Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,180,905	G→T	A271S (GCC→TCC)	yegU →	putative ADP‑ribosylglycohydrolase YegU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,180,905	0	G	T	100.0%	118.7 / NA	35	A271S (GCC→TCC)	yegU	putative ADP‑ribosylglycohydrolase YegU
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (19/16);  total (19/16)

AATCCGCCAGCGAACAGCTTTACCAGGTCGTTGGCGCAGGTACCAGCACTATTGAGTCCGTTCCGTGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCTGCGCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCATCGGTGCTATGGCGACGGCAATTTGCGGCGCGTTGCATGGCGTTAACGCTATCGATCCTGCGTTAAAGGCGGAACTGGATGCGGTAAATCAGC  >  NC_000913/2180792‑2181037                                                                                                                  |                                                                                                                                     aaTCCGCCAGCGAACAGCTTTACCAGGTCGTTGGCGCAGGTACCAGCACTATTGAGTCCGTTCCGTGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCTGCTCCGTCCTGTGCGCTAACCTTggcggc                                                                                                            <  2:263584/140‑1 (MQ=255)   tCCGCCAGCGAACAGCTTTACCAGGTCGTTGGCGCAGGTACCAGCACTATTGAGTCCGTTCCGTGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCTGCTCCGTCCTGTGCGCTAACCTTGGCGGCGa                                                                                                          >  1:761184/1‑140 (MQ=255)     cGCCAGCGAACAGCTTTACCAGGTCGTTGGCGCAGGTACCAGCACTATTGAGTCCGTTCCGTGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCTGCTCCGTCCTGTGCGCTACCCTTGGCGGCGaca                                                                                                        <  1:474332/140‑1 (MQ=255)      gCCAGCGAACAGCTTTACCAGGTCGTTGGCGCAGGTACCAGCACTATTGAGTCCGTTCCGTGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCTGCTCCGTCCTGTGCGCTAACCTTGGCGGCGacac                                                                                                       >  1:665985/1‑140 (MQ=255)             aaCAGCTTTACCAGGTCGTTGGCGCAGGTACCAGCACTATTGAGTCCGTTCCGTGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCTGCTCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACAcc                                                                                                <  2:438610/140‑1 (MQ=255)               cAGCTTTACCAGGTCGTTGGCGCAGGTACCAGCACTATTGAGTCCGTTCCGTGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCTGCTCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCAt                                                                                              <  1:962713/140‑1 (MQ=255)               cAGCTTTACCAGGTCGTTGGCGCAGGTACCAGCACTATTGAGTCCGTTCCGTGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCTGCTCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCAt                                                                                              <  2:761184/140‑1 (MQ=255)               cAGCTTTACCAGGTCGTTGGCGCAGGTACCAGCACTATTGAGTCCGTTCCGTGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCTGCTCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCAt                                                                                              <  2:836946/140‑1 (MQ=255)                          ggTCGTTGGCGCAGGTACCAGCACTATTGAGTCCGTTCCGTGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCTGCTCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCATCGGTGCTATgg                                                                                   >  1:241704/1‑140 (MQ=255)                          ggTCGTTGGCGCAGGTACCAGCACTATTGAGTCCGTTCCGTGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCTGCTCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCATCGGTGCTATgg                                                                                   >  1:425363/1‑140 (MQ=255)                           gTCGTTGGCGCAGGTACCAGCACTATTGAGTCCGTTCCGTGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCTGCTCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCATCGGTGCTATGGc                                                                                  <  2:109454/140‑1 (MQ=255)                            tCGTTGGCGCAGGTACCAGCACTATTGAGTCCGTTCCGTGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCTGCTCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCATCGGTGCTATGGCg                                                                                 >  2:696717/1‑140 (MQ=255)                                 ggCGCAGGTACCAGCACTATTGAGTCCGTTCCGTGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCTGCTCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCATCGGTGCTATGGCGACGGc                                                                            >  1:369360/1‑140 (MQ=255)                                       ggTACCAGCACTATTGAGTCCGTTCCGTGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCTGCTCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCATCGGTGCTATGGCGACGGCAATTTg                                                                      <  1:961400/140‑1 (MQ=255)                                              gCACTATTGAGTCCGTTCCGTGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCTGCTCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCATCGGTGCTATGGCGACGGCAATTTGCGGcgcg                                                               <  2:389074/140‑1 (MQ=255)                                                  tATTGAGTCCGTTCCGTGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCTGCTCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCATCGGTGCTATGGCGACGGCAATTTGCGGCGCGTTGc                                                           >  1:693822/1‑140 (MQ=255)                                                   aTTGAGTCCGTTCCGTGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCTGCTCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCATCGGTGCTATGGCGACGGCAATTTGCGGCGCGTTGCa                                                          <  2:369360/140‑1 (MQ=255)                                                    ttGAGTCCGTTCCGTGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCTGCTCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCATCGGTGCTATGGCGACGGCAATTTGCGGCGCGTTGCAt                                                         >  2:679690/1‑140 (MQ=255)                                                         tccgttccgtGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCTGCTCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCATCGGTGCTATGGCGACGGCAATTTGCGGCGCGTTGCa                                                          <  1:625260/134‑1 (MQ=255)                                                         tccgttccgtGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCTGCTCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCATCGGTGCTATGGCGACGGCAATTTGCGGCGCGTTGCa                                                          >  2:625260/1‑134 (MQ=255)                                                              tccgtGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCTGCTCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCATCGGTGCTATGGCGACGGCAATTTGCGGCGCGTTGCATGGCGTTAACg                                               >  2:952268/1‑140 (MQ=255)                                                                  tGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCTGCTCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCATCGGTGCTATGGCGACGGCAATTTGCGGCGCGTTGCATGGCGTTAACGCTAt                                           >  1:26489/1‑140 (MQ=255)                                                                  tGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCTGCTCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCATCGGTGCTATGGCGACGGCAATTTGCGGCGCGTTGCATGGCGTTAACGCTAt                                           >  2:306522/1‑140 (MQ=255)                                                                  tGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCTGCTCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCATCGGTGCTATGGCGACGGCAATTTGCGGCGCGTTGCATGGCGTTAACGCTAt                                           <  1:696717/140‑1 (MQ=255)                                                                          tGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCTGCTCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCATCGGTGCTATGGCGACGGCAATTTGCGGCGCGTTGCATGGCGTTAACGCTATCGATCCTg                                   >  1:273885/1‑140 (MQ=255)                                                                              cTGGTTGAACTGGCACAAACCGACCCGAATCGCTGCTCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCATCGGTGCTATGGCGACGGCAATTTGCGGCGCGTTGCATGGCGTTAACGCTATCGATCCTGCGtt                               <  2:693822/140‑1 (MQ=255)                                                                              cTGGTTGAACTGGCACAAACCGACCCGAATCGCTGCTCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCATCGGTGCTATGGCGACGGCAATTTGCGGCGCGTTGCATGGCGTTAACGCTATCGATCCTGCGtt                               >  1:967048/1‑140 (MQ=255)                                                                                   tGAACTGGCACAAACCGACCCGAATCGCTGCTCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCATCGGTGCTATGGCGACGGCAATTTGCGGCGCGTTGCATGGCGTTAACGCTATCGATCCTGCGTTAAAgg                          <  1:679690/140‑1 (MQ=255)                                                                                        tGGCACAAACCGACCCGAATCGCTGCTCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCATCGGTGCTATGGCGACGGCAATTTGCGGCGCGTTGCATGGCGTTAACGCTATCGATCCTGCGTTAAAGGCGGaa                     <  2:665985/140‑1 (MQ=255)                                                                                           cacaAACCGACCCGAATCGCTGCTCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCATCGGTGCTATGGCGACGGCAATTTGCGGCGCGTTGCATGGCGTTAACGCTATCGATCCTGCGTTAAAGGCGGAACTg                  >  2:159446/1‑140 (MQ=255)                                                                                           cacaAACCGACCCGAATCGCTGCTCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCATCGGTGCTATGGCGACGGCAATTTGCGGCGCGTTGCATGGCGTTAACGCTATCGATCCTGCGTTAAAGGCGGAACTg                  >  2:148482/1‑140 (MQ=255)                                                                                           cacaAACCGACCCGAATCGCTGCTCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCATCGGTGCTATGGCGACGGCAATTTGCGGCGCGTTGCATGGCGTTAACGCTATCGATCCTGCGTTAAAGGCGGAACTg                  >  2:106482/1‑140 (MQ=255)                                                                                               aaCCGACCCGAATCGCTGCTCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCATCGGTGCTATGGCGACGGCAATTTGCGGCGCGTTGCATGGCGTTAACGCTATCGATCCTGCGTTAAAGGCGGAACTGGATg              >  2:491731/1‑140 (MQ=255)                                                                                                          aTCGCTGCTCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCATCGGTGCTATGGCGACGGCAATTTGCGGCGCGTTGCATGGCGTTAACGCTATCGATCCTGCGTTAAAGGCGGAACTGGATGCGGTAAATCAg   >  2:879794/1‑140 (MQ=255)                                                                                                           tCGCTGCTCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCATCGGTGCTATGGCGACGGCAATTTGCGGCGCGTTGCATGGCGTTAACGCTATCGATCCTGCGTTAAAGGCGGAACTGGATGCGGTAAATCAGc  <  2:273885/140‑1 (MQ=255)                                                                                                                  |                                                                                                                                     AATCCGCCAGCGAACAGCTTTACCAGGTCGTTGGCGCAGGTACCAGCACTATTGAGTCCGTTCCGTGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCTGCGCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCATCGGTGCTATGGCGACGGCAATTTGCGGCGCGTTGCATGGCGTTAACGCTATCGATCCTGCGTTAAAGGCGGAACTGGATGCGGTAAATCAGC  >  NC_000913/2180792‑2181037

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑