| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 650,809 | 0 | T | G | 45.8% | 15.0 / 13.7 | 24 | T350P (ACC→CCC) | citC | citrate lyase synthetase |
| Reads supporting (aligned to +/- strand): ref base T (6/7); new base G (11/0); total (17/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.91e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGC‑AGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGCGG > NC_000913/650670‑650943 | ctcAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTTATTTTTCACCTGTTTCTCATGCGGGGGt < 2:718547/140‑1 (MQ=255) ttGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTTATTTTTTCCCTGTTTCTCATGCGGGGGGCTTTTTACGAGCTGCcgc > 2:839258/1‑140 (MQ=255) gCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGt > 1:385754/1‑140 (MQ=255) aGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTcc < 1:201449/140‑1 (MQ=255) ggTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTTACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTccc > 2:511231/1‑139 (MQ=255) cGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTTACCTGTTTCTCATGCGGGGGGCTTTTTACGAGCTGCCGCGTCCTGGc > 2:454739/1‑140 (MQ=255) ccTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTATTTTCATTTTTCACCTGTTTCTCACGCGGGGGGCTTTTTGCGAGCTGCCGCGTCCTGGCGGGCGt > 1:224301/1‑140 (MQ=255) tGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCgggggggc > 1:419811/1‑135 (MQ=255) gCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCt > 1:122720/1‑140 (MQ=255) tCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGc < 2:385754/140‑1 (MQ=255) ggCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTATTTTCATTTTTCACCCGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGCTCAAAC‑AGGGTCtt > 2:37296/1‑139 (MQ=255) tATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGTGGTCCAGC‑AGGGTCTTCCaataata > 2:483375/1‑139 (MQ=255) acaTCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGC‑AGGTTCTGCAAATAATGCAGCGTg < 1:483375/140‑1 (MQ=255) caTCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTTTTTTCATTTTTTACCTGTTTCTCATGGGGGGGGCTTTTTACGAGCTGCCGCGTCCTGGCGGGGGGGGTCAAGCAAGGTTTTCCAAA‑AATGCAGGGTgg > 2:814047/1‑139 (MQ=255) ccccAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGC‑AGGTTCTGCAAATAATGCAGCGTGACTg > 1:329988/1‑140 (MQ=255) ccAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGC‑AGGTTCTGCAAATAATGCAGCGTGACTGCa < 1:97025/140‑1 (MQ=255) gACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGGTCAAGC‑AGGTTCTGCCAATAATGCAGCGTGACTGCAggg > 1:273564/1‑140 (MQ=255) aaGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCCCCCGTTTCTCATGCGGGGGGCCTTTTACTCGCTGCCGCGTCCTGGCGGGGGGGCCCCAAC‑CGGGTCTTCAAAAAATTCAGCGGGACTGCAcggggcca > 2:97025/1‑135 (MQ=255) gggTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGCTCAAAC‑AGGGTTTTCAAAAAAAGCAACGTGACTGCAGGGGCCcgcg > 2:755784/1‑140 (MQ=255) tGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGC‑AGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAgcggcg < 2:122720/140‑1 (MQ=255) gCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGTGCTCAAGC‑AGGGTCTGCAAATAATGCAGCGTGGCTGCAGGGACCCGCGgcgc > 2:95198/1‑140 (MQ=255) ccTGCAACGGCGGGCTGGGTTTTTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGGTCCAAC‑AGGGTTTTCAAAAAAAGCAGCGGGACTGCAGGGGCCAGCGGggcg > 2:90540/1‑140 (MQ=255) cTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGC‑AGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGa < 1:808509/140‑1 (MQ=255) gCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGC‑AGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATa < 2:924100/140‑1 (MQ=255) aTTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGC‑AGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCcagc > 1:431184/1‑140 (MQ=255) aTGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGC‑AGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGaa > 1:719644/1‑140 (MQ=255) gggggTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGC‑AGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGCgg > 2:339606/1‑140 (MQ=255) | CTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGC‑AGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGCGG > NC_000913/650670‑650943 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |