breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 432,286 A→G intergenic (‑273/+26) tsx ← / ← yajI nucleoside‑specific channel‑forming protein Tsx/putative lipoprotein YajI
RA 558,938 A→T intergenic (+184/‑36) sfmA → / → sfmC putative fimbrial protein SfmA/putative fimbrial chaperone SfmC
RA 792,954 G→T L279I (CTT→ATT)  modF ← ABC family protein ModF
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,163,425 G→T A56S (GCG→TCG)  thiK → thiamine kinase
RA 1,310,276 C→T intergenic (‑7/+293) kch ← / ← yciI K(+) channel Kch/protein YciI
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
RA 1,682,294 C→A P46T (CCG→ACG)  rstA → DNA‑binding transcriptional regulator RstA
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
JC 2,765,463 (TATGGCAC)6→7 intergenic (‑310/+50) abpA ← / ← yfjM CP4‑57 prophage; anti‑bacteriophage protein/CP4‑57 prophage; protein YfjM
RA 2,938,160 C→A P231P (CCC→CCA fucK → L‑fuculokinase
MC JC 3,058,051 (CAG)3→2 coding (358‑360/1233 nt) serA ← phosphoglycerate dehydrogenase
RA 3,223,176 G→T D182Y (GAC→TAC)  ebgA → evolved beta‑D‑galactosidase subunit alpha
RA 3,295,564 C→A G57G (GGC→GGA yraN → UPF0102 family protein YraN
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,366,860 G→T pseudogene (106/175 nt) yhcE → putative uncharacterized protein YhcE
RA 3,537,408 C→A R8R (CGC→CGA yhgF → putative RNA‑binding protein YhgF
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
JC JC 3,721,909 IS1 (–) +9 bp coding (712‑720/852 nt) insK → IS150 conserved protein InsB
RA 3,753,110 C→A L330L (CTG→CTT yiaV ← putative membrane fusion protein
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 4,199,534 G→T E11* (GAG→TAG)  yjaG → conserved protein YjaG
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,514,306 C→T M1M (ATG→ATA) † fecB ← ferric citrate ABC transporter periplasmic binding protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3102985 3104051 1067 24 [23] [19] 26 [mutY] [mutY]
* * ÷ NC_000913 3423785–3424234 3424536–3424238 5–752 26 [16] [23] 26 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =36 (0.370)108 (1.250) 58/244 0.2 79.8% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 23 (0.270)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 120780536 (0.370)52 (0.600) 35/244 1.0 65.5% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 23 (0.270)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)121 (1.270) 77/268 0.0 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =193 (1.970)165 (1.700) 83/274 0.0 62.9% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 3 (0.030)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)129 (1.320) 73/276 0.1 96.3% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 5 (0.050)coding (3907/4134 nt) rhsA rhs element protein RhsA