/1‑124 (MQ=255)                        cgcgGGCGCAGCTCATCACGCTGCGCCGAAGCGTGCTAAAACTAACCGGTGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATCATGTaaa                                                                                                                                <  2:1027526/124‑1 (MQ=255)                        cgcgGGCGCAGCTCATCACGCTGCGCCGAAGCGTGCTAAAACTAACCGGTGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATCATGTAAATCATGTAGAAGCGTTa                                                                                                                >  1:597324/1‑140 (MQ=255)                        cgcgGGCGCAGCTCATCACGCTGCGCCGAAGCGTGCTAAAACTAACCGGTGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATCATGTAAATCATGTAGAAGCGTTa                                                                                                                >  2:808728/1‑140 (MQ=255)                          cgGGCGCAGCTCATCACGCTGCGCCGAAGCGTGCTAAAACTAACCGGTGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATCATGTAAATCATGTAGAAGCGTTATg                                                                                                              <  1:1153259/140‑1 (MQ=255)                            ggCGCAGCTCATCACGCTGCGCCGAAGCGTGCTAAAACTAACCGGTGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATCATGTAAATCATGTAGAAGCGTTATGCg                                                                                                            >  2:424432/1‑140 (MQ=255)                            ggCGCAGCTCATCACGCTGCGCCGAAGCGTGCTAAAACTAACCGGTGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATCATGTAAATCATGTAGAAGCGTTATGCg                                                                                                            <  2:549567/140‑1 (MQ=255)                                  gCTCATCACGCCGCGCCGAAGCGTGCTAAAACTAACCGGTGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATCATGTAAATCATGTAGAAGCGTTATGCGACGCgg                                                                                                      >  2:761510/1‑140 (MQ=255)                                   cTCATCACGCTGCGCCGAAGCGTGCTAAAACTAACCGGTGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATCATGTAAATCATGTAGAAGCGTTATGCGACGCGGa                                                                                                     <  1:400469/140‑1 (MQ=255)                                     catcaCGCTGCGCCGAAGCGTGCTAAAACTAACCGGTGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAg                                                                                                   <  1:808728/140‑1 (MQ=255)                                     catcaCGCTGCGCCGAAGCGTGCTAAAACTAACCGGTGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAg                                                                                                   <  1:424432/140‑1 (MQ=255)                                      atcaCGCTGCGCCGAAGCGTGCTAAAACTAACCGGTGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGa                                                                                                  >  1:84654/1‑140 (MQ=255)                                        caCGCTGCGCCGAAGCGTGCTAAAACTAACCGGTGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACg                                                                                                <  1:398957/140‑1 (MQ=255)                                                       cGTGCTAAAACTAACCGGTGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCg                                                                                 <  1:761510/140‑1 (MQ=255)                                                         tGCTAAAACTAACCGGTGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTcctgt                                                                               >  1:1465205/1‑137 (MQ=255)                                                              aaaCTAACCGGTGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGt                                                                          <  1:342462/140‑1 (MQ=255)                                                              aaaCTAACCGGTGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGt                                                                          <  1:734843/140‑1 (MQ=255)                                                                 cTAACCGGTGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCAc                                                                       >  1:1345961/1‑140 (MQ=255)                                                                    aCCGGTGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGcc                                                                    >  2:666301/1‑140 (MQ=255)                                                                    aCCGGTGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGcc                                                                    >  2:426637/1‑140 (MQ=255)                                                                       ggTGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCAc                                                                 <  1:666301/140‑1 (MQ=255)                                                                       ggTGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCAc                                                                 <  2:1314800/140‑1 (MQ=255)                                                                       ggTGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCAc                                                                 <  1:892515/140‑1 (MQ=255)                                                                        gTGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACg                                                                >  1:930510/1‑140 (MQ=255)                                                                         tGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTgg                                                                              >  2:1440318/1‑125 (MQ=255)                                                                         tGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTgg                                                                              <  1:1440318/125‑1 (MQ=255)                                                                           aTGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCg                                                                            <  2:1754815/125‑1 (MQ=255)                                                                           aTGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCg                                                                            >  1:1754815/1‑125 (MQ=255)                                                                                 tGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAg                                                       <  2:1345961/140‑1 (MQ=255)                                                                                  gAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGc                                                      >  1:1574811/1‑140 (MQ=255)                                                                                   aaTGACGATCCGCGCGGTCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCt                                                     <  2:1245962/140‑1 (MQ=255)                                                                                   aaTGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCt                                                     >  2:1631589/1‑140 (MQ=255)                                                                                   aaTGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATCGAAAAGCt                                                     >  2:1311221/1‑140 (MQ=255)                                                                                     tGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCa                                                   <  1:426637/140‑1 (MQ=255)                                                                                       aCGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAgt                                                 >  1:1129397/1‑140 (MQ=255)                                                                                        cGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCa                                                   <  2:970765/137‑1 (MQ=255)                                                                                        cGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCa                                                   >  1:970765/1‑137 (MQ=255)                                                                                            ccGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGATCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGTCTg                                            <  1:131914/140‑1 (MQ=255)                                                                                                              ctgcCCATTGCCGGACGCCTGAAATGTGATCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGTCTGGGAGTTAGATGGCAGaca                          >  2:252222/1‑140 (MQ=255)                                                                                                              ctgcCCATTGCCGGACGCCTGAAATGTGATCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGTCTGGGAGTTAGATGGCAAaca                          >  1:114007/1‑140 (MQ=255)                                                                                                              ctgcCCATTGCCGGACGCCTGAAATGTGATCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGTCTGGGAGTTAGATGGCAAaca                          >  2:219926/1‑140 (MQ=255)                                                                                                              ctgcCCATTGCCGGACGCCTGAAATGTGATCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGTCTGGGAGTTAGATGGCAAaca                          >  1:1268515/1‑140 (MQ=255)                                                                                                              ctgcCCATTGCCGGACGCCTGAAATGTGATCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGTCTGGGAGTTAGATGGCAAaca                          >  1:366246/1‑140 (MQ=255)                                                                                                              ctgcCCATTGCCGGACGCCTGAAATGTGATCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGTCTGGGAGTTAGATGGCAAaca                          >  1:1386405/1‑140 (MQ=255)                                                                                                              ctgcCCATTGCCGGACGCCTGAAATGTGATCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGTCTGGGAGTTAGATGGCAAaca                          >  2:736160/1‑140 (MQ=255)                                                                                                              ctgcCCATTGCCGGACGCCTGAAATGTGATCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGTCTCGGGGTTAGATGGCAAaca                          >  2:932796/1‑140 (MQ=255)                                                                                                                gcCCATTGCCGGACGCCTGAAATGTGATCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGTCTGGGAGTTAGATGGCAAACACg                        >  2:963609/1‑140 (MQ=255)                                                                                                                gcCCATTGCCGGACGCCTGAAATGTGATCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGTCTGGGAGTTAGATGGCAAACACg                        >  2:1456077/1‑140 (MQ=255)                                                                                                                 cccATTGCCGGACGCCTGAAATGTGATCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGTCTGGGAGTTAGATGGCAAACACgg                       <  2:114007/140‑1 (MQ=255)                                                                                                                 cccATTGCCGGACGCCTGAAATGTGATCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGTCTGGGAGTTAGATGGCAAACACgg                       <  2:366246/140‑1 (MQ=255)                                                                                                                           gACGCCTGAAATGTGATCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGTCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGc             <  2:597324/140‑1 (MQ=255)                                                                                                                             cGCCTGAAATGTGATCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGTCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCt           <  2:1574811/140‑1 (MQ=255)                                                                                                                                cTGAAATGTGATCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGTCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGcc        >  1:1520147/1‑140 (MQ=255)                                                                                                                                cTGAAATGTGATCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGATGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGTCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGcc        >  1:1378227/1‑140 (MQ=255)                                                                                                                                  gAAATGTGATCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGTCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCgg      <  1:59715/140‑1 (MQ=255)                                                                                                                                  gAAATGTGATCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGTCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCgg      <  2:1129397/140‑1 (MQ=255)                                                                                                                                  gAAATGTGATCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGTCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCgg      <  2:930510/140‑1 (MQ=255)                                                                                                                                     aTGTGATCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGTCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGata   >  2:1287345/1‑140 (MQ=255)                                                                                                                                      tgtgATCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGTCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGatat  >  2:259504/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        TAAACAAAACGATCCTAACGAAGCGCGGGCGCAGCTCATCACGCTGCGCCGAAGCGTGCTAAAACTAACCGGTGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGCTCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGTCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATAT  >  NC_000913/3894276‑3894548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑