Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,472,227	G→T	R57S (CGC→AGC)	pyrB ←	aspartate carbamoyltransferase catalytic subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,472,227	0	G	T	100.0%	293.2 / NA	81	R57S (CGC→AGC)	pyrB	aspartate carbamoyltransferase catalytic subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (44/37);  total (44/37)

CGCATCGACGTAAGTGCTGATAACCGAAATGGTATCGGCCAGCGTTTCGCCCTTTTTACCCAGTGATGTATTGGCGCTGTCGGAGAAGCCCACCACGCTGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGCGGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGG  >  NC_000913/4472090‑4472361                                                                                                                                          |                                                                                                                                       cGCATCGACGTAAGTGCTGATAACCGAAATGGTATCGGCCAGCGTTTCGCCCTTTTTACCCAGTGATGTATTGGCGCTGTCGGAGAAGCCCACCACGCTGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGctgg                                                                                                                                      >  1:142187/1‑140 (MQ=255)  gCATCGACGTAAGTGCTGATAACCGAAATGGTATCGGCCAGCGTTTCGCCCTTTTTACCCAGTGATGTATTGGCGCTGTCGGAGAAGCCCACCACGCTGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGctggt                                                                                                                                     <  2:1090145/140‑1 (MQ=255)   cATCGACGTAAGTGCTGATAACCGAAATGGTATCGGCCAGCGTTTCGCCCTTTTTACCCAGTGATGTATTGGCGCTGTCGGAGAAGCCCACCACGCTGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGctggta                                                                                                                                    >  1:348452/1‑140 (MQ=255)     tCGACGTAAGTGCTGATAACCGAAATGGTATCGGCCAGCGTTTCGCCCTTTTTACCCAGTGATGTATTGGCGCTGTCGGAGAAGCCCACCACGCTGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGctggtacg                                                                                                                                  >  2:226688/1‑140 (MQ=255)     tCGACGTAAGTGCTGATAACCGAAATGGTATCGGCCAGCGTTTCGCCCTTTTTACCCAGTGATGTATTGGCGCTGTCGGAGAAGCCCACCACGCTGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGctggtacg                                                                                                                                  >  2:1888912/1‑140 (MQ=255)     tCGACGTAAGTGCTGATAACCGAAATGGTATCGGCCAGCGTTTCGCCCTTTTTACCCAGTGATGTATTGGCGCTGTCGGAGAAGCCCACCACGCTGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGctggtacg                                                                                                                                  >  1:65252/1‑140 (MQ=255)     tCGACGTAAGTGCTGATAACCGAAATGGTATCGGCCAGCGTTTCGCCCTTTTTACCCAGTGATGTATTGGCGCTGTCGGAGAAGCCCACCACGCTGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGctggt                                                                                                                                     >  1:669809/1‑137 (MQ=255)     tCGACGTAAGTGCTGATAACCGAAATGGTATCGGCCAGCGTTTCGCCCTTTTTACCCAGTGATGTATTGGCGCTGTCGGAGAAGCCCACCACGCTGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGctggt                                                                                                                                     <  2:669809/137‑1 (MQ=255)        aCGTAAGTGCTGATAACCGAAATGGTATCGGCCAGCGTTTCGCCCTTTTTACCCAGTGATGTATTGGCGCTGTCGGAGAAGCCCACCACGCTGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGctggtacgggt                                                                                                                               >  2:439735/1‑140 (MQ=255)        aCGTAAGTGCTGATAACCGAAATGGTATCGGCCAGCGTTTCGCCCTTTTTACCCAGTGATGTATTGGCGCTGTCGGAGAAGCCCACCACGCTGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGctggtacgggg                                                                                                                               >  2:1880635/1‑139 (MQ=255)          gTAAGTGCTGATAACCGAAATGGTATCGGCCAGCGTTTCGCCCTTTTTACCCAGTGATGTATTGGCGCTGTCGGAGAAGCCCACCACGCTGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGCTGGTACGGGTag                                                                                                                             <  1:831555/140‑1 (MQ=255)          gTAAGTGCTGATAACCGAAATGGTATCGGCCAGCGTTTCGCCCTTTTTACCCAGTGATGTATTGGCGCTGTCGGAGAAGCCCACCACGCTGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGCTGGTACGGGTag                                                                                                                             <  1:1165028/140‑1 (MQ=255)          gTAAGTGCTGATAACCGAAATGGTATCGGCCAGCGTTTCGCCCTTTTTACCCAGTGATGTATTGGCGCTGTCGGAGAAGCCCACCACGCTGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGCTGGTACGGGTag                                                                                                                             <  2:1924042/140‑1 (MQ=255)              gTGCTGATAACCGAAATGGTATCGGCCAGCGTTTCGCCCTTTTTACCCAGTGATGTATTGGCGCTGTCGGAGAAGCCCACCACGCTGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGCTGGTACGGGTAGAGGc                                                                                                                         >  2:540425/1‑140 (MQ=255)                    aTAACCGAAATGGTATCGGCCAGCGTTTCGCCCTTTTTACCCAGTGATGTATTGGCGCTGTCGGAGAAGCCCACCACGCTGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGCTGGTACGGGTAGAGGCTTCgaa                                                                                                                   >  1:524433/1‑140 (MQ=255)                    aTAACCGAAATGGTATCGGCCAGCGTTTCGCCCTTTTTACCCAGTGATGTATTGGCGCTGTCGGAGAAGCCCACCACGCTGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGCTGGTACGGGTAGAGGCTTCgaa                                                                                                                   >  1:476643/1‑140 (MQ=255)                         cGAAATGGTATCGGCCAGCGTTTCGCCCTTTTTACCCAGTGATGTATTGGCGCTGTCGGAGAAGCCCACCACGCCGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGCTGGTACGGGTAGAGGCTTCGAAGAAAc                                                                                                              >  1:188357/1‑140 (MQ=255)                                  aTCGGCCAGCGTTTCGCCCTTTTTACCCAGTGATGTATTGGCGCTGTCGGAGAAGCCCACCACGCTGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCaa                                                                                                     <  1:439735/140‑1 (MQ=255)                                      gCCAGCGTTTCGCCCTTTTTACCCAGTGATGTATTGGCGCTGTCGGAGAAGCCCACCACGCTGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGAc                                                                                                 >  2:54374/1‑140 (MQ=255)                                        cAGCGTTTCGCCCTTTTTACCCAGTGATGTATTGGCGCTGTCGGAGAAGCCCACCACGCTGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACtt                                                                                               <  1:1190285/140‑1 (MQ=255)                                          gCGTTTCGCCCTTTTTACCCAGTGATGTATTGGCGCTGTCGGAGAAGCCCACCACGCTGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACtt                                                                                               <  1:1732346/138‑1 (MQ=255)                                          gCGTTTCGCCCTTTTTACCCAGTGATGTATTGGCGCTGTCGGAGAAGCCCACCACGCTGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACtt                                                                                               >  2:1732346/1‑138 (MQ=255)                                          gCGTTTCGCCCTTTTTACCCAGTGATGTATTGGCGCTGTCGGAGAAGCCCACCACGCTGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTtg                                                                                             >  2:1472199/1‑140 (MQ=255)                                          gCGTTTCGCCCTTTTTACCCAGTGATGTATTGGCGCTGTCGGAGAAGCCCACCACGCTGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGCTGGTACGGGGAGAGGGTTCGAAGAAACAGCTGGCAATGACtttt                                                                                             >  2:508467/1‑139 (MQ=255)                                           cGTTTCGCCCTTTTTACCCAGTGATGTATTGGCGCTGTCGGAGAAGCCCACCACGCTGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTtgt                                                                                            >  1:1065570/1‑140 (MQ=255)                                             tttCGCCCTTTTTACCCAGTGATGTATTGGCGCTGTCGGAGAAGCCCACCACGCTGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGc                                                                                          <  2:861235/140‑1 (MQ=255)                                                              aGTGATGTATTGGCGCTGTCGGAGAAGCCCACCACGCTGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTg                                                                         <  2:142187/140‑1 (MQ=255)                                                               gTGATGTATTGGCGCTGTCGGAGAAGCCCACCACGCTGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGCTGGGACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGc                                                                        >  2:1421490/1‑140 (MQ=255)                                                                tGATGTATTGGCGCTGTCGGAGAAGCCCACCACGCTGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGCTGGTGAGGGGCGAGGCGTCGAAAACACAACTTGCAATGCCTTTTTGCCTCAACACCTCTGGTTGCg                                                                       >  2:1437043/1‑140 (MQ=255)                                                                tGATGTATTGGCGCTGTCGGAGAAGCCCACCACGCTGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCg                                                                       >  2:1538848/1‑140 (MQ=255)                                                                tGATGTATTGGCGCTGTCGGAGAAGCCCACCACGCTGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCg                                                                       >  1:1179442/1‑140 (MQ=255)                                                                  aTGTATTGGCGCTGTCGGAGAAGCCCACCACGCTGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCggg                                                                     <  1:297658/140‑1 (MQ=255)                                                                   tGTATTGGCGCTGTCGGAGAAGCCCACCACGCTGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGt                                                                    <  1:767798/140‑1 (MQ=255)                                                                     tATTGGCGCTGTCGGAGAAGCCCACCACGCCGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGttt                                                                  <  2:188357/140‑1 (MQ=255)                                                                          gcgcTGTCGGAGAAGCCCACCACGCTGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCttt                                                             >  2:241859/1‑140 (MQ=255)                                                                          gcgcTGTCGGAGAAGCCCACCACGCTGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCttt                                                             >  2:1169943/1‑140 (MQ=255)                                                                          gcgcTGTCGGAGAAGCCCACCACGCTGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCttt                                                             >  2:1787285/1‑140 (MQ=255)                                                                          gcgcTGTCGGAGAAGCCCACCACGCTGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCttt                                                             <  1:1437043/140‑1 (MQ=255)                                                                          gcgcTGTCGGAGAAGCCCACCACGCTGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCttt                                                             >  2:579164/1‑140 (MQ=255)                                                                          gcgcTGTCGGAGAAGCCCACCACGCTGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCttt                                                             >  1:1696091/1‑140 (MQ=255)                                                                          gcgcTGTCGGAGAAGCCCACCACGCTGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCttt                                                             >  1:317427/1‑140 (MQ=255)                                                                          gcgcTGTCGGAGAAGCCCACCACGCTGGCCCCCAGGCGGGGCATAGATGTTTCGAAAGAGAGGCTGGAACGGGTAGAGGCATCGTAGAAACAGCTGGCAAATACTTTTTGCTTCAACTGATCTAGTTACGGGTTTGCctt                                                             >  2:492635/1‑140 (MQ=255)                                                                            gcTGTCGGAGAAGCCCACCACGCTGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCa                                                           >  2:1031487/1‑140 (MQ=255)                                                                            gcTGTCGGAGAAGCCCACCACGCTGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCa                                                           >  2:894773/1‑140 (MQ=255)                                                                             cTGTCGGAGAAGCCCACCACGCTGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGCTGGGACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCAg                                                          >  2:699918/1‑140 (MQ=255)                                                                               gTCGGAGAAGCCCACCACGCTGGCCCCCAGGCGGTGTATAGATGTTTCGAAAGAGAGGCTGGGACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCAGtt                                                        >  2:1864841/1‑140 (MQ=255)                                                                               gTCGGAGAAGCCCACCACGCTGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCAGtt                                                        >  1:1876233/1‑140 (MQ=255)                                                                                tCGGAGAAGCCCACCACGCTGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCAGttt                                                       >  1:942755/1‑140 (MQ=255)                                                                                  ggAGAAGCCCACCACGCTGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCAGTTTcg                                                     <  1:241859/140‑1 (MQ=255)                                                                                     gaAGCCCACCACGCTGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCAGTTTcgccg                                                  <  2:708712/140‑1 (MQ=255)                                                                                        gCCCACCACGCTGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTg                                               <  1:1356585/140‑1 (MQ=255)                                                                                            accacGCTGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGc                                           >  1:1691714/1‑140 (MQ=255)                                                                                             ccacGCTGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGcc                                          <  1:991068/140‑1 (MQ=255)                                                                                              cacGCTGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCa                                         >  2:601964/1‑140 (MQ=255)                                                                                                 gCTGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCa                                      <  1:873693/140‑1 (MQ=255)                                                                                                 gCTGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCa                                      <  1:508467/140‑1 (MQ=255)                                                                                                 gCTGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCa                                      <  1:54374/140‑1 (MQ=255)                                                                                                  cTGGCCCCCAGGCGGTGTATAGATGTTTCGAAAGAGAGGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCAc                                     <  1:1864841/140‑1 (MQ=255)                                                                                                  cTGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCAc                                     <  1:1018647/140‑1 (MQ=255)                                                                                                  cTGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCAc                                     <  1:944099/140‑1 (MQ=255)                                                                                                  cTGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCAc                                     >  1:1543140/1‑140 (MQ=255)                                                                                                  cTGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCAc                                     <  2:600336/140‑1 (MQ=255)                                                                                                  cTGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGCTGGGACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCag                                     <  2:1581545/140‑2 (MQ=255)                                                                                                   tGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGcc                                          <  1:1924961/134‑1 (MQ=255)                                                                                                   tGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGcc                                          >  2:1924961/1‑134 (MQ=255)                                                                                                   tGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCAcc                                    >  1:1793881/1‑140 (MQ=255)                                                                                                   tGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCAcc                                    <  2:1712020/140‑1 (MQ=255)                                                                                                            ggCGGTGCATAGATGTTTCGAAAGAGAGGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAgg                           <  2:475831/140‑1 (MQ=255)                                                                                                             gCGGTGCATAGATGTTTCGAAAGAGAGGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGt                          <  1:601964/140‑1 (MQ=255)                                                                                                                 tGCATAGATGTTTCGAAAGAGAGGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATc                      >  1:555691/1‑140 (MQ=255)                                                                                                                 tGCATAGATGTTTCGAAAGAGAGGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATc                      >  2:1915248/1‑140 (MQ=255)                                                                                                                 tGCATAGATGTTTCGAAAGAGAGGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATc                      >  2:1192215/1‑140 (MQ=255)                                                                                                                   cATAGATGTTTCGAAAGAGAGGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATcgc                    <  1:1169943/140‑1 (MQ=255)                                                                                                                   cATAGATGTTTCGAAAGAGAGGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATcgc                    <  2:971711/140‑1 (MQ=255)                                                                                                                       gATGTTTCGAAAGAGAGGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACt                >  2:181303/1‑140 (MQ=255)                                                                                                                           tttCGAAAGAGAGGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATc                      >  2:1617589/1‑130 (MQ=255)                                                                                                                           tttCGAAAGAGAGGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATc                      <  1:1617589/130‑1 (MQ=255)                                                                                                                           tttCGAAAGAGAGGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAgg            >  1:190986/1‑140 (MQ=255)                                                                                                                           tttCGAAAGAGAGGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAgg            >  1:1328745/1‑140 (MQ=255)                                                                                                                           tttCGAAAGAGAGGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAgg            >  2:552307/1‑140 (MQ=255)                                                                                                                           tttCGAAAGAGAGGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAgg            >  2:572686/1‑140 (MQ=255)                                                                                                                           tttCGAAAGAGAGGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAgg            >  2:16519/1‑140 (MQ=255)                                                                                                                             tCGAAAGAGAGGCTGGTGCGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTc          >  1:1723956/1‑140 (MQ=255)                                                                                                                             tCGAAAGAGAGGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTc          >  1:547507/1‑140 (MQ=255)                                                                                                                             tCGAAAGAGAGGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTc          <  1:16519/140‑1 (MQ=255)                                                                                                                                aaaGAGAGGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGtt       >  1:1066764/1‑140 (MQ=255)                                                                                                                                  agagagGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTa     >  1:554294/1‑140 (MQ=255)                                                                                                                                  agagagGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTa     <  2:1659627/140‑1 (MQ=255)                                                                                                                                  agagagGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTa     <  2:1702723/140‑1 (MQ=255)                                                                                                                                    agagGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTATGGTCATCGCGACTAAGGTCGTTTATg   <  1:226688/140‑1 (MQ=255)                                                                                                                                    agagGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATg   <  2:555691/140‑1 (MQ=255)                                                                                                                                    agagGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATg   <  2:524433/140‑1 (MQ=255)                                                                                                                                    agagGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATg   <  1:1888912/140‑1 (MQ=255)                                                                                                                                     gagGCTGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATgg  >  1:1775793/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       CGCATCGACGTAAGTGCTGATAACCGAAATGGTATCGGCCAGCGTTTCGCCCTTTTTACCCAGTGATGTATTGGCGCTGTCGGAGAAGCCCACCACGCTGGCCCCCAGGCGGTGCATAGATGTTTCGAAAGAGAGGCGGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGG  >  NC_000913/4472090‑4472361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑