breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
JC JC 151,259 IS5 (+) +4 bp coding (338‑341/597 nt) yadK ← putative fimbrial protein YadK
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 374,809 C→A G314G (GGC→GGA mhpE → 4‑hydroxy‑2‑oxovalerate aldolase
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,084,175 G→T G267V (GGC→GTC)  efeB → heme‑containing peroxidase/deferrochelatase
RA 1,109,795 C→G A154G (GCC→GGC)  opgG → osmoregulated periplasmic glucans (OPGs) biosynthesis protein G
RA 1,337,873 C→A G681G (GGC→GGA acnA → aconitate hydratase 1
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
RA 1,800,540 C→A Q33H (CAG→CAT infC ← translation initiation factor IF‑3
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
RA 1,942,811 G→T V50V (GTG→GTT znuC → Zn(2(+)) ABC transporter ATP binding subunit
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,010,669 G→T G24C (GGC→TGC)  yedL → putative acetyltransferase YedL
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,702,306 G→T P55H (CCT→CAT)  recO ← DNA repair protein RecO
RA 2,984,095 C→A A10A (GCG→GCT kduI ← 5‑dehydro‑4‑deoxy‑D‑glucuronate isomerase
RA 3,336,604 A→C I67S (ATC→AGC)  ibaG ← acid stress protein IbaG
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,814,804 G→T G104V (GGC→GTC)  slmA → nucleoid occlusion factor SlmA
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,860,232 G→T intergenic (‑116/‑51) yidK ← / → yidL putative transporter YidK/putative DNA‑binding transcriptional regulator YidL
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,276,558 C→A S363* (TCG→TAG)  pdeC → c‑di‑GMP phosphodiesterase PdeC
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,500,177 C→A pseudogene (615/1199 nt) yjgX ← KpLE2 phage‑like element; putative phosphoethanolamine transferase YjgX, N‑terminal fragment

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3102964 3104051 1088 39 [38] [36] 48 [mutY] [mutY]
* * ÷ NC_000913 3423770–3424234 3424533–3424238 5–764 39 [32] [37] 40 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =61 (0.400)199 (1.460) 85/244 0.1 75.2% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 77 (0.570)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 120780562 (0.400)80 (0.590) 51/244 0.8 54.8% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 77 (0.570)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)264 (1.770) 99/268 0.0 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =289 (1.880)256 (1.680) 95/274 0.1 63.8% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 4 (0.030)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)177 (1.150) 81/276 0.2 97.8% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 4 (0.030)coding (3907/4134 nt) rhsA rhs element protein RhsA