BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,371,694	G→T	D224Y (GAC→TAC)	groL →	chaperonin GroEL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,371,694	0	G	T	99.2%	474.3 / ‑3.4	133	D224Y (GAC→TAC)	groL	chaperonin GroEL
Reads supporting (aligned to +/- strand): ref base G (0/1); new base T (68/64); total (68/65)
Fisher's exact test for biased strand distribution p-value = 4.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 136 total aligned reads displayed.

GGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCAGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTGACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCGCTGCTGATCATCGCTGAAGATGTAGAAGGCGAAGCGCTGGCAACTCTGGTTGTTAACACCATGCGT  >  NC_000913/4371562‑4371828

ggTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCAGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGaa                                                                                                                                 >  1:653336/1‑140 (MQ=255)

  tACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCAGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGaaaa                                                                                                                               <  1:1333133/140‑1 (MQ=255)

            cAGGACGAACTGGACGTGGTTGAAGGTATGCAGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACAt                                                                                                                     >  1:1561953/1‑140 (MQ=255)

            cAGGACGAACTGGACGTGGTTGAAGGTATGCAGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACAt                                                                                                                     >  2:343468/1‑140 (MQ=255)

                    aCTGGACGTGGTTGAAGGTATGCAGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGaaa                                                                                                             >  2:1360171/1‑140 (MQ=255)

                    aCTGGACGTGGTTGAAGGTATGCAGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGaaa                                                                                                             <  1:1661637/140‑1 (MQ=255)

                    aCTGGACGTGGTTGAAGGTATGCAGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGaaa                                                                                                             <  1:932758/140‑1 (MQ=255)

                    aCTGGACGTGGTTGAAGGTATGCAGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGaaa                                                                                                             >  2:1616216/1‑140 (MQ=255)

                      tGGACGTGGTTGAAGGTATGCAGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAAtg                                                                                                           >  2:1485672/1‑140 (MQ=255)

                        gACGTGGTTGAAGGTATGCAGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAAtgct                                                                                                         <  2:1186922/140‑1 (MQ=255)

                          cGTGGTTGAAGGTATGCAGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAAtgctgc                                                                                                       <  1:1360171/140‑1 (MQ=255)

                          cGTGGTTGAAGGTATGCAGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAAtgctgc                                                                                                       <  1:1616216/140‑1 (MQ=255)

                          cGTGGTTGAAGGTATGCAGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAAtgctgc                                                                                                       >  1:1104081/1‑140 (MQ=255)

                          cGTGGTTGAAGGTATGCAGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAAtgctgc                                                                                                       >  1:993681/1‑140 (MQ=255)

                            tGGTTGAAGGTATGCAGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCg                                                                                                     <  2:1104081/140‑1 (MQ=255)

                             ggTTGAAGGTATGCAGTTCGACCGTGACTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCgg                                                                                                    <  1:155348/140‑1 (MQ=255)

                              gTTGAAGGTATGCAGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGt                                                                                                   >  2:266983/1‑140 (MQ=255)

                                  aaGGTATGCAGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAAtgctgc                                                                                                       <  1:651720/132‑1 (MQ=255)

                                  aaGGTATGCAGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAAtgctgc                                                                                                       >  2:651720/1‑132 (MQ=255)

                                    ggTATGCAGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGa                                                                                             >  1:572010/1‑140 (MQ=255)

                                      tATGCAGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTGACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAg                                                                                           <  1:629822/140‑1 (MQ=255)

                                       aTGCAGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGc                                                                                          <  1:747169/140‑1 (MQ=255)

                                       aTGCAGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGc                                                                                          >  1:1806552/1‑140 (MQ=255)

                                        tGCAGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCt                                                                                         <  2:1102626/140‑1 (MQ=255)

                                        tGCAGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCt                                                                                         >  1:1395573/1‑140 (MQ=255)

                                             ttCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCt                                                                                         <  2:1011805/135‑1 (MQ=255)

                                             ttCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCt                                                                                         >  1:1011805/1‑135 (MQ=255)

                                                gACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCaa                                                                                 <  2:1252466/140‑1 (MQ=255)

                                                  ccGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAg                                                                               <  2:401177/140‑1 (MQ=255)

                                                      ggCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGACTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAgg                                                                           >  2:1124085/1‑140 (MQ=255)

                                                       gCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGc                                                                          >  1:514585/1‑140 (MQ=255)

                                                           ccTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAAc                                                                      <  2:1806552/140‑1 (MQ=255)

                                                            cTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAAc                                                                      >  2:293990/1‑139 (MQ=255)

                                                              gTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAAc                                                                      >  2:578020/1‑137 (MQ=255)

                                                              gTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAAc                                                                      <  1:578020/137‑1 (MQ=255)

                                                              gTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCgc                                                                   >  1:1569910/1‑140 (MQ=255)

                                                               tctcCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCgct                                                                  >  1:1804624/1‑140 (MQ=255)

                                                                ctcCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCgctg                                                                 >  2:236373/1‑140 (MQ=255)

                                                                 tcCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCgctgc                                                                >  2:1588041/1‑140 (MQ=255)

                                                                     tACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCGCTGCTGat                                                            <  2:1435722/140‑1 (MQ=255)

                                                                     tACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCGCTGCTGat                                                            >  2:1598369/1‑140 (MQ=255)

                                                                     tACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCGCTGCTGat                                                            <  1:266983/140‑1 (MQ=255)

                                                                      aCTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCGCTGCTGatc                                                           >  1:356749/1‑140 (MQ=255)

                                                                         tcatcaACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCg                                                                    <  1:1646954/128‑1 (MQ=255)

                                                                         tcatcaACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCGCTGCTGatcatc                                                        >  1:78490/1‑140 (MQ=255)

                                                                         tcatcaACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCGCTGCTGatcatc                                                        >  2:608126/1‑140 (MQ=255)

                                                                         tcatcaACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCGCTGCTGatcatc                                                        >  2:1613377/1‑140 (MQ=255)

                                                                         tcatcaACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCGCTGCTGatcatc                                                        >  2:466383/1‑140 (MQ=255)

                                                                         tcatcaACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCGCTGCTGatcatc                                                        >  1:729239/1‑140 (MQ=255)

                                                                           atcaACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAAc                                                                      >  2:216550/1‑124 (MQ=255)

                                                                           atcaACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCGCTGCTGatc                                                           <  1:1677111/135‑1 (MQ=255)

                                                                           atcaACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCGCTGCTGatc                                                           >  2:1677111/1‑135 (MQ=255)

                                                                           atcaACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCGCTGCTGATCATCGc                                                      <  2:1062099/140‑1 (MQ=255)

                                                                            tcaACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAAc                                                                      <  2:981347/123‑1 (MQ=255)

                                                                            tcaACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAAc                                                                      >  1:981347/1‑123 (MQ=255)

                                                                            tcaACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCGCTGCTGATCATCGCt                                                     <  2:514585/140‑1 (MQ=255)

                                                                            tcaACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCGCTGCTGATCATCGCt                                                     <  1:145732/140‑1 (MQ=255)

                                                                            tcaACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCGCTGCTGATCATCGCt                                                     <  1:767285/140‑1 (MQ=255)

                                                                            tcaACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCGCTGCTGATCATCGCt                                                     <  1:236373/140‑1 (MQ=255)

                                                                            tcaACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCGCTGCTGATCATCGCt                                                     >  2:1505459/1‑140 (MQ=255)

                                                                             caacaaGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCGCTGCTGATCATCGCTg                                                    <  1:105195/140‑1 (MQ=255)

                                                                             caacaaGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCGCTGCTGATCATCGCTg                                                    <  2:1804624/140‑1 (MQ=255)

                                                                                caaGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCGCTGCTGATCATCGCTGAAg                                                 <  2:750029/140‑1 (MQ=255)

                                                                                  aGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCGCTGCTGATCATCGCTGAAGAt                                               >  1:150478/1‑140 (MQ=255)

                                                                                  aGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCGCTGCTGATCATCGCTGAAGAt                                               >  2:818520/1‑140 (MQ=255)

                                                                                     cGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCGCTGCTGATCATCGCTGAAGATGTa                                            >  1:290979/1‑140 (MQ=255)

                                                                                      ggAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCGCTGCTGATCATCGCTGAAGATGTAg                                           <  1:1485672/140‑1 (MQ=255)

                                                                                              gcgcAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCGCTGCTGATCATCGCTGAAGATGTAGAAGGCGaa                                   <  2:1391707/140‑1 (MQ=255)

                                                                                              gcgcAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCGCTGCTGATCATCGCTGAAGATGTAGAAGGCGaa                                   >  1:340565/1‑140 (MQ=255)

                                                                                              gcgcAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCGCTGCTGATCATCGCTGAAGATGTAGAAGGCGaa                                   >  2:561255/1‑140 (MQ=255)

                                                                                              gcgcAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCGCTGCTGATCATCGCTGAAGATGTAGAAGGCGaa                                   >  1:854757/1‑140 (MQ=255)

                                                                                                 cAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCGCTGCTGATCATCGCTGAAGATGTAGAAGGCGAAgcg                                <  1:1118714/140‑1 (MQ=255)

                                                                                                   gTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCGCTGCTGATCATCGCTGAAGATGTAGAAGGCGAAGCGCt                              >  2:669013/1‑140 (MQ=255)

                                                                                                   gTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCGCTGCTGATCATCGCTGAAGATGTAGAAGGCGAAGCGCt                              <  1:1589290/140‑1 (MQ=255)

                                                                                                      gAACTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCGCTGCTGATCATCGCTGAAGATGTAGAAGGCGAAGCGCTGGc                           <  2:949351/140‑1 (MQ=255)

                                                                                                        aCTGGAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCGCTGCTGATCATCGCTGAAGATGTAGAAGGCGAAGCGCTGGCaa                         >  1:651128/1‑140 (MQ=255)

                                                                                                           ggAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCGCTGCTGATCATCGCTGAAGATGTAGAAGGCGAAGCGCTGGCAActc                      <  2:572010/140‑1 (MQ=255)

                                                                                                           ggAAAGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCGCTGCTGATCATCGCTGAAGATGTAGAAGGCGAAGCGCTGGCAActc                      <  2:150478/140‑1 (MQ=255)

                                                                                                             aaaGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCGCTGCTGATCATCGCTGAAGATGTAGAAGGCGAAGCGCTGGCAACTCTg                    <  1:561255/140‑1 (MQ=255)

                                                                                                             aaaGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCGCTGCTGATCATCGCTGAAGATGTAGAAGGCGAAGCGCTGGCAACTCTg                    <  1:1588041/140‑1 (MQ=255)

                                                                                                             aaaGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCGCTGCTGATCATCGCTGAAGATGTAGAAGGCGAAGCGCTGGCAACTCTg                    <  1:1598369/140‑1 (MQ=255)

                                                                                                             aaaGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCGCTGCTGATCATCGCTGAAGATGTAGAAGGCGAAGCGCTGGCAACTCTg                    <  1:1054479/140‑1 (MQ=255)

                                                                                                             aaaGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCGCTGCTGATCATCGCTGAAGATGTAGAAGGCGAAGCGCTGGCAACTCTg                    <  1:392792/140‑1 (MQ=255)

                                                                                                              aaGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCGCTGCTGATCATCGCTGAAGATGTAGAAGGCGAAGCGCTGGCAActc                      >  2:1811211/1‑137 (MQ=255)

                                                                                                              aaGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCGCTGCTGATCATCGCTGAAGATGTAGAAGGCGAAGCGCTGGCAActc                      <  1:1811211/137‑1 (MQ=255)

                                                                                                              aaGCCCGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCGCTGCTGATCATCGCTGAAGATGTAGAAGGCGAAGCGCTGGCAACTCTgg                   <  2:356749/140‑1 (MQ=255)

                                                                                                                 cccGTTCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCGCTGCTGATCATCGCTGAAGATGTAGAAGGCGAAGCGCTGGCAACTCTGgttg                >  1:816415/1‑140 (MQ=255)

                                                                                                                     ttCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCGCTGCTGATCATCGCTGAAGATGTAGAAGGCGAAGCGCTGGCAACTCTGGTTGTTaa            >  1:1214180/1‑140 (MQ=255)

                                                                                                                      tCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCGCTGCTGATCATCGCTGAAGATGTAGAAGGCGAAGCGCTGGCAACTCTg                    <  2:1793380/131‑1 (MQ=255)

                                                                                                                      tCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCGCTGCTGATCATCGCTGAAGATGTAGAAGGCGAAGCGCTGGCAACTCTg                    >  1:1793380/1‑131 (MQ=255)

                                                                                                                      tCATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCGCTGCTGATCATCGCTGAAGATGTAGAAGGCGAAGCGCTGGCAACTCTGGTTGTTAac           >  1:673761/1‑140 (MQ=255)

                                                                                                                       cATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCGCTGCTGATCATCGCTGAAGATGTAGAAGGCGAAGCGCTGGCAACTCTGGTTGTTAaca          <  2:1569910/140‑1 (MQ=255)

                                                                                                                       cATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCGCTGCTGATCATCGCTGAAGATGTAGAAGGCGAAGCGCTGGCAACTCTGGTTGTTAaca          <  2:709457/140‑1 (MQ=255)

                                                                                                                       cATCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCGCTGCTGATCATCGCTGAAGATGTAGAAGGCGAAGCGCTGGCAACTCTGGTTGTTAaca          <  2:841927/140‑1 (MQ=255)

                                                                                                                         tCCTGCTGGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCGCTGCTGATCATCGCTGAAGATGTAGAAGGCGAAGCGCTGGCAACTCTGGTTGTTAACAcc        <  2:729239/140‑1 (MQ=255)

                                                                                                                           ctgctgGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCGCTGCTGATCATCGCTGAAGATGTAGAAGGCGAAGCGCTGGCAACTCTg                    >  1:1403304/1‑126 (MQ=255)

                                                                                                                           ctgctgGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCGCTGCTGATCATCGCTGAAGATGTAGAAGGCGAAGCGCTGGCAACTCTg                    <  2:1403304/126‑1 (MQ=255)

                                                                                                                           ctgctgGCTTACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCGCTGCTGATCATCGCTGAAGATGTAGAAGGCGAAGCGCTGGCAACTCTGGTTGTTAACACCAt      >  2:1185688/1‑140 (MQ=255)

                                                                                                                              ctggcttACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCGCTGCTGATCATCGCTGAAGATGTAGAAGGCGAAGCGCTGGCAACTCTGGTTGTTAACACCATGCg   >  2:1564258/1‑140 (MQ=255)

                                                                                                                               tggcttACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCGCTGCTGATCATCGCTGAAGATGTAGAAGGCGAAGCGCTGGCAACTCTGGTTGTTAACACCATGCGt  <  1:1185688/140‑1 (MQ=255)

GGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCAGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTGACAAGAAAATCTCCAACATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCGCTGCTGATCATCGCTGAAGATGTAGAAGGCGAAGCGCTGGCAACTCTGGTTGTTAACACCATGCGT  >  NC_000913/4371562‑4371828

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`