Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,219,272	C→A	A70A (GCG→GCT)	osmF ←	glycine betaine ABC transporter periplasmic binding protein OsmF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,219,272	0	C	A	100.0%	233.2 / NA	66	A70A (GCG→GCT)	osmF	glycine betaine ABC transporter periplasmic binding protein OsmF
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (32/34);  total (32/34)

TGTGCTCCGAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAGCGCCATTGCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAGTAATCGCCCCGCGCACCACAGGAGTCGTTCCGAGTTGCACTTTATTCACCGTCGGTACGCCGTGGCTTTCCAGCACCTGCAAAATGATATTGCCGAGTAGCGCACCTTCGGTATCGATTTTTGAACCGACTTTAACGG  >  NC_000913/2219136‑2219405                                                                                                                                         |                                                                                                                                      tgtgCTCCGAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAGCGCCATTGCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAGTAATAGcc                                                                                                                                    <  2:317253/140‑1 (MQ=255) tgtgCTCCGAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAGCGCCATTGCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAGTAATAGcc                                                                                                                                    >  2:216450/1‑140 (MQ=255)      tCCGAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAGCGCCATTGCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAGTAATAGCCCcgcg                                                                                                                               <  1:57345/140‑1 (MQ=255)          aaTCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAGCGCCATTGCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAGTAATAGCCCCGCGcacc                                                                                                                           >  2:984832/1‑140 (MQ=255)          aaTCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAGCGCCATTGCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAGTAATAGCCCCGCGcacc                                                                                                                           >  1:787021/1‑140 (MQ=255)            tCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAGCGCCATTGCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAGTAATAGCCCCGCGcaccac                                                                                                                         <  2:1491764/140‑1 (MQ=255)                 cTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAGCGCCATTGCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAGTAATAGCCCCGCGCACCACAGGAg                                                                                                                    <  2:1010273/140‑1 (MQ=255)                      tGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAGCGCCATTGCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAGTAATAGCCCCGCGCACCACAGGAGTCGtt                                                                                                               <  2:1678060/140‑1 (MQ=255)                             ctcGTAACCTTGCTGCGCGTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAGCGCCATTGCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAGTAATAGCCCCGCGCACCACAGGAGTCGTTCCGAGtt                                                                                                        <  1:1014906/140‑1 (MQ=255)                             ctcGTAACCTTGCTGCGCGTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAGCGCCATTGCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAGTAATAGCCCCGCGCACCACAGGAGTCGTTCCGAGtt                                                                                                        <  1:386128/140‑1 (MQ=255)                              tcGTAACCTTGCTGCGCGTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAGCGCCATTGCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAGTAATAGCCCCGCGCACCACAGGAGTCGTTCCGAGTTg                                                                                                       <  2:1346300/140‑1 (MQ=255)                              tcGTAACCTTGCTGCGCGTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAGCGCCATTGCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAGTAATAGCCCCGCGCACCACAGGAGTCGTTCCGAGTTg                                                                                                       <  1:765512/140‑1 (MQ=255)                                       tgctgcGCGTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAGCGCCATTGCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAGTAATAGCCCCGCGCACCACAGGAGTCGTTCCGAGTTGCACTTTAtt                                                                                              >  1:1741575/1‑140 (MQ=255)                                       tgctgcGCGTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAGCGCCATTGCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAGTAATAGCCCCGCGCACCACAGGAGTCGTTCCGAGTTGCACTTTAtt                                                                                              >  2:742312/1‑140 (MQ=255)                                       tgctgcGCGTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAGCGCCATTGCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAGTAATAGCCCCGCGCACCACAGGAGTCGTTCCGAGTTGCACTTTAtt                                                                                              >  1:1347804/1‑140 (MQ=255)                                        gctgcGCGTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAGCGCCATTGCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAGTAATAGCCCCGCGCACCACAGGAGTCGTTCCGAGTTGCACTTTATTc                                                                                             >  2:1417801/1‑140 (MQ=255)                                        gctgcGCGTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAGCGCCATTGCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAGTAATAGCCCCGCGCACCACAGGAGTCGTTCCGAGTTGCACTTTATTc                                                                                             >  2:1555542/1‑140 (MQ=255)                                         ctgcGCGTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAGCGCCATTGCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAGTAATAGCCCCGCGCACCACAGGAGTCGTTCCGAGTTGCACTTTATTCa                                                                                            >  2:1247216/1‑140 (MQ=255)                                          tgcGCGTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAGCGCCATTGCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAGTAATAGCCCCGCGCACCACAGGAGTCGTTCCGAGTTGCACTTTATTCAc                                                                                           <  2:787021/140‑1 (MQ=255)                                              cgTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAGCGCCATTGCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAGTAATAGCCCCGCGCACCACAGGAGTCGTTCCGAGtt                                                                                                        >  2:1152136/1‑123 (MQ=255)                                              cgTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAGCGCCATTGCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAGTAATAGCCCCGCGCACCACAGGAGTCGTTCCGAGtt                                                                                                        <  1:1152136/123‑1 (MQ=255)                                              cgTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAGCGCCATTGCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAGTAATAGCCCCGCGCACCACAGGAGTCGTTCCGAGTTGCACTTTATTCACCGTc                                                                                       >  2:21369/1‑140 (MQ=255)                                                  tttCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAGCGCCATTGCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAGTAATAGCCCCGCGCACCACAGGAGTCGTTCCGAGTTGCACTTTATTCACCGTCGGTa                                                                                   >  2:1458720/1‑140 (MQ=255)                                                       aCGCTGCATCGTTTTCATCTTTAAAGAAGAAAGCGCCATTGCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAGTAATAGCCCCGCGCACCACAGGAGTCGTTCCGAGTTGCACTTTATTCACCGTCGGTACGcca                                                                              <  1:1555542/140‑2 (MQ=255)                                                           tGCATCGTTTTCATCTTTAAAGAAGAAAGCGCCATTGCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAGTAATAGCCCCGCGCACCACAGGAGTCGTTCCGAGTTGCACTTTATTCACCGTCGGTACGCCGTGGc                                                                          <  1:1544023/140‑1 (MQ=255)                                                             cATCGTTTTCATCTTTAAAGAAGAAAGCGCCATTGCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAGTAATAGCCCCGCGCACCACAGGAGTCGTTCCGAGTTGCACTTTATTCACCGTCGGTACGCCGt                                                                             >  1:544051/1‑135 (MQ=255)                                                             cATCGTTTTCATCTTTAAAGAAGAAAGCGCCATTGCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAGTAATAGCCCCGCGCACCACAGGAGTCGTTCCGAGTTGCACTTTATTCACCGTCGGTACGCCGt                                                                             <  2:544051/135‑1 (MQ=255)                                                             cATCGTTTTCATCTTTAAAGAAGAAAGCGCCATTGCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAGTAATAGCCCCGCGCACCACAGGAGTCGTTCCGAGTTGCACTTTATTCACCGTCGGTACGCCGTGGc                                                                          >  1:829082/1‑138 (MQ=255)                                                             cATCGTTTTCATCTTTAAAGAAGAAAGCGCCATTGCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAGTAATAGCCCCGCGCACCACAGGAGTCGTTCCGAGTTGCACTTTATTCACCGTCGGTACGCCGTGGc                                                                          <  2:829082/138‑1 (MQ=255)                                                                     tCATCTTTAAAGAAGAAAGCGCCATTGCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAGTAATAGCCCCGCGCACCACAGGAGTCGTTCCGAGTTGCACTTTATTCACCGTCGGTACGCCGTGGCTTTCCAGCAc                                                                >  1:937144/1‑140 (MQ=255)                                                                      cATCTTTAAAGAAGAAAGCGCCATTGCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAGTAATAGCCCCGCGCACCACAGGAGTCGTTCCGAGTTGCACTTTATTCACCGTCGGTACGCCGTGGCTTTCCAGCAcc                                                               <  1:742312/140‑1 (MQ=255)                                                                       aTCTTTAAAGAAGAAAGCGCCATTGCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAGTAATAGCCCCGCGCACCACAGGAGTCGTTCCGAGTTGCACTTTATTCACCGTCGGTACGCCg                                                                              <  2:1344394/124‑1 (MQ=255)                                                                       aTCTTTAAAGAAGAAAGCGCCATTGCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAGTAATAGCCCCGCGCACCACAGGAGTCGTTCCGAGTTGCACTTTATTCACCGTCGGTACGCCg                                                                              >  1:1344394/1‑124 (MQ=255)                                                                            tAAAGAAGAAAGCGCCATTGCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAGTAATAGCCCCGCGCACCACAGGAGTCGTTCCGAGTTGCACTTTATTCACCGTCGGTACGCCGTGGCTTTCCAGCACCTGCaaa                                                         >  2:1087701/1‑140 (MQ=255)                                                                            tAAAGAAGAAAGCGCCATTGCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAGTAATAGCCCCGCGCACCACAGGAGTCGTTCCGAGTTGCACTTTATTCACCGTCGGTACGCCGTGGCTTTCCAGCACCTGCaaa                                                         >  2:487558/1‑140 (MQ=255)                                                                             aaaGAAGAAAGCGCCATTGCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAGTAATAGCCCCGCGCACCACAGGAGTCGTTCCGAGTTGCACTTTATTCACCGTCGGTACGCCGTGGCTTTCCAGCACCTGCaaaa                                                        <  1:1087701/140‑1 (MQ=255)                                                                                gaagaaAGCGCCATTGCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAGTAATAGCCCCGCGCACCGCAGGAGTCGTTCCGAGTTGCACTTTATTCACCGTCGGTACGCCGTGGCGTTCCAGCACCTGCAAAATGa                                                     <  2:272380/140‑1 (MQ=255)                                                                                   gaaAGCGCCATTGCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAGTAATAGCCCCGCGCACCACAGGAGTCGTTCCGAGTTGCACTTTATTCACCGTCGGTACGCCGTGGCTTTCCAGCACCTGCAAAATGatat                                                  >  1:304868/1‑140 (MQ=255)                                                                                      aGCGCCATTGCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAGTAATAGCCCCGCGCACCACAGGAGTCGTTCCGAGTTGCACTTTATTCACCGTCGGTACGCCGTGGCTTTCCAGCACCTGCAAAATGATATTGc                                               >  2:1538347/1‑140 (MQ=255)                                                                                         gcCATTGCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAGTAATAGCCCCGCGCACCACAGGAGTCGTTCCGAGTTGCACTTTATTCACCGTCGGTACGCCGTGGCTTTCCAGCACCTGCAAAATGATATTGCCGa                                            <  1:448748/140‑1 (MQ=255)                                                                                          ccATTGCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAGTAATAGCCCCGCGCACCACAGGAGTCGTTCCGAGTTGCACTTTATTCACCGTCGGTACGCCGTGGCTTTCCAGCACCTGCAAAATGATATTGCCGAg                                           <  2:652767/140‑1 (MQ=255)                                                                                          ccATTGCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAGTAATAGCCCCGCGCACCACAGGAGTCGTTCCGAGTTGCACTTTATTCACCGTCGGTACGCCGTGGCTTTCCAGCACCTGCAAAATGATATTGCCGAg                                           <  2:1347804/140‑1 (MQ=255)                                                                                          ccATTGCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAGTAATAGCCCCGCGCACCACAGGAGTCGTTCCGAGTTGCACTTTATTCACCGTCGGTACGCCGTGGCTTTCCAGCACCTGCAAAATGATATTGCCGAg                                           <  1:1247216/140‑1 (MQ=255)                                                                                           cATTGCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAGTAATAGCCCCGCGCACCACAGGAGTCGTTCCGAGTTGCACTTTATTCACCGTCGGTACGCCGTGGCTTTCCAGCACCTGCAAAATGATATTGCCGAGt                                          >  1:608122/1‑140 (MQ=255)                                                                                               gCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAGTAATAGCCCCGCGCACCACAGGAGTCGTTCCGAGTTGCACTTTATTCACCGTCGGTACGCCGTGGCTTTCCAGCACCTGCAAAATGATATTGCCGAGTAgcg                                      <  1:487558/140‑1 (MQ=255)                                                                                               gCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAGTAATAGCCCCGCGCACCACAGGAGTCGTTCCGAGTTGCACTTTATTCACCGTCGGTACGCCGTGGCTTTCCAGCACCTGCAAAATGATATTGCCGAGGAgcg                                      <  1:1538347/140‑1 (MQ=255)                                                                                                   gTATATTCCGGATAGATATCCAGTTCACCGGAAGTAATAGCCCCGCGCACCACAGGAGTCGTTCCGAGTTGCACTTTATTCACCGTCGGTACGCCGTGGCTTTCCAGCACCTGCAAAATGATATTGCCGAGTAGCGCAcc                                  <  2:937144/140‑1 (MQ=255)                                                                                                         tCCGGATAGATATCCAGTTCACCGGAAGTAATAGCCCCGCGCACCACAGGAGTCGTTCCGAGTTGCACTTTATTCACCGTCGGTACGCCGTGGCTTTCCAGCACCTGCAAAATGATATTGCCGAGTAGCGCACCtt                                <  2:983549/136‑1 (MQ=255)                                                                                                         tCCGGATAGATATCCAGTTCACCGGAAGTAATAGCCCCGCGCACCACAGGAGTCGTTCCGAGTTGCACTTTATTCACCGTCGGTACGCCGTGGCTTTCCAGCACCTGCAAAATGATATTGCCGAGTAGCGCACCtt                                >  1:983549/1‑136 (MQ=255)                                                                                                           cGGATAGATATCCAGTTCACCGGAAGTAATAGCCCCGCGCACCACAGGAGTCGTTCCGAGTTGCACTTTATTCACCGTCGGTACGCCGTGGCTTTCCAGCACCTGCAAAATGATATTGCCGAGTAGCGCACCTTCGGTAt                          >  1:914870/1‑140 (MQ=255)                                                                                                           cGGATAGATATCCAGTTCACCGGAAGTAATAGCCCCGCGCACCACAGGAGTCGTTCCGAGTTGCACTTTATTCACCGTCGGTACGCCGTGGCTTTCCAGCACCTGCAAAATGATATTGCCGAGTAGCGCACCTTCGGTAt                          <  1:216450/140‑1 (MQ=255)                                                                                                            ggATAGATATCCAGTTCACCGGAAGTAATAGCCCCGCGCACCACAGGAGTCGTTCCGAGTTGCACTTTATTCACCGTCGGTACGCCGTGGCTTTCCAGCACCTGCAAAATGATATTGCCGAGTAgcgc                                     >  2:888012/1‑128 (MQ=255)                                                                                                            ggATAGATATCCAGTTCACCGGAAGTAATAGCCCCGCGCACCACAGGAGTCGTTCCGAGTTGCACTTTATTCACCGTCGGTACGCCGTGGCTTTCCAGCACCTGCAAAATGATATTGCCGAGTAgcgc                                     <  1:888012/128‑1 (MQ=255)                                                                                                            ggATAGATATCCAGTTCACCGGAAGTAATAGCCCCGCGCACCACAGGAGTCGTTCCGAGTTGCACTTTATTCACCGTCGGTACGCCGTGGCTTTCCAGCACCTGCAAAATGATATTGCCGAGTAGCGCACCTTCGGTATc                         <  1:21369/140‑1 (MQ=255)                                                                                                                  atatCCAGTTCACCGGAAGTAATAGCCCCGCGCACCACAGGAGTCGTTCCGAGTTGCACTTTATTCACCGTCGGTACGCCGTGGCTTTCCAGCACCTGCAAAATGATATTGCCTAGTAGCGCACCTTCGGTATCGAtttt                   >  2:859425/1‑140 (MQ=255)                                                                                                                      ccAGTTCACCGGAAGTAATAGCCCCGCGCACCACAGGAGTCGTTCCGAGTTGCACTTTATTCACCGTCGGTACGCCGTGGCTTTCCAGCACCTGCAAAATGATATTGCCGAGTAGCGCACCTTCGGTATCGATTTTTGaa               <  2:1491052/140‑1 (MQ=255)                                                                                                                       cAGTTCACCGGAAGTAATAGCCCCGCGCACCACAGGAGTCGTTCCGAGTTGCACTTTATTCACCGTCGGTACGCCGTGGCTTTCCAGCACCTGCAAAATGATATTGCCGAGTAGCGCACCTTCGGTATCGATTTTTGAAc              >  2:1368012/1‑140 (MQ=255)                                                                                                                        aGTTCACCGGAAGTAATAGCCCCGCGCACCACAGGAGTCGTTCCGAGTTGCACTTTATTCACCGTCGGTACGCCGTGGCTTTCCAGCACCTGCAAAATGATATTGCCTAGTAGCGCACCTTCGGTATCGATTTTTGAAcc             <  1:859425/140‑1 (MQ=255)                                                                                                                            cACCGGAAGTAATAGCCCCGCGCACCACAGGAGTCGTTCCGAGTTGCACTTTATTCACCGTCGGTACGCCGTGGCTTTCCAGCACCTGCAAAATGATATTGCCGAGTAGCGCACCTTCGGTATCGATTTTTGAAc              >  2:329831/1‑135 (MQ=255)                                                                                                                            cACCGGAAGTAATAGCCCCGCGCACCACAGGAGTCGTTCCGAGTTGCACTTTATTCACCGTCGGTACGCCGTGGCTTTCCAGCACCTGCAAAATGATATTGCCGAGTAGCGCACCTTCGGTATCGATTTTTGAAc              <  1:329831/135‑1 (MQ=255)                                                                                                                            cACCGGAAGTAATAGCCCCGCGCACCACAGGAGTCGTTCCGAGTTGCACTTTATTCACCGTCGGTACGCCGTGGCTTTCCAGCACCTGCAAAATGATATTGCCGAGTAGCGCACCTTCGGTATCGATTTTTGAACCGACt         >  1:689783/1‑140 (MQ=255)                                                                                                                            cACCGGAAGTAATAGCCCCGCGCACCACAGGAGTCGTTCCGAGTTGCACTTTATTCACCGTCGGTACGCCGTGGCTTTCCAGCACCTGCAAAATGATATTGCCGAGTAGCGCACCTTCGGTATCGATTTTTGAACCGACt         >  2:1651735/1‑140 (MQ=255)                                                                                                                            cACCGGAAGTAATAGCCCCGCGCACCACAGGAGTCGTTCCGAGTTGCACTTTATTCACCGTCGGTACGCCGTGGCTTTCCAGCACCTGCAAAATGATATTGCCGAGTAGCGCACCTTCGGTATCGATTTTTGAACCGACt         >  2:1529984/1‑140 (MQ=255)                                                                                                                             aCCGGAAGTAATAGCCCCGCGCACCACAGGAGTCGTTCCGAGTTGCACTTTATTCACCGTCGGTACGCCGTGGCTTTCCAGCACCTGCAAAATGATATTGCCGAGTAGCGCACCTTCGGTATc                         <  1:100821/123‑1 (MQ=255)                                                                                                                             aCCGGAAGTAATAGCCCCGCGCACCACAGGAGTCGTTCCGAGTTGCACTTTATTCACCGTCGGTACGCCGTGGCTTTCCAGCACCTGCAAAATGATATTGCCGAGTAGCGCACCTTCGGTATc                         >  2:100821/1‑123 (MQ=255)                                                                                                                               cGGAAGTAATAGCCCCGCGCACCACAGGAGTCGTTCCGAGTTGCACTTTATTCACCGTCGGTACGCCGTGGCTTTCCAGCACCTGCAAAATGATATTGCCGAGTAGCGCACCTTCGGTATCGATTTTTGAACCGACTTTa      >  2:1780545/1‑140 (MQ=255)                                                                                                                                   aGTAATAGCCCCGCGCACCACAGGAGTCGTTCCGAGTTGCACTTTATTCACCGTCGGTACGCCGTGGCTTTCCAGCACCTGCAAAATGATATTGCCGAGTAGCGCACCTTCGGTATCGATTTTTGAACCGACTTTAACgg  <  1:1368012/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                      TGTGCTCCGAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCTGCATCGTTTTCATCTTTAAAGAAGAAAGCGCCATTGCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAGTAATCGCCCCGCGCACCACAGGAGTCGTTCCGAGTTGCACTTTATTCACCGTCGGTACGCCGTGGCTTTCCAGCACCTGCAAAATGATATTGCCGAGTAGCGCACCTTCGGTATCGATTTTTGAACCGACTTTAACGG  >  NC_000913/2219136‑2219405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑