breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 176,908 C→A P100Q (CCG→CAG)  erpA → iron‑sulfur cluster insertion protein ErpA
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 552,641 C→A A154S (GCC→TCC)  purE ← N(5)‑carboxyaminoimidazole ribonucleotide mutase
RA 622,247 G→T intergenic (‑58/‑53) fepD ← / → entS ferric enterobactin ABC transporter membrane subunit FebD/enterobactin exporter EntS
RA 858,282 G→T F260L (TTC→TTA ybiU ← DUF1479 domain‑containing protein YbiU
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,278,153 G→T G66V (GGC→GTC)  narK → nitrate:nitrite antiporter NarK
RA 1,502,488 C→A A11D (GCT→GAT)  ydcL → DUF3313 domain‑containing lipoprotein YdcL
RA 1,520,236 G→T intergenic (+172/‑26) curA → / → mcbR NADPH‑dependent curcumin/dihydrocurcumin reductase/DNA‑binding transcriptional dual regulator McbR
RA 1,542,316 C→A G92V (GGC→GTC)  narZ ← nitrate reductase Z subunit alpha
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
RA 1,932,104 C→T intergenic (+45/+11) purT → / ← eda phosphoribosylglycinamide formyltransferase 2/KHG/KDPG aldolase
RA 1,942,030 G→T A185D (GCC→GAC)  znuA ← Zn(2(+)) ABC transporter periplasmic binding protein
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,252,429 C→A L244M (CTG→ATG)  yeiI → putative sugar kinase YeiI
RA 2,286,879 C→A pseudogene (2036/2525 nt) yejO ← adhesin‑like autotransporter YejO
RA 2,555,334 G→T A132D (GCC→GAC)  eutK ← putative structural protein, ethanolamine utilization microcompartment
RA 2,698,702 C→A intergenic (‑152/‑57) pgpC ← / → yfhH phosphatidylglycerophosphatase C/putative DNA‑binding transcriptional regulator YfhH
RA 2,966,753 C→A S561I (AGC→ATC)  ptsP ← phosphoenolpyruvate‑protein phosphotransferase PtsP
RA 3,067,165 A→T I3I (ATT→ATA ygfI ← putative LysR‑type DNA‑binding transcriptional regulator YgfI
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,422,759 C→A N108K (AAC→AAA yhdZ → putative ABC transporter ATP‑binding subunit YhdZ
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,920,441 C→A D150Y (GAT→TAT)  atpF ← ATP synthase Fo complex subunit b
RA 4,131,593 C→A H587N (CAC→AAC)  metL → fused aspartate kinase/homoserine dehydrogenase 2
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,332,933 C→A A114S (GCC→TCC)  basS ← sensory histidine kinase BasS
RA 4,497,700 C→A pseudogene (951/1191 nt) intB → KpLE2 phage‑like element; putative integrase
RA 4,585,353 G→T A470E (GCG→GAG)  hsdR ← type I restriction enzyme EcoKI endonuclease component

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3102965 3104059 1095 18 [17] [17] 18 [mutY] [mutY]
* * ÷ NC_000913 3423785–3424234 3424530–3424241 8–746 20 [16] [16] 18 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =67 (0.860)22 (0.320) 20/244 1.9 24.1% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 79 (1.150)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)144 (1.910) 72/268 0.0 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =126 (1.620)117 (1.520) 63/274 0.1 65.0% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 1 (0.010)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)88 (1.130) 62/276 0.1 95.7% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 4 (0.050)coding (3907/4134 nt) rhsA rhs element protein RhsA