Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913445,5580AC42.3% 18.2 / 21.9 26V370G (GTA→GGA) yajRputative major facilitator superfamily transport protein YajR
Reads supporting (aligned to +/- strand):  ref base A (9/6);  new base C (0/11);  total (9/17)
Fisher's exact test for biased strand distribution p-value = 2.41e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGT  >  NC_000913/445420‑445692
                                                                                                                                          |                                                                                                                                      
aGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATAc                                                                                                                                       >  1:778090/1‑140 (MQ=255)
   aaaCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTTATGGGACTGGCGACTGTCAGCCACACTGCGGCCCGCATTTCGCCCGCGAGAAATAaccc                                                                                                                                    >  2:423267/1‑140 (MQ=255)
    aaCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATAccccc                                                                                                                                   <  1:423267/140‑1 (MQ=255)
     aCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCCCACTGCGGCCCGCATTGCGCCAGCGAGAAAAAcccccc                                                                                                                                  >  2:487163/1‑139 (MQ=255)
     aCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCt                                                                                                                                  <  2:193763/140‑1 (MQ=255)
       gCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGa                                                                                                                                <  1:531443/140‑1 (MQ=255)
                  gCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACAt                                                                                                                     <  2:350648/140‑1 (MQ=255)
                        tttGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGtt                                                                                                               <  2:874941/140‑1 (MQ=255)
                          tGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTaa                                                                                                             <  2:314277/140‑1 (MQ=255)
                                                     gcgcAAACTGCTGACATACGGCGGTTCTTTTCTGGTACTGGCGACTGTCAGCCCCACTGCGGCCCGCATTGCGCCAGCGAGAAAAACCCCCCGACCGTCAAAAATGCCGTTTATCCAGCCGCCCCGCGAACCCCCCATcc                                                                                  >  2:528500/1‑139 (MQ=255)
                                                      cgcAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCCGCATTGCGCCAGCGAGAAATAACCCCCTACCGTCAAACATGCCGTTAATCCAACCGCCCCGCGAACCGCCCAAcgc                                                                                 >  2:475901/1‑140 (MQ=255)
                                                        cAAACTGCTGACATACGGCGGTTTTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATcgcca                                                                               <  2:778090/140‑1 (MQ=255)
                                                                    atccggcgtTTCTTCCAGGGTACTGGCGATTGTCAGCCACCCGGCGGCAAGCATGGCGCAAGCGAAAAATCCCCCCTGCCCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAAct                                                                   <  2:959495/137‑1 (MQ=255)
                                                                            gTTCTTTCATGGGACTGGCGACTGTCAGCCACACCGCGGCCAGCAATGCGCCAGCGAGAAATAACCCCCGACCCTCAAACATGCCGGTAATTCAGCCCCCCCGCGAACCCCCCAACGCCCCGCCCAAAAAacggctgggg                                                           >  2:872209/1‑138 (MQ=255)
                                                                                tttCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGt                                                       <  1:269819/140‑1 (MQ=255)
                                                                                  tCATGGTACGGGCGCCTGTCAGCCACCCTGCGGCCAGCATTGCGCCAGCGAAAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTaa                                                     <  1:872209/140‑1 (MQ=255)
                                                                                           tGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATc                                            >  2:439147/1‑140 (MQ=255)
                                                                                           tGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATc                                            >  2:947094/1‑140 (MQ=255)
                                                                                             gcgcCTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAAAAATCCCCCCTTCCCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGc                                          <  1:475901/140‑1 (MQ=255)
                                                                                                 cTGTCAGCCACCCTGCGGCCAGCATTGCGCCAGGGAAAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTa                                      <  1:487163/140‑1 (MQ=255)
                                                                                                 cTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTa                                      <  1:55526/140‑1 (MQ=255)
                                                                                                         cacaCGGCGGCCAGCATTGCGCCGGCGAAAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTa                              <  1:528500/140‑1 (MQ=255)
                                                                                                              tGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACctg                         >  2:565358/1‑140 (MQ=255)
                                                                                                                     aGCATTGCGCCAGCGAAAAATCCCCCCTGCCCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGa                  <  1:263064/140‑1 (MQ=255)
                                                                                                                     aGCATGGCGCCAGCGAGAAATCCCCCCTGACCGCCAAAAATCCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGa                  <  1:447218/140‑1 (MQ=255)
                                                                                                                      gCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGAc                 <  1:941601/140‑1 (MQ=255)
                                                                                                                      gCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGAc                 >  1:891793/1‑140 (MQ=255)
                                                                                                                        aTTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGActc               >  1:334691/1‑140 (MQ=255)
                                                                                                                         ttGCGCCAGCGAAAAATCCCCCCTGCCCGTCAAACATGCCGTTAACCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGActct              <  2:334691/140‑1 (MQ=255)
                                                                                                                           gcgcCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCttt            <  1:565358/140‑1 (MQ=255)
                                                                                                                            cgcCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCttt            <  1:180968/139‑1 (MQ=255)
                                                                                                                            cgcCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCttt            >  2:180968/1‑139 (MQ=255)
                                                                                                                            cgcCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTa           >  1:30793/1‑140 (MQ=255)
                                                                                                                            ccccAGCAAAAAATCCCCCCTGCCCGTAAACCATGCCGTTAACCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTa           <  2:836569/138‑1 (MQ=255)
                                                                                                                                 gCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGAt      >  2:359679/1‑140 (MQ=255)
                                                                                                                                 gCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGAt      >  1:836569/1‑140 (MQ=255)
                                                                                                                                     aaaaTCCCCCCTGCCCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGt  <  2:30793/139‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                      
AGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGT  >  NC_000913/445420‑445692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: