breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 549,695 G→T A23A (GCG→GCT ylbF → DUF2877 domain‑containing protein YlbF
RA 558,674 G→T A155S (GCA→TCA)  sfmA → putative fimbrial protein SfmA
MC JC 570,136 Δ4,455 bp IS5‑mediated [ybcK]nmpC [ybcK], ybcL, ybcM, ylcH, ybcN, ninE, ybcO, rusA, ylcG, ybcQ, nmpC
RA 633,168 C→A A104S (GCC→TCC)  hcxA ← hydroxycarboxylate dehydrogenase A
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 922,417 G→T G58G (GGC→GGA cspD ← DNA replication inhibitor
RA 1,219,223 G→T intergenic (+22/‑378) ymgF → / → ycgH inner membrane protein that interacts with cell division proteins/putative transporter component, N‑terminal fragment
RA 1,373,729 G→T A170S (GCC→TCC)  ycjO → putative ABC transporter membrane subunit YcjO
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
RA 1,840,447 C→A S327R (AGC→AGA ynjE → molybdopterin synthase sulfurtransferase
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,818,159 G→T intergenic (‑24/+39) argZ ← / ← argY tRNA‑Arg/tRNA‑Arg
RA 2,982,040 G→A P48L (CCG→CTG)  araE ← arabinose:H(+) symporter
RA 3,323,958 G→T A38E (GCG→GAG)  glmM ← phosphoglucosamine mutase
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,477,059 C→A A121S (GCG→TCG)  fkpA ← peptidyl‑prolyl cis‑trans isomerase FkpA
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
JC JC 3,744,031 IS5 (+) +4 bp coding (289‑292/468 nt) yiaL → DUF386 domain‑containing protein YiaL
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,853,083 G→T intergenic (‑95/+35) ivbL ← / ← istR ilvBN operon leader peptide/small RNA IstR‑2
RA 4,110,792 G→T A239D (GCT→GAT)  tpiA ← triose‑phosphate isomerase
RA 4,134,963 G→T D377Y (GAC→TAC)  katG → hydroperoxidase I
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,314,040 G→T R58L (CGT→CTT)  yjdP → protein YjdP
RA 4,554,906 G→T E111* (GAG→TAG)  uxuR → DNA‑binding transcriptional repressor UxuR

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3102970 3104043 1074 18 [17] [0] 18 [mutY] [mutY]
* * ÷ NC_000913 3423759–3424234 3424537–3424238 5–779 18 [17] [17] 18 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =20 (0.270)92 (1.390) 55/244 0.1 84.5% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 16 (0.240)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 120780519 (0.250)58 (0.880) 38/244 0.4 78.0% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 16 (0.240)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)113 (1.560) 68/268 0.0 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =135 (1.810)107 (1.440) 66/274 0.0 61.1% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 2 (0.030)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)92 (1.230) 52/276 0.2 92.9% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 7 (0.090)coding (3907/4134 nt) rhsA rhs element protein RhsA