Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913372,6400TG32.3% 48.0 / 11.3 31L176V (TTA→GTA) mhpD2‑hydroxypentadienoate hydratase
Reads supporting (aligned to +/- strand):  ref base T (5/16);  new base G (10/0);  total (15/16)
Fisher's exact test for biased strand distribution p-value = 6.77e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TTGAATGGGTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAACCGCT  >  NC_000913/372503‑372779
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ttGAATGGGTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTa                                                                                                                                           >  1:961219/1‑140 (MQ=255)
  gAATGGGTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGa                                                                                                                                         <  2:263252/140‑1 (MQ=255)
        gTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGaa                                                                                                                                   >  1:640051/1‑140 (MQ=255)
        gTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTTGAcccgga                                                                                                                                   >  2:333746/1‑136 (MQ=255)
        gTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGACCTGaa                                                                                                                                   >  2:553119/1‑140 (MQ=255)
                ggCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGACCTGAAAAACTgcg                                                                                                                           >  1:34180/1‑140 (MQ=255)
                    cTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCAt                                                                                                                       <  2:878078/140‑1 (MQ=255)
                      ggAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCCACGTCCGGCGGGGGTAGGCCTGAAAAAATGCGCCCTGa                                                                                                                     >  2:113649/1‑140 (MQ=255)
                      ggAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTgcgc                                                                                                                          <  2:778599/135‑1 (MQ=255)
                      ggAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGACCTGAAAAACTgcgc                                                                                                                          >  1:778599/1‑135 (MQ=255)
                         aGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGa                                                                                                                  <  1:688528/140‑1 (MQ=255)
                            ggtggGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGa                                                                                                               <  1:113649/140‑1 (MQ=255)
                              tggGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGGGGGGGTTGACCCGGAAAACTGCGCCCTTAAGATGAcg                                                                                                             >  2:49692/1‑140 (MQ=255)
                                          ttCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGaa                                                                                                 <  1:780814/140‑1 (MQ=255)
                                          ttCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGaa                                                                                                 <  1:497142/140‑1 (MQ=255)
                                                    gTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTTGACCTGAAAAACTGCGCCATGAAGGAGGCGCGTAATAACGAAGAGGGTTCTa                                                                                       >  1:466117/1‑140 (MQ=255)
                                                     tCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAg                                                                                      >  1:122013/1‑140 (MQ=255)
                                                          tCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGgc                                                                                 <  2:523174/140‑1 (MQ=255)
                                                              tttGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCgg                                                                             <  1:925232/140‑1 (MQ=255)
                                                                   cGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCg                                                                        <  2:527822/140‑1 (MQ=255)
                                                                   cGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGGTTCTAGCGGGCGCGGCAGCg                                                                        >  1:199332/1‑140 (MQ=255)
                                                                     aTACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGaa                                                                      <  2:286906/140‑1 (MQ=255)
                                                                         cGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGcc                                                                  >  2:676610/1‑140 (MQ=255)
                                                                            ggCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGa                                                                       >  1:565737/1‑132 (MQ=255)
                                                                            ggCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGa                                                                       <  2:565737/132‑1 (MQ=255)
                                                                                  tAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTTGACCCGAAAAACTGCACCATGAAGAGGACGCGGAATAACGGAGAGGATTCTAGCGGGCGCGGCCGCGAAAGCCTTGGGCATc                                                         >  2:75588/1‑140 (MQ=255)
                                                                                              tgGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCgg                                             <  1:551539/140‑1 (MQ=255)
                                                                                              tgGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCgg                                             >  1:560746/1‑140 (MQ=255)
                                                                                                  gtgtATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGt                                         <  1:939647/140‑1 (MQ=255)
                                                                                                   tgtATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGACCTGAAAAACTGCGCCATGAAGATGACGCGGAATAAAGAAGAGGGTTCTAGCGGGCGCGGCAGCGAAAGCCTGGGACATCCCCTTAATGCGGCCggc                                        >  2:551539/1‑138 (MQ=255)
                                                                                                     tATGTCATCGGCGGTCCGGCGCAACGTGCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGGGAATGCCTGGGGCATCCGCTTAATGGGGCCGTctg                                      >  2:772061/1‑140 (MQ=255)
                                                                                                     tATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGGTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTctg                                      >  1:949375/1‑140 (MQ=255)
                                                                                                             cggcggTCCGGCGCAACGTCCGGCGGGGGTAGACCTGAAAAACTGCGCCATGAAGATGACGCGGAATAACGAAGAGGGTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCAc                              >  2:591460/1‑140 (MQ=255)
                                                                                                             cggcggTCCGGCGCAACGTCCGGCGGGGGTAGACCTGAAAAACTGCGCCATGAAGAAGACGCGTAATAACGAAGAGGGTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCAc                              >  2:466551/1‑140 (MQ=255)
                                                                                                               gcggTCCGGCGCAACGTCCGGCGGGGGTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGGTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGc                            >  1:751976/1‑140 (MQ=255)
                                                                                                                     cGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATg                      <  1:676610/140‑1 (MQ=255)
                                                                                                                       gcgcAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGc                    <  2:560746/140‑1 (MQ=255)
                                                                                                                                         ttAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAACCGCt  <  2:311990/140‑1 (MQ=255)
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TTGAATGGGTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAACCGCT  >  NC_000913/372503‑372779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑