Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,516,549	G→T	S324S (TCG→TCT)	patD →	gamma‑aminobutyraldehyde dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,516,549	0	G	T	100.0%	119.6 / NA	35	S324S (TCG→TCT)	patD	gamma‑aminobutyraldehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (14/21);  total (14/21)

GTACTGCGGCTTGTCGGATCTACGCGCAAAAAGGCATTTACGATACGCTGGTGGAAAAACTGGGTGCTGCGGTGGCAACGTTAAAATCTGGTGCGCCAGATGACGAGTCTACGGAGCTTGGACCTTTAAGCTCGCTGGCGCATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCACATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTATTACTATGCGCCGACGCTGCTGGCTGGCG  >  NC_000913/1516416‑1516679                                                                                                                                      |                                                                                                                                   gTACTGCGGCTTGTCGGATCTACGCGCAAAAAGGCATTTACGATACGCTGGTGGAAAAACTGGGTGCTGCGGTGGCAACGTTAAAATCTGGTGCGCCAGATGACGAGTCTACGGAGCTTGGACCTTTAAGCTCTCTGgcg                                                                                                                              <  1:723693/140‑1 (MQ=255)       cGGCTTGTCGGATCTACGCGCAAAAAGGCATTTACGATACGCTGGTGGAAAAACTGGGTGCTGCGGTGGCAACGTTAAAATCTGGTGCGCCAGATGACGAGTCTACGGAGCTTGGACCTTTAAGCTCTCTGGCGCAtctc                                                                                                                        >  1:785877/1‑140 (MQ=255)       cGGCTTGTCGGATCTACGCGCAAAAAGGCATTTACGATACGCTGGTGGAAAAACTGGGTGCTGCGGTGGCAACGTTAAAATCTGGTGCGCCAGATGACGAGTCTACGGAGCTTGGACCTTTAAGCTCTCTGGCGCAtctc                                                                                                                        >  1:742159/1‑140 (MQ=255)       cGGCTTGTCGGATCTACGCGCAAAAAGGCATTTACGATACGCTGGTGGAAAAACTGGGTGCTGCGGTGGCAACGTTAAAATCTGGTGCGCCAGATGACGAGTCTACGGAGCTTGGACCTTTAAGCTCTCTGGCGCAtctc                                                                                                                        >  1:707877/1‑140 (MQ=255)       cGGCTTGTCGGATCTACGCGCAAAAAGGCATTTACGATACGCTGGTGGAAAAACTGGGTGCTGCGGGGGCAACGTTAAAATCTGGTGCGCCAGATGACGAGTCTACGGAGCTTGGACCTTTAAGCTCTCTGGCGCAtctc                                                                                                                        >  1:942270/1‑140 (MQ=255)       cGGCTTGTCGGATCTACGCGCAAAAAGGCATTTACGATACGCTGGTGGAAAAACTGGGTGCTGCGATGGCAACGTTAAAATCTGGTGCGCCAGATGATGAGTCTACGGAGCTTGGACCTTTAAGCTCTCTGGCGCAtctc                                                                                                                        >  2:751708/1‑140 (MQ=255)         gCTTGTCGGATCTACGCGCAAAAAGGCATTTACGATACGCTGGTGGAAAAACTGGGTGCTGCGGTGGCAACGTTAAAATCTGGTGCGCCAGATGACGAGTCTACGGAGCTTGGACCTTAAAGCTCTCTGGCGCATCTCGa                                                                                                                      >  1:733589/1‑140 (MQ=255)            tGTCGGATCTACGCGCAAAAAGGCATTTACGATACGCTGGTGGAAAAACTGGGTGCTGCGGTGGCAACGTTAAAATCTGGTGCGCCAGATGACGAGTCTACGGAGCTTGGACCTTTAAGCTCTCTGGCGCATCTCGAAcg                                                                                                                   >  1:729662/1‑140 (MQ=255)            tGTCGGATCTACGCGCAAAAAGGCATTTACGATACGCTGGTGGAAAAACTGGGTGCTGCCGAGGCAACGTTAAAATCTGGTGCGCCAGAAGACGAGTCCACGGAGCTTCGACCTTTAAGCTCTCTGGCGCATCTCGAAcg                                                                                                                   >  2:937251/1‑140 (MQ=255)                   tCTACGCGCAAAAAGGCATTTACGATACGCTGGTGGAAAAACTGGGTGCTGCGGTGGCAACGTTAAAATCTGGTGCGCCAGATGACGAGTCTACGGAGCTTGGACCTTTAAGCTCTCTGGCGCATCTCGAACGCGTCGGc                                                                                                            <  2:742159/140‑1 (MQ=255)                     tACGCGCAAAAAGGCATTTACGATACGCTGGTGGAAAAACTGGGTGCTGCGGTGGCAACGTTAAAATCTGGTGCGCCAGATGACGAGTCTACGGAGCTTGGACCTTTAAGCTCTCTGGCGCATCTCGAACGCGTCGGCaa                                                                                                          <  1:357396/140‑1 (MQ=255)                       cgcgCAAAAAGGCATTTACGATACGCTGGTGGAAAAACTGGGTGCTGCGGTGGCAACGTTAAAATCTGGTGCGCCAGATGACGAGTCTACGGAGCTTGGACCTTTAAGCTCTCTGGCGCATCTCGAACGCGTCGGCAAgg                                                                                                        <  2:729662/140‑1 (MQ=255)                         cgcAAAAAGGCATTTACGATACGCTGGTGGAAAAACTGGGTGCTGCGGTGGCAACGTTAAAATCTGGTGCGCCAGATGACGAGTCTACGGAGCTTGGACCTTTAAGCTCTCTGGCGCATCTCGAACGCGTCGGCAAGGCa                                                                                                      >  1:881282/1‑140 (MQ=255)                            aaaaaGGCATTTACGATACGCTGGTGGAAAAACTGGGTGCTGCGGTGGCAACGTTAAAATCTGGTGCGCCAGATGACGAGTCTACGGAGCTTGGACCTTTAAGCTCTCTGGCGCATCTCGAACGCGTCGGCAAGGCAGTa                                                                                                   >  1:4537/1‑140 (MQ=255)                                      ttACGATACGCTGGTGGAAAAACTGGGTGCTGCGGTGGCAACGTTAAAATCTGGTGCGCCAGATGACGAGTCTACGGAGCTTGGACCTTTAAGCTCTCTGGCGCATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGa                                                                                         >  1:845335/1‑140 (MQ=255)                                                cTGGTGGAAAAACTGGGTGCTGCGGTGGCAACGTTAAAATCTGGTGCGCCAGATGACGAGTCTACGGAGCTTGGACCTTTAAGCTCTCTGGCGCATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAgg                                                                               <  2:806639/140‑1 (MQ=255)                                                          aaCTGGGTGCTGCGGTGGCAACGTTAAAATCTGGTGCGCCAGATGACGAGTCTACGGAGCTTGGACCTTTAAGCTCTCTGGCGCATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCACATCaag                                                                     <  2:881282/140‑2 (MQ=255)                                                               ggTGCTGCGGTGGCAACGTTAAAATCTGGTGCGCCAGATGACGAGTCTACGGAGCTTGGACCTTTAAGCTCTCTGGCGCATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCACATCAAAGTGAt                                                                <  2:845335/140‑1 (MQ=255)                                                                  gctgcGGTGGCAACGTTAAAATCTGGTGCGCCAGATGACGAGTCTACGGAGCTTGGACCTTTAAGCTCTCTGGCGCATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCACATCAAAGTGATCAc                                                             <  2:243901/140‑1 (MQ=255)                                                                                  tAAAATCTGGTGCGCCAGATGACGAGTCTACGGAGCTTGGACCTTTAAGCTCTCTGGCGCATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCACATCAAAGTGATCACTGGCGGTGAAAAgcgc                                             <  1:384100/140‑1 (MQ=255)                                                                                   aaaaTCTGGTGCGCCAGATGACGAGTCTACGGAGCTTGGACCTTTAAGCTCTCTGGCGCATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCACATCAAAGTGATCACTGGCGGTGAAAAGCGCa                                            <  2:378010/140‑1 (MQ=255)                                                                                   aaaaTCTGGTGCGCCAGATGACGAGTCTACGGAGCTTGGACCTTTAAGCTCTCTGGCGCATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCACATCAAAGTGATCACTGGCGGTGAAAAGCGCa                                            <  2:4537/140‑1 (MQ=255)                                                                                         tGGTGCGCCAGATGACGAGTCTACGGAGCTTGGACCTTTAAGCTCTCTGGCGCATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCACATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTa                                      <  1:912749/140‑1 (MQ=255)                                                                                         tGGTGCGCCAGATGACGAGTCTACGGAGCTTGGACCTTTAAGCTCTCTGGCGCATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCACATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTa                                      >  1:406262/1‑140 (MQ=255)                                                                                         tGGTGCGCCAGATGACGAGTCTACGGAGCTTGGACCTTTAAGCTCTCTGGCGCATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCACATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTa                                      <  2:707877/140‑1 (MQ=255)                                                                                          ggTGCGCCAGATGACGAGTCTACGGAGCTTGGACCTTTAAGCTCTCTGGCGCATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCACATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTa                                      <  1:882490/139‑1 (MQ=255)                                                                                          ggTGCGCCAGATGACGAGTCTACGGAGCTTGGACCTTTAAGCTCTCTGGCGCATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCACATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTa                                      >  2:882490/1‑139 (MQ=255)                                                                                           gTGCGCCAGATGACGAGTCTACGGAGCTTGGACCTTTAAGCTCTCTGGCGCATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCACATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAAt                                    <  1:425034/140‑1 (MQ=255)                                                                                               gcCAGATGACGAGTCTACGGAGCTTGGACCTTTAAGCTCTCTGGCGCATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCACATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCt                                <  2:894863/140‑1 (MQ=255)                                                                                                      gACGAGTCTACGGAGCTTGGACCTTTAAGCTCTCTGGCGCATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCACATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTATTACTa                         <  2:508667/140‑1 (MQ=255)                                                                                                              tACGGAGCTTGGACCTTTAAGCTCTCTGGCGCATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCACATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTATTACTATGCGCCGa                 <  1:400502/140‑1 (MQ=255)                                                                                                                     cTTGGACCTTTAAGCTCTCTGGCGCATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCACATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTATTACTATGCGCCGACgctgct          >  1:185803/1‑140 (MQ=255)                                                                                                                     cTTGGACCTTTAAGCTCTCTGGCGCATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCACATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTATTACTATGCGCCGACgctgct          >  1:749874/1‑140 (MQ=255)                                                                                                                             tttAAGCTCTCTGGCGCATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCACATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTATTACTATGCGCCGACGCTGCTGGCTGgcg  <  2:887950/140‑1 (MQ=255)                                                                                                                             tttAAGCTCTCTGGCGCATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCACATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTATTACTATGCGCCGACGCTGCTGGCTGgcg  <  2:758186/140‑1 (MQ=255)                                                                                                                             tttAAGCTCTCTGGCGCATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCACATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTATTACTATGCGCCGACGCTGCTGGCTGgcg  <  2:967531/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                   GTACTGCGGCTTGTCGGATCTACGCGCAAAAAGGCATTTACGATACGCTGGTGGAAAAACTGGGTGCTGCGGTGGCAACGTTAAAATCTGGTGCGCCAGATGACGAGTCTACGGAGCTTGGACCTTTAAGCTCGCTGGCGCATCTCGAACGCGTCGGCAAGGCAGTAGAAGAGGCGAAAGCGACAGGGCACATCAAAGTGATCACTGGCGGTGAAAAGCGCAAGGGTAATGGCTATTACTATGCGCCGACGCTGCTGGCTGGCG  >  NC_000913/1516416‑1516679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑