BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,609,294	G→T	E217* (GAA→TAA)	yjjG →	pyrimidine nucleotidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,609,294	0	G	T	99.0%	361.6 / ‑3.7	101	E217* (GAA→TAA)	yjjG	pyrimidine nucleotidase
Reads supporting (aligned to +/- strand): ref base G (0/1); new base T (52/48); total (52/49)
Fisher's exact test for biased strand distribution p-value = 4.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 103 total aligned reads displayed.

CTGATGGTTGGCGACACTGCCGAGTCCGATATTCTCGGTGGCATCAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTA  >  NC_000913/4609159‑4609430

cTGATGGTTGGCGACACTGCCGAGTCCGATATTCTCGGTGGCATCAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACTAACt                                                                                                                                      <  1:2328437/140‑1 (MQ=255)

    tGGTTGGCGACACTGCCGAGTCCGATATTCTCGGTGGCATCAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACATGGACCGTTTCTTCGTTACACTAACTGGag                                                                                                                                  <  1:254707/140‑1 (MQ=255)

       ttGGCGACACTGCCGAGTCCGATATTCTCGGTGGCATCAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACTAACTGGagcag                                                                                                                               <  2:1126029/140‑1 (MQ=255)

       ttGGCGACACTGCCGAGTCCGATATTCTCGGTGGCATCAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACTAACTGGagcag                                                                                                                               <  1:1575613/140‑1 (MQ=255)

         ggcaacacTGCCGAGTCCGATATTCTCGGTGGCATCAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACTAACTGGAGCAGCt                                                                                                                             >  2:1360936/5‑140 (MQ=255)

         ggCGACACTGCCGAGTCCGATATTCTCGGTGGCATCAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACTAACTGGAGCAGCt                                                                                                                             >  2:1521913/1‑140 (MQ=255)

         ggCGACACTGCCGAGTCCGATATTCTCGGTGGCATCAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACTAACTGGAGCAGCt                                                                                                                             >  1:1656052/1‑140 (MQ=255)

         ggCGACACTGCCGAGTCCGATATTCTCGGTGGCATCAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGACATCGCGCCCACCTGGACCGTTTCTTCGTTGCACTAACTGGAGCAGCt                                                                                                                             >  2:924845/1‑140 (MQ=255)

          gCGACACTGCCGAGTCCGATATTCTCGGTGGCATCAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACGCCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTc                                                                                                                            <  1:591866/140‑1 (MQ=255)

          gCGACACTGCCGAGTCCGATATTCTCGGTGGCATCAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACTAACTGGAGCAGCTc                                                                                                                            <  1:944497/140‑1 (MQ=255)

           cGACACTGCCGAGTCCGATATTCTCGGTGGCATCAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACTAACTGGAGCAGCTcc                                                                                                                           <  2:2014551/140‑1 (MQ=255)

           cGACACTGCCGAGTCCGATATTCTCGGTGGCATCAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACTAACTGGAGCAGCTcc                                                                                                                           >  2:2036407/1‑140 (MQ=255)

              cacTGCCGAGTCCGATATTCTCGGTGGCATCAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACTAACTGGAGCAGCTCCtgt                                                                                                                        <  1:2036407/140‑1 (MQ=255)

                cTGCCGAGTCCGATATTCTCGGTGGCATCAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACTAACTGGAGCAGCTCCTgtgt                                                                                                                      >  2:1358802/1‑140 (MQ=255)

                        tCCGATATTCTCGGTGGCATCAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTg                                                                                                              >  2:59160/1‑140 (MQ=255)

                        tCCGATATTCTCGGTGGCATCAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTg                                                                                                              >  1:2078196/1‑140 (MQ=255)

                          cGATATTCTCGGTGGCATCAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGAt                                                                                                            <  1:326243/140‑1 (MQ=255)

                             tatTCTCGGTGGCATCAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGc                                                                                                         >  2:1689464/1‑140 (MQ=255)

                              atTCTCGGTGGCATCAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGcc                                                                                                        <  2:1688750/140‑1 (MQ=255)

                                 ctcGGTGGCATCAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCTGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTcc                                                                                                     >  1:943593/1‑140 (MQ=255)

                                 ctcGGTGGCATCAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTcc                                                                                                     <  1:2341060/140‑1 (MQ=255)

                                 ctcGGTGGCATCAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTcc                                                                                                     <  2:2889008/140‑1 (MQ=255)

                                    ggTGGCATCAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTccccc                                                                                                  >  1:2927020/1‑140 (MQ=255)

                                         cATCAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACAGTGATTGCCTCCCCCCCGtt                                                                                             >  2:2947200/1‑140 (MQ=255)

                                          aTCAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTg                                                                                            <  2:1656052/140‑1 (MQ=255)

                                          aTCAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTg                                                                                            <  1:913544/140‑1 (MQ=255)

                                          aTCAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCACCGTTg                                                                                            >  2:890672/1‑140 (MQ=255)

                                           tCAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGa                                                                                           <  1:1358802/140‑1 (MQ=255)

                                           tCAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGa                                                                                           <  2:2785738/140‑1 (MQ=255)

                                           tCAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGa                                                                                           <  2:663002/140‑1 (MQ=255)

                                                gCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGt                                                                                      >  2:1023674/1‑140 (MQ=255)

                                                gCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGt                                                                                      >  1:2493596/1‑140 (MQ=255)

                                                    ggCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTaaaa                                                                                  >  1:245598/1‑140 (MQ=255)

                                                         gCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGcc                                                                             >  1:965837/1‑140 (MQ=255)

                                                          cGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCg                                                                            >  2:1290501/1‑140 (MQ=255)

                                                                 ctggctgAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAAtttt                                                                     <  2:2134918/140‑1 (MQ=255)

                                                                 ctggctgAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAAtttt                                                                     <  1:1203350/140‑1 (MQ=255)

                                                                    gctgAATGCACACCATCGCGAGCAACCAGAAGACATCGCGCCCACCTGGACCGTTTCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCg                                                                  <  1:924845/140‑1 (MQ=255)

                                                                                ccATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAgc                                                      <  2:2183941/140‑1 (MQ=255)

                                                                                  aTCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAgcgc                                                    >  2:1478986/1‑140 (MQ=255)

                                                                                  aTCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAgcgc                                                    >  2:2044487/1‑140 (MQ=255)

                                                                                  aTCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAgcgc                                                    >  1:1439232/1‑140 (MQ=255)

                                                                                  aTCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAgcg                                                     <  2:1561329/139‑1 (MQ=255)

                                                                                  aTCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAgcg                                                     >  1:1561329/1‑139 (MQ=255)

                                                                                   tCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAgcgcg                                                   >  2:122651/1‑140 (MQ=255)

                                                                                   tCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAgcgcg                                                   >  2:2772601/1‑140 (MQ=255)

                                                                                    cgcgAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAgcgcgg                                                  >  2:1333755/1‑140 (MQ=255)

                                                                                    cgcgAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAgcgcgg                                                  >  1:319092/1‑140 (MQ=255)

                                                                                      cgAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAgcgcggcg                                                <  1:1155185/140‑1 (MQ=255)

                                                                                        aGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAgcgcggcgcg                                              >  1:1497407/1‑140 (MQ=255)

                                                                                          cAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGAt                                            <  2:1596084/140‑1 (MQ=255)

                                                                                           aaCCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATg                                           >  2:168536/1‑140 (MQ=255)

                                                                                              cAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCg                                        <  1:122651/140‑1 (MQ=255)

                                                                                                  aGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTa                                    <  1:1790403/140‑1 (MQ=255)

                                                                                                     cATCGCGCCCACCTGGACCGTTTCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTc                                 >  2:476488/1‑140 (MQ=255)

                                                                                                       tCGCGCCCACCTGGACCGTTTCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCaa                               >  1:2077935/1‑140 (MQ=255)

                                                                                                       tCGCGCCCACCTGGACCGTTTCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCaa                               >  2:260548/1‑140 (MQ=255)

                                                                                                           gcCCACCTGGACCGTTTCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCaagaag                           >  2:909583/1‑140 (MQ=255)

                                                                                                            cccACCTGGACCGTTTCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAagaaga                          <  1:168536/140‑1 (MQ=255)

                                                                                                            cccACCTGGACCGTTTCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAagaaga                          <  1:2052233/140‑1 (MQ=255)

                                                                                                            cccACCTGGACCGTTTCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAagaaga                          <  1:1689464/140‑1 (MQ=255)

                                                                                                            cccACCTGGACCGTTTCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAagaaga                          <  2:423997/140‑1 (MQ=255)

                                                                                                            cccACCTGGACCGTTTCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAagaaga                          >  1:1620577/1‑140 (MQ=255)

                                                                                                             ccACCTGGACCGTTTCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAgaagaa                         <  1:1624694/140‑1 (MQ=255)

                                                                                                              cACCTGGACCGTTTCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGaagaaa                        >  1:377589/1‑140 (MQ=255)

                                                                                                                    gACCGTTTCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTa                  >  1:484519/1‑140 (MQ=255)

                                                                                                                       cGTTTCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGa               <  1:59160/140‑1 (MQ=255)

                                                                                                                         tttCTTCGTTGCACTAACTGGGGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACg             <  1:1402593/140‑1 (MQ=255)

                                                                                                                         tttCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAagaaga                          >  1:2627003/1‑127 (MQ=255)

                                                                                                                         tttCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAagaaga                          <  2:2627003/127‑1 (MQ=255)

                                                                                                                         tttCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAgaagaa                         >  2:1826961/1‑128 (MQ=255)

                                                                                                                         tttCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAgaagaa                         <  1:1826961/128‑1 (MQ=255)

                                                                                                                         tttCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACg             >  2:606987/1‑140 (MQ=255)

                                                                                                                         tttCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACg             <  2:2495401/140‑1 (MQ=255)

                                                                                                                         tttCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACg             <  2:484519/140‑1 (MQ=255)

                                                                                                                         tttCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACg             <  2:2493596/140‑1 (MQ=255)

                                                                                                                         tttCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACg             >  2:2035982/1‑140 (MQ=255)

                                                                                                                         tttCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACg             >  2:2196794/1‑140 (MQ=255)

                                                                                                                         tttCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACg             <  1:1290501/140‑1 (MQ=255)

                                                                                                                         tttCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACg             >  1:427019/1‑140 (MQ=255)

                                                                                                                         tttCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACg             <  1:2772601/140‑1 (MQ=255)

                                                                                                                         tttCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACg             >  1:2683445/1‑140 (MQ=255)

                                                                                                                         tttCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACg             >  1:2102216/1‑140 (MQ=255)

                                                                                                                         tttCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACg             <  1:2177530/140‑1 (MQ=255)

                                                                                                                         tttCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACg             >  1:2495401/1‑140 (MQ=255)

                                                                                                                         tttCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACg             >  2:1417955/1‑140 (MQ=255)

                                                                                                                         tttCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGGAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACg             >  2:2177530/1‑140 (MQ=255)

                                                                                                                         tttCTTCGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGAAAAATAGCCGCAATTTTTCGTTTTCAAAAAGCCCGGCGCGATGCCGCTTACTAAAAAAGAAAGAATTATGACg             >  2:1402593/1‑140 (MQ=255)

                                                                                                                          ttcttcGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGt            <  1:1360936/140‑1 (MQ=255)

                                                                                                                           tcttcGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGtt           >  2:1496032/1‑140 (MQ=255)

                                                                                                                           tcttcGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGtt           <  2:1174445/140‑1 (MQ=255)

                                                                                                                           tcttcGTTGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGtt           >  1:794131/1‑140 (MQ=255)

                                                                                                                                 ttGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTcc     <  1:1950937/140‑1 (MQ=255)

                                                                                                                                 ttGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTcc     <  1:2035982/140‑1 (MQ=255)

                                                                                                                                 ttGCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTcc     <  1:1023674/140‑1 (MQ=255)

                                                                                                                                   gCACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCtt   <  2:2102216/140‑1 (MQ=255)

                                                                                                                                    cACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCtt   <  2:1708504/139‑1 (MQ=255)

                                                                                                                                    cACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTa  >  1:1417128/1‑140 (MQ=255)

                                                                                                                                    cACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTa  <  2:2496482/140‑1 (MQ=255)

                                                                                                                                    cACTAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTa  <  1:1417955/140‑1 (MQ=255)

CTGATGGTTGGCGACACTGCCGAGTCCGATATTCTCGGTGGCATCAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTA  >  NC_000913/4609159‑4609430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`