BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,489,191	C→A	intergenic (‑23/+51)	yfdE ← / ← yfdV	acetyl‑CoA:oxalate CoA‑transferase/putative transport protein YfdV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,489,191	0	C	A	100.0%	373.6 / NA	104	intergenic (‑23/+51)	yfdE/yfdV	acetyl‑CoA:oxalate CoA‑transferase/putative transport protein YfdV
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (46/58); total (46/58)

Only 100 of 104 total aligned reads displayed.

TACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTGACGACAAAGCCCAGTACGCTCACAGCCAATGACGCTGTACC  >  NC_000913/2489055‑2489325

tACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGaa                                                                                                                                     <  2:253091/140‑1 (MQ=255)

tACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGaa                                                                                                                                     <  2:1743985/140‑1 (MQ=255)

   ccTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGt                                                                                                                                  >  2:339663/1‑140 (MQ=255)

      tGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGaa                                                                                                                               >  2:1099138/1‑140 (MQ=255)

       gcgcCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGaa                                                                                                                                     >  2:320682/1‑133 (MQ=255)

       gcgcCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGaa                                                                                                                                     <  1:320682/133‑1 (MQ=255)

          cccATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAg                                                                                                                            >  2:212052/1‑139 (MQ=255)

          cccATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAg                                                                                                                            <  1:212052/139‑1 (MQ=255)

          cccATATTACAAAGAAGTTGAGTTACGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCAATATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGa                                                                                                                           <  2:1602484/140‑1 (MQ=255)

             atatTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGacac                                                                                                                        >  1:1378683/1‑140 (MQ=255)

                ttACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTc                                                                                                                     <  1:1099138/140‑1 (MQ=255)

                  aCAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGaa                                                                                                                                     <  2:1534622/122‑1 (MQ=255)

                  aCAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGaa                                                                                                                                     >  1:1534622/1‑122 (MQ=255)

                     aagaagTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCAt                                                                                                                <  2:1378683/140‑1 (MQ=255)

                     aagaagTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCAt                                                                                                                >  1:500312/1‑140 (MQ=255)

                      agaagTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCAtt                                                                                                               <  1:1071076/140‑1 (MQ=255)

                        aagTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTa                                                                                                             >  1:1470703/1‑140 (MQ=255)

                        aagTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTa                                                                                                             >  1:1582651/1‑140 (MQ=255)

                        aagTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTa                                                                                                             >  2:840560/1‑140 (MQ=255)

                          gTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGa                                                                                                                           >  1:435573/1‑124 (MQ=255)

                          gTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGa                                                                                                                           <  2:435573/124‑1 (MQ=255)

                          gTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATg                                                                                                           >  2:1099578/1‑140 (MQ=255)

                          gTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATg                                                                                                           >  1:455128/1‑140 (MQ=255)

                            tGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTa                                                                                                         <  1:295717/140‑1 (MQ=255)

                                ttCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAAt                                                                                                     >  2:1318006/1‑140 (MQ=255)

                                  ccGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCa                                                                                                   >  2:355516/1‑140 (MQ=255)

                                    gAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAAt                                                                                                 <  1:1514926/140‑1 (MQ=255)

                                    gAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAAt                                                                                                 <  2:455128/140‑1 (MQ=255)

                                         gTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACAt                                                                                            >  2:1187010/1‑140 (MQ=255)

                                         gTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACAt                                                                                            <  1:355516/140‑1 (MQ=255)

                                          tCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATg                                                                                           >  1:1504760/1‑140 (MQ=255)

                                          tCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATg                                                                                           >  2:13350/1‑140 (MQ=255)

                                          tCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATg                                                                                           >  1:260013/1‑140 (MQ=255)

                                           ccATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGa                                                                                          <  1:1470725/140‑1 (MQ=255)

                                            cATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGat                                                                                         <  1:906332/140‑1 (MQ=255)

                                             aTTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGatt                                                                                        <  1:444073/140‑1 (MQ=255)

                                              ttAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGatta                                                                                       <  1:1187010/140‑1 (MQ=255)

                                                    aCATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATg                                                                                 >  1:1289801/1‑140 (MQ=255)

                                                      aTGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGaa                                                                               >  1:181484/1‑140 (MQ=255)

                                                        gtgtCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAAc                                                                             >  2:530478/1‑140 (MQ=255)

                                                           tCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAg                                                                          >  2:812388/1‑140 (MQ=255)

                                                            cATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGt                                                                         <  2:1252913/140‑1 (MQ=255)

                                                             atatCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTc                                                                        >  2:1462200/1‑140 (MQ=255)

                                                             atatCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTc                                                                        >  2:1460048/1‑140 (MQ=255)

                                                               atCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGa                                                                      <  1:644696/140‑1 (MQ=255)

                                                                tCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGAc                                                                     <  2:708686/140‑1 (MQ=255)

                                                                tCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGAc                                                                     <  2:146498/140‑1 (MQ=255)

                                                                tCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGAc                                                                     >  2:122371/1‑140 (MQ=255)

                                                                 cGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACt                                                                    <  2:206726/140‑1 (MQ=255)

                                                                 cGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACt                                                                    <  2:1470703/140‑1 (MQ=255)

                                                                 cGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACt                                                                    <  1:122371/140‑1 (MQ=255)

                                                                   aTAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGa                                                                  >  2:34315/1‑140 (MQ=255)

                                                                   aTAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGa                                                                  >  1:883322/1‑140 (MQ=255)

                                                                     aaCTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACa                                                                >  1:1213637/1‑140 (MQ=255)

                                                                        taataaGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATaa                                                             <  1:34315/140‑1 (MQ=255)

                                                                          ataaGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAAt                                                           >  1:900376/1‑140 (MQ=255)

                                                                          ataaGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAAt                                                           >  1:1781926/1‑140 (MQ=255)

                                                                          ataaGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAAt                                                           >  1:1809310/1‑140 (MQ=255)

                                                                                  tCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATa                                                              >  2:1216147/1‑129 (MQ=255)

                                                                                  tCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATa                                                              <  1:1216147/129‑1 (MQ=255)

                                                                                    aaaCGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGa                                                 <  2:1416648/140‑1 (MQ=255)

                                                                                    aaaCGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGACGACTGACATAAATCCACAAGGGa                                                 <  1:284698/140‑1 (MQ=255)

                                                                                     aaCGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAg                                                <  2:1289801/140‑1 (MQ=255)

                                                                                         gCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCca                                                        <  2:298377/128‑1 (MQ=255)

                                                                                         gCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCca                                                        >  1:298377/1‑128 (MQ=255)

                                                                                          cccTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTg                                           >  2:1179750/1‑140 (MQ=255)

                                                                                              ttGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTgacga                                       <  1:1099578/140‑1 (MQ=255)

                                                                                                    tCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTGACGACAAAGc                                 >  1:358148/1‑140 (MQ=255)

                                                                                                      attattTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTGACGACAAAGccc                               <  2:1582651/140‑1 (MQ=255)

                                                                                                         attTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTGACGACAAAGCCCAGt                            <  2:1213637/140‑1 (MQ=255)

                                                                                                         attTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTGACGACAAAGCCCAGt                            <  1:530478/140‑1 (MQ=255)

                                                                                                          tttGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTGACGACAAAGACCAGTa                           <  1:339663/140‑1 (MQ=255)

                                                                                                           ttGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTGACGACAAAGCCCAg                             >  2:546926/1‑137 (MQ=255)

                                                                                                           ttGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTGACGACAAAGCCCAg                             <  1:546926/137‑1 (MQ=255)

                                                                                                            tGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTGACGACAAAGCCCAGTACg                         >  2:895652/1‑140 (MQ=255)

                                                                                                              tCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTGACGACAAAGCCCAGTACGCt                       <  2:1809310/140‑1 (MQ=255)

                                                                                                                aTTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTGACGACAAAGCCCAGTACGCTca                     >  2:1212134/1‑140 (MQ=255)

                                                                                                                aTTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTGACGACAAAGCCCAGTACGCTca                     <  1:1318006/140‑1 (MQ=255)

                                                                                                                        aTGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTGACGACAAAGCCCAGTACGCTCACAGCCAAt             >  1:659274/1‑140 (MQ=255)

                                                                                                                          gCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTGACGACAAAGCCCAGTACGCTCACAGCCAATGa           >  1:853002/1‑140 (MQ=255)

                                                                                                                          gCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTGACGACAAAGCCCAGTACGCTCACAGCCAATGa           <  1:15721/140‑1 (MQ=255)

                                                                                                                          gCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTGACGACAAAGCCCAGTACGCTCACAGCCAATGa           <  2:500312/140‑1 (MQ=255)

                                                                                                                            cTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTGACGACAAAGCCCAGTACGCTCACAGCCAATGACg         <  1:609359/140‑1 (MQ=255)

                                                                                                                            cTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTGACGACAAAGCCCAGTACGCTCACAGCCAATGACg         <  1:1462200/140‑1 (MQ=255)

                                                                                                                            cTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTGACGACAAAGCCCAGTACGCTCACAGCCAATGACg         <  1:29618/140‑1 (MQ=255)

                                                                                                                            cTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTGACGACAAAGCCCAGTACGCTCACAGCCAATGACg         <  1:895652/140‑1 (MQ=255)

                                                                                                                            cTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTGACGACAAAGCCCAGTACGCTCACAGCCAATGACg         <  2:1504760/140‑1 (MQ=255)

                                                                                                                            cTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTGACGACAAAGCCCAGTACGCTCACAGCCAATGACg         <  2:659274/140‑1 (MQ=255)

                                                                                                                            cTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTGACGACAAAGCCCAGTACGCTCACAGCCAATGACg         <  2:853002/140‑1 (MQ=255)

                                                                                                                            cTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTGACGACAAAGCCCAGTACGCTCACAGCCAATGACg         <  2:883322/140‑1 (MQ=255)

                                                                                                                            cTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTAAGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTGACGACAAAGCCCAGTACGCTCACAGCCAATGACg         <  2:260013/140‑1 (MQ=255)

                                                                                                                             ttGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTGACGACAAAGCCCAGTACGCTCACAGCCAATGACGc        <  1:840560/140‑1 (MQ=255)

                                                                                                                              tGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTGACGACAAAGCCCAGTACGCTCACAGCCAATGACGCt       >  2:1776722/1‑140 (MQ=255)

                                                                                                                              tGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTGACGACAAAGCCCAGTACGCTCACAGCCAATGACGCt       >  2:375396/1‑140 (MQ=255)

                                                                                                                               gAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTGACGACAAAGCCCAGTACGCTCACAGCCAAt             <  2:152662/133‑1 (MQ=255)

                                                                                                                               gAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTGACGACAAAGCCCAGTACGCTCACAGCCAAt             >  1:152662/1‑133 (MQ=255)

                                                                                                                               gAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTGACGACAAAGCCCAGTACGCTCACAGCCAATGACGCTg      >  2:780015/1‑140 (MQ=255)

                                                                                                                               gAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTGACGACAAAGCCCAGTACGCTCACAGCCAATGACGCTg      >  1:1742083/1‑140 (MQ=255)

                                                                                                                                  aaaaGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTGACGACAAAGCCCAGTACGCTCACAGCCAATGACGCTGTAc   <  2:181484/140‑1 (MQ=255)

                                                                                                                                   aaaGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTGACGACAAAGCCCAGTACGCTCACAGCCAATGACGCTGTAcc  <  1:1212134/140‑1 (MQ=255)

TACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTGACGACAAAGCCCAGTACGCTCACAGCCAATGACGCTGTACC  >  NC_000913/2489055‑2489325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`