Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,274,865	0	T	G	22.4%	134.5 / 11.8	58	T15P (ACC→CCC)	garP	galactarate/glucarate/glycerate transporter GarP
Reads supporting (aligned to +/- strand):  ref base T (14/31);  new base G (13/0);  total (27/31)
Fisher's exact test for biased strand distribution p-value = 6.36e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ATCGAAACCGCACTTAACTGCAACTCTTTTGCCACTTCGGTACCAGCAATAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTCGGGAA  >  NC_000913/3274744‑3275003                                                                                                                          |                                                                                                                                           aTCGAAACCGCACTTAACTGCAACTCTTTTGCCACTTCGGTACCAGCAATAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAAAAATCAATAATATTTAAAAGCGGGGACGCACGCCCCTCctttt                                                                                                                          >  1:46011/1‑140 (MQ=255)  tCGAAACCGCACTTAACTGCAACTCTTTTGCCACTTCGGTACCAGCAATAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGCGGGTATGCACGCCTTTCtttttt                                                                                                                         >  1:620211/1‑140 (MQ=255)  tCGAAACCGCACTTAACTGCAACTCTTTTGCCACTTCGGTACCAGCAATAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGCGGGGATGCACGCCTTTCtttttt                                                                                                                         >  2:307269/1‑140 (MQ=255)  tCGAAACCGCACTTAACTGCAACTCTTTTGCCACTTCGGTACCAGCAATAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGCGGGGATGCACGCCTTTCtttttt                                                                                                                         >  1:1282386/1‑140 (MQ=255)  tCGAAACCGCACTTAACTGCAACTCTTTTGCCACTTCGGTACCAGCAATAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGGTAAAAGAAAAATAATCAGTAATATTTAATACCAGGTCGGCACGCCCTtttttttt                                                                                                                         >  2:694656/1‑140 (MQ=255)      aaCCGCACTTAACTGCAACTCTTTTGCCACTTCGGTACCAGCAATAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGt                                                                                                                     <  1:307269/140‑1 (MQ=255)         cGCACTTAACTGCAACTCTTTTGCCACTTCGGTACCAGCAATAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCaa                                                                                                                  <  1:1172153/140‑1 (MQ=255)          gCACTTAACTGCAACTCTTTTGCCACTTCGGTACCAGCAATAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAAc                                                                                                                 <  1:894343/140‑1 (MQ=255)          gCACTTAACTGCAACTCTTTTGCCACTTCGGTACCAGCAATAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAACGGGGATGCACGCCTTTTTTTTTTTTGTCAAc                                                                                                                 >  2:938878/1‑140 (MQ=255)            aCTTAACTGCAACTCTTTTGCCACTTCGGTACCAGCAATAGACAGCGTTGCACGAACGGCGTAGTTAACGGCGGAACCAATCAAAAAAATCAACAACATTTAAAACCGGGTGTCAAAGCCTTTCTTTTTTTTTTCAACgg                                                                                                               >  2:895685/1‑140 (MQ=255)              ttAACTGCAACTCTTTTGCCACTTCGGTACCAGCAATAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGgtg                                                                                                             <  1:1388435/140‑1 (MQ=255)                aaCTGCAACTCTTTTGCCACTTCGGTACCAGCAATAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGt                                                                                                                     <  2:195621/130‑1 (MQ=255)                aaCTGCAACTCTTTTGCCACTTCGGTACCAGCAATAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGCGGGGATGCACGCCTTTCTTTTTTTCGt                                                                                                                     >  1:195621/1‑130 (MQ=255)                   tGCAACTCTTTTGCCACTTCGGTACCAGCAATAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAg                                                                                                        <  1:1447492/140‑1 (MQ=255)                    gCAACTCTTTTGCCACTTCGGTACCAGCAATAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGa                                                                                                       <  2:161558/140‑1 (MQ=255)                     cAACTCTTTTGCCACTTCGGTACCAGCAATAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGaa                                                                                                      <  2:363120/140‑1 (MQ=255)                     cAACTCTTTTGCCACTTCGGTACCAACAATAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAAAAATTAGTAATATTAAATAACGGGGATGCACGCCTTTTTTTTTTTTTTCAACGGTGTCCAGaa                                                                                                      >  2:1224795/1‑140 (MQ=255)                        ctctTTTGCCACTTCGGTACCAGCAATAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAAAAATCAGTAATATTAAATAGCGGGGATGCACGCCTTTCTTTTTTTTGTCAACGGTGTCCAGAATCa                                                                                                   >  1:769555/1‑140 (MQ=255)                            tttGCCACTTCGGTACCAGCAATAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGAAAAAATAAAAAAAATGAAAAAAAATAAATCTCGGGTATTGACGCCTTTATTTTTTTTGTGCACCGGGTGCAGAATCAtttt                                                                                               >  2:1441270/1‑140 (MQ=255)                                  aCTTCGGTACCAGCAATAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTAc                                                                                         <  2:315582/140‑1 (MQ=255)                                  aCTTCGGTACCAGCAATAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTAc                                                                                         <  1:641388/140‑1 (MQ=255)                                   cTTCGGTACCAGCAATAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTAcc                                                                                        <  1:482071/140‑1 (MQ=255)                                   cTTCGGTACCAGCAATAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTAcc                                                                                        <  2:1053426/140‑1 (MQ=255)                                   cTTCGGTACCAGCAATAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTAcc                                                                                        <  2:25897/140‑1 (MQ=255)                                                     aCAGCGTTGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGAt                                                                      <  1:1441270/140‑1 (MQ=255)                                                         tgTTGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAAAAATCAGTAATATTAAATAGCGGGGATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGAttttt                                                                  >  2:383397/2‑140 (MQ=255)                                                         tgTTGCACGATCGGCGTAGTTAACGGCGGGAACAATAAAAAAAATCAGTAATTTTTAATATGGGGGATGCGCGCCTTTCTTTTTTTTGTCAACGGGGTTCCGAATCATTTTTTTTTCCTCGGGGGGTTATGCTGAttttt                                                                  >  2:682598/2‑140 (MQ=255)                                                         cGTTGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGCGGGGATGCACGCCTTTCTTTTTTTTGTCAACGGGGTCCAGAATCATTTTATTTAACTCGGGGACTTTTGCTGAttttt                                                                  >  2:526482/1‑140 (MQ=255)                                                         cGTTGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGCGGGGATGCACGCCCTTCTTTTTTTTGTCAACGGGGTCCAGAATCATTTTATTTACCTCGGGGACTTATGCTGAttttt                                                                  >  2:663961/1‑140 (MQ=255)                                                         cGTTGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAAAAATCAGTAATATTAAATAGCGGGGATGCACGCCCTTTTTTTTTTTGTCAACCGGGTCCCGAATCATTTTATTTTCCTCGGGGACTTTTGCTGAttttt                                                                  >  2:829448/1‑140 (MQ=255)                                                          gTTGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTtta                                                                 <  1:829448/140‑1 (MQ=255)                                                            tGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGCGGGGATGCACGCCCTTTTTTTTTTTGTCAACGGGGTCCAGAATCATTTTATTTACCTCGGGGACTTTTTCTGAtttttttt                                                               >  2:562010/1‑140 (MQ=255)                                                            tGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAAAAATTAGTAATATTAAATAGCGGGGATGGACGCCTTTCTTTTTTTTGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTTTGCTGATTTttatt                                                               >  2:232282/1‑140 (MQ=255)                                                            tGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAAAAATCAGTAATATTAAATAGCGGGGATGCACGCCTTTCTTTTTTTTGTCAACGGGGTCCAGAATCATTTTTTTTTCCTCGGGGACTTATGCTGAtttttttt                                                               >  2:157377/1‑140 (MQ=255)                                                            tGCACGATCGGCGTAGTTAACGGCGGTAACAAAAAAAATAATCAGTAATATTAAATAGCGGGGATGCGCGCCTTTCTTTTTTTTTTCAACGGTGTCCCGAATAATTTTATTTTACTCGGGTAATTTTTCTGAtttttttt                                                               >  2:1319576/1‑140 (MQ=255)                                                              cACGATCGGCGTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTttattat                                                             <  2:620211/140‑1 (MQ=255)                                                                   tCGGCGTAGTTAACGGCGGTAACAAAAAAAAAAATCAGTAATATTAAATAGCGGGGATGCACGCCCTTCTTTTTTTTGTCAACGGTGTCCAGAAACATTTTATTTACCTCGGGTACTTATGCTGATTTTtttttttattg                                                        >  2:1460889/1‑138 (MQ=255)                                                                     ggCGTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATgggg                                                      <  1:1437666/140‑1 (MQ=255)                                                                      gCGTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGa                                                     <  1:1039599/140‑1 (MQ=255)                                                                      gCGTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGCGGGGATGCACGCCTTTCTTTTTTTTGTCAACGGTGTCCAGAATCATTTTATTTTCCTCGGGGACTTATGCTGATTTTTTTTATTTTggggg                                                     >  1:1458685/1‑139 (MQ=255)                                                                         tAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAgg                                                  <  1:526482/140‑1 (MQ=255)                                                                           gTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGgtg                                                <  1:654166/140‑1 (MQ=255)                                                                            ttAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGgtgt                                               <  2:1282386/140‑1 (MQ=255)                                                                                cggcggTAACAATAAAAATAATCAGTAATATTAAATAGCGGGGATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTTCTTCGGGTACTTATGCTGATTTTTTTTTTTATGGGGGAGGGGttatt                                           >  2:1391648/1‑140 (MQ=255)                                                                                  gcggTAACAATAAAAATAATCAGTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGttattta                                         <  2:1132694/140‑1 (MQ=255)                                                                                   cggTAACAATAAAAATAATCAGTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGttatttat                                        <  2:1120468/140‑1 (MQ=255)                                                                                     gTAACAATAAAAATAATCAGTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGaa                                                    <  2:558194/126‑1 (MQ=255)                                                                                     gTAACAATAAAAATAATCAGTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGaa                                                    >  1:558194/1‑126 (MQ=255)                                                                                            tAAAAATAATCAGTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCAt                               >  1:1409900/1‑140 (MQ=255)                                                                                                aataatCAGTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTAt                           <  1:938878/140‑1 (MQ=255)                                                                                                aataatCAGTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTAt                           >  1:304721/1‑140 (MQ=255)                                                                                                aataatCAGTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTAt                           <  1:1224795/140‑1 (MQ=255)                                                                                                        gTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAAc                   >  2:741798/1‑140 (MQ=255)                                                                                                                     gCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTCg      >  1:184862/1‑140 (MQ=255)                                                                                                                      cGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGTTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTCgg     >  1:579475/1‑140 (MQ=255)                                                                                                                      cGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTCgg     >  2:1011261/1‑140 (MQ=255)                                                                                                                      cGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTCgg     >  2:1353909/1‑140 (MQ=255)                                                                                                                      cGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTCgg     <  2:227868/140‑1 (MQ=255)                                                                                                                      cGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTCgg     >  2:758195/1‑140 (MQ=255)                                                                                                                      cGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTCgg     >  2:128726/1‑140 (MQ=255)                                                                                                                      cGGGTATACACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTCgg     >  2:74213/1‑140 (MQ=255)                                                                                                                       gggTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTCggg    >  2:868109/1‑140 (MQ=255)                                                                                                                        ggTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTCGGGa   >  1:67333/1‑140 (MQ=255)                                                                                                                         gTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGTCGTAACGACAATGAACTCGGGaa  <  1:895685/140‑1 (MQ=255)                                                                                                                         gTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTCGGGaa  <  1:1299300/140‑1 (MQ=255)                                                                                                                         gTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTCGGGaa  <  1:1193242/140‑1 (MQ=255)                                                                                                                         gTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTCGGGaa  <  2:46011/140‑1 (MQ=255)                                                                                                                         gTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTCGGGaa  <  1:1353909/140‑1 (MQ=255)                                                                                                                          |                                                                                                                                           ATCGAAACCGCACTTAACTGCAACTCTTTTGCCACTTCGGTACCAGCAATAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTCGGGAA  >  NC_000913/3274744‑3275003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑