Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	491,906	G→T	Q165H (CAG→CAT)	apt →	adenine phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	491,906	0	G	T	100.0%	189.2 / NA	56	Q165H (CAG→CAT)	apt	adenine phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (26/30);  total (26/30)

GACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTCGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGCCTTGTCCCGTTCCCGGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACGGGG  >  NC_000913/491769‑492036                                                                                                                                          |                                                                                                                                   gACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTCGATCTCGGCGGCGAACATCg                                                                                                                                  <  1:890132/140‑1 (MQ=255) gACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTCGATCTCGGCGGCGAACATCg                                                                                                                                  <  1:302543/140‑1 (MQ=255)  aCAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTCGATCTCGGCGGCGAACATCGt                                                                                                                                 >  1:130838/1‑140 (MQ=255)                    acgacCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTCGATCTCGGCGGCGAACATCGTCTCGAAAAACAGGGCAtt                                                                                                               <  1:21103/140‑1 (MQ=255)                    acgacCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTCGATCTCGGCGGCGAACATCGTCTCGAAAAACAGGGCAtt                                                                                                               <  1:658151/140‑1 (MQ=255)                      gacCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTCGATCTCGGCGGCGAACATCGTCTCGAAAAACAGGGCATTAc                                                                                                             <  2:768272/140‑1 (MQ=255)                       acCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTCGATCTCGGCGGCGAACATCGTCTCGAAAAACAGGGCATTAcc                                                                                                            <  2:61342/140‑1 (MQ=255)                        ccTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTCGATCTCGGCGGCGAACATCGTCTCGAAAAACAGGGCATTACCa                                                                                                           >  2:809462/1‑140 (MQ=255)                                 aaCCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTCGATCTCGGCGGCGAACATCGTCTCGAAAAACAGGGCATTACCAGCTACAGcc                                                                                                  <  2:406886/140‑1 (MQ=255)                                    cggcggCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTCGATCTCGGCGGCGAACATCGTCTCGAAAAACAGGGCATTACCAGCTACAGCCTTg                                                                                               >  1:280726/1‑140 (MQ=255)                                    cggcggCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTCGATCTCGGCGGCGAACATCGTCTCGAAAAACAGGGCATTACCAGCTACAGCCTTg                                                                                               >  2:122489/1‑140 (MQ=255)                                                 gAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTCGATCTCGGCGGCGAACATCGTCTCGAAAAACAGGGCATTACCAGCTACAGCCTTGTCCCGTTCCCggg                                                                                  <  2:567655/140‑1 (MQ=255)                                                     cGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTCGATCTCGGCGGCGAACATCGTCTCGAAAAACAGGGCATTACCAGCTACAGCCTTGTCCCGTTCCCGGGCCat                                                                              <  2:280726/140‑1 (MQ=255)                                                     cGAACGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTCGATCTCGGCGGCGAACATCGTCTCGAAAAACAGGGCATTACCAGCTACAGCCTTGTCCCGTTCCCGGGCCat                                                                              >  2:126813/1‑140 (MQ=255)                                                         cGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTCGATCTCGGCGGCGAACATCGTCTCGAAAAACAGGGCATTACCAGCTACAGCCTTGTCCCGTTCCCGGGCCattaat                                                                          >  2:950102/1‑140 (MQ=255)                                                            tAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTCGATCTCGGCGGCGAACATCGTCTCGAAAAACAGGGCATTACCAGCTACAGCCTTGTCCCGTTCCCGGGCCATTAATTAt                                                                       <  1:122489/140‑1 (MQ=255)                                                                 tGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTCGATCTCGGCGGCGAACATCGTCTCGAAAAACAGGGCATTACCAGCTACAGCCTTGTCCCGTTCCCGGGCCATTAATTATCg                                                                     >  1:366882/1‑137 (MQ=255)                                                                 tGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTCGATCTCGGCGGCGAACATCGTCTCGAAAAACAGGGCATTACCAGCTACAGCCTTGTCCCGTTCCCGGGCCATTAATTATCg                                                                     <  2:366882/137‑1 (MQ=255)                                                                   aTCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTCGATCTCGGCGGCGAACATCGTCTCGAAAAACAGGGCATTACCAGCTACAGCCTTGTCCCGTTCCCGGGCCattaatt                                                                         <  2:927561/131‑1 (MQ=255)                                                                   aTCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTCGATCTCGGCGGCGAACATCGTCTCGAAAAACAGGGCATTACCAGCTACAGCCTTGTCCCGTTCCCGGGCCattaatt                                                                         >  1:927561/1‑131 (MQ=255)                                                                   aTCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTCGATCTCGGCGGCGAACATCGTCTCGAAAAACAGGGCATTACCAGCTACAGCCTTGTCCCGTTCCCGGGCCat                                                                              <  2:236910/126‑1 (MQ=255)                                                                   aTCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTCGATCTCGGCGGCGAACATCGTCTCGAAAAACAGGGCATTACCAGCTACAGCCTTGTCCCGTTCCCGGGCCat                                                                              >  1:236910/1‑126 (MQ=255)                                                                   aTCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTCGATCTCGGCGGCGAACATCGTCTCGAAAAACAGGGCATTACCAGCTACAGCCTTGTCCCGTTCCCGGGCCATTAATTATCGCCAGt                                                                >  2:677030/1‑140 (MQ=255)                                                                    tCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTCGATCTCGGCGGCGAACATCGTCTCGAAAAACAGGGCATTACCAGCTACAGCCTTGTCCCGTTCCCGGGCCATTAATTATCGCCAGTc                                                               >  2:539923/1‑140 (MQ=255)                                                                        tcgtcTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTCGATCTCGGCGGCGAACATCGTCTCGAAAAACAGGGCATTACCAGCTACAGCCTTGTCCCGTTCCCGGGCCATTAATTATCGCCAGTCTtgt                                                           <  1:950102/140‑1 (MQ=255)                                                                         cgtcTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTCGATCTCGGCGGCGAACATCGTCTCGAAAAACAGGGCATTACCAGCTACAGCCTTGTCCCGTTCCCGGGCCATTAATTATCGCCAGTCTtgtg                                                          <  1:742764/140‑1 (MQ=255)                                                                         cgtcTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTCGATCTCGGCGGCGAACATCGTCTCGAAAAACAGGGCATTACCAGCTACAGCCTTGTCCCGTTCCCGGGCCATTAATTATCGCCAGTCTtgtg                                                          <  1:539923/140‑1 (MQ=255)                                                                           tcTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTCGATCTCGGTGGCGAACATCGTCTCGAAAAACAGGGCATTACCAGCTACAGCCTTGTCCCGTTCCCGGGCCATTAATTATCGCCAGTCTTGtgct                                                        >  1:492922/1‑140 (MQ=255)                                                                             tGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTCGATCTCGGCGGCGAACATCGTCTCGAAAAACAGGGCATTACCAGCTACAGCCTTGTCCCGTTCCCGGGCCATTAATTATCGCCAGTCTTGtgctgc                                                      >  1:798213/1‑140 (MQ=255)                                                                             tGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTCGATCTCGGCGGCGAACATCGTCTCGAAAAACAGGGCATTACCAGCTACAGCCTTGTCCCGTTCCCGGGCCATTAATTATCGCCAGTCTTGtgctgc                                                      >  1:702074/1‑140 (MQ=255)                                                                               ggtggtGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTCGATCTCGGCGGCGAACATCGTCTCGAAAAACAGGGCATTACCAGCTACAGCCTTGTCCCGTTCCCGGGCCATTAATTATCGCCAGTCTTGTGCTGccc                                                    <  1:677030/140‑1 (MQ=255)                                                                                  ggtgAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTCGATCTCGGCGGCGAACATCGTCTCGAAAAACAGGGCATTACCAGCTACAGCCTTGTCCCGTTCCCGGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACg                                                 >  2:380563/1‑140 (MQ=255)                                                                                    tgAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTCGATCTCGGCGGCGAACATCGTCTCGAAAAACAGGGCATTACCAGCTACAGCCTTGTCCCGTTCCCGGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCt                                               >  2:611244/1‑140 (MQ=255)                                                                                         tGGCTGACGCTGCGTTCATTATCAACCTGTTCGATCTCGGCGGCGAACATCGTCTCGAAAAACAGGGCATTACCAGCTACAGCCTTGTCCCGTTCCCGGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGa                                          <  1:126813/140‑1 (MQ=255)                                                                                         tGGCTGACGCTGCGTTCATTATCAACCTGTTCGATCTCGGCGGCGAACATCGTCTCGAAAAACAGGGCATTACCAGCTACAGCCTTGTCCCGTTCCCGGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGa                                          <  1:611244/140‑1 (MQ=255)                                                                                                     cGTTCATTACCAACCTGTTCGATCTCGGCGGCGAACATCGTCTCGAAAAACAGGGCATTACCAGCTACAGCCTTGTCCCGTTCCCGGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGAc                                         <  2:901139/129‑1 (MQ=255)                                                                                                     cGTTCATTACCAACCTGTTCGATCTCGGCGGCGAACATCGTCTCGAAAAACAGGGCATTACCAGCTACAGCCTTGTCCCGTTCCCGGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGAc                                         >  1:901139/1‑129 (MQ=255)                                                                                                         cATTATCAACCTGTTCGATCTCGGTGGCGAACATCGTCTCGAAAAACAGGGCATTACCAGCTACAGCCTTGTCCCGTTCCCGGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCa                          <  2:492922/140‑1 (MQ=255)                                                                                                            tATCAACCTGTTCGATCTCGGCGGCGAACATCGTCTCGAAAAACAGGGCATTACCAGCTACAGCCTTGTCCCGTTCCCGGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTc                       >  2:656155/1‑140 (MQ=255)                                                                                                               cAACCTGTTCGATCTCGGCGGCGAACATCGTCTCGAAAAACAGGGCATTACCAGCTACAGCCTTGTCCCGTTCCCGGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGc                    >  2:150748/1‑140 (MQ=255)                                                                                                                 aCCTGTTCGATCTCGGCGGCGAACATCGTCTCGAAAAACAGGGCATTACCAGCTACAGCCTTGTCCCGTTCCCGGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCt                  >  1:936913/1‑140 (MQ=255)                                                                                                                   cTGTTCGATCTCGGCGGCGAACATCGTCTCGAAAAACAGGGCATTACCAGCTACAGCCTTGTCCCGTTCCCGGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCg                <  1:656155/140‑1 (MQ=255)                                                                                                                    tGTTCGATCTCGGCGGCGAACATCGTCTCGAAAAACAGGGCATTACCAGCTACAGCCTTGTCCCGTTCCCGGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGc               >  1:853764/1‑140 (MQ=255)                                                                                                                    tGTTCGATCTCGGCGGCGAACATCGTCTCGAAAAACAGGGCATTACCAGCTACAGCCTTGTCCCGTTCCCGGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGc               >  1:909392/1‑140 (MQ=255)                                                                                                                      ttCGATCTCGGCGGCGAACATCGTCTCGAAAAACAGGGCATTACCAGCTACAGCCTTGTCCCGTTCCCGGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCt                  <  2:381295/135‑1 (MQ=255)                                                                                                                      ttCGATCTCGGCGGCGAACATCGTCTCGAAAAACAGGGCATTACCAGCTACAGCCTTGTCCCGTTCCCGGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCt                  >  1:381295/1‑135 (MQ=255)                                                                                                                      ttCGATCTCGGCGGCGAACATCGTCTCGAAAAACAGGGCATTACCAGCTACAGCCTTGTCCCGTTCCCGGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCg             >  1:322050/1‑140 (MQ=255)                                                                                                                           tctcGGCGGCGAACATCGTCTCGAAAAACAGGGCATTACCAGCTACAGCCTTGTCCCGTTCCCGGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCg                >  2:376222/1‑132 (MQ=255)                                                                                                                           tctcGGCGGCGAACATCGTCTCGAAAAACAGGGCATTACCAGCTACAGCCTTGTCCCGTTCCCGGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCg                <  1:376222/132‑1 (MQ=255)                                                                                                                           tctcGGCGGCGAACATCGTCTCGAAAAACAGGGCATTACCAGCTACAGCCTTGTCCCGTTCCCGGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTg        >  1:948948/1‑140 (MQ=255)                                                                                                                            ctcGGCGGCGAACATCGTCTCGAAAAACAGGGCATTACCAGCTACAGCCTTGTCCCGTTCCCGGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGa       <  2:798213/140‑1 (MQ=255)                                                                                                                              cggcggCGAACATCGTCTCGAAAAACAGGGCATTACCAGCTACAGCCTTGTCCCGTTCCCGGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACg     <  2:130838/140‑1 (MQ=255)                                                                                                                              cggcggCGAACATCGTCTCGAAAAACAGGGCATTACCAGCTACAGCCTTGTCCCGTTCCCGGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACg     <  1:736556/140‑1 (MQ=255)                                                                                                                                 cggcgAACATCGTCTCGAAAAACAGGGCATTCCCAGCTACAGCCTTGTCCCGTTCCCGGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACgggg  <  2:725771/140‑1 (MQ=255)                                                                                                                                 cggcgAACATCGTCTCGAAAAACAGGGCATTACGACCTTAAGCCTTGTCCCCTTCCCCGGCCATTAATTATCGCCAGTCTTGTGCTTCCCACGCTACGGACAGCACCACATGTGCATTCATCCTCGCCGTTCTGACgggg  <  2:853764/140‑1 (MQ=255)                                                                                                                                 cggcgAACATCGTCTCGAAAAACAGGGCATTACCAGCTACAGCCTTGTCCCGTTCCCGGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACgggg  <  1:809462/140‑1 (MQ=255)                                                                                                                                 cggcgAACATCGTCTCGAAAAACAGGGCATTACCAGCTACAGCCTTGTCCCGTTCCCGGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACgggg  <  1:150748/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                   GACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTCGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGCCTTGTCCCGTTCCCGGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACGGGG  >  NC_000913/491769‑492036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑