breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 204,575 T→C V28A (GTC→GCC)  rnhB → RNase HII
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 327,128 G→T R40R (CGC→CGA betA ← choline dehydrogenase
RA 404,797 C→T N365N (AAC→AAT dgcC → diguanylate cyclase DgcC
RA 675,005 G→T intergenic (‑222/‑13) leuS ← / → ybeL leucine‑‑tRNA ligase/DUF1451 domain‑containing protein YbeL
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 922,467 G→T L42I (CTA→ATA)  cspD ← DNA replication inhibitor
RA 924,668 G→T A469S (GCG→TCG)  clpA → ATP‑dependent Clp protease ATP‑binding subunit ClpA
RA 1,071,813 G→T A116E (GCG→GAG)  rutC ← putative aminoacrylate peracid reductase
RA 1,091,827 G→T L155I (CTT→ATT)  pgaA ← partially deacetylated poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine outer membrane porin
RA 1,477,029 C→T A629V (GCG→GTG)  ydbD → DUF2773 domain‑containing protein YdbD
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
RA 1,754,268 A→G G70G (GGT→GGC ydhY ← putative 4Fe‑4S ferredoxin‑type protein
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,513,328 G→T C96F (TGC→TTC)  nupC → nucleoside:H(+) symporter NupC
RA 2,534,731 C→A A222A (GCC→GCA ptsI → PTS enzyme I
RA 2,566,134 G→T P251T (CCC→ACC)  eutA ← reactivating factor for ethanolamine ammonia‑lyase EutBC
RA 2,640,043 C→A R178L (CGC→CTC)  hisS ← histidine‑‑tRNA ligase
RA 2,753,321 C→A T509T (ACC→ACA recN → DNA repair protein RecN
RA 2,962,730 C→A S12I (AGC→ATC)  ppdC ← putative YgdA
RA 3,019,683 C→A R175S (CGT→AGT)  ssnA → putative aminohydrolase
RA 3,322,290 G→T N72K (AAC→AAA secG ← Sec translocon subunit SecG
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,700,153 G→T noncoding (18/64 nt) rdlD → antisense regulatory RNA RdlD
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,270,214 G→T intergenic (+87/‑24) aphA → / → yjbQ acid phosphatase/phosphotransferase/UPF0047 protein YjbQ
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,325,158 G→T T3K (ACG→AAG)  phnC ← phosphonate ABC transporter ATP binding subunit
RA 4,497,190 G→T pseudogene (441/1191 nt) intB → KpLE2 phage‑like element; putative integrase
RA 4,524,734 G→T H93N (CAT→AAT)  yjhH ← KpLE2 phage‑like element; putative 2‑dehydro‑3‑deoxy‑D‑pentonate aldolase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3102970 3104069 1100 32 [30] [27] 31 mutY mutY
* * ÷ NC_000913 3423770–3424234 3424526–3424239 6–757 31 [26] [30] 35 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =82 (0.700)77 (0.740) 56/244 0.3 50.1% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 81 (0.780)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)133 (1.160) 78/268 0.1 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =212 (1.800)210 (1.800) 94/274 0.0 66.2% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 4 (0.030)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)145 (1.230) 67/276 0.3 96.7% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 5 (0.040)coding (3907/4134 nt) rhsA rhs element protein RhsA