Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,663,213	G→T	G934W (GGG→TGG)	mdtF →	multidrug efflux pump RND permease MdtF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,663,213	0	G	T	100.0%	238.0 / NA	68	G934W (GGG→TGG)	mdtF	multidrug efflux pump RND permease MdtF
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (36/32);  total (36/32)

AGCTGGTCAATTCCGTTCTCGGTGATGTTGGTTGTTCCGTTAGGCGTCGTTGGCGCATTACTGGCCACCGATCTGCGCGGCTTAAGTAATGACGTCTACTTCCAGGTTGGTTTGCTGACCACCATCGGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGATGCGTTTACGCCCAATCCTGATGACCTCTCT  >  NC_000913/3663087‑3663346                                                                                                                               |                                                                                                                                      agCTGGTCAATTCCGTTCTCGGTGATGTTGGTTGTTCCGTTAGGCGTCGTTGGCGCATTACTGGCCACCGATCTGCGCGGCTTAAGTAATGACGTCTACTTCCAGGTTGGTTTGCTGACCACCATCTGGCTTTCCGCCaa                                                                                                                          >  1:243646/1‑140 (MQ=255)   cTGGTCAATTCCGTTCTCGGTGATGTTGGTTGTTCCGTTAGGCGTCGTTGGCGCATTACTGGCCACCGATCTGCGCGGCTTAAGTAATGACGTCTACTTCCAGGTTGGTTTGCTGACCACCATCTGGCTTTCCGCCaaaa                                                                                                                        >  1:840712/1‑140 (MQ=255)   cTGGTCAATTCCGTTCTCGGTGATGTTGGTTGTTCCGTTAGGCGTCGTTGGCGCATTACTGGCCACCGATCTGCGCGGCTTAAGTAATGACGTCTACTTCCAGGTTGGTTTGCTGACCACCATCTGGCTTTCCGCCaaaa                                                                                                                        >  1:225648/1‑140 (MQ=255)   cTGGTCAATTCCGTTCTCGGTGATGTTGGTTGTTCCGTTAGGCGTCGTTGGCGCATTACTGGCCACCGATCTGCGCGGCTTAAGTAATGACGTCTACTTCCAGGTTGGTTTGCTGACCACCATCTGGCTNTCCCNCnnaa                                                                                                                        >  2:950998/1‑140 (MQ=255)     ggTCAATTCCGTTCTCGGTGCTGTTGGTTGTTCCGTTAGGCGTCGTTGGCGCATTACTGGCCACCGATCTGCGCGGCTTAAGTAATGACGTCTACTTCCAGGTTGGTTTGCTGACCACCATCTGGCTTTCCGCCAAAAAc                                                                                                                      >  1:616485/1‑140 (MQ=255)     ggTCAATTCCGTTCTCGGTGATGTTGGTTGTTCCGTTAGGCGTCGTTGGCGCATTACTGGCCACCGATCTGCGCGGCTTAAGTAATGACGTCTACTTCCAGGTTGGTTTGCTGACCACCATCTGGCTTTCCGCCAAAAAc                                                                                                                      >  1:107173/1‑140 (MQ=255)     ggTCAATTCCGTTCTCGGTGATGTTGGTTGTTCCGTTAGGCGTCGTTGGCGCATTACTGGCCACCGATCTGCGCGGCTTAAGTAATGACGTCTACTTCCAGGTTGGTTTGCTGACCACCATCTGGCTTTCCGCCAAAAAc                                                                                                                      >  2:125594/1‑140 (MQ=255)     ggTCAATTCCGTTCTCGGTGATGTTGGTTGTTCCGTTAGGCGTCGTTGGCGCATTACTGGCCACCGATCTGCGCGGCTTAAGTAATGACGTCTACTTCCAGGTTGGTTTGCTGACCACCATCTGGCTTTCCGCCAAAAAc                                                                                                                      >  1:538934/1‑140 (MQ=255)     ggTCAATTCCGTTCTCGGTGATGTTGGTTGTTCCGTTAGGCGTCGTTGGCGCATTACTGGCCACCGATCTGCGCGGCTTAAGTAATGACGTCTACTTCCAGGTTGGTTTGCTGACCACCATCTGGCTTTCCGCCAAAAAc                                                                                                                      >  2:560039/1‑140 (MQ=255)      gTCAATTCCGTTCTCGGTGATGTTGGTTGTTCCGTCAGGCGTCGTTGGCGCATTACTGGCCACCGATCTGCGCGGCTTAAGTAATGACGTCTACTTCCAGGTTGGTTTGCTGACCACCATCTGGCTTTCCGCCAAAAACg                                                                                                                     >  1:932687/1‑140 (MQ=255)      gTCAATTCCGTTCTCGGTGATGTTGGTTGTTCCGTCAGGCGTCGTTGGCGCATTACTGGCCACCGATCTGCGCGGCTTAAGTAATGACGTCTACTTCCAGGTTGGTTTGCTGACCACCATCTGGCTTTCCGCCAAAAACg                                                                                                                     <  2:932687/140‑1 (MQ=255)       tCAATTCCGTTCTCGGTGATGTTGGTTGTTCCGTTAGGCGTCGTTGGCGCATTACTGGCCACCGATCTGCGCGGCTTAAGTAATGACGTCTACTTCCAGGTTGGTTTGCTGACCACCATCTGGCTTTCCGCCAAAAACGc                                                                                                                    <  1:559686/140‑1 (MQ=255)            tCCGTTCTCGGTGATGTTGGTTGTTCCGTTAGGCGTCGTTGGCGCATTACTGGCCACCGATCTGCGCGGCTTAAGTAATGACGTCTACTTCCAGGTTGGTTTGCTGACCACCATCTGGCTTTCCGCCAAAAACGCCATcc                                                                                                               >  1:381492/1‑140 (MQ=255)                ttCTCGGTGATGTTGGTTGTTCCGTTAGGCGTCGTTGGCGCATTACTGGCCACCGATCTGCGCGGCTTAAGTAATGACGTCTACTTCCAGGTTGGTTTGCTGACCACCATCTGGCTTTCCGCCAAAAACGCCCTCCTGAt                                                                                                           >  1:663333/1‑140 (MQ=255)                ttCTCGGTGATGTTGGTTGTTCCGTTAGGCGTCGTTGGCGCATTACTGGCCACCGATCTGCGCGGCTTAAGTAATGACGTCTACTTCCAGGTTGGTTTGCTGACCACCATCTGGCTTTCCGCCAAAAACGCCATCCTGAt                                                                                                           >  2:192840/1‑140 (MQ=255)                    cGGTGATGTTGGTTGTTCCGTTAGGCGTCGTTGGCGCATTACTGGCCACCGATCTGCGCGGCTTAAGTAATGACGTCTACTTCCAGGTTGGTTTGCTGACCACCATCTGGCTTTCCGCCAAAAACGCCATCCTGATTGTc                                                                                                       <  2:22176/140‑1 (MQ=255)                               gttgttCCGTTAGGCGTCGTTGGCGCATTACTGGCCACCGATCTGCGCGGCTTAAGTAATGACGTCTACTTCCAGGTTGGTTTGCTGACCACCATCTGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGt                                                                                            <  1:575856/140‑1 (MQ=255)                                 tgttCCGTTAGGCGTCGTTGGCGCATTACTGGCCACCGATCTGCGCGGCTTAAGTAATGACGTCTACTTCCAGGTTGGTTTGCTGACCACCATCTGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTg                                                                                          <  2:381492/140‑1 (MQ=255)                                    tCCGTTAGGCGTCGTTGGCGCATTACTGGCCACCGATCTGCGCGGCTTAAGTAATGACGTCTACTTCCAGGTTGGTTTGCTGACCACCATCTGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTgaga                                                                                       <  1:967033/140‑1 (MQ=255)                                    tCCGTTAGGCGTCGTTGGCGCATTACTGGCCACCGATCTGCGCGGCTTAAGTAATGACGTCTACTTCCAGGTTGGTTTGCTGACCACCATCTGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTgaga                                                                                       <  2:840712/140‑1 (MQ=255)                                    tCCGTTAGGCGTCGTTGGCGCATTACTGGCCACCGATCTGCGCGGCTTAAGTAATGACGTCTACTTCCAGGTTGGTTTGCTGACCACCATCTGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTgaga                                                                                       >  2:167610/1‑140 (MQ=255)                                    tCCGTTAGGCGTCGTTGGCGCATTACTGGCCACCGATCTGCGCGGCTTAAGTAATGACGTCTACTTCCAGGTTGGTTTGCTGACCACCATCTGGCTTTCCGCC‑AAAACGCCATCCTGATTGTCGAATTTGCCGTTGAgat                                                                                      <  2:90361/140‑1 (MQ=255)                                      cGTTAGGCGTCGTTGGCGCATTACTGGCCACCGATCTGCGCGGCTTAAGTAATGACGTCTACTTCCAGGTTGGTTTGCTGACCACCATCTGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTgaga                                                                                       >  2:685536/1‑138 (MQ=255)                                      cGTTAGGCGTCGTTGGCGCATTACTGGCCACCGATCTGCGCGGCTTAAGTAATGACGTCTACTTCCAGGTTGGTTTGCTGACCACCATCTGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTgaga                                                                                       <  1:685536/138‑1 (MQ=255)                                        ttAGGCGTCGTTGGCGCATTACTGGCCACCGATCTGCGCGGCTTAAGTAATGACGTCTACTTCCAGGTTGGTTTGCTGACCACCATCTGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAgatgat                                                                                   >  1:414140/1‑140 (MQ=255)                                        ttAGGCGTCGTTGGCGCATTACTGGCCACCGATCTGCGCGGCTTAAGTAATGACGTCTACTTCCAGGTTGGTTTGCTGACCACCATCTGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAgatgat                                                                                   >  1:503513/1‑140 (MQ=255)                                          aGGCGTCGTTGGCGCATTACTGGCCACCGATCTGCGCGGCTTAAGTAATGACGTCTACTTCCAGGTTGGTTTGCTGACCACCATCTGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAgatg                                                                                     >  2:948850/1‑136 (MQ=255)                                          aGGCGTCGTTGGCGCATTACTGGCCACCGATCTGCGCGGCTTAAGTAATGACGTCTACTTCCAGGTTGGTTTGCTGACCACCATCTGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAgatg                                                                                     <  1:948850/136‑1 (MQ=255)                                             cgtcgtTGGCGCATTACTGGCCACCGATCTGCGCGGCTTAAGTAATGACGTCTACTTCCAGGTTGGTTTGCTGACCACCATCTGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCaga                                                                              <  2:503513/140‑1 (MQ=255)                                             cgtcgtTGGCGCATTACTGGCCACCGATCTGCGCGGCTTAAGTAATGACGTCTACTTCCAGGTTGGTTTGCTGACCACCATCTGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCaga                                                                              <  1:125594/140‑1 (MQ=255)                                                  ttGGCGCATTACTGGCCACCGATCTGCGCGGCTTAAGTAATGACGTCTACTTCCAGGTTGGTTTGCTGACCACCATCTGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCagaaagaa                                                                         >  2:648847/1‑140 (MQ=255)                                                     gcgcATTACTGGCCACCGATCTGCGCGGCTTAAGTAATGACGTCTACTTCCAGGTTGGTTTGCTGACCACCATCTGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAggg                                                                      <  2:538934/140‑1 (MQ=255)                                                         aTTACTGGCCACCGATCTGCGCGGCTTAAGTAATGACGTCTACTTCCAGGTTGGTTTGCTGACCACCATCTGGCTTTCCGCCAAAAACGCCATCCTGATTGGCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGaaaa                                                                  <  2:663333/140‑1 (MQ=255)                                                              tGGCCACCGATCTGCGCGGCTTAAGTAATGACGTCTACTTCCAGGTTGGTTTGCTGACCACCATCTGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCg                                                             <  1:903646/140‑1 (MQ=255)                                                               ggCCACCGATCTGCGCGGCTTAAGTAATGACGTCTACTTCCAGGTTGGTTTGCTGACCACCATCTGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGa                                                            <  2:573347/140‑1 (MQ=255)                                                                     cGATCTGCGCGGCTTAAGTAATGACGTCTACTTCCAGGTTGGTTTGCTGACCACCATCTGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAgg                                                      >  1:830404/1‑140 (MQ=255)                                                                     cGATCTGCGCGGCTTAAGTAATGACGTCTACTTCCAGGTTGGTTTGCTGACCACCATCTGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAgg                                                      >  1:352333/1‑140 (MQ=255)                                                                     cGATCTGCGCGGCTTAAGTAATGACGTCTACTTCCAGGTTGGTTTGCTGACCACCATCTGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAgg                                                      <  1:857311/140‑1 (MQ=255)                                                                         cTGCGCGGCTTAAGTAATGACGTCTACTTCCAGGTTGGTTTGCTGACCACCATCTGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAat                                                  <  1:192840/140‑1 (MQ=255)                                                                                 cTTAAGTAATGACGTCTACTTCCAGGTTGGTTTGCTGACCACCATCTGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAg                                          >  2:508342/1‑140 (MQ=255)                                                                                           gACGTCTACTTCCAGGTTGGTTTGCTGACCACCATCTGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGAt                                >  1:856452/1‑140 (MQ=255)                                                                                           gACGTCTACTTCCAGGTTGGTTTGCTGACCACCATCTGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGAt                                <  2:352333/140‑1 (MQ=255)                                                                                            aCGTCTACTTCCAGGTTGGTTTGCTGACCACCATCTGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGATg                               <  1:218872/140‑1 (MQ=255)                                                                                            aCGTCTACTTCCAGGTTGGTTTGCTGACCACCATCTGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGATg                               >  2:630615/1‑140 (MQ=255)                                                                                            aCGTCTACTTCCAGGTTGGTTTGCTGACCACCATCTGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGATg                               >  2:185253/1‑140 (MQ=255)                                                                                               tCTACTTCCAGGTTGGTTTGCTGACCACCATCTGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAgcg                                        <  2:884049/128‑1 (MQ=255)                                                                                               tCTACTTCCAGGTTGGTTTGCTGACCACCATCTGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAgcg                                        >  1:884049/1‑128 (MQ=255)                                                                                               tCTACTTCCAGGTTGGTTTGCTGACCACCATCTGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGATGCGt                            >  1:666977/1‑140 (MQ=255)                                                                                               tCTACTTCCAGGTTGGTTTGCTGACCACCATCTGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGATGCGt                            >  2:14342/1‑140 (MQ=255)                                                                                               tCTACTTCCAGGTTGGTTTGCTGACCACCATCTGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGATGCGt                            >  2:819240/1‑140 (MQ=255)                                                                                                 tACTTCCAGGTTGGTTTGCTGACCACCATCTGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGa                                            >  2:48769/1‑122 (MQ=255)                                                                                                 tACTTCCAGGTTGGTTTGCTGACCACCATCTGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGa                                            <  1:48769/122‑1 (MQ=255)                                                                                                 tACTTCCAGGTTGGTTTGCTGACCACCATCTGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGATGCGttt                          <  1:508342/140‑1 (MQ=255)                                                                                                 tACTTCCAGATTGGTTTGCTGACCACCATCTGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGATGCGttt                          <  2:685125/140‑1 (MQ=255)                                                                                                   cTTCCAGGTTGGTTTGCTGACCACCATCTGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGATGCGTTTAc                        >  1:134786/1‑140 (MQ=255)                                                                                                    ttCCAGGTTGGTTTGCTGACCACCATCTGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGATGCGTTTACg                       <  2:117669/140‑1 (MQ=255)                                                                                                      ccAGGTTGGTTTGCTGACCACCATCTGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGATGCGTTTACGcc                     <  1:185253/140‑1 (MQ=255)                                                                                                       cAGGTTGGTTTGCTGACCACCATCTGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGATGCGTTTACGccc                    <  1:630615/140‑1 (MQ=255)                                                                                                         ggttggttTGCTGACCACCATCTGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGATGCGTTTACGCCCaa                  <  2:243646/140‑1 (MQ=255)                                                                                                         ggttggttTGCTGACCACCATCTGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGATGCGTTTACGCCCaa                  >  2:121/1‑140 (MQ=255)                                                                                                                  gCTGACCACCATCTGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGATGCGTTTACGCCCAATCCtgatga         <  2:107173/140‑1 (MQ=255)                                                                                                                   cTGACCACCATCTGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGATGCGTTTACGCCCAATCCTGATGAc        >  1:300238/1‑140 (MQ=255)                                                                                                                      accaccATCTGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGATGCGTTTACGCCCAATCCTGATGACctc     >  2:141181/1‑140 (MQ=255)                                                                                                                        caccaTCTGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGATGCGTTTACGCCCAATCCTGATGAc        <  1:480593/135‑1 (MQ=255)                                                                                                                        caccaTCTGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGATGCGTTTACGCCCAATCCTGATGAc        >  2:480593/1‑135 (MQ=255)                                                                                                                         accaTCTGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGATGCGTTTACGCCCAATCCTGATGACctctct  <  2:414140/140‑1 (MQ=255)                                                                                                                           caTCTGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGATGCGTTTACGCCCAATCCTGATGACctctct  <  2:899269/138‑1 (MQ=255)                                                                                                                           caTCTGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGATGCGTTTACGCCCAATCCTGATGACctctct  >  1:899269/1‑138 (MQ=255)                                                                                                                               |                                                                                                                                      AGCTGGTCAATTCCGTTCTCGGTGATGTTGGTTGTTCCGTTAGGCGTCGTTGGCGCATTACTGGCCACCGATCTGCGCGGCTTAAGTAATGACGTCTACTTCCAGGTTGGTTTGCTGACCACCATCGGGCTTTCCGCCAAAAACGCCATCCTGATTGTCGAATTTGCCGTTGAGATGATGCAGAAAGAAGGGAAAACGCCGATAGAGGCAATCATCGAAGCGGCGCGGATGCGTTTACGCCCAATCCTGATGACCTCTCT  >  NC_000913/3663087‑3663346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑