BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,715,754	G→T	Q46K (CAA→AAA)	pdxY ←	pyridoxal kinase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,715,754	0	G	T	99.1%	734.8 / ‑1.9	210	coding (136/864 nt)	pdxY	pyridoxal kinase 2
Reads supporting (aligned to +/- strand): ref base G (0/0); major base T (117/91); minor base . (1/1); total (118/92)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01

Only 100 of 213 total aligned reads displayed.

TTCACCCTGCTCCGCCGATCCCAGATAGCCACTTAATACGGCATCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTGGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATG  >  NC_000913/1715617‑1715890

ttCACCCTGCTCCGCCGATCCCAGATAGCCACTTAATACGGCATCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTtgg                                                                                                                                        >  2:2807352/1‑140 (MQ=255)

   aCCCTGCTCCGCCGATCCCAGATAGCCACTTAATACGGCATCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGgtgt                                                                                                                                     <  2:1052786/140‑1 (MQ=255)

    cccTGCTCCGCCGATCCCAGATAGCCACTTAATACGGCATCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGtgtg                                                                                                                                    >  1:691339/1‑140 (MQ=255)

     ccTGCTCCGCCGATCCCAGATAGCCACTTAATACGGCATCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGa                                                                                                                                   >  1:1251571/1‑140 (MQ=255)

     ccTGCTCCGCCGATCCCAGATAGCCACTTAATACGGCATCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGa                                                                                                                                   >  2:380704/1‑140 (MQ=255)

       tGCTCCGCCGATCCCAGATAGCCACTTAATACGGCATCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGAtt                                                                                                                                 >  1:1633723/1‑140 (MQ=255)

        gCTCCGCCGATCCCAGATAGCCACTTAATACGGCATCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGgt                                                                                                                                       <  2:235484/133‑1 (MQ=255)

        gCTCCGCCGATCCCAGATAGCCACTTAATACGGCATCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTa                                                                                                                                >  1:2519892/1‑140 (MQ=255)

        gCTCCGCCGATCCCAGATAGCCACTTAATACGGCATCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTa                                                                                                                                >  1:2066848/1‑140 (MQ=255)

         cTCCGCCGATCCCAGATAGCCACTTAATACGGCATCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAg                                                                                                                               >  1:1281977/1‑140 (MQ=255)

         cTCCGCCGATCCCAGATAGCCACTTAATACGGCATCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAg                                                                                                                               >  2:2848575/1‑140 (MQ=255)

          cccgccgATCCCAGATAGCCACTTAATACGGCATCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGa                                                                                                                              <  2:2226011/139‑1 (MQ=255)

            cgccgATCCCAGATAGCCACTTAATACGGCATCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGaaa                                                                                                                            >  1:2641248/1‑140 (MQ=255)

               cgATCCCAGATAGCCACTTAATACGGCATCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAAtt                                                                                                                         >  2:1368248/1‑140 (MQ=255)

                   cccAGATAGCCACTTAATACGGCATCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAAc                                                                                                                     >  2:1916152/1‑140 (MQ=255)

                   cccAGATAGCCACTTAATACGGCATCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAAc                                                                                                                     >  1:2890744/1‑140 (MQ=255)

                   cccAGATAGCCACTTAATACGGCATCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAAc                                                                                                                     >  2:1399028/1‑140 (MQ=255)

                   cccAGATAACCACTTAATACGGCATCACAGGTGTGTAATTTATCATTGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAAc                                                                                                                     >  2:2117787/1‑140 (MQ=255)

                           gCCACTTAATACGGCATCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCa                                                                                                             >  1:461770/1‑140 (MQ=255)

                           gCCACTTAATACGGCATCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCa                                                                                                             <  2:2519892/140‑1 (MQ=255)

                            ccACTTAATACGGCATCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAg                                                                                                            >  1:2199533/1‑140 (MQ=255)

                            ccACTTAATACGGCATCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAg                                                                                                            >  1:979182/1‑140 (MQ=255)

                            ccACTTAATACGGCATCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAg                                                                                                            >  1:1215258/1‑140 (MQ=255)

                             cACTTAATACGGCATCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGc                                                                                                           >  2:2185841/1‑140 (MQ=255)

                             cACTTAATACGGCATCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGc                                                                                                           >  1:248183/1‑140 (MQ=255)

                              aCTTAATACGGCATCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCg                                                                                                          >  1:871743/1‑140 (MQ=255)

                                ttAATACGGCATCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGc                                                                                                        >  1:2831204/1‑140 (MQ=255)

                                    tACGGCATCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGa                                                                                                    >  2:439915/1‑140 (MQ=255)

                                      cGGCATCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACg                                                                                                  >  2:525747/1‑140 (MQ=255)

                                        gCATCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGtt                                                                                                <  2:1223733/140‑1 (MQ=255)

                                          aTCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTTCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTcg                                                                                              >  2:1199264/1‑140 (MQ=255)

                                          aTCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTcg                                                                                              >  2:214830/1‑140 (MQ=255)

                                          aTCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTcg                                                                                              <  2:691339/140‑1 (MQ=255)

                                            cacaGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTcgcg                                                                                            >  1:1022562/1‑140 (MQ=255)

                                            cacaGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTcgcg                                                                                            >  2:1648446/1‑140 (MQ=255)

                                            cacaGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTcgcg                                                                                            >  1:778254/1‑140 (MQ=255)

                                                 gtgtgtAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAg                                                                                       >  1:276589/1‑140 (MQ=255)

                                                    tgtAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAggcg                                                                                    >  1:858222/1‑140 (MQ=255)

                                                      tAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCCACGGCCAGACGTTCGCGCCCAggcggc                                                                                  >  1:149278/1‑140 (MQ=255)

                                                      tAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAggcggc                                                                                  >  2:1337925/1‑140 (MQ=255)

                                                      tAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAggcggc                                                                                  >  2:472048/1‑140 (MQ=255)

                                                       aaTTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGgcggcg                                                                                 <  1:1372540/140‑1 (MQ=255)

                                                         tttATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCa                                                                               >  1:2942476/1‑140 (MQ=255)

                                                         tttATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCa                                                                               <  2:742837/140‑1 (MQ=255)

                                                           tATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATc                                                                             >  1:576304/1‑140 (MQ=255)

                                                           tATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATc                                                                             >  1:1064917/1‑140 (MQ=255)

                                                            aTCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCg                                                                            >  1:551926/1‑140 (MQ=255)

                                                            aTCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCg                                                                            >  1:2004178/1‑140 (MQ=255)

                                                              cAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCTATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGa                                                                          >  1:2738648/1‑140 (MQ=255)

                                                              cAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGa                                                                          >  1:360041/1‑140 (MQ=255)

                                                                aTGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGaaa                                                                        <  2:858222/140‑1 (MQ=255)

                                                                aTGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGaaa                                                                        <  2:45113/140‑1 (MQ=255)

                                                                aTGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGaaa                                                                        <  2:1137836/140‑1 (MQ=255)

                                                                 tGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGa                                                                          >  1:2234407/1‑137 (MQ=255)

                                                                  ggcggcAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAAct                                                                      <  2:2942476/140‑1 (MQ=255)

                                                                       cAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGcc                                                                 >  1:1001272/1‑140 (MQ=255)

                                                                       cAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGcc                                                                 >  2:1886071/1‑140 (MQ=255)

                                                                          tGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCa                                                              <  2:2717125/140‑1 (MQ=255)

                                                                          tGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCa                                                              <  1:1916152/140‑1 (MQ=255)

                                                                          tGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCa                                                              <  2:1749850/140‑1 (MQ=255)

                                                                                 cacaATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTAc                                                       >  1:1648533/1‑140 (MQ=255)

                                                                                      tttCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGcc                                                                 <  2:750702/125‑1 (MQ=255)

                                                                                      tttCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCa                                                  >  1:424894/1‑140 (MQ=255)

                                                                                      tttCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCa                                                  >  2:2646791/1‑140 (MQ=255)

                                                                                      tttCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCa                                                  <  1:2117787/140‑1 (MQ=255)

                                                                                      tttCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCa                                                  <  2:424894/140‑1 (MQ=255)

                                                                                      tttCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCa                                                  >  1:2686942/1‑140 (MQ=255)

                                                                                      tttCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCa                                                  <  1:1833735/140‑1 (MQ=255)

                                                                                      tttCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCa                                                  <  1:2862366/140‑1 (MQ=255)

                                                                                      tttCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCa                                                  <  2:20473/140‑1 (MQ=255)

                                                                                      tttCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCa                                                  <  1:525747/140‑1 (MQ=255)

                                                                                      tttCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCa                                                  <  2:2756933/140‑1 (MQ=255)

                                                                                      tttCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCa                                                  >  1:14464/1‑140 (MQ=255)

                                                                                      tttCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCa                                                  <  2:871743/140‑1 (MQ=255)

                                                                                      tttCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCa                                                  <  2:1251571/140‑1 (MQ=255)

                                                                                      tttCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCa                                                  >  1:2173113/1‑140 (MQ=255)

                                                                                        tCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATg                                                <  2:1633723/140‑1 (MQ=255)

                                                                                         cGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGa                                               >  1:2351502/1‑140 (MQ=255)

                                                                                          ggTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGAc                                              <  2:1952967/140‑1 (MQ=255)

                                                                                           gTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGAcc                                             <  2:1281977/140‑1 (MQ=255)

                                                                                                     tGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACg                                   <  1:1936336/140‑1 (MQ=255)

                                                                                                      gggCGGCATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGt                                  >  2:1452193/1‑140 (MQ=255)

                                                                                                         cggcATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTgag                               <  2:2686942/140‑1 (MQ=255)

                                                                                                         cggcATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTgag                               <  2:1382497/140‑1 (MQ=255)

                                                                                                         cggcATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTgag                               <  2:2890744/140‑1 (MQ=255)

                                                                                                            cATCACGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACt                            <  2:88886/140‑1 (MQ=255)

                                                                                                                 cGCAGCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATa                       >  1:749299/1‑140 (MQ=255)

                                                                                                                     gCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCga                   <  1:2362837/140‑1 (MQ=255)

                                                                                                                     gCCAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCga                   <  2:2234502/140‑1 (MQ=255)

                                                                                                                      ccAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCgag                  >  2:2159389/1‑140 (MQ=255)

                                                                                                                      ccAGTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCgag                  >  2:808306/1‑140 (MQ=255)

                                                                                                                         gTCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAat               <  1:139585/140‑1 (MQ=255)

                                                                                                                          tCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAata              >  1:2713244/1‑140 (MQ=255)

                                                                                                                          tCCATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAata              >  1:1332640/1‑140 (MQ=255)

                                                                                                                            cATTTGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATAtt            <  2:1001272/140‑1 (MQ=255)

                                                                                                                              tttGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATAtttt          <  2:2258679/140‑1 (MQ=255)

                                                                                                                              tttGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATAtttt          <  1:2566782/140‑1 (MQ=255)

                                                                                                                                tGCCGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTtc        >  2:1299987/1‑140 (MQ=255)

                                                                                                                                   cGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTtcatc     >  2:2584151/1‑140 (MQ=255)

                                                                                                                                   cGTATTTGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTtcatc     >  2:1419353/1‑140 (MQ=255)

TTCACCCTGCTCCGCCGATCCCAGATAGCCACTTAATACGGCATCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTGGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATG  >  NC_000913/1715617‑1715890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`